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Citations for Roche :
4051 - 4100 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Cells were pelleted by centrifugation and protein was extracted with 2M NaOH/10mM BME supplemented with a protease inhibitor tablet (Roche). 55% Trichloroacetic acid was then added to precipitate protein ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were dissolved in 200 μl of Laemmli sample buffer with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, Mannheim, Germany) for 15 min at RT.
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bioRxiv - Microbiology 2020Quote: Western blot analysis of infected and uninfected erythrocytic material performed on protein extracts prepared by resuspending cells in M-PER mammalian protein extraction reagent (Pierce) supplemented with 1x protease inhibitory cocktail (EDTA free) (Roche), 300 mM benzamidine ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein extracted using RIPA buffer with protease inhibitor cocktail mix (1 Complete MINI EDTA-free protease inhibitor tablet (Roche), 25 μg/mL calpain inhibitor (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... and proteins were transferred to an activated (100% ethanol, 1 min; followed by two washing steps with water) PVDF membrane (Roche Diagnostics GmbH ...
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bioRxiv - Bioengineering 2022Quote: Ileal and colonic proteins were obtained by tissue homogenization in NP-40 Buffer with 1x cOmplete Mini Protease Inhibitor Cocktail (Roche). Protein concentrations were determined using Bradford Assay ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were incubated with Protein A/G-Agarose beads (Cat# 11 134 515 001/ 11 243 233 001, Roche), and either ARP3 or normal Rabbit IgG antibody for 2 h at 4°C with rotating ...
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bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (protein concentration at 0.35 g/L to compare with normal range 0.2-0.4 g/L range (Roche et al., 2008); cell count per mm3 was 100) ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...
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bioRxiv - Biochemistry 2023Quote: ... The protein bands were detected using horseradish peroxidase-conjugated and detection with the Lumi- Light Plus Western Blotting Substrate (Roche). Luminescence was detected with an ImageQuant LAS 4000 chemiluminescence imager (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: ... Protein purification started by quickly thawing the cells in ∼15 mL room-temperature 10mM imidazole with protease inhibitor cocktail (Roche) and freshly added 200µM PMSF ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were transferred to a PVDF membrane and immunodetected with an anti-Myc-HRP antibody (Myc-HRP, 1:5000, Roche). The remaining 10% of the immunoprecipitated samples together with 50 μg of the input were subjected to the same procedure but probed with an anti-GFP antibody (JL-8 ...
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bioRxiv - Immunology 2022Quote: The protein of interest was captured from the clarified conditioned medium by IMAC using a cOmplete His-Tag purification column (Roche) and further purified by SEC using preparative grade HiLoad 16/600 Superdex 75 columns (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... All samples were initially depleted of endogenous IgG’s using a mixture of protein A&G agarose beads (Roche AG, Switzerland). Typically ...
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bioRxiv - Cell Biology 2023Quote: Whole kidneys were homogenized and protein was extracted from 20 mg of tissue in 1 ml RIPA buffer supplemented with protease inhibitors (Roche) for 2 hrs ...
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the protein A fragment of the TAP tag or anti-HA peroxidase 1/500 (clone 3F10, Roche).
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An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: Protein flake was solubilized in urea lysis buffer (8M urea, 50mM Tris.HCl pH8, 150mM NaCl, 1X Roche cOmplete protease inhibitor) using a QSonica sonic probe with the following settings ...
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bioRxiv - Neuroscience 2023Quote: ... Five volumes of 50 mM Tris pH 8 was added and proteins were digested over night with trypsin (1:50) (Roche) at 37°C while shaking at 800 rpm ...
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bioRxiv - Cell Biology 2023Quote: 293T cells to express FLAG-tagged human RSPH proteins transiently were lysed with 1% Triton X-100 in 1X HEPES buffer with EDTA-free protease inhibitor cocktail (Roche) by rocking at 4 °C for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant was incubated with 5% v/v pre-immune rabbit sera and 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl of co-IVT protein was added along with 5 μL (bed volume) of washed HA-beads (11815016001, Roche) in 300 μL binding buffer (25 mM HEPES-NaOH [pH 7.5] ...
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bioRxiv - Biochemistry 2023Quote: ... CVF samples were normalized to a protein concentration of 1 mg/mL using sterile PBS and EDTA-free protease inhibitor cocktail (Roche) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... and real-time analysis of the thermal stability of the protein was performed by fluorescence reading (excitation: 465 nm, emission: 580 nm) on a Light Cycler 480 (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... The extraction of total proteins was performed using the PIERCE RIPA buffer (Thermo, #89900) complemented with EDTA-free cOmplete PIC (ROCHE).
