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Citations for Roche :
4251 - 4300 of 5184 citations for Lipoteichoic Acid LTA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
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bioRxiv - Cancer Biology 2021Quote: Reverse transcription for qPCR analysis was done with the first strand synthesis kit (Fermentas) using oligo (dT)18 as a primer and qPCR was carried out with LightCycler 96 (Roche) using FastStart Essential DNA Green Master Mix (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: The in vitro synthesis of digoxigenin (DIG)-labeled antisense SatIII probe (158 bp SatIII repeat cloned in 1μg of PGEM-2-98 construct) with T7 RNA polymerase was carried out using the DIG labeling kit from Roche Products Pvt ...
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bioRxiv - Cancer Biology 2021Quote: ... libraries were prepared in an automated fashion using an Agilent Bravo robot with aKAPA Standard mRNA-Seq Kit (KAPA BIOSYSTEMS). In-house sequencing adaptors were ligated to 100-300 bp fragments of input dsDNA ...
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bioRxiv - Genomics 2019Quote: ... Genome libraries were prepared using the KAPA Hyper Library Preparation Kit with Standard PCR Library Amplification (Kapa Biosystems, Wilmington, MA) and sequenced on an Illumina MiSeq using V3 sequencing chemistry (Illumina Inc. ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using the LightCycler 480 Probe Master Kit and the LightCycler 480 II system (Roche Applied Science) as described previously92 ...
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bioRxiv - Microbiology 2021Quote: ... Radiolabeled probe was generated by purifying a digested (Ncol; 1 cut) pCpG vector DNA using High Pure PCR product Purification Kit (Roche) followed by radiolabeling the probe using Random Prime DNA labeling kit (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Illumina compatible Indexed libraries were prepared from 12ng of Diagenode Biorupter Pico sheared ChIP DNA using the KAPA Hyper Library Preparation Kit (Roche). Libraries were enriched with 2 cycles of PCR then assessed for size distribution using the 4200 TapeStation High Sensitivity D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... 1μl of cDNA diluted with 10-fold MQ was mixed with KAPA SYBR Fast qPCR kit and amplified with Lightcycler 96 (Roche; 95℃ 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Triplicate reactions that amplified were pooled together and quantified with Kapa library quantification kit (Kapa Biosystems, KK4824, Wilmington, MA, USA) before equimolar sample mixing ...
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bioRxiv - Immunology 2020Quote: ... TUNEL assays were performed on sectioned tissue using the TMR red In Situ Cell Death Detection Kit (Roche, Basel, Switzerland), following the manufacturer protocol for labeling of cryopreserved tissues ...
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bioRxiv - Immunology 2020Quote: ... The libraries were quantified using the KAPA SYBR FAST ABI Prism Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA). Equimolar amounts of each library were sequenced on a NextSeq 500 instrument controlled by the NextSeq Control Software (NCS ...
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bioRxiv - Immunology 2020Quote: ... Amplifications were carried out on a LightCycler 480 II instrument using LightCycler 480 software (v. 1.5.0) and a LightCycler 480 SYBR green I master kit (Roche Applied Science) according to the provided instructions ...
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bioRxiv - Immunology 2021Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Immunology 2021Quote: ... cell nuclei with DNA strand breaks at the early stage of apoptosis were labeled using fluorescein-dUTP in the TUNEL reaction (In Situ Cell Death Detection Kit; Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... finally hybridized for 3h at 42 °C with DIG-labelled telomere probes and bulk telomere length analysed as recommended by the manufacturer (Telo-TAGGG Telomere Length Kit Assay; Roche). For TRF on APC vesicle preparations ...
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bioRxiv - Immunology 2021Quote: ... Poly A mRNA was purified from total RNA using oligodT magnetic beads and strand-specific indexed libraries were prepared using the Illumina’s TruSeq Stranded mRNA Sample Prep Kit followed by ten cycles of amplification using KAPA HiFi DNA polymerase (KAPA Biosystems). Libraries were quantified and pooled based on a post-PCR Bioanalyzer and 75 base pair (bp ...
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bioRxiv - Immunology 2021Quote: ... Synthesized DNA specific to regions of homologous recombination (see Oligonuleotide section in Key Resources Table) was labeled with 32P (Amersham) using the High Prime labeling kit (Roche) according to manufacturer’s instructions and unincorporated 32P was removed with G-50 Sephadex Quick Spin columns (Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Telomere dot blot was performed by 3h hybridization at 42°C with DIG-labelled telomere probe as recommended by the manufacturer (Telo-TAGGG Telomere Length Kit Assay; Roche). Control Input was 200 ng genomic DNA from APCs ...
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bioRxiv - Immunology 2021Quote: Sample RNA extraction was performed from freshly isolated C57BL/6J WT pMBEMCs following the manufacturers instruction of the High Pure RNA Isolation Kit (Hoffman-La Roche). cDNA was obtained from total isolated RNA of each sample with the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... RNA mRNA libraries of template molecules suitable for high throughput DNA sequencing were created using the KAPA mRNA HyperPrep Kit (Roche). Briefly ...
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bioRxiv - Genomics 2020Quote: ... Quantification and quality control of the final HiChIP library was conducted using a Bioanalyzer and KAPA quantification kit (Kapa Biosystems). Libraries underwent Next Generation Sequencing on a HiSeq 2500 generating 100 bp paired-ends.
