Labshake search
Citations for Roche :
4301 - 4350 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
-
bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
-
bioRxiv - Immunology 2024Quote: ... NK cells were cultured for the indicated amount of time with or without human IL-2 (TECINTM; teceleukin, ROCHE), human IL-15 (247-IL/CF ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in 4 times the pellet volume of sucrose buffer (10 mM HEPES, pH 7.5, 250 mM sucrose, 2 mM magnesium acetate, 1X cOmplete EDTA-free protease inhibitor cocktail [Roche]) and lysed by douncing at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... and resuspended in solubilization buffer (50 mM HEPES/KOH pH 7.5, 200 mM NaCl, 2 mM MgAc2, 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche] ...
-
bioRxiv - Developmental Biology 2023Quote: ... RPE explants were collected in 60 μl of lysis buffer (Tris-HCl 62.5 mM, SDS 2%, pH 6.8) with protease (Roche, #1183617001) and phosphatase inhibitors (ThermoFisher ...
-
bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
-
bioRxiv - Developmental Biology 2022Quote: ... 1% NP40, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 0.1mM Na3VO4, 4 μg/ml leupeptin, one Roche cOmplete™ protease inhibitor tablet ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.75%/0.15% dodecyl maltoside/cholesteryl hemisuccinate (DDM/CHS) with 2 mg/ml iodoacetamide and a Protease Inhibitor tablet (Roche). After 4 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were transfected with Tau P301L-V5 encoding full length human tau with P301L mutation and V5 tag (GKPIPNPLLGLDST) and TFEB-GFP at a 2:1 ratio of tau:TFEB (X-tremeGENE 9, Roche). 24 hours later the media was changed and 40 µL of Pff was added to the culture along with 200 nM Bafilomycin (Sigma ...
-
bioRxiv - Physiology 2023Quote: ... approximately 15 mg of pulverized mouse intestinal tissue was solubilized in ice-cold urea Lysis buffer (8 M urea in 50 mM Tris, pH 8.0, 40 mM NaCl, 2 mM MgCl2, 1x Roche cOmplete ULTRA EDTA-free protease inhibitor mini tablet ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were lysed with TNE-lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and complete Protease Inhibitors, Roche) for 30 min on ice then spun down at 11,000 rpm for 10 min ...
-
bioRxiv - Genetics 2023Quote: ... incubated the islets for 10 min at 37°C in 2 mL PBS with 4U Dispase I (Roche Diagnostics) / 2U DNase I (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... PCR reactions with a total volume of 25 μL included: 2× KAPA HiFi Hot Start Ready Mix (KAPA Biosystems), a final concentration of 0.1 mM for each primer and 7.5 μL of DNA sample ...
-
Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... 100mM sodium chloride) for at least 2 hours and then incubated in anti-fluorescein-POD Fab fragments (Roche, 11207733910) diluted at 1:400 overnight at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 µl of cDNA were used for Real-Time PCR with self-designed primers and SYBR green reaction (Roche). qRT-PCR reactions were performed in duplicates including non-template controls on a QuantStudio 3 Real-Time PCR system (Applied Biosystems) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and thus we dechorionated Stage 24-25 embryos (or 2 to 2.5 dpf) pupfish by incubating them on 20 mg/ml Pronase (Roche) in water for 2.5 hours ...
-
bioRxiv - Microbiology 2023Quote: ... Each 20 µl qPCR consisted of 10 µl 2× Light Cycle 480 SYBR Green I Master (Roche Applied Sciences), 1 µM each primer ...
-
bioRxiv - Neuroscience 2023Quote: ... rat cortical neurons cultured as described above were lysed directly in buffer (50 mM HEPES pH 7.0, 2% SDS, 1 mM EDTA plus protease inhibitor mixture (PIC, Roche) and 20 mM MMTS (to block free thiols) ...
-
bioRxiv - Biochemistry 2023Quote: ... Ndel1) were diluted in assay buffer at concentrations indicated in Figure legends supplemented with 2 mM AMP-PNP (Roche). Prior to image acquisition ...
-
bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hr and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
-
bioRxiv - Immunology 2023Quote: ... lysed by ice-cold RIPA buffer (50 mM Tris pH8, 150 mM NaCl, 2 mM EDTA, 0.5% NP40) supplemented with protease inhibitors (Roche) and by sonication ...
-
bioRxiv - Developmental Biology 2023Quote: ... The slides were washed and detection was performed at pH 9.5 by incubating in nitro blue tetrazolium and 5-bromo-4-cholro-2-indoyl phosphate solution (Roche) as per manufacturer instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... 2% (w/v) SDS and immediately prior to use supplemented with cOmplete™protease inhibitor cocktail (Roche. Cat# 04693116001) and PhosStop (Roche ...
-
bioRxiv - Cell Biology 2023Quote: ... The clarified lysate was then diluted 3.75 times using dilution buffer (10 mM Tris–HCl, pH 7.4, 2 mM EDTA, 1 mM DTT, protease inhibitor cocktail tablet (cOmplete, Roche) and phosphatase inhibitor (phosSTOP ...
