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Citations for Roche :
4001 - 4050 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... mice brain/cells were lysed in lysis buffer (20 mM Tris pH=7.5, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor cocktail (Roche). Lysate was sonicated and incubated at 4 °C for 30 min while rotating ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 0.5% Triton X-100) supplemented with fresh protease and phosphatase inhibitors (1 mM PMSF, 0.1 mM Na3VO4 and 1x PIC - Roche). Cell lysates were passed through a 1 ml syringe for mechanical disruption and centrifuged for 10 min at 13.000 rpm ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 0.5% Triton X-100) supplemented with fresh protease and phosphatase inhibitors (1 mM PMSF, 0.1 mM Na3VO4 and 1x PIC - Roche), followed by three washes with kinase buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 10% glycerol, 0.1% Triton X-100 and 2 x cOmplete EDTA-free protease inhibitor cocktail [Roche]). Cell lysis was obtained by a mechanical-pestle and sonication ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 0.5% Triton X-100, 1 mM DTT, 150 μM ZnSO4, 1 mM PMSF, 1xEDTA-free protease inhibitors [Roche]) overnight on a rotating wheel at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 20% glycerol, 0.2% Triton X-100, and 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... containing Triton(R) X-100 (Nacalai Tesque, Cat# 35501-15) and cOmplete™ protease inhibitor cocktail (Roche, Cat# 4693116001). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... Pelleted cells were resuspended in Lysis buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2 1% Triton X-100, protease inhibitors (Roche Complete Inhibitor cocktail ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were washed in a serial SSC buffer and formamide solution and then preincubated in buffer 1 (100 mM Tris-HCl, pH 7.5, 150 mM NaCl) with a 1% blocking reagent (Roche) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed twice in ice-cold PBS and resuspended in 600 uL NLB (1xPBS, 300 mM NaCl, 1% TritonX-100, 0.1% Tween-20) freshly supplemented with 1x Complete protease inhibitors (Roche), and 1x PhosSTOP (4906845001 ...
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bioRxiv - Plant Biology 2024Quote: ... 100 nM of each forward and reverse primer (Supplementary Table S4) and 125lllng λ-DNA (Roche Diagnostics, Vilvoorde, Belgium) was mixed with 2lllμL of a 12x diluted sample cDNA ...
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bioRxiv - Microbiology 2023Quote: ... and 0.1% Triton X-100 supplemented with 1 mM PMSF and one Complete Protease Inhibitor tablet (EDTA-free; Roche), and then centrifuged at 13,000 × g for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Mixed-stage transgenic worms expressing ectopic GFP::EGO-1 were collected and resuspended in equal volumes of 2x lysis buffer (50 mM Tris-HCl [pH 8.0], 300 mM NaCl, 10% glycerol, 1% Triton X-100, Roche ®cOmplete EDTA-Free Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2023Quote: ... samples were collected and washed twice in 1x phosphate buffered saline (PBS) and resuspended in 100 mM Tris-HCl (pH 8.5) containing a protease inhibitor cocktail tablet (Roche). Following the addition of sodium dodecyl sulfate (SDS ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM MOPS) pH 7.0 plus 0.1% Triton X-100 and 1x cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany) on ice ...
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bioRxiv - Molecular Biology 2023Quote: 3-5 day old males of the indicated genotypes and experimental groups were homogenised in 150µl of homogenisation buffer (0.2%Triton X-100 supplemented with 1 x complete mini EDTA-free protease inhibitor (Roche) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were lysed using 200 μL/well cell lysis buffer (100 mM Tris-HCl pH 8.8, 1 % SDS) and protease inhibitors (cOmplete, Roche) on ice for one h ...
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bioRxiv - Neuroscience 2023Quote: ... The cortical tissue was suspended in 600μl lysis buffer (50mM Tris-HCl, 140mM NaCl, 10% Glycerol, 0.5% IGEPAL, 0.25% Triton X-100, protease inhibitor cocktail (Roche, 4693124001)) and manually homogenized ...
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bioRxiv - Neuroscience 2023Quote: ... NaCl 150 mM supplemented with 1% Triton X-100 in the presence of protease inhibitors (Complete, Roche Diagnostics GmbH). Fusion proteins were purified on glutathione-Sepharose (Cytiva ...
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bioRxiv - Genomics 2023Quote: ... and the pellet resuspended in 100 µl of ice-cold RIPA lysis buffer containing 1× protease inhibitor cocktail (Roche), incubated for 30 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... using 100 nL containing (final concentration) 1 × Light Cycler 480 SYBR® Green I Master Mix (Roche Inc., USA), nuclease free PCR-grade water ...
