Labshake search
Citations for Roche :
401 - 450 of 2348 citations for ETS Related Transcription Factor Elf 3 ELF3 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 3 mM 1,4-Dithiothréitol (DTT) (Roche, Basel, Switzerland), 10 µM ROCK inhibitor Y27632 (ATCC® ACS-3030™ ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 protease inhibitor tablets (Roche, cat. No. 11836153001) and 1 ml BioLock (IBA ...
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bioRxiv - Biochemistry 2020Quote: ... 3 tablets of EDTA-free cOmplete inhibitor (Roche) were added to the media which was then centrifuged at 14,000 × g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 3 mg/ml Dispase II (Roche, Indianapolis, IN), and 1 mg/ml trypsin inhibitor (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... 3 tablets complete protease inhibitor EDTA free (Roche) and 1 tablet PhosSTOP (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3) Complete protease inhibitor cocktail (Roche, cat #11836153001) was included for final 1X concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... or Adenosine-5’-O-(3-thiotriphosphate) (Roche, 11162306001), 1.5 μL of TF buffer ...
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bioRxiv - Genetics 2023Quote: ... and dithiothreitol (Roche; cat. no. 3483-12-3). Lysates were rocked at 4° C for 20 min and centrifuged for 10 min at 15,000 × g ...
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bioRxiv - Microbiology 2024Quote: ... with buffer 3 or the Taq polymerase (Roche). The initial denaturation for Taq polymerase at 95 °C for 5 min was followed by 30 amplification cycles of 1 min at 95 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 U/ml dispase II (Roche Diagnostics, 4942078001), and 10 mM CaCl2 for 30 min at 37 °C with occasional shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... Complete EDTA-free protease inhibitor cocktail 3 (Roche), and PhosSTOP (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Complete EDTA-free protease inhibitor cocktail 3 (Roche), and PhosSTOP phosphatase inhibitor Cocktail (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated anti-Dig (at a dilution of 1:500; #11207733910, Roche Applied Science) and a 1:70 TSA-plus Cyanine 3 were used to detect Dig-labeled cRNA probes ...
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bioRxiv - Cancer Biology 2022Quote: ... using the gRNA_enrichment1_fw (5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTTGTG-GAAAGGACGAAACACCG-3’) and gRNA_enrichment1_rv (5’-CTACACGAC-GCTCTTCCGATCT-3’) primers and the 2x KAPA HiFi HotStart Ready Mix (Roche, Cat. No. KK2601). In a subsequent PCR ...
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bioRxiv - Microbiology 2022Quote: The V4 hypervariable region of the 16S rRNA gene was amplified with the primer pair 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3) on a Fast Start High Fidelity PCR System (Roche, PQ) using the following conditions ...
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bioRxiv - Microbiology 2024Quote: ... 1µl each of 100 µM primers Sol-PrimerA (5′-GTTTCCCACTGGAGGATA-N9-3′) and Sol-PrimerB (5′-GTTTCCCACTGGAGGATA-3′) 18 and 0.8 µl Expand High Fidelity enzyme mix (Roche, Basel, Switzerland). Reaction conditions for the PCR were ...
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bioRxiv - Microbiology 2024Quote: rpoD was then amplified using psEG30F (5’-ATYGAAATCGCCAARCG-3’) and psEG790R (5’-CGGTTGATKTCCTTGA-3’) and KAPA2G Fast Hotstart Readymix (Roche-07960956001) to generate a 736 bp product as previously described (Girard et al ...
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bioRxiv - Genomics 2024Quote: ... included 1.11 μM N7XX (5′-CAAGCAGAAGACGGCATACGAGATXXXXXXXXGTCTCGTGGGCTCGG-3′) and S5XX (5′-AATGATACGGCGACCACCGAGATCTACACXXXXXXXXTCGTCGGCAGCGTC-3′) primers (384PP_AQBP) in 2× HiFi HotStart ReadyMix (Roche, KK2602, 6RES_GPSA). Dual barcode sequences in primers are denoted by “XXXXXXXX.” Unique dual barcode combinations for each well of a 384-well plate were achieved by dispensing 16 unique N7XX barcodes across each row and 24 unique S5XX barcodes across each column ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labeled RNA probes were synthesized by in-vitro transcription using the DIG RNA labeling kit (Roche). In situ analysis was performed as described previously (Cecchetti et al. ...
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bioRxiv - Immunology 2021Quote: ... Quantitative reverse transcription PCR (qRT-PCR) was performed using an SYBR FAST universal qPCR kit (KAPA Biosystems). Predesigned KiCqStart primers for DDX58 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The in vitro transcription using the T7 RNA polymerase and the DIG RNA labelling Mix (both Roche) was performed following the supplier’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense probes were synthesized by in vitro transcription using a DIG RNA Labeling Kit (Roche). Following labeling ...
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bioRxiv - Biochemistry 2022Quote: Reverse transcription and PCR were performed on 2µg of purified RNA using the Transcriptor Reverse Transcriptase (Roche). RNA was first subjected to DNAse (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Digoxigenin-labeled RNA probes were generated by in vitro transcription using a DIG RNA Labeling Kit (Roche) and purified using Micro Bio-Spin® Chromatography Columns (BioRad) ...
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bioRxiv - Genomics 2022Quote: Real-time quantitative reverse transcription PCR (qRT-PCR) was performed using KAPA SYBR FAST (Kapa Biosystems KK4610) on a 384-well LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... the plasmid was used for in vitro RNA transcription in the presence of DIG labelling mix (Roche) and RNA Polymerase (see Table S4 ...