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bioRxiv - Neuroscience 2021Quote: ... Alkaline hydrolysis was then performed at 60°C for 30 min to fragment the target RNA before labeling it with Digoxigenin and Fluorescein according to kit instructions (Roche).
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bioRxiv - Microbiology 2021Quote: ... Library concentration for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Immunology 2022Quote: ... and 11-week infected half brains and liver lobes of mice (n=4/group) was extracted and purified using a High Pure PCR Template Prep Kit (Roche). DNA concentration of each sample was determined via NanoDrop ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA was enriched by poly-A capturing and RNA-seq libraries were generated using KAPA mRNA HyperPrep Kit (KAPA Biosystems).Libraries were sequenced using the Illumina HiSeq 1500 platform.
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bioRxiv - Cancer Biology 2022Quote: ... Genomic DNA contamination was removed by DNase I treatment of the isolated RNA using DNase I RNase free kit (Roche). After quantification of the RNA and quality check ...
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bioRxiv - Cell Biology 2022Quote: Following library construction as per manufacturer’s instructions ST libraries were quantified using the KAPA-Illumina PCR quantification kit (KAPA Biosystems) and pooled at 4nM concentration with a sample ratio corresponding to the surface area of tissue coverage obtained from the H&E imaging ...
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bioRxiv - Neuroscience 2022Quote: cDNA was generated from TRAP-isolated and total input RNA samples (n=2 samples/group) using the Transcriptor First Strand cDNA Synthesis Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by using 1:500 diluted rabbit polyclonal antibodies against ferroportin (49) and an appropriate detection kit (Omnimap rabbit polyclonal HRP, #760-4311 and ChromoMap-DAB #760-159; Roche). Negative controls were performed by the omission of the primary antibody ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted from the tail tip using the KAPA Express Extract DNA Extraction Kit (Kapa Biosystems, London, UK) and Animal Tissue Direct PCR Amplification Kit (with TL ...
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bioRxiv - Molecular Biology 2022Quote: ... The blots were hybridized with the MVC VP2 genome probe using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Probe labelling and DNA hybridization were performed following the protocol provided with the PCR-DIG DNA-labelling and chemiluminescent detection kit (Roche).
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bioRxiv - Cell Biology 2022Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using the In Situ Cell Death Detection Kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: 100 to 500 ng of total RNAs were reverse-transcribed with Transcriptor First Strand cDNA synthesis kit (Roche, Basal, Switzerland). qPCR experiments were performed using the StepOnePlus qPCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... according to the manufacturer’s instruction and reverse-transcribed with the aid of Transcriptor First Strand cDNA synthesis kit (Roche, Switzerland). qPCR experiments were performed using the StepOnePlus qPCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche, Wilmington, MA) using 250 ng of total RNAs as input ...
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bioRxiv - Cancer Biology 2022Quote: ... Validation of the library was performed on Fragment Analyzer (Standard Sensitivity NGS kit) and using qPCR (ROCHE Light Cycler480, France). Total RNAs were sequenced on NovaSeq 6000 from Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Lactate dehydrogenase (LDH) assay kit (LDH Cytotoxicity Detection KitPLUS) were obtained from Roche (Germany). MG-63 cell line (National Cell Bank of Iran (NCBI) ...
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bioRxiv - Cancer Biology 2022Quote: PC3 cell proliferation was quantified by an MTT based colorimetric assay using the Cell Proliferation Kit I (Roche, Sigma Aldrich). WT ...
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bioRxiv - Developmental Biology 2022Quote: ... Digoxigenin (DIG)-labeled RNA probes were transcribed in vitro using a DIG RNA labeling kit (Roche Applied Science, Basel, Switzerland) and brain sections were hybridized with gene-specific antisense RNA probes ...
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bioRxiv - Cancer Biology 2022Quote: ... and 500 ng of DNA-free RNA was reverse transcribed using the First Strand cDNA Transcriptor Synthesis Kit (Roche Life Science, Roche Diagnostics Division ...
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bioRxiv - Cancer Biology 2022Quote: ... the DNA fragments were “End-Repaired,” “A-tailed,” and the index adapters were ligated (Agencourt AMPure XP Beads; SeqCap EZ Pure Capture Bead Kit; Hoffmann-La Roche). Following that ...
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bioRxiv - Developmental Biology 2022Quote: 155BS-upd from Doug Harrison and RE35237 from BDGP cDNAs were used to generate upd and sna RNA probes using the DIG RNA Labeling Kit (Roche). Secondary biotinylated antibody against mouse (1:200 ...
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bioRxiv - Genomics 2022Quote: ... RNA Libraries were prepared from 100 ng of total RNA with the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems KK8401). Indexed libraries were then pooled and 2×50 bp ...
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bioRxiv - Genomics 2022Quote: ... and P5 and P7 adapters were ligated per pool following the KAPA HiFi Hotstart PCR kit standard protocol (Kapa Biosystems). Pooled samples were then combined into one sample and cleaned as before ...
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bioRxiv - Plant Biology 2022Quote: ... Sense and anti-sense digoxigenin-labelled probes were synthesised using the DIG RNA Labeling Kit (SP6/T7; Roche, Basel, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... the ligated product was amplified by 12 PCR cycles using the Kapa Hifi Hotstart NGS library amplification kit (Kapa Biosystems), followed by purification with 0.6×AMPure XP ...
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bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
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bioRxiv - Plant Biology 2022Quote: ... The RNA level was detected using the DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche, Cat. 11585614910).