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Citations for Roche :
4051 - 4100 of 6468 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4°C with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche #11093274910) in MABT blocking buffer with 1% sheep serum ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were gradually washed into SSC buffer and then into PBT before overnight incubation with an anti-DIG primary antibody at 1:5000 (Roche #11093274910). Embryos were washed in PBT and then staining buffer before developing in BM Purple staining solution (Roche #11442074001) ...
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bioRxiv - Cell Biology 2022Quote: ... 25 µL of these samples were resolved by SDS-PAGE and analyzed by western blotting with α-HA3 tag antibody (#3F10, Roche) for western blotting ...
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bioRxiv - Microbiology 2023Quote: ... and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% bovine serum albumin (BSA)/TBS-T ...
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bioRxiv - Plant Biology 2023Quote: ... Primary and secondary antibody dilutions used for immune detection were as follows: α-HA(rat)-1:2,000 (Roche Diagnostics, Basel, Switzerland), α-GFP(mouse)-1:1500 (Roche Diagnostics ...
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bioRxiv - Neuroscience 2023Quote: ... we incubated slices with the secondary antibody (Alexa 488 donkey-anti-chicken, Immuno Jackson, 703-545-155) and DAPI (Roche, 10236276001) for 2h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Then embryos were incubated overnight at RT in AP-conjugated anti-DIG antibody (Roche, 1:1000 diluted in the blocking buffer). On the third day ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated overnight at 4°C in 1% goat serum in MABT with 1∶5000 alkaline phosphatase-coupled anti-Digoxigenin antibody (Roche Diagnostics). On the third day ...
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bioRxiv - Physiology 2024Quote: ... the samples were blocked with 5% skim milk in PBS-DEPC for 1 hour at 4°C and incubated with anti-DIG antibody (Roche Diagnostics) diluted 1:10,000 in the blocking buffer for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4°C against either HA (1:5000, Roche 12013819001), FLAG (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... unconcentrated cell culture supernatants were incubated with a rabbit monoclonal anti-glycodelin antibody (clone 12C9, kindly provided by ROCHE, Penzberg, Germany) for 1 h at room temperature prior to incubation with immune cells ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extract preparation and immunoblotting of Chlamydomonas cultures were performed as described previously (46, 48) with the following modifications: Antibody dilutions were: rat-anti-HA (1:5000) (3F10, Roche, Switzerland), Anti FLAG rabbit polyclonal (Rockland 600-401-383 ...
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bioRxiv - Genetics 2024Quote: ... Hybridizations of the probe with the RNA were detected with an alkaline phosphatase-conjugated antibody (1:5000; anti-DIG-AP, Fab Fragments, Roche, 11093274910). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 1.5h in PBSTw-1%BSA before being incubated with anti-DIG-POD antibody (Roche, 1:2000) and other primary antibodies o/n at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Microbiology 2020Quote: ... and then labelled using the DIG High Prime DNA Labelling and Detection Starter Kit I (Roche).
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bioRxiv - Microbiology 2020Quote: ... Ziehl-Neelsen staining was performed using automated Benchmark Special Stains instrument and dedicated staining kit (Roche). Photos of immunohistochemical staining and Ziehl-Neelsen staining were taken using AxioCam HRc (Zeiss ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was converted into cDNA using the High Capacity cDNA Reverse Transcription Kit (Roche #4368814) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... followed by exome target capturing with the SeqCap EZ exome probes V3 kit (Roche, Basel, Switzerland). Cluster generation was carried out on a cBOT system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: Intracellular ATP levels were determined by an ATP Bioluminescence Assay Kit (Roche Applied Science, Mannheim, Germany), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... and 250ng was reverse transcribed into cDNA by Transcriptor First Stand cDNA Synthesis kit (Roche Diagnostics). Quantitative PCR reactions were performed with fast Start Universal Probe Master kit and UPL-probe system in a LightCycler 480 Instrument (Roche Diagnostics ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from AEC bronchial brushings was isolated by High Pure RNA isolation kit (Roche Diagnostics) and 250ng was reverse transcribed into cDNA by Transcriptor First Stand cDNA Synthesis kit (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg RNA samples were reverse transcribed using a Transcriptor First Strand cDNA Synthesis Kit (Roche). RT-PCR was performed using a CFX Connect Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... and reverse transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Sequencing libraries were prepared using the LTP library preparation kit for Illumina platforms (Kapa Biosystems, Germany). The libraries were then processed for PCR (7 cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or FITC-labeled antisense probes were synthesized using an RNA Labeling Kit (SP6/T7; Roche). The staining was revealed with NBT/BCIP or INT/BCIP substrate (Roche) ...