-
bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hour and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
-
bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Bacteria were harvested by centrifugation and resuspended in 20 mL of lysis buffer (50 mM Tris pH 7.5, 300 mM NaCl, 2 mM MgCl2 and 1 mM DTT, 1X Roche EDTA-free Complete protease inhibitor cocktail) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transgenes were identified using PCR primers specific for each transgene (Suppl. Table 2) amplified using Taq DNA polymerase (Roche) and Failsafe Buffer D (Epicentre) ...
-
bioRxiv - Molecular Biology 2023Quote: ... in complementary deoxyribonucleic acid (cDNA) from 10 ng total RNA in 10 μl reactions with 2× Master Mix (Roche) in a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Biophysics 2023Quote: Cell lysis was performed by resuspending cell pellets in lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 0.5 mM DTT; 2 µM avidin, protease inhibitors cocktail (Roche) and DNAse (Roche) ...
-
bioRxiv - Molecular Biology 2023Quote: SDGC-SEC-purified stress granule cores (strain JD1370) were incubated at 0.23 A260 units/mL with 2 or 4 units of RNase H (10786357001; Roche) in 40 μL of RNase H buffer (20 mM HEPES-KOH pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
-
bioRxiv - Molecular Biology 2023Quote: Successful virus rescue was monitored with SARS-CoV-2 Rapid Antigen Test (Cat# 9901-NCOV-01G, SD BIOSENSOR, Roche) by applying 100 μL of unfiltered culture supernatant directly onto the test device.
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... cells were lysed with TNE-lysis buffer (50mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and cOmplete protease inhibitors, Roche) and prepared for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
-
bioRxiv - Microbiology 2023Quote: ... First-strand cDNA was synthesized from 2 μg of total RNA using Superscript II reverse transcriptase (Roche, Mannheim, Germany). qPCR was performed in a 20 μl reaction mixture containing 10 μl of SYBR Green Master Mix (Applied Biosystems) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 100 μl of MNase buffer (0.3 M Sucrose, 85 mM Tris, 3 mM MgCl2, 2 mM CaCl2, 2.5U of micrococcal nuclease: Roche 10107921001) was added in to each tube (0.5 millions of cells per tube) ...
-
bioRxiv - Developmental Biology 2024Quote: ... whole-exome DNA from blood or salivary samples was captured using the Nimblegen v.2 exome capture reagent (Roche) or Nimblegen SeqxCap EZ MedExome Target Enrichment Kit (Roche) ...
-
bioRxiv - Immunology 2024Quote: Five female mosquitoes were collected in 250 μL of 2× RIPA buffer complemented with protease inhibitor (Complete 1×, Roche) in tubes containing ∼20 1-mm glass beads (BioSpec) ...
-
bioRxiv - Developmental Biology 2024Quote: ... samples were incubated in 50% Hybridization Buffer (50% formamide, 2x SSC, 1 mg/ml Torula RNA, 0.05 mg/ml Heparin, 2% Roche blocking reagent ...
-
bioRxiv - Plant Biology 2024Quote: ... Samples were analyzed for glucose production rate at 2 h by using an Accu-Chek® Aviva glucometer (Roche Diagnostics Scandinavia AB ...
-
bioRxiv - Biophysics 2024Quote: ... 2 million cells were seeded in a 10-cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
-
Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... embryos were with PBT (x3, 15mins) and incubated in prehybridization buffer (50% deionized formamide, 5× SSC, pH 4.5, 2% Roche Blocking Reagent ...
-
bioRxiv - Molecular Biology 2024Quote: ... SDS (0.2%), Triton X-100 (2%), sodium deoxycholate (1%), Tris HCl (100 mM, pH 8.0)) with protease inhibitor (Roche #11836145001), then incubated on ice for 30 m ...
-
bioRxiv - Microbiology 2024Quote: ... Broncholalveolar lavage (BAL) fluids were obtained by intratracheal injection of 2 × 1 ml PBS supplemented with protease inhibitors (Roche). Serum was collected by intracardiac puncture and separated from whole blood in Z-Gel microtubes (Sarstedt) ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were lysed in 200 μL lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF, cOmplete Protease Inhibitor Cocktail, Roche), heated to 99 °C for 5 min ...
-
bioRxiv - Microbiology 2021Quote: ... pooled and quantified (KAPA library quantification kit from KAPA Biosystems), and sequenced with the MiSeq system (Illumina).
-
bioRxiv - Developmental Biology 2021Quote: ... and by qPCR using a KAPA Library Quantification Kit (Roche) for Illumina platforms ...
-
bioRxiv - Cell Biology 2020Quote: ... Revelation was performed using the Ultraview DAB revelation kit (Roche). Nuclei were counterstained with hematoxylin solution (Dako) ...
-
bioRxiv - Developmental Biology 2021Quote: ... quantified with a Kapa Library Quantification Kit (Kapa Biosystems KK4824), and sequenced on an HiSeq 2500 platform (Illumina ...