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Citations for Roche :
3951 - 4000 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... RNC samples were buffer exchanged into deuterated Tico buffer (100% D2O and d8-HEPES, pH 7.5) supplemented with protease inhibitors (Roche), with a final sample concentration of 7.5 μM ...
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bioRxiv - Cell Biology 2021Quote: ... mitochondria were lysed in lysis buffer (50 mM Tris pH 7.4, 100 mM KCl, 1 mM EDTA, 1× Complete Protease Inhibitor cocktail (Roche), 1mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... human iPSC-derived neuronal cultures and N2a cells were collected in Lysis Buffer (50 mm Tris-Base, 150 mm NaCl, 1% Triton X-100, 0.5% deoxycholic acid) with protease inhibitor (Roche) and phosphatase inhibitor cocktails II and III (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X- 100, 2 mM EDTA, 20 mM Tris, pH 8.1, 150 mM NaCl, and protease inhibitors; Roche), and then washed once with 1 ml of “high-salt buffer” (the same but with 500 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen retinas were subsequently homogenized by repetitive pipetting on ice in PBS with 0.5% TritonX-100 and cOmplete protease inhibitors (Roche). The homogenates were precleared by incubation with protein G Sepharose (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: Whole brain samples were homogenized in carbonate buffer (pH 11.5, 100 mM Na2CO3, 50 mM NaCl, protease inhibitor) using the MagNA Lyser (Roche) for 5 s at 5000 speed ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... pH 8.0) augmented with protease and phosphatase inhibitors (1:100 HALT PI cocktail, Thermofisher Sci Cat# 78440; or Roche cOmplete ULTRA [Cat# 05892970001] plus phosStop [Cat# 4906845001] tablets) ...
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bioRxiv - Neuroscience 2019Quote: Microdissections were triturated in 100 μl of lysis buffer (150 mM NaCl, 1% Triton, 100 mM Tris, pH 7.4, 10 mM EDTA, 1X protease/phosphatase inhibitor (Roche)) per 10 mg of tissue and incubated 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were washed 3x with PBS and then lysed in 1 ml TX-100 lysis buffer (1% Triton X-100, 150mM NaCl, 50 mM Tris-HCl, pH 7.4) supplemented with protease inhibitor cocktail (Roche). Cell lysates were cleared by centrifugation at 10,000 × g for 10 mins at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5% NP-40, 0.5% TX-100, 150 mM NaCl plus complete EDTA-free Protease Inhibitor Cocktail Tablets, Roche, #11697498001), diluted 2:1 in 4X Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... were incubated 15 min in lysis buffer (20 mM HEPES buffer, pH 7.0; 100 mM NaCl; 5 mM MgCl2; 1% Triton X100; protease inhibitors (Roche)) and centrifuged for 15 min at 14 000 rpm in a refrigerated microfuge ...
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bioRxiv - Microbiology 2019Quote: ... Transfected cells were washed two times in cold PBS and lysed in lysis buffer (20