Labshake search
Citations for Roche :
4501 - 4550 of 5389 citations for QuantiChrom Magnesium Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Digoxigenin (DIG)-labeled RNA probes were transcribed in vitro using a DIG RNA labeling kit (Roche Applied Science, Basel, Switzerland) and brain sections were hybridized with gene-specific antisense RNA probes ...
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bioRxiv - Cancer Biology 2022Quote: ... and 500 ng of DNA-free RNA was reverse transcribed using the First Strand cDNA Transcriptor Synthesis Kit (Roche Life Science, Roche Diagnostics Division ...
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bioRxiv - Cancer Biology 2022Quote: ... the DNA fragments were “End-Repaired,” “A-tailed,” and the index adapters were ligated (Agencourt AMPure XP Beads; SeqCap EZ Pure Capture Bead Kit; Hoffmann-La Roche). Following that ...
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bioRxiv - Developmental Biology 2022Quote: 155BS-upd from Doug Harrison and RE35237 from BDGP cDNAs were used to generate upd and sna RNA probes using the DIG RNA Labeling Kit (Roche). Secondary biotinylated antibody against mouse (1:200 ...
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bioRxiv - Genomics 2022Quote: ... RNA Libraries were prepared from 100 ng of total RNA with the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems KK8401). Indexed libraries were then pooled and 2×50 bp ...
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bioRxiv - Genomics 2022Quote: ... and P5 and P7 adapters were ligated per pool following the KAPA HiFi Hotstart PCR kit standard protocol (Kapa Biosystems). Pooled samples were then combined into one sample and cleaned as before ...
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bioRxiv - Plant Biology 2022Quote: ... Sense and anti-sense digoxigenin-labelled probes were synthesised using the DIG RNA Labeling Kit (SP6/T7; Roche, Basel, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the ligated product was amplified by 12 PCR cycles using the Kapa Hifi Hotstart NGS library amplification kit (Kapa Biosystems), followed by purification with 0.6×AMPure XP ...
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bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
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bioRxiv - Plant Biology 2022Quote: ... The RNA level was detected using the DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Cat. 11585614910).
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bioRxiv - Microbiology 2022Quote: ... was collected from infected organoids for HBeAg and HBsAg quantification using the Elecsys HBeAg and HBsAg II kit chemiluminescent microparticle immunoassay (CMIA) on the Cobas e602 instrument (Roche).
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... The antisense RNA probes corresponding to the 3’ s-end portion of glnA CDS were prepared by the DIG RNA Labeling Kit (Roche).
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bioRxiv - Plant Biology 2022Quote: ... derived from SlARF2A and SlARF2B promoter was labeled with digoxigenin as per the manufacturer’s protocol using DIG Oligonucleotide 3′-End Labeling Kit (Roche Diagnostics). The same unlabeled DNA fragment was used as a competitor ...
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bioRxiv - Plant Biology 2022Quote: ... derived from SlGLYI4 promoter was labelled with digoxigenin as per the manufacturer’s protocol using DIG Oligonucleotide 3′- End Labelling Kit (Roche Diagnostics). The same unlabelled DNA fragment was used as a competitor ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). Primers used in the RT-qPCR are listed in Supplementary Table S1 ...
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bioRxiv - Neuroscience 2023Quote: ... 2008) using commercial transcription kits and RNA labeling mix (REF #: 11277073910, Lot #: 26591021 & REF #11426346910:, Lot # 24158720; Roche Applied Science) to generate digoxigenin-labeled and fluorescein-labeled riboprobes using a plasmid template containing cDNA ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Ten million cells were then lysed with a Dounce homogenizer on ice in the kit lysis buffer supplemented with EDTA-free protease inhibitors (Roche). Cell homogenates were subjected to immunoprecipitation using magnetic beads-coupled anti-TOMM22 antibodies ...
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bioRxiv - Plant Biology 2023Quote: The YFP digoxygenin-labelled antisense and sense RNA probes were generated by in vitro transcription according to the instructions provided with the DIG RNA labelling kit (SP6/T7; Roche) using a plasmid containing the YFP sequence as template ...
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bioRxiv - Plant Biology 2023Quote: ... We used the SYBR Premix Ex Taq II reagent kit (Vazyme, Q311-02) to perform qRT-PCR on a LightCycler 96 Real-Time PCR System (Roche). Rice OsACTIN1 was used as the internal reference ...
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bioRxiv - Cancer Biology 2024Quote: ... The size and concentration of each sample’s library were determined using Agilent 2200 Tapestation and KAPA library quantification kit (Roche, #KK4824) respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression was detected using the LightCycler 480 SYBR Green I Master Mix or Probes Master Mix kit (Roche Diagnostics) after PCR (5 min at 95 °C ...
