Labshake search
Citations for Roche :
4401 - 4450 of 5389 citations for QuantiChrom Magnesium Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The dry contents were resuspended by addition of 7.5 µl hybridization buffer and 3 µl hybridization component A (SeqCap EZ Hybridization and Wash Kit: 05 634 261 001, Roche), mixed by tapping ...
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bioRxiv - Genomics 2019Quote: ... per sample were washed twice with 200 µl 1x wash buffer (SeqCap EZ Hybridization and Wash Kit: 05 634 261 001, Roche), once with 100 µl ...
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bioRxiv - Genomics 2019Quote: ... Non-target DNA was removed through washing the beads with buffers of the SeqCap EZ Hybridization and Wash Kit (05 634 261 001, Roche) as per manufacturers instructions with the following modifications ...
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bioRxiv - Biochemistry 2019Quote: ... and S278E amino acid substitutions were introduced into human RACK1 using the QuickChange II XL site-directed mutagenesis kit (Roche). All plasmids are available upon request.
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bioRxiv - Evolutionary Biology 2019Quote: ... Libraries were subsequently index-amplified for 20 cycles using a KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, Ma. USA) in 50-μl reactions following the manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2019Quote: We prepared sequencing libraries for the ten de novo sequenced isolates using the Kapa Biosystems kit (Kapa Biosystems, Woburn, MA) and ~1μg of DNA of each isolate according to manufacturer’s protocols ...
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bioRxiv - Genetics 2019Quote: ... 1 ug purified digested plasmid was used as template for SP6 or T7 transcription using the DIG-RNA labeling kit (Roche). Following probe transcription ...
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bioRxiv - Biochemistry 2019Quote: ... RNA-seq libraries were prepared using the KAPA Stranded mRNA-Seq Kit (KAPA Biosystems, Wilmington, MA, USA, Cat. No. KK8421) from 250 ng of total RNA ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescent probes with BAC DNAs were prepared by nick translation with dATP conjugated with fluorophore or DIG using Nick Translation kit (Roche) or PCR ...
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bioRxiv - Genomics 2019Quote: ... followed by quantitation using real-time PCR using the KAPA Library Quant Kit for Illumina (KAPA Biosystems catalog no. KK4835). Libraries were then pooled and sequenced on the Illumina NextSeq 500 platform using Illumina sequencing reagents according to manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: ... Amplified genomic DNA (70 ng) was used to generate exome sequence libraries using the Hyper Prep kit (Kapa Biosystems, KK8504), SureSelect Target Enrichment kit (Agilent Technologies ...
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bioRxiv - Genetics 2019Quote: ... located in exon 18 and reverse primer 5’-TTGGTGGCTACAAAGACGTG-3’ located in exon 22 using the One Tube reverse transcription-PCR reaction kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR analysis of cytokine related porcine gene expression was performed using the KAPA SYBR-FAST qPCR Kit (KAPA Biosystems), using the primers shown in Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... and viability was measured using the Roche MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) kit (Roche, Cat # 11465007001) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: Total DNA was isolated from L3 larvae or 5-days old adults of Iduna homozygous mutants and control sequencing strain using the Roche genomic DNA extraction kit (Roche). To confirm the mutant line ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were hybridized with digoxigenin-labeled antisense GUARDIN probes synthesized using T7 RNA polymerase using the DIG Northern Starter Kit (Roche).
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bioRxiv - Cell Biology 2019Quote: ... 1μg of total RNA was subjected to reverse transcription with random primers using Takera’s TranscriptorFirst Strand cDNA Synthesis Kit and cDNA was subjected to real-time PCR analyses using LightCycler 480 SYBR Green I Master Mix (Roche) in a LightCycler 480 Instrument(BD ...
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bioRxiv - Systems Biology 2019Quote: The relative concentration of the resulting DNA ligated-probes was assessed by quantitative PCR (qPCR) using the LightCycler 480 SYBR Green I Master kit (Roche). 3.5 µl of diluted samples was used to assemble a 10 µl reaction and run on a Light Cycler 480 II (Roche ...
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bioRxiv - Systems Biology 2019Quote: ... and libraries were quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems, Wilmington MA) and sequenced on Illumina HiSeq 2500 (Illumina ...
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bioRxiv - Pathology 2020Quote: ... Total and small RNA (containing microRNA) were extracted from MΦ using the Roche High Pure miRNA Isolation kit (Roche, Milan) or the Quick-RNA MicroPrep from Zymo Research (Irvine ...
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bioRxiv - Genomics 2019Quote: The RNASeq libraries were prepared from total RNA using KAPA Stranded mRNA-Seq Kit Illumina® Platforms (Roche-Kapa Biosystems) with minor modifications ...
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bioRxiv - Genomics 2019Quote: The RNASeq libraries were prepared from total RNA using KAPA Stranded mRNA-Seq Kit Illumina® Platforms (Roche-Kapa Biosystems) with minor modifications ...
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bioRxiv - Cell Biology 2020Quote: ... Stranded RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) and KAPA Unique Dual-Indexed Adapters (Roche) according to instructions provided by the manufacturer ...
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bioRxiv - Cancer Biology 2019Quote: ... using the Agilent Bioanalyzer while library concentration was quantified by qPCR using the KAPA SYBR FAST qPCR kit (Kapa Biosystems). Libraries were normalized to 10nM ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of each library was determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce appropriate cluster counts Illumina NovaSeq6000 instrument ...