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of SWR was quantified by in-gel staining using the SYPRO Orange dye against a known concentration gradient of bovine serum albumin protein (Roche).
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bioRxiv - Biochemistry 2024Quote: ... Lysates were first incubated with 5 µl anti-GFP antibody (in house) for 1 hour rotating at 4°C and subsequently mixed with 50 µl protein G-agarose (50% slurry, Roche) and incubated for 1 hour rotating at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... total proteins (g/dL) and Albumin (g/dL) levels were assessed in a ROCHE Cobas 8000 auto-analyzer (Roche Diagnostic). The adiponectin (ng/mL ...
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bioRxiv - Biophysics 2021Quote: ... Suspended membrane vesicles were diluted 1:2 in buffer EXT supplemented with protease inhibitors (Roche cOmplete EDTA-free) and 1% (w/v ...
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bioRxiv - Immunology 2021Quote: ... slices were blocked for 30 min (2% BSA, 0.1ug/ul Salmon Sperm, 0.5% Saponin, 1 U/µl protector RNase inhibitor (Roche) in 3X SSC ...
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bioRxiv - Developmental Biology 2019Quote: ... third-instar larvae were dissected in PBS and incubated with 5-bromo-2’-deoxy-uridine (10 μM, Roche) for 45 min ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of DNA were used per reaction with the FastStart Universal SYBR Green Master mix (Rox) (Roche) and run on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of 2× SYBR Green Real-time PCR Master Mix (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Neuroscience 2019Quote: ... for 12 concentrations in sample buffer (0.1% BSA/0.05% Tween/PBS, 2 tablets of cOmplete Protease Inhibitor Cocktail (Roche Diagnostics Corporation ...
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bioRxiv - Genomics 2019Quote: ... Cell nuclei pellet was re-suspended in 2 mL FACS buffer (0.15 mM spermine, 0.5 mM spermidine, Complete protease inhibitor (Roche) and 20mM Na-butyrate (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... rinsed in TBS/0.1% Tween-20 and incubated overnight in blocking buffer (TBS with 2% goat serum, 0.1% blocking reagent (Roche), 0.1% Tween-20 ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified carcinoma organoids were dissociated into single cell suspension in 0.05% trypsin with 0.1% EDTA supplemented with 2 U/ml of DNase I (Roche) for 2-3 min ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche] ...
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bioRxiv - Neuroscience 2021Quote: ... The floating sections were then treated with 4,6-diamidino-2-phenylindole (DAPI, 1 μg/ml, 10236276001; Roche Diagnostics) at room temperature for 20 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Pancreas was minced into 2- to 3-mm pieces and digested with 0.2 mg/ml collagenase P (Roche) at 37 °C for 10-12 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM MOPS, pH 7.2; 2 mM EDTA; 1 mM PMSF; 1X cØmplete, mini, EDTA-free protease inhibitor cocktail, Roche). Cells were lysed by bead beating with 0.5 mm glass beads for 3×20s at 6.5 m/s in a benchtop homogenizer (Fastprep-24 ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM MOPS, pH 7.2; 2 mM EDTA; 1 mM PMSF; 1X cØmplete, mini, EDTA-free protease inhibitor cocktail, Roche). Cells were lysed by bead beating with 0.5 mm glass beads for 3×20s at 6.5 m/s in a benchtop homogenizer (Fastprep-24 ...
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bioRxiv - Neuroscience 2020Quote: ... 40 mM HEPES, pH 7.4, 2 mM EDTA, 10 mM pyrophosphate, 10 mM glycerophosphate, 0.3% CHAPS, protease inhibitors from Roche) as indicated.
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bioRxiv - Molecular Biology 2020Quote: Cell viability was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Roche, Mannheim, Germany), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Patient cfDNA was isolated from 2 mL of plasma using the semi-automated MagNA Pure 24 System (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 genomic RNA was isolated from cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... After incubating in primary antibodies for two hours and DAPI (4′,6-diamidine-2′-phenylindole dihydrochloride, Sigma Aldrich, 10,236,276,001 Roche) for the last ten minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (11465007001, Roche Diagnostics, Mannheim, Germany) was performed according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... before being detached with 40 μL of ice-cold native lysis buffer (80 mM PIPES pH 6.9, 2 mM MgCl2, 4 mM EGTA, 0.2% saponin, 5x cOmplete protease inhibitor cocktail Roche). The lysate was collected in 1.5 mL tube and incubated on ice for 10 min ...