Labshake search
Citations for Roche :
451 - 500 of 2348 citations for ETS Related Transcription Factor Elf 3 ELF3 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... Real-time fluorescence-monitored quantitative PCR using the primer pair 5′-CCTATC ACCCTTGCCA-3′ and 5′-GAGGCTGTTGCTTGTG-3′ was performed on a LightCycler 96 System (Roche, Basel, Switzerland). Each reaction of 20 µL consisted of 10 µL of SYBR Green I (Roche) ...
-
bioRxiv - Developmental Biology 2020Quote: ... 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
-
bioRxiv - Cell Biology 2021Quote: ... and 3× EDTA-free complete protease inhibitor cocktail (Roche). Lysates were briefly sonicated until the protein solution was clear ...
-
bioRxiv - Cancer Biology 2022Quote: ... incubated with blocking buffer containing 3 % BSA (Roche Diagnostics), 5 % goat serum (Jackson ImmunoResearch Laboratories) ...
-
bioRxiv - Neuroscience 2020Quote: Clonazepam (CAS #1622-61-3) was bought from Roche on 2018 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, Roche), and levamisole (1359302 ...
-
bioRxiv - Biophysics 2024Quote: ... 20 mM imidazole) supplemented with 3 protease inhibitors (Roche) and then lysed by an EmulsiFlex-C3 (Avestin ...
-
bioRxiv - Developmental Biology 2023Quote: ... 5-Bromo-4-chloro- 3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 cOmplete EDTA-free protease inhibitor Cocktail tablets (Roche), pH 7.2 ...
-
bioRxiv - Microbiology 2024Quote: ... 2 µg/ml of glycerol 3-phosphate dehydrogenase (Roche) and 5 µg of cell free protein extract ...
-
bioRxiv - Molecular Biology 2024Quote: ... and blocked with 3% BSA (Roche, catalog no.10735094001) in PBS for 1 h at room temperature (for RPA2 ...
-
bioRxiv - Biochemistry 2020Quote: ... The membranes were then incubated with anti-HA HRP conjugate (3F10, Roche, 1:2000 dilution) overnight at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... except Dig-labeled probes were detected with HRP-conjugated α-Dig (Roche, 11207733910, 1:500) in conjunction with the TSA system (Molecular Probes) ...
-
bioRxiv - Plant Biology 2020Quote: ... The eluates were used for western blot analysis using anti-HA (3F10)-HRP (Roche, Switzerland) and anti-FLAG M2-HRP (SIGMA ...
-
bioRxiv - Plant Biology 2022Quote: ... HA- and FLAG-tagged proteins were immunologically detected using HRP-conjugated anti-HA 3F10 (Roche) and anti-FLAG M2 (Sigma) ...
-
bioRxiv - Immunology 2023Quote: ... Myeloperoxidase was visualized with the OmniMAP anti-Rabbit HRP and ChromoMAP DAB kit (Venata/Roche). All sections were counterstained in Harris hematoxylin for 2 min ...
-
bioRxiv - Bioengineering 2021Quote: ... Approximately 100 ng of total RNA was processed using reverse transcription kit to synthesize cDNA (Roche, Basel, Switzerland). The primer sequences used for RT-PCR analysis are listed in Table S1 ...
-
bioRxiv - Microbiology 2022Quote: ... and vRNA were generated by reverse-transcription PCR (RT-PCR) using Transcriptor First Strand cDNA Synthesis Kit (Roche). Primers with specific tags for differentiations of virus mRNA ...
-
bioRxiv - Cell Biology 2022Quote: ... Transcription was performed after manufacturer’s instructions except for the use of the DIG-labeling mixture (11277073910) from Roche. The RNA probes were diluted at 5 ng/µl in HybMix ...
-
bioRxiv - Developmental Biology 2021Quote: ... Non-radioactive antisense RNA probes were generated by in vitro transcription using DIG RNA labeling kit (Roche Diagnostics). For histological analysis sections were stained with Hematoxylin and Eosin according standard protocols.
-
bioRxiv - Plant Biology 2020Quote: ... Gene expression was quantified using real-time reverse transcription PCR (qRT-PCR) with SYBR Green and ROX (Roche). Gene quantification was performed in a Step One Plus real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The plasmid was linearized and an in vitro transcription reaction with DIG labeling mix (Roche, cat. no. 1277073) was performed as previously described [18] ...
-
bioRxiv - Microbiology 2021Quote: The RNA was subjected to reverse transcription and second strand synthesis using the cDNA synthesis system kit (Roche). The acid nucleic quantification (RNA and cDNA ...
-
bioRxiv - Cancer Biology 2022Quote: One microgram of RNA was subjected to reverse transcription with Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics). qRT-PCR reaction was carried out in 384-well format using 7900HT Fast Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Physiology 2024Quote: Digoxigenin (DIG)-labeled cRNA probes were generated by in vitro transcription using DIG RNA Labeling Mix (Roche Diagnostics), T7 RNA polymerase (Roche Diagnostics) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Probes were transcribed with TranscriptAid T7 High Yield Transcription kit (Thermo, KO441) using DIG-Label UTP (Roche,11127073910).