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bioRxiv - Biophysics 2022Quote: ... 5 EDTA and 1% Triton X-100) supplemented with cOmplete™ Mini-EDTA free protease inhibitor cocktail (Roche, Germany). Lysates were incubated 5 min on ice and centrifuged 10 min - 14000 x g at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting cell pellet was resuspended in a lysis buffer of Buffer A + 0.5% Triton X-100 containing 5% protease inhibitor cocktail cOmplete (Roche, 118614500 ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95°C for 5 min in SDS lysis buffer (5% SDS in 100 mM triethylammonium bicarbonate pH 8.5, complete EDTA free protease inhibitor cocktail (Roche), phosphatase inhibitor cokatail-2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
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bioRxiv - Plant Biology 2023Quote: ... 5% glycerol, 50mM KCl, 5 mM EDTA, 0.1% Triton X-100, 5 mM DTT and 1 × protease inhibitor cocktail [Roche]). The homogenized lysates were incubated at 4 °C for 1 hour for western blot analysis of eIFiso4G1 or overnight for mass spectrometry analyses with 50 µl of prewashed immobilized γ-aminophenyl-m7GTP (C10-spacer)-agarose beads (Jena Bioscience) ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in immunoprecipitation (IP) buffer (1× PBS, 0.1% Triton X-100, and cOmplete EDTA-free protease inhibitor cocktail [Roche]) and then disrupted by three pulses of sonication for 5 s ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.0, 1 mM EDTA, 10 mM DTT, 0.05% [v/v] Triton X-100 and 1x Protease Inhibitor Cocktail [Roche]). Crude leaf extracts were incubated for 2 h at 4 °C on a turning wheel and centrifuged at 16.900 g for 5 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... were equilibrated with dilution buffer (20 mM Tris, 150 mM NaCl, 0.5% Triton X-100 and protease inhibitor cocktail (Roche), pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in PBS supplemented with 0.5% (v/v) Triton X-100 and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche) (PI) ...
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bioRxiv - Microbiology 2023Quote: 293T cells transfected with pCAG AcGFP-FOS or pCAG AcGFP-JEVcore-FOS were incubated for 48 h and lysed in lysis buffer (20 mM Tris-HCl [pH = 7.4], 135 mM NaCl, 1% Triton X-100, 1% glycerol, and protease inhibitor cocktail tablets [Roche]). The cell lysates were incubated with Strep-Tactin Sepharose (IBM GmbH ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in ice cold lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0) supplemented with protease inhibitors (Roche) and incubated on ice for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... chopped samples were transferred to a 15 ml Dounce Homogenizer with 5ml homogenisation buffer (100 μM of DTT [Sigma–Aldrich, St. Louis, MO, USA], 0.1% Triton X-100 [Sigma–Aldrich], 2X EDTA Protease Inhibitor [Roche, Basel ...
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bioRxiv - Biochemistry 2023Quote: ... washed with 1 x PBS and resuspended in 100 μl of SoTE buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.15 % Triton X-100, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Genetics 2023Quote: VSMCs and myofibroblasts were scraped in phosphate-buffered saline (PBS) supplemented with protease inhibitor cocktail (1:100, Roche, 11836145001) and phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Molecular Biology 2024Quote: ... with Triton(R) X-100 (Nacalai Tesque, Cat# 35501-15) and cOmplete™ protease inhibitor cocktail (Roche, Cat# 4693116001). Proteins were isolated from the supernatant ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates were prepared in a lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, EDTA-free protease inhibitor cocktail [19543200; Roche]). After centrifugation at 17,700 × g for 10 min ...
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bioRxiv - Physiology 2024Quote: ... Pellets were resuspended with binding buffer (100 mM HEPES, 1 mM EDTA, 1% SDS, pH 7.4, and protease inhibitor cocktail from Roche) by vortexing for 20 min at room temperature ...
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bioRxiv - Physiology 2024Quote: ... 100 nanograms of total RNA were used for library preparation with the KAPA total RNA Hyperprep Kit (KK8581) (Roche). Each resulting uniquely dual-indexed library was quantified and quality accessed by Qubit and Agilent TapeStation ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using NP-40 lysis buffer (100 mM Tris pH 8.0, 1% NP-40, 150mM NaCl) containing protease inhibitor cocktail (Roche) for 20 min with shaking on ice ...
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bioRxiv - Microbiology 2024Quote: ... and lysed in 1 mL of 1% Triton X-100 lysis buffer (50mM Tris-HCl [pH 7.4], 150mM NaCl) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 20 min on ice ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The resulting lysate was cleared by centrifugation (13 000 g, 40 min, 4°C) and the supernatant was incubated with cOmplete His-tag purification resin (Roche, Germany) and allowed to bind overnight at 4°C with gentle agitation ...
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bioRxiv - Microbiology 2019Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... the cDNA of our samples were diluted 10 fold and 4 µl were added to a mix of 5 µl FastStart Universal SYBR Green Master (Roche, Germany), 0.4 µl of nuclease-free water and 0.3 µl of forward and reverse primer (see primer sequences in Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Two milligrams recombinant MYC and 12 μl T7-HCF-1VIC were rotated overnight at 4°C in Kischkel buffer + PIC (Roche 05056489001). Anti-FLAG M2 Affinity Gel (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... The digested DNA was separated in a 1 % agarose gel at 50 Volts for 72 hours at 4°C and transferred to a nylon membrane (Roche®) overnight ...