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bioRxiv - Genomics 2021Quote: ... Final libraries were PCR-amplified during 5 cycles with Kapa HIFI PCR kit (Roche, Basel, Switzerland) before standard library quality control with standard sensitivity NGS Fragment kit (Agilent ...
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bioRxiv - Cancer Biology 2021Quote: ... The libraries were quantified using the Kapa Library Quantification Kit Illumina Platforms (Kapa Biosystems, Boston, MA). The denatured and diluted libraries were sequenced on a NextSeq 550 System (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... and were immediately subjected to reverse transcription with the Transcriptor First Strand cDNA Synthesis Kit (Roche). Each representative cDNAs of il-6 ...
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bioRxiv - Developmental Biology 2021Quote: ... All indexed libraries were quantitated by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems #KK4854). Samples were sequenced on an Illumina NovaSeq using 100bp paired-end sequencing or an Illumina HiSeq 2500 instrument using 75 bp single-end reads ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR enriched (8-10 cycles) using the KAPA Hyper Library Preparation kit (KAPA Biosystems, #KK8504). eCLIP libraries were prepared as described above according to (Van Nostrand et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Apoptotic cells were identified by using an in situ cell death detection kit (Roche, catalog #11684795910) according to the manufactureŕs instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The digoxigenin-labelled antisense RNA probes were produced using a DIG RNA labeling kit (Roche, #11277073910) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All 20 dpf libraries were prepared using the KAPA stranded mRNA-seq kit (KAPA Biosystems 2016) at the High Throughput Genomic Sequencing Facility at UNC Chapel Hill and sequenced on one lane of Illumina 150PE Hiseq4000 (McGirr and Martin 2018) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were constructed from the fragmented DNA using the KAPA HyperPrep Kit (Kapa Biosystems, South Africa) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Large RNA libraries were constructed using KAPA Stranded RNA-Seq Kits with RiboErase (Cat. # 07962282001, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled antisense Rp-BicC RNA probes were synthesized using the RNA-Dig Labeling kit (Roche). In situ hybridization was carried out in 4% PFA fixed ovaries stored in PBT at 4°C ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... Riboprobes for in situ hybridization were synthesized using the DIG RNA labelling kit (Sp6/T7, Roche) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA (1 µg) was reversed transcribed with the Transcriptor First Strand cDNA Synthesis kit (Roche). Fragments of fz3 and wg were amplified and used for the templates for synthesizing antisense probes ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... This was carried out using the DIG RNA Labeling Kit (SP6/T7) (Roche; Cat. No. 1175025) according to the manufacturer’s instructions (13µL template ...
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bioRxiv - Neuroscience 2021Quote: ... Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7) (Roche) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The library was prepared by ligation of indexed adapter sequences using the KAPA HyperPrep Kit (Roche). Sequencing was performed using Illumina MiSeq (2 x 300-bp paired-end reads) ...
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bioRxiv - Molecular Biology 2020Quote: ... The concentration of the RNA-seq library was quantified by KAPA Library quantification kit (KAPA BIOSYSTEMS). mRNA sequencing was performed on a HiSeq 2000 platform (Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... The concentration of the ChIP-seq library was quantified by KAPA Library quantification kit (KAPA BIOSYSTEMS). ChIP sequencing was performed on a HiSeq 2000 platform (Illumina).
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bioRxiv - Cell Biology 2022Quote: ... libraries were then quantified by qPCR using the KAPA Library Quantification Kit KK4835 (cat. 07960204001, Roche) prior to amplification with Illumina’s cBot ...
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bioRxiv - Genomics 2020Quote: ... concentrators and purified in large volume columns (High Pure Viral Nucleic Acid Large Volume Kit, Roche) using 10X (2.5 ml ...
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bioRxiv - Genomics 2020Quote: ... the quantification of the prepared libraries was performed using a KAPA Library Quantification Kit (KAPA Biosystems). They were sequenced on a HiSeq 1500 (Illumina ...