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Citations for Roche :
4201 - 4250 of 4283 citations for Mouse Anti Dengue Virus Serotype 2 NS1 Antibody CM474 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the Dig at the end of the DNA-bead conjugate was first blocked with anti-digoxigenin Fab fragment (Roche) in a ratio of 1:2 respectively in Buffer C with 7 mM MgCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with horseradish peroxidase (HRP)-conjugated anti-DIG (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010 ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted proteins were separated on 8% SDS-PAGE gel (37.5:1 acrylamide:bis-acrylamide) and probed with anti-HA (Roche, 3F10), anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... positive cells in the human biopsies but with the visualization systems (Omni-Map anti-Rabbit) conjugated to horseradish peroxidase developing the reactions with the 3,3’-diaminobenzidine tetrahydrochloride (ChromoMap DAB, Ventana, Roche) and purple chromogen (Discovery Purple Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described (Pearson et al., 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910 ...
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bioRxiv - Molecular Biology 2021Quote: ... lysed with immuno-precipitation (IP)-lysis buffer (from IP Lysis kit Pierce) and supplemented with anti-protease (Roche, Basel, Switzerland). Co-immuno-precipitation (Co-IP ...
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bioRxiv - Cancer Biology 2019Quote: RNA sense and anti-sense probes were synthesized from pcsDest2-gdf6a and pcsDest2-gdf6b constructs using DIG RNA Labeling Kit (Roche) per the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of immunocomplexes was assessed by probing protein gel blots with either anti-HA (3F10, Sigma/Roche; http://www.sigmaaldrich.com/) or anti-Myc antibody (9E10 ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Probed embryos were blocked for 1-2 hours at ambient temperature in 5% Goat sera in PBST before detection overnight (∼16 hours) at 4°C using an anti-Digoxigenin-Fab fragments conjugated to Alkaline Phosphatase enzymes (1:1000 dilution, Roche) in 5% Goat Sera in PBST ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the detection of the Dig-labelled riboprobe with an anti-DIG Fab fragment conjugated with alkaline phosphatase (1:750; Roche). The colometric reaction was performed using mixture of 5-Bromo-4-chloro-3-indolyl phosphate (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ml of the lysates prepared with IP extracation buffer from 0.5 g of leaves was incubated with anti-HA Affinity Matrix (Roche 11815016) for o/n in a rotary shaker at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Purified DNA amplicons with flanking T7 and SP6 promoters were used for in vitro synthesis of digoxigenin (DIG)-UTP-labelled single-stranded RNA sense and anti-sense probes by using T7 and SP6 polymerases from a DIG RNA Labelling Kit (Roche). The non-complementary sense probe was used as negative control for each gene ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal amounts (Protein estimated by Bradford) of soluble fractions were incubated with 40 μl of pre-equilibrated anti-HA affinity agarose gel (Roche) in binding buffer (Lysis buffer) ...
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bioRxiv - Molecular Biology 2019Quote: ... Probe labelling and detection of the blot were carried out using the DIG-DNA labelling mix and detection reagents (Anti-Digoxigenin-AP) (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... TNbuffer 10 min at RT and TNT buffer 15’ at RT) and by the incubation of cryo-section using anti-digoxigenin-ap fab fragments (1/100, #11093274910, Roche) dissolved in TNbuffer for 2 hours at RT ...
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bioRxiv - Biophysics 2021Quote: ... or a multiply digoxigenin-labeled tail (MT) to the coverslip coated with anti-digoxigenin (Roche Life Science, Indianapolis, IN, USA). The opposite end of the DNA was attached to a streptavidin-coated bead via a single biotin (TPM) ...
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bioRxiv - Developmental Biology 2022Quote: ... ISH was performed as described previously (Kraus et al., 2016) with minor changes in the RNA probe detection step: anti-Digoxigenin-AP Fab fragments (Roche) diluted 1:4000 in 0.5% blocking reagent (Roche ...
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bioRxiv - Genetics 2022Quote: ... Samples were blocked for 30 minutes in a solution of 1% BSA in 2x SSC with 0.1% tween at room temperature and then rhodamine-conjugated anti-digoxigenin (Roche, #11207750910) or FITC-conjugated anti-biotin (AbCam ...
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bioRxiv - Biophysics 2022Quote: ... except that the carboxylic silica beads were replaced by polystyrene carboxylic beads and polyclonal anti-digoxigenin from sheep (11333089001, ROCHE) were used in our experiments.
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bioRxiv - Plant Biology 2019Quote: ... Expression of prey fusion proteins was confirmed by immunoblotting with anti-HA-HRP conjugate (clone 3F10, Roche, Indianapolis, IN, USA). Proteins were detected using chemiluminescent Pierce ECL 2 substrate (Thermo Fisher Scientific ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... anti-PD-L1 (10 mg/kg for the first dose with each subsequent dose at 5 mg/kg; atezolizumab, Roche), anti-TGF-β (10 mg/kg ...
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bioRxiv - Immunology 2020Quote: ... To quantify NETs we measured HNE-DNA complexes using ELISA specific for HNE and anti-DNA Ab of Cell Death Detection ELISAPLUS (Roche). Briefly ...
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bioRxiv - Synthetic Biology 2021Quote: ... the efficiency of biotinylation was checked in a Western blot revealed with POD-anti-biotin (horseradish peroxidase conjugated with Streptavidin, Roche).
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bioRxiv - Biophysics 2019Quote: ... The central fragment of the molecule is flanked by oligonucleotides labelled either with digoxigenin (3’ end) or biotin (5’ end) that specifically bind either to a glass surface covered with Anti-digoxigenin (Roche) or to superparamagnetic beads (MyOne ...
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bioRxiv - Cell Biology 2020Quote: ... anti-SPD-5 (1:1000, a generous gift from B. Bowerman, Hamill et al., 2002) and anti-GFP (1:500, Roche) overnight at 4°C and with secondary antibodies Alexa488 (1:500 ...
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bioRxiv - Developmental Biology 2020Quote: Colorimetric in situ hybridizations were performed as described in (Pearson et al., 2009) using the anti-DIG-AP (Roche 11093274910) at 1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.78 g/L CSM-His and 20 g/L agar) and screened for expression of Erg6-GFP by Western blot with anti-GFP bodies (11814460001; Roche) and for localization of Erg6-GFP to LDs by fluorescence microscopy.
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bioRxiv - Cell Biology 2020Quote: ... and SD-N (0 and 24 h) samples were TCA precipitated [21] and analyzed by Western blot with anti-GFP bodies (11814460001; Roche). All the experiments were done at least in duplicate.
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bioRxiv - Developmental Biology 2021Quote: ... brn3a1 and brn3a2(l) transcripts were visualized with anti digoxigenin-alkaline phosphatase conjugates and BCIP/NBT or BM purple substrates (Roche). For two color WISH krx-20 (Oxytoby and Jowett ...
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bioRxiv - Molecular Biology 2022Quote: ... The inner bottom surface of the flow cell is coated with an hydrophobic solution (nitrocellulose or Sigmacote) to allow attachement of anti-Digoxigenin (11093274910, Roche) Lionnet et al ...
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bioRxiv - Plant Biology 2022Quote: ... Sense and anti-sense digoxigenin-labelled probes were synthesised using the DIG RNA Labeling Kit (SP6/T7; Roche, Basel, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Whole mount larvae and adult brain sections layered on glass slides were blocked for at least one hour in PBT with 2 mg/ml bovine serum albumin and 2% sheep serum at room temperature and then incubated overnight at 4°C with alkaline phosphatase-coupled anti-DIG antiserum (11093274910, Roche) diluted 1/5000 in blocking solution ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products with flanking T7 promoters were used as template for the synthesis of anti-sense riboprobes using a T7 RNA polymerase (Roche) (Mercurio et al ...