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bioRxiv - Developmental Biology 2024Quote: RNA-seq libraries were constructed by the ICM Platform (PARIS) from 100 ng of total RNA using the KAPA mRNA Hyperprep kit (Roche) that allows to obtain stranded polyA libraries ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was carried out using the KAPA HiFi Hot Start Ready Mix PCR Kit (Cat# KK2602, KAPA Biosystems, Wilmington, MA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Antisense and sense RNA probes were synthesized from the linearized plasmid template by in vitro transcription using a DIG RNA Labeling Kit (Roche). In situ hybridization was performed on cryo-sections of telencephalon as previously described (Masai et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were base-pair selected based on Agilent 2100 Bioanalyzer profiles and normalized determined by KAPA Library Quantification Kit (Roche). The libraries were built from 3 to 5 different mice per genotype ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA (150-200 ng) was sheared and HiSeq sequencing libraries were prepared using the KAPA Hyper Prep Kit (Roche). Libraries were amplified by PCR (10 cycles) ...
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bioRxiv - Microbiology 2022Quote: A fraction of each RNA sample analyzed by RNAseq was reverse-transcribed to cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche) and random hexamer primers ...
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bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2022Quote: ... In all available cases staining was performed on the Benchmark ultra-automated Stainer (Ventana) using diamino-benzidine as chromogen (DAB detection kit; Ventana, Roche), and anti-AGR2 monoclonal antibody (1:100 ...
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bioRxiv - Genomics 2022Quote: ... Pooled library (18 total – nine from DNA and 9 from total RNA – see below) concentration was determined using a KAPA library quantification kit (Roche). Libraries were sequenced on a Illumina NextSeq 500 instrument (2×150bp paired-end reads ...
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bioRxiv - Microbiology 2022Quote: ... [30] The RNA was then extracted with the MagNA Pure 96 DNA and Viral NA Small volume kit (06 543 588 001, Roche) on the MagNA Pure 96 Instrument (06541089001 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-10 5 micron sections were extracted using the High Pure FFPE RNA Isolation kit (Roche Life Sciences, Penzberg, Germany) under RNase free conditions following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting RNA was used as input into library preparation at the first strand synthesis step using the KAPA RNA Hyper Prep Kit (Roche). Libraries were sequenced to a depth of 20 million paired-end 2×150bp reads each on the NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and visualized with anti-DIG-AP antibody according to the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) instructions on a Chemidoc MP.
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bioRxiv - Cancer Biology 2022Quote: ... followed by DNA purification and amplification for the library with index primers using KAPA HiFi HotStart Kit (Roche, Indianapolis, IN). The libraries were purified ...
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bioRxiv - Cell Biology 2022Quote: ... RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche, Wilmington, MA) using 250 ng of total RNAs as input ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (Biolaps) according to the manufacturer protocol on a LightCycler real-time PCR thermocycler (Roche). The analysis of the infection rate based on PCR data was done with the binGroup package [42] in R software.
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bioRxiv - Microbiology 2024Quote: ... Plate-based DNA library preparation for Illumina sequencing was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Kapa Biosystems library preparation kit (Roche). 200 ng of sample DNA was sheared to 300 bp or 600 bp using a Covaris LE220 focused-ultrasonicator ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorophores with the least autofluorescence on FFPE tissue were selected to minimize false positives: Cyanine 5 (Cy5) (DISCOVERY CY5 Kit, Cat#760238, Roche Diagnostics Corporation ...
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bioRxiv - Cancer Biology 2024Quote: The Terminal dUTP Nick End Labeling (TUNEL) and In Situ Cell Death Detection Kits (TMR Red #12156792910; Roche Applied Science) were used to examine apoptosis ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA (1 μg) was reverse transcribed to cDNA using the Transcriptor First Strand cDNA Synthesis Kit (catalog no. 04379012001, Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1μg of total RNA was reverse transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Penzberg, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... as in viral infection.9 TFIIB knockdown libraries were prepped with KAPA Stranded RNA-seq Kit with RiboErase (HMR) (Roche) using 500 ng of input RNA and amplified for 9 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibody labelling (16-120 min) and signal detection carried out with the OptiView DAB IHC Detection Kit (VENTANA/Roche), as described previously (33) ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 500 ng of RNA were then retrotranscribed using the Transcriptor First Strand cDNA Synthesis kit (Roche LifeScience) as previously described (Montañés et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared using 500 ng of total RNA by using KAPA stranded RNA-seq kit for Illumina (Roche) as per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... To detect for DNA containing complementary sequences on membrane-bound DNA a α-32P-dCTP-labelled probe spanning the region of 37-611 nts on human mtDNA was synthesized using High Prime DNA Labeling Kit (Roche). After pre-hybridizing the membrane with Church’s buffer (250 mM NaPi pH 7.2 ...
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bioRxiv - Microbiology 2024Quote: ... and 100 ng were used for paired-end Illumina sequencing library preparation using the KAPA HyperPlus Library Preparation Kit (Roche) and the KAPA Unique Dual-Indexed Adapater Kit (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic PCR for ATP13A2 floxed or KO alleles was performed using 100 ng genomic DNA and the Kapa2g Fast HotStart PCR Kit (Roche). PCR primers included a forward primer (5’-CTGCAGCTTCGAGAGGAAAG-3’) ...