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bioRxiv - Microbiology 2021Quote: Alkali-labile digoxigenin labelled deoxyuridine triphosphate (DIG-dUTP) was incorporated in Southern blot probes by PCR using PCR DIG Probe Synthesis kit (Roche) with the following specifications (refer to sequences in Supplementary table 3) ...
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bioRxiv - Plant Biology 2021Quote: ... A PCR master mix was prepared with the LightCycler 480 SYBR Green I Master Kit (Roche Applied Science, Mannheim, Germany). Each reaction was performed in a total volume of 10 μL ...
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bioRxiv - Systems Biology 2019Quote: ... Then one hundred nanograms of RNA per sample were used to prepare dual-indexed strand-specific cDNA libraries using the KAPA mRNA HyperPrep kit (Roche). The resulting libraries were assessed for quantity and size distribution using Qubit and Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: The cell viability of circPSD3 siRNA treated cells was determined at day 2 post-transfection using Cell Proliferation Kit I (MTT, Roche) as previously described [59].
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bioRxiv - Molecular Biology 2021Quote: Serum albumin measurements were determined using a commercial serum albumin kit (Alpha Laboratories Ltd., Eastleigh, UK) adapted for use on Cobas Mira analyser (Roche Diagnostics Ltd ...
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bioRxiv - Developmental Biology 2021Quote: Genomic DNA was extracted from 3 week old tail or ear biopsies using KAPA Mouse DNA Extraction Kit (KAPA Biosystems) or High Pure PCR Template Kit (Roche).
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bioRxiv - Cancer Biology 2020Quote: ... Exon 1 and exon 3 digestion products were purified using the High pure PCR product purification kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Labelling efficiencies were tested by dot blotting with anti-digoxigenin alkaline phosphatase conjugate and CSPD chemiluminescence substrate for alkaline phosphatase from DIG High Prime DNA Labelling and Detection Starter Kit II (Roche) according to the manufacturer`s protocol ...
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bioRxiv - Immunology 2020Quote: ... 2.7 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK 8504). Samples were barcoded and used for 50bp/50bp paired end runs with the TruSeq SBS Kit v4 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using a Bio-Rad sequence detection system according to the manufacturer’s instructions using a double-stranded DNA-specific SYBR Green Premix Ex TaqTM II Kit (Roche, Switzerland). Experiments were conducted in duplicate in three independent assays ...
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bioRxiv - Molecular Biology 2020Quote: ... Intact RNA was depleted of rRNA and prepared for sequencing using the KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems). Libraries were sequenced on the NextSeq 500 (Illumina) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Library quality control and quantification were performed using the KAPA Library Quantification Kit for Illumina platforms (Kapa Biosystems, Massachusetts, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... TUNEL staining was conducted following manufacturer’s protocol (In Situ Cell Death Detection Kit, Version 17, Roche Diagnistics GmbH, Mannheim, Germany). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µg of total RNA was converted to a sequence library using a KAPA Stranded mRNA-seq Kit (KAPA Biosystems). These libraries were analyzed using a HiSeq 2500 sequencer (Illumina ...
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bioRxiv - Developmental Biology 2019Quote: ... data in the KS cohort was performed on peripheral blood-derived DNA using either Nimblegen SeqCap target enrichment kit (Roche) or a custom Illumina capture kit (ICE ...
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bioRxiv - Cancer Biology 2019Quote: ... Nucleosomal DNA was isolated and either used as a template for qPCR (ChIP-qPCR) or to generate NGS libraries using KAPA Hyper Prep Kits (Roche) and TruSeq adaptors (Bioo Scientific ...
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bioRxiv - Genomics 2021Quote: ... 10.6 kbp and 16.4 kbp runs were constructed using the Oxford Nanopore Technologies (ONT) SQK-LSK109 kit according to the manufacturer’s instructions except that Kapa beads (Roche, UK) were used to perform the clean up steps rather than Ampure XP beads ...
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bioRxiv - Genetics 2020Quote: ... One short-insert WGS library was constructed using the Kapa Hyper Prep Kit (KR0961) from Kapa Biosystems (Woburn, MA, USA) with 150 ng of sheared DNA as input following standard manufacturer’s protocol with 10 cycles of PCR ...
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bioRxiv - Physiology 2021Quote: ... All mice were genotyped by polymerase chain reaction (PCR) using KAPA Taq PCR Kits (Kapa Biosystems, Inc., Wilmington, MA, USA). Specific primers purchased from Integrated DNA Technologies (Coralville ...
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bioRxiv - Physiology 2020Quote: 1 μg RNA was reverse transcribed into cDNA with Oligo(d)T primers using the Transcriptor First Strand cDNA Synthesis kit (Roche). The qRT-PCR was performed with the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Physiology 2021Quote: ... cDNA libraries were validated using high sensitivity DNA chip on the Agilent 2100 Bioanalyzer and quantified using the KAPA Library Quantification Kit (Roche). Indexed libraries were pooled and sequenced with a 50-bp single-end protocol on a HiSeq4000 platform (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... Only one sample was obtained per patient: one portion of the sample was extracted using the High Pure Viral RNA Kit (Roche), and another portion of the same sample was extracted using the acid pH method ...
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bioRxiv - Microbiology 2020Quote: ... Total RNAs were extracted from 200 μl of mice sera using a High Pure viral RNA kit (Roche Applied Science) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were generated by random shearing of genomic DNA followed by ligation of custom adapters using the Kapa HyperPrep Kit (Roche). Briefly ...