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Citations for Roche :
4151 - 4200 of 5184 citations for Lipoteichoic Acid LTA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... Library quality was assessed using an Advanced Analytical Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Roche). Sequencing has been performed on a NovaSeq6000 S4 lane (Illumina ...
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche). 1:5 diluted cDNAs was used for qPCR by using KAPA SYBR® FAST qPCR Master Mix (5X ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with RCAS-hPDGFB-HA and RCAS-shRNA-p53-Rfp using a Fugene 6 transfection kit (Roche, 11814443001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability analysis by lactate dehydrogenase (LDH) release assay was performed with the Cytotoxicity Detection Kit (LDH) (Roche, Mannheim, Germany) and has also been described 23,31.
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bioRxiv - Microbiology 2020Quote: ... Library concentrations for pooling of barcoded samples was assessed by RT-qPCR with a KAPA Library Quantification kit (KAPA Biosystems) as recommended for high sensitivity ...
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bioRxiv - Genomics 2021Quote: ... using 400 ng of total RNA from each sample for poly(A) mRNA capture followed by stranded mRNA-seq library construction using KAPA stranded mRNA-seq kit for Illumina (Roche) as per manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... Final libraries with different sample indices were pooled in equimolar ratios based on quantification using KAPA library quantification kit for Illumina platforms (Roche) and size analysis on Fragment Analyzer (AATI ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons were purified with 0.8x AMPure XP beads and 100ng of DNA was converted to paired-end Illumina sequencing libraries using the KAPA HyperPlus library preparation kit (Roche) with the KAPA unique dual-indexed adapters (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR optimisation was carried out on the full-length cDNA using the KAPA HiFi PCR kit (Kapa Biosystems, Boston USA) and 12 cycles was sufficient to generate the material required for SMRTbell library preparation ...
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bioRxiv - Microbiology 2019Quote: One-step simplex real-time quantitative RT-PCR amplifications were performed using Multiplex RNA Virus Master Kit (Roche Diagnostics, France) for influenza A ...
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bioRxiv - Plant Biology 2021Quote: ... Relative transcript abundancies were determined using the Roche LightCycler 480 and the LC480 SYBR Green I Master Kit (Roche Diagnostics). The resulting cycle threshold values were converted into relative expression values using the second derivative maximum method and ACTIN2 ...
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bioRxiv - Molecular Biology 2020Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Plant Biology 2021Quote: ... 50 ng of DNA was subjected to PCR amplification using the Kapa HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, USA). The individual PCR reactions were performed in 20 μL final volume and contained:
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bioRxiv - Microbiology 2021Quote: ... Between 150 and 200 ng of genomic DNA was sheared acoustically and HiSeq sequencing libraries were prepared using the KAPA Hyper Prep Kit (Roche). PCR amplification of the libraries was carried out for 10 cycles ...
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bioRxiv - Neuroscience 2021Quote: ... and quantitative real-time PCR was performed using the LightCycler DNA Master SYBR Green I Kit in a LightCycler 480 System (Roche). Oligonucleotides used for quantitative real-time OCR are listed in supplemental table S2 ...
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bioRxiv - Immunology 2021Quote: ... 25-100 ng of total RNA was used to deplete rRNA using DNA probes and RNase H provided in RiboErase (HMR) kit (Roche). The depleted RNA was fragmented prior to cDNA synthesis followed by Illumina adaptor ligation using KAPA RNA HyperPrep kit (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was hybridized with digoxigenin-labeled DNA probes synthesized with a PCR DIG probe synthesis kit (Roche Molecular Biochemicals) as recommended by the supplier ...
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bioRxiv - Cancer Biology 2021Quote: ... Stranded RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) and KAPA Unique Dual-Indexed Adapters (Roche) according to instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and 100 ng of DNA was converted into paired-end Illumina sequencing libraries using KAPA HyperPlus library preparation kit (Roche) with the KAPA unique dual-indexed adapters (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNAseq libraries were prepared from 1 μg of total RNA per sample using the KAPA Stranded mRNA Sequencing Kit (Roche). In short ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplification was performed with the LC FastStart DNA master SYBR green I kit in a LightCycler 1.2 instrument (Roche Diagnostic) using the following specific primers ...
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bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
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bioRxiv - Microbiology 2021Quote: ... and used to generate the sequencing libraries using the KAPA Stranded mRNA Seq kit for the Illumina sequencing platform according to the manufacturer’s protocol (KAPA biosystems). Libraries were sequenced on an Illumina HiSeq 2500 in the Rapid Run mode using 100 nt single read sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
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bioRxiv - Neuroscience 2022Quote: ... to estimate the quantity and validate the size distribution and were then quantified by qPCR using the KAPA Library Quantification Kit KK4835 (# 07960204001, Roche) prior to the amplification with Illumina’s cBot ...
