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Citations for Roche :
4101 - 4150 of 6468 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... also available at addgene (plasmid 15912) (http://www.addgene.org/15912/) using the Roche Rapid ligation kit (Roche). The correct insert orientation was confirmed by NheI and SmadI (NEB ...
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bioRxiv - Immunology 2021Quote: ... Reverse: 3’-GGTCCACCAAACGTAATGCG-5’) were performed using KAPA SYBR FAST ONE-STEP qRT-PCR kits (Roche) according to manufacturer’s instructions on a Lightcycler 480 Instrument-II (Roche).
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bioRxiv - Bioengineering 2019Quote: Cultures were harvested and pelleted for total RNA extraction (High Pure RNA Isolation kit (Roche™)) ...
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bioRxiv - Microbiology 2019Quote: ... DNA concentrations were estimated using qPCR and the Kapa Library Quant Kit (Kapa Biosystems, Wilmington, MA) for Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... Manual protocols evaluated for the study were KAPA Express Extract kit (KAPA Biosystems, Wilmington, MA, USA), Promega ReliaprepTM FFPE gDNA Miniprep system (Promega ...
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bioRxiv - Neuroscience 2019Quote: ... Library quality and concentration was determined with the Kapa Illumina Library Quantification Kit (KK4854, Kapa Biosystems), and the libraries were pooled and sequenced on an Illumina NextSeq 550 with 75 base pair reads ...
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bioRxiv - Developmental Biology 2019Quote: ... The barcoded sequencing libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Sequencing libraries were loaded on a NovaSeq 6000 (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... Cell viability was measured 48 hours post-infection using the Cell Proliferation Kit I – MTT (Roche) according to manufacturer’s manual as previously described78.
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bioRxiv - Microbiology 2020Quote: ... We quantified library by qPCR with the KAPA library quantification Kit (KAPA Biosystems, Wilmington, MA, USA) on the LightCycler 480 (Roche ...
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bioRxiv - Pathology 2019Quote: ... Lysis buffer mix consisting of 100 μL Lysis buffer (Vial 1, Roche FFPET Isolation Kit # 06650775001), 100 μL dH2O ...
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bioRxiv - Immunology 2019Quote: Viral RNA was extracted from serum and CSF using the High Pure Viral RNA Kit (Roche). Semen ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative real-time PCR was conducted with LightCycler-FastStart DNA Master SYBR Green I kit (Roche) using a LightCycler 480 Real-Time PCR System (Roche) ...
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bioRxiv - Systems Biology 2020Quote: ... One microliter of this suspension was used for colony PCR (KAPA2G Robust HotStart PCR Kit, Roche). Samples with a small amplicon size were considered positive hits ...
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bioRxiv - Systems Biology 2020Quote: ... One microliter of this suspension was used for colony PCR (KAPA2G Robust HotStart PCR Kit, Roche), whereby a ∼500-bp amplicon was amplified by a primer pair in which the 3’ end of one primer was complementary to the mutant allele ...
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bioRxiv - Microbiology 2019Quote: RNAs were re-extracted from the pig feces using High Pure Viral Nucleic Acid Kit (Roche), and cDNA was synthesized with random primers using SuperScript Ⅲ First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Immunology 2019Quote: ... using the KAPA Universal qPCR Mix Library Quant Kit for Illumina (Roche Diagnostics Corp.,Indianapolis, IN). The eight capture pools were then pooled equimolar and sequenced across two Illumina HiSeq2500 v2 150bp single-end rapid lanes with a ten percent PhiX control library spike (Illumina Inc. ...
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bioRxiv - Zoology 2019Quote: ... RNA (1ug) was used to make cDNA using Transcriptor High Fidelity cDNA Synthesis Kit (Roche #5081955001) and primers (FP ...
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bioRxiv - Microbiology 2019Quote: ... The SIV3vpx particles were quantified after thawing using a reverse transcriptase (RT) assay colorimetric kit (Roche) following the manufacturer’s instructions to provide a RT ng/mL titre.
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bioRxiv - Synthetic Biology 2020Quote: ... Total RNA was ribo-depleted (KAPA Ribo-Zero RNA HyperPrep Kit with RiboErase HMR, Roche #KK8560), sheared to roughly 250 bp ...
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bioRxiv - Microbiology 2019Quote: ... Whole genome DNA sequencing libraries were generated using a Kapa HyperPlus library prep kit (Kapa Biosystems). The libraries were then run on an Illumina HiSeq 4000 platform with a HiSeq SBS kit and 150/150 paired-end reads ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryonic tail biopsies were used for rapid genotyping using KAPA Mouse Genotyping Kits (Kapa Biosystems, KK7352). All mouse protocols and experiments were approved by the Rutgers Institutional Animal Care and Use Committee ...
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bioRxiv - Genetics 2021Quote: ... Libraries were prepared using 500 ng of fragmented gDNA using KAPA Hyper-Prep Kit (KAPA Biosystems) with Agilent SureSelect XT Target Enrichment System and Human All Exon V5 capture baits (Agilent Technologies) ...