-
bioRxiv - Developmental Biology 2022Quote: Digoxigenin-labeled RNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics, Basel, Switzerland). In situ hybridizations were performed on 16μm cryosections that were rehydrated in 1x PBS for 5min ...
-
bioRxiv - Developmental Biology 2023Quote: Digoxigenin-labeled cRNA probes were prepared via in vitro transcription (DIG RNA labeling kit; Roche Diagnostics, Basel, Switzerland) from the Prokr2 template available from Genepaint (https://gp3.mpg.de/viewer/setInfo/MH1790/12) ...
-
bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed with 1 μg of purified RNA using Transcriptor First Strand cDNA synthesis KIT (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labeled antisense riboprobes for mouse Ccrk were synthesized by in vitro transcription using 11-digoxigenin UTPs (Roche). A Ccrk cDNA fragment for in situ hybridization probe was generated by PCR ...
-
bioRxiv - Plant Biology 2024Quote: ... The PCR product was purified and used for in vitro transcription with the DIG RNA Labeling Kit (Roche).
-
bioRxiv - Microbiology 2020Quote: ... with plasmids containing the viral genome (described in (Sutherland et al., 2018)) using X-tremeGENE™ HP DNA Transfection Reagent (Roche, Indianapolis, IN, USA). Supernatants were applied to RAW264.7 cells (ATCC ...
-
bioRxiv - Microbiology 2023Quote: ... and S-D-Bact-0785-a-A-21 (GACTACHVGGGTATCTAATCC) (Klindworth et al. 2013) together with KAPA HIFI Hot Start Ready Mix (Kapa Biosystems, Cat. No. 07958935001) containing the polymerase with proofreading activity ...
-
bioRxiv - Biophysics 2022Quote: ... Antibodies used: Mouse anti-GFP primary antibody (Roche) at 1:3,000 dilution ...
-
bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labelled antisense and sense RNA probes were synthesized by in vitro transcription using DIG-labelled UTP according to the manufacturer’s instructions (DIG RNA labelling kit, Roche). Primers used were as follow ...
-
bioRxiv - Neuroscience 2022Quote: ... In vitro transcription of antisense and sense probes was performed with a DIG-RNA Labelling Mix (Roche, Mannheim, Germany) using either T7 or T3 polymerase.
-
bioRxiv - Developmental Biology 2022Quote: ... These were synthesised by in vitro transcription with DIG-RNA labelling Kit or Fluorescein-RNA labelling kit (Roche, Switzerland). The integrity of probes was determined by gel electrophoresis and storage in 50% RNAlater (Sigma ...
-
bioRxiv - Developmental Biology 2020Quote: ... DIG-labeled probes where generated using T7/SP6 transcriptions start sites and the digoxigenin RNA Labeling Mix kit (Roche) after manufactures instructions ...
-
bioRxiv - Immunology 2020Quote: DNase treatment was performed prior to Reverse transcription polymerase chain reaction (RT-PCR) using RNAse-free DNase I (Roche) at 37°C for 10 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... Half of the reverse transcription reaction was used for amplification with a KAP real-time PCR mixture (KAPA Biosystems) using the Illumina Truseq small RNA library amplification kit primers ...
-
bioRxiv - Plant Biology 2022Quote: ... 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche) and 1 µl of RNase A/T1 (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... were produced with DIG-UTP by in vitro transcription using the Sp6 and T7 promoters according to the manufacturer’s protocol (RNA-labeling kit; Roche). The sections were treated as follows ...
-
bioRxiv - Biophysics 2022Quote: ... The transcription mixture was incubated at 37 °C for 4 h and treated with 50U/mL DNAse I (Roche) at 37 °C for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... In vitro transcription (IVT) was performed using T7 mMassage mMachine kit (invitrogen) and purified using RNA purification column (Roche). MCU-IVT was injected at single cell stage and embryos were cultured in E3 water ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 20 ng of cDNA was used for quantitative reverse transcription PCR with the FastStart SYBR Green Master (Roche) and the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin labeled antisense riboprobes were synthesized by in vitro transcription using a DIG RNA labeling kit (Roche, Cat#11277073910), followed by purification using Micro Bio-Spin P-30 gel columns (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: Quantitative reverse transcription PCR (RT-qPCR) assays were performed by standard protocol suitable for LightCycler® 480 Instrument (Roche). In brief ...
-
bioRxiv - Developmental Biology 2024Quote: ... followed by transcription with T7 RNA polymerase and a nucleotide mixture containing digoxigenin-labeled or fluorescein-labeled UTP (Roche), resuspended in formamide and examined by agarose gel electrophoresis.
-
bioRxiv - Cancer Biology 2024Quote: Digoxogenin labeled cRNA probes were generated by in vitro transcription of 1µg template DNA with the RNA DIG labeling kit (#11175025910, Roche) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate (BCIP, Roche).