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bioRxiv - Cell Biology 2021Quote: ... and digoxigenin-labeled RNA probes were transcribed using a digoxigenin-UTP in vitro transcription labeling mix (Roche) and either T7 or T3 RNA polymerase (Roche) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... One thousand ng of total RNA was used for retro-transcription (RT) (Expand™ Reverse Transcriptase, Roche). In the case of mixed infections ...
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bioRxiv - Developmental Biology 2023Quote: ... Riboprobes were generated by in vitro transcription using the SP6/T7 DIG RNA labelling kit (Roche, # 11175025910) after plasmid linearization as indicated in Table S4 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reverse transcription was performed on 1 µg of total RNA using the Transcriptor Reverse Transcriptase (Roche) with random hexanucleotides primers ...
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bioRxiv - Genomics 2023Quote: Real-time quantitative reverse transcription PCR (qRT-PCR) was performed using KAPA SYBR FAST (Kapa Biosystems KK4610) on a 384-well LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... In vitro transcription was performed on the linearized template using T7 RNA polymerase (Roche DIG-labelling mix) to obtain a full-length Digoxigenin-labelled DTA riboprobe ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Reverse transcription was performed using first-strand cDNA synthesis kit for RT-PCR (Roche Diagnostics, Basel, Switzerland). qPCR was then performed on a LightCycler™ (Roche Diagnostics ...
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bioRxiv - Neuroscience 2022Quote: ... was synthesized by in vitro transcription using T7 RNA polymerase with a DIG RNA labeling kit (Roche). The template for transcription was made using PCR on zebrafish cDNA with the following primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Oligo-dT primer was used to synthesize cDNA by using Transcription First Strand cDNA synthesis kit (Roche) according to product user guide ...
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bioRxiv - Neuroscience 2022Quote: ... ISH probes were prepared by in vitro transcription with DIG RNA Labeling Mix (Roche Applied Science, #11277073910) or Fluorescein RNA Labeling mix (Roche Applied Science ...
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bioRxiv - Microbiology 2022Quote: ... and used as a template for in vitro transcription using the Digoxygenin (DIG) RNA Labeling Kit (Roche) and SP6 RNA polymerase ...
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bioRxiv - Genomics 2022Quote: ... 100 ng of RNA was used for reverse transcription (RT) using Transcriptor universal cDNA master mix (Roche) for Min6 cells or superscript III enzyme (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labeled antisense probes were synthesized using an in vitro DIG-RNA labeling transcription Kit (Roche, 11175025910) with linearized pGEM-T easy vector with each corresponding gene fragment as the templates ...
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bioRxiv - Plant Biology 2021Quote: Genomic DNA was isolated as previously described (Fischer et al., 1999) and analyzed using the Genome Sequencer FLX+ System (GS FLX+; Roche Diagnostics, Basel, Switzerland), Genome Analyzer GAIIx (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... PCR efficiency for each primer pair was determined using the LinRegPCR software (Ramakers et al., 2003) and data analysed using the LightCycler® 480 software package (Roche Applied Science). Data were normalized using GmUBI3 (Glyma20g27950 ...
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bioRxiv - Plant Biology 2020Quote: ... and was then cloned into the binary vector pGreen 0229 (Hellens et al., 2000) via restriction digestion with EcoRI and NotI enzymes (Roche Diagnostics, Mannheim, Germany). The AtD14 construct (comprising 553 bp sequence upstream of the start codon and 804 bp of the coding sequence ...
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bioRxiv - Cell Biology 2023Quote: ... probe was amplified by PCR as described previously (Cox et al., 1993) and labeled using the biotin-nick translation mix (Roche Applied Science, # 11745824910), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... RNA sequencing libraries were prepared as described previously (Ma, Fuqua et al. 2019) using the KAPA Stranded mRNA-Seq Kit (cat #KK8421, KAPA Biosystems, Wilmington, MA). The pooled libraries were sequenced in an Illumina HiSeq4000 instrument (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR analysis was conducted using anti-SARS-CoV-2 primer pairs specific to the E-gene (Corman et al. 2020) and Lightmix Modular SARS and Wuhan CoV E-gene (Roche Lifescience, Basel, Switzerland) in an ABI 7500 Fast PCR cycler (PE applied biosystems ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We prepared dual- indexed libraries (Glenn et al. 2019) for targeted enrichment using the KAPA Hyper Prep Library Kit (KAPA Biosystems, Wilmington, MA) following manufacturer’s protocols ...
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bioRxiv - Physiology 2024Quote: ... Fish were manually chased for three minutes and exposed to air for one minute before being transferred to the respirometry chamber (Roche et al., 2013). ṀO2 was continuously measured for ∼ 15 minutes to estimate MMR ...
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bioRxiv - Cancer Biology 2024Quote: ... To maintain the carryover ≤ 20% with intra-day and inter-day (2 d) CV%≤ 20% acceptable for bioanalytical assays ((Clouser-Roche et al., 2008), it was necessary to perform intermediate a post-run wash injection following each standard and sample injection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared dual-indexed libraries with custom indexes (Glenn et al. 2019) and the Kapa Biosystems Hyper Prep Plus library preparation kit (F. Hoffmann-La Roche AG, Basel, Switzerland) following the manufacturer’s protocol at one-half volume.
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bioRxiv - Cancer Biology 2020Quote: ... An overnight blunt ligation was induced for the 5’ of exon 1 and the 3’ of exon 3 (5 U T4 DNA ligase (Roche, Basel, Switzerland)) at room temperature ...