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bioRxiv - Cancer Biology 2022Quote: ... 500-1000 ng of sheared DNA was used as a template for a 6-cycle PCR to construct a fragmented library using the KAPA HTP Library Preparation Kit (Roche). Exome enrichment was performed using SeqCap EZ Enrichment Kit (Roche ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The sheared fragments were treated with end repair and A-tailing using the KAPA-Illumina library creation kit (KAPA biosystems) followed by immobilization of mate pair fragments on strepavidin beads (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was performed using an Eva Green 2X qPCR MasterMix-No Dye kit (Abm, MasterMix-S) on a LightCycler 480 instrument (Roche). Relative gene expression was analyzed based on the 2-ΔΔCt method with β-actin as internal controls ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with the LightCycler 480 SYBR Green I Master kit (Cat. 04887352001, Roche). Primers for pmp-3 and ACTIN were used as housekeeping for C ...
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bioRxiv - Cancer Biology 2022Quote: Viability and cell growth ratios were determined analyzing cell density by MTT using the Cell Proliferation Kit I (Roche, 11465007001) in DND41 cells treated with EX-527 (10-6 to 10-3 M ...
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bioRxiv - Cell Biology 2022Quote: ... the RNA concentration was determined and 1 µg RNA was used for the cDNA synthesis according to the Transcriptor First Strand cDNA Synthesis kit (Roche). The expression of the following genes FTSH7 (AT3G47060) ...
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bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s protocol. Quantitative PCR was performed using SensiMixTM II Probe Kit (Cat#. BIO-83005) with Universal ProbeLibrary (Roche Diagnostics), and the CFX96 Touch System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... The KAPA Stranded mRNA Sample Preparation Kit was used to prepare polyA enriched RNA libraries (Kapa Biosystems, Wilmington, MA, USA). Trimmomatic [73] (version 0.36 ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR products were then purified and reverse transcribed with digoxigenin labeling using a DIG RNA labelling kit (Roche, Switzerland). For dissected adult pituitary or 5-8 dpf larvae ...
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bioRxiv - Genomics 2019Quote: ... The dry contents were resuspended by addition of 7.5 µl hybridization buffer and 3 µl hybridization component A (SeqCap EZ Hybridization and Wash Kit: 05 634 261 001, Roche), mixed by tapping ...
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bioRxiv - Genomics 2019Quote: ... per sample were washed twice with 200 µl 1x wash buffer (SeqCap EZ Hybridization and Wash Kit: 05 634 261 001, Roche), once with 100 µl ...
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bioRxiv - Genomics 2019Quote: ... Non-target DNA was removed through washing the beads with buffers of the SeqCap EZ Hybridization and Wash Kit (05 634 261 001, Roche) as per manufacturers instructions with the following modifications ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Libraries were subsequently index-amplified for 20 cycles using a KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, Ma. USA) in 50-μl reactions following the manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2019Quote: We prepared sequencing libraries for the ten de novo sequenced isolates using the Kapa Biosystems kit (Kapa Biosystems, Woburn, MA) and ~1μg of DNA of each isolate according to manufacturer’s protocols ...
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bioRxiv - Genetics 2019Quote: ... 1 ug purified digested plasmid was used as template for SP6 or T7 transcription using the DIG-RNA labeling kit (Roche). Following probe transcription ...
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bioRxiv - Biochemistry 2019Quote: ... RNA-seq libraries were prepared using the KAPA Stranded mRNA-Seq Kit (KAPA Biosystems, Wilmington, MA, USA, Cat. No. KK8421) from 250 ng of total RNA ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescent probes with BAC DNAs were prepared by nick translation with dATP conjugated with fluorophore or DIG using Nick Translation kit (Roche) or PCR ...
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bioRxiv - Genomics 2019Quote: ... followed by quantitation using real-time PCR using the KAPA Library Quant Kit for Illumina (KAPA Biosystems catalog no. KK4835). Libraries were then pooled and sequenced on the Illumina NextSeq 500 platform using Illumina sequencing reagents according to manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: ... Amplified genomic DNA (70 ng) was used to generate exome sequence libraries using the Hyper Prep kit (Kapa Biosystems, KK8504), SureSelect Target Enrichment kit (Agilent Technologies ...
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bioRxiv - Genetics 2019Quote: ... located in exon 18 and reverse primer 5’-TTGGTGGCTACAAAGACGTG-3’ located in exon 22 using the One Tube reverse transcription-PCR reaction kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR analysis of cytokine related porcine gene expression was performed using the KAPA SYBR-FAST qPCR Kit (KAPA Biosystems), using the primers shown in Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... and viability was measured using the Roche MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) kit (Roche, Cat # 11465007001) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: Total DNA was isolated from L3 larvae or 5-days old adults of Iduna homozygous mutants and control sequencing strain using the Roche genomic DNA extraction kit (Roche). To confirm the mutant line ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were hybridized with digoxigenin-labeled antisense GUARDIN probes synthesized using T7 RNA polymerase using the DIG Northern Starter Kit (Roche).