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bioRxiv - Genetics 2020Quote: ... and visualization were performed using the DIG High Prime DNA Labeling & Detection Starter Kit I (Roche) as per the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... real-time quantitative polymerase chain reaction (qPCR) (KAPA Library Quantification Kit; Kapa Biosystems, Wilmington, MA, USA), and small-scale sequencing (2 x146bp ...
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bioRxiv - Immunology 2021Quote: ... Libraries were prepared at the WVU Genomics and Bioinformatics Core using KAPA mRNA HyperPrep Kit (Roche) using 10 PCR cycles starting with 300-550 ng of RNA ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were constructed using the KAPA RNA HyperPrep kit following manufacturer’s protocol (Roche Sequencing Solutions). To enrich for SARS-CoV-2 sequence ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were constructed using the KAPA RNA HyperPrep kit following manufacturer’s protocol (Roche Sequencing Solutions). To enrich for SARS-CoV-2 sequence ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
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bioRxiv - Microbiology 2020Quote: ... amplicons were used to prepare libraries using the Kapa HyperPrep kit (Kapa Biosystems, Part Number KK8504) and further processed as described in the competition assay sequencing method ...
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bioRxiv - Microbiology 2020Quote: ... or the MagNA Pure LC Total Nucleic Acid Isolation Kit (Cat. 03038505001, Roche Diagnostic, Basel, Switzerland) following the manufacturer’s recommendations.
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bioRxiv - Evolutionary Biology 2021Quote: ... and used as input into RNA-seq library preparation using KAPA RNA hyper prep kit (Roche).
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bioRxiv - Microbiology 2020Quote: ... the DNA library of pDJB-3 plasmid prepared by KAPA Hyper Prep Kit (Roche, Basel, Switzerland) gave a pool of 150 bp paired-end reads that are destined to be assembled into a contig by the SPAdes Genome Assembler (version 3.11.0) ...
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bioRxiv - Bioengineering 2021Quote: ... 10 ng of genomic DNA was used for individual libraries using a KAPA HyperPlus Kit (Roche Sequencing Solutions ...
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bioRxiv - Cancer Biology 2021Quote: Sample library construction was performed using the Kapa HyperPlus Kits with enzymatic fragmentation (Roche, Wilmington, MA). Fragmented gDNA were used to perform end repair ...
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bioRxiv - Cancer Biology 2019Quote: ... was employed using Kapa Sybr Fast qPCR Master Mix (2x) Kit (Kapa Biosystems, Wilmington, MA, USA). RNA expression of target genes relative to GAPDH was quantified by 2ΔΔCT-method ...
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bioRxiv - Cell Biology 2021Quote: ... CFBE41o- and 16HBE14o- cells was isolated using a High Pure RNA Isolation Kit (Roche, Mannheim, Germany) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Each prepared sample used 20 µL of the SYBR green qPCR reaction kit (Roche Applied Science), which contained 2 µL of cDNA for qRT‐PCR using the following primer pairs ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µg of RNA was reverse-transcribed with Transcriptor First Strand cDNA synthesis Kit (Roche, www.roche.com). The A ...
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bioRxiv - Microbiology 2020Quote: Final pools were quantified by qPCR using Kapa library quantification kit for Illumina Platforms (Kapa Biosystems) on an ABI 7900HT real-time cycler (Applied Biosystems) ...
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bioRxiv - Genomics 2021Quote: Illumina sequencing libraries were prepared using either the Kappa HyperPlus Kit (Roche, South San Francisco, CA) (lanes 2-4 ...
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bioRxiv - Microbiology 2020Quote: ... Extraction of total DNA was performed using a DNA tissue isolation kit (Roche Diagnostics, Indianapolis, IN) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were generated using the Transcriptor First Strand cDNA Synthesis kit (Roche 04 897 030 001) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Total RNA from the lung homogenate was extracted using High Pure Viral RNA Kit (Roche, Switzerland), and the purified vRNA was used to synthesize the first-strand cDNA by reverse transcription using SuperScript VILO cDNA Synthesis Kit (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: Cell death (apoptosis) was measured using the Cell Death Detection ELISA kit (Roche Diagnostics, Madrid, Spain) while mitochondrial metabolism was assessed using the MTT assay ...
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bioRxiv - Cell Biology 2020Quote: TG content was measured with an enzymatic GPO-PAP assay kit (Cobas, Roche/Hitachi, Tokyo, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent TUNEL staining was performed using the Fluorescein In Situ Cell Death Detection Kit (11684795910, Roche), with the fluorescein detected by antibody staining using rabbit anti-FITC ...
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bioRxiv - Cell Biology 2020Quote: ... TUNEL staining was performed according to manufacturer’s guideline (In-Situ Cell Death Detection Kit, Fluorescein, Roche). All staining was performed on 3 hearts per group ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries (500 ng total RNA) were prepared using the KAPA Hyperprep mRNA-Seq Kit (KAPA Biosystems). cDNA fragments were ligated to indexed library adapters (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... Ribosomal RNAs depletion and libraries preparation were conducted using KAPA HyperPrep kit with Ribo-Erase (Roche) according to manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... The site-directed mutagenesis was performed using the KAPA HiFi HotStart ReadyMix kit (Roche, Cat# KK2601) by restriction enzyme-free cloning procedure48 ...