Labshake search
Citations for Roche :
4101 - 4150 of 5389 citations for QuantiChrom Magnesium Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed following citrate buffer antigen retrieval using an in situ cell death detection kit (Roche, 11684795910). Positive control slide was treated with deoxyribonuclease to induce DNA damage (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... and 500 ng of DNA-free RNA was reverse transcribed using the First Strand cDNA Transcriptor Synthesis Kit (Roche Life Science ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, KK4610). cDNA was synthesized from human cells using SuperPrep II Cell Lysis & RT Kit (TOYOBO ...
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bioRxiv - Immunology 2022Quote: ... Apoptotic cells were detected in dissected guts using the TMR red In Situ Cell Death Detection Kit (Roche; 12156792910) following standard kit staining protocol ...
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bioRxiv - Genomics 2022Quote: A DNA library was prepared from the WGA sample using the Kapa LTP Library Preparation Kit (KAPA Biosystems, Roche7961880001) and sequenced on the Illumina MiSeq platform with the MiSeq Reagent Kit v3 to generate 2×150 bp paired-end reads ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were prepared by the Bauer Core Facility at Harvard University using a KAPA stranded RNA HyperPrep Kit (Roche Sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche) and 1 µl of RNase A/T1 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction were carried out using MagNA Pure LC total Nucleic acid isolation kit on the automated MagNA Pure LC2.0 platform (Roche) from two to five colonies picked up from fresh cultured plates.
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bioRxiv - Microbiology 2022Quote: ... Library preparation was performed using ∼ 1ug of total RNA processed using RiboErase kits to remove ribosomal RNA (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 °C for 8 min ...
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bioRxiv - Microbiology 2022Quote: ... the water phase was used for RNA isolation with High Pure RNA Isolation Kit (Roche Diagnostics GmbH, Mannheim, Germany), yielding >3 μg total RNA per sample ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction was carried out using the MagNA Pure 96 DNA and Viral NA Small Volume Kit (Roche, Diagnostics) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... were produced with DIG-UTP by in vitro transcription using the Sp6 and T7 promoters according to the manufacturer’s protocol (RNA-labeling kit; Roche). The sections were treated as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Antisense and sense single-strand riboprobes were transcribed in vitro using DIG or FITC RNA labeling kit (Roche Diagnostics). cRNA probes for Gad155 and Slc17a756 were used.
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bioRxiv - Neuroscience 2022Quote: ... and the pool was quantified by qRT-PCR using the KAPA Library Quantification Kit - Illumina/Universal (KAPA Biosystems, KK4824) in combination with the Life Technologies Viia 7 real time PCR instrument ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR reactions were performed using the KAPA SYBR FAST qPCR Master Mix (2X) Kit (KAPA Biosystems, Wilmington, USA) on the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... according to the manufacturer’s protocol and were transcribed into cDNA with the 2nd generation 5’/3’ RACE Kit (Roche) in combination with the Expand High Fidelity PCR System (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit; Roche Tissue Diagnostics). For haematoxylin and eosin (H&E ...
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bioRxiv - Physiology 2022Quote: Both ATP release and intracellular ATP from isolated mesenteric arteries were performed using a ATP Bioluminescence HSII kit (Roche) kit as previously described.3
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bioRxiv - Molecular Biology 2022Quote: The psyllid midguts were diagnosed for cell-death or apoptosis using In situ cell-detection kit TMR-red (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... six reverse primers (10 μM) and Robust DNA polymerase from the KAPA2G Robust PCR Kit (Kapa Biosystems, Wilmington, MA). Cycling conditions were ...
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bioRxiv - Plant Biology 2024Quote: ... and the library was quantified by qPCR against a standard curve with the KAPA Library Quantification Kit (Kapa Biosystems). Libraries were sequenced on a NovaSeq6000 Illumina platform in 150PE mode ...
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bioRxiv - Plant Biology 2024Quote: ... 1 μg of the PCR product was used for RNA probe labeling with T7 RNA polymerase as per the DIG Northern Starter Kit’s Instruction Manual (Roche). Post-hybridization washes and immuno-chemiluminescent detection of the bound probe were conducted following the instructions provided in the DIG Northern Starter Kit manual (Roche) ...
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bioRxiv - Plant Biology 2024Quote: We fragmented 700 ng of high-molecular-weight DNA using a Covaris S220 sonicator and a WGS library was generated for both species using the KAPA Hyper Prep kit with a PCR-free protocol according to the manufacturer’s instructions (Roche). We applied final size selection using a 0.7-fold ratio of AMPureXP beads ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μm sections were stained according to instruction of In situ Cell Death Detection Kit (Roche, Basel, Switzerland, #11684817910). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 1 µg of total RNA using the KAPA Stranded mRNA Hyperprep Kit (96rxn) (Roche, KK8581) according to manufacturer’s specifications ...
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bioRxiv - Genetics 2023Quote: ... Sequencing libraries were prepared using 100 ng fragmented DNA with the KAPA HyperPrep Library Prep Kit (Roche, Indianapolis, IN) and NEXTFLEX UDI barcodes (PerkinElmer ...
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bioRxiv - Molecular Biology 2024Quote: ... The preparation of next generation sequencing libraries was done using the KAPA HyperPrep ChIP Library kit (Roche Sequencing solutions) at the molecular biology and functional genomics platform of the Institut de Recherche Clinique de Montréal (IRCM) ...
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bioRxiv - Molecular Biology 2024Quote: ... membrane hybridization and detection were performed using DIG-High Prime DNA Labelling and Detection Starter Kit II (Roche, #11585614910) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Molecular Biology 2024Quote: Analysis of ChIP-purified DNA by quantitative PCR (qPCR) was performed using KAPA SYBR Fast qPCR kit (KAPA Biosystems) and StepOnePlus real-time PCR systems (Life Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche, #11175025910). Probes were then cleaned with MegaClear Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... For library preparation the Kapa LTP Library Preparation Kit for Illumina Platforms was used (Kapa Biosystems, Part Number KK8232). The cDNA was filtered into the End Repair step of the method and the library preparation was followed as per the protocol through to Adapter Ligation ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA prepared using the KAPA Express reagent (a component of the KAPA Mouse Genotyping Kit from Roche, 07961804001) and analyzed for both CRISPR lesions and repeat size as described below.
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bioRxiv - Genetics 2024Quote: ... 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche) with random primer ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated from cells and reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche). Gene expression was quantified using FastStart Roche SybrGreen (Roche) ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR amplification was performed in two technical replicates using KAPA Hifi Hotstart PCR kit (Kapa Biosystems, MA, USA) via a Bio-Rad CFX96 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... We prepared RNA libraries using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adaptors (KAPA Biosystems; Massachusetts, USA), with 200 ng of RNA as input ...
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bioRxiv - Immunology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Molecular Biology 2022Quote: The library of mtDNA was prepared from 500 ng mitochondrial DNA with a KAPA HyperPlus Kit PRC-free (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... two PCR reactions were performed with PCR anchor primer (included in the 2nd Generation 5’/3’ RACE Kit, Roche) and GB3 (oligo 61 ...
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bioRxiv - Genomics 2023Quote: TP pre-capture libraries were prepared as previously described [15] using the KAPA HyperPlus kit with UDI adapters (Roche). 17.5µL DNA was used as input for fragmentation for eight minutes ...
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bioRxiv - Microbiology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... B-16 cells were collected after 48 hours of transfection and RNA was extracted using (Roche, RNA isolation Kit). Total RNA was quantified on a nanodrop (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription (IVT) was performed using T7 mMassage mMachine kit (invitrogen) and purified using RNA purification column (Roche). MCU-IVT was injected at single cell stage and embryos were cultured in E3 water ...
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bioRxiv - Immunology 2023Quote: ... Poly(A)-enriched next-generation sequencing library construction was performed using the KAPA mRNA Hyper Prep Kit (KAPA Biosystems) with 100 ng of input total RNA and 15 amplification cycles according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 6ng of ChIP DNA was processed for library preparation for Next Generation Sequencing by using Kapa kit (Roche) following the manufacturer’s instruction with one modification ...
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bioRxiv - Genetics 2023Quote: ... The molar concentration of the pool was measured using the KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA) and the pool was sequenced on one lane of the Illumina HiSeq 4000 sequencer with a 75 bp Paired-End SBS chemistry (Illumina).