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bioRxiv - Developmental Biology 2023Quote: ... Resulting amplicons were used as templates for the synthesis of either digoxigenin (DIG) or fluorescein (FITC)-labelled anti-sense probes by in vitro transcription using T7 or T3 polymerases (Roche) and DIG or FITC RNA labelling mixes (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the protein A fragment of the TAP tag or anti-HA peroxidase 1/500 (clone 3F10, Roche).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in PBS for 30 min and incubated overnight at 4°C with 1:1,500 anti-HA high affinity (Roche, 3F10). After three PBS washes ...
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bioRxiv - Developmental Biology 2023Quote: ... Purified plasmids were used to prepare digoxigenin-labelled sense and anti-sense riboprobes using T7 and SP6 RNA polymerases (Roche). Probes were purified using ethanol precipitation and resuspended in 1:1 volume of DEPC-treated water:formamide.
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bioRxiv - Microbiology 2023Quote: ... Lysates were centrifuged at 10,000 x g for 15 minutes to pellet and remove insoluble material after which the remaining soluble lysate was incubated with anti-HA sepharose beads (Roche) for 4 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C), the lysate (IN, input) was transferred to either 50 µl of HA bead slurry (anti-HA affinity matrix, Roche) or 50 µl c-myc bead slurry (EZview red anti-c-myc affinity gel ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were lysed in equilibration buffer (20 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8) supplemented with lysozyme and anti-protease cocktail (Roche), followed by sonication ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were blocked for 30 minutes in 1% Roche Western Blocking Reagent prior to incubating overnight at 4°C with either anti-FITC with horseradish peroxidase conjugate (Roche) at a 1:2000 concentration or anti-DIG-with horseradish peroxidase conjugate (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were hybridized with digoxigenin (DIG)-labeled riboprobes overnight at 65°C and incubated overnight with 1/2000 alkaline phosphatase-conjugated anti-DIG Fab fragment (Roche) at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The larvae were then incubated overnight at 4°C with anti-Dig-AP (1:2000 in 5% normal goat serum, 11093274910, Roche) and washed before detecting alkaline phosphatase using NBP/BCIP(Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed 6 times with MABT and were then incubated with anti-DIG AP-conjugated Fab fragment (Roche, 11093274910), Alexa-Fluor 488 goat anti-Mouse IgG1 antibody (ThermoFisher A-21121 ...
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bioRxiv - Neuroscience 2024Quote: ... antibody in-situ stainings were done as described previously (Haussmann et al., 2008) for validation using rat anti-HA (MAb 3F10, 1:20; Roche), rabbit anti-GFP (Molecular Probes ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were blocked and then incubated with 1/4000 concentration of anti-DIG Fab fragment conjugated with alkaline phosphatase (Roche) overnight at 4ºC ...
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bioRxiv - Developmental Biology 2021Quote: ... Colorimetric in situ hybridization assays used sense and anti-sense RNA probes labeled with DIG RNA labeling mix (Roche, Basel, Switzerland), following a published procedure (Sharma et al. ...
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bioRxiv - Immunology 2019Quote: ... the wells were washed five times and incubated with 100 µl/well goat anti-human IgG conjugated with alkaline phosphatase (Roche Diagnostics) diluted 1 in 5,000 for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were re-probed to detect ZMPSTE24 and Rce1 using rat anti-HA (clone 3F10, Roche cat #11867423001; 1:10000 dilution), and polyclonal rabbit anti-Rce1 [52] (1:2000 dilution) ...
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bioRxiv - Neuroscience 2021Quote: ... for 2 hours at room temperature and left to incubate for 24 hours with blocking buffer containing a 1:200 dilution of anti-DIG (Roche, 11207733910) or anti-fluorescein antibody conjugated with POD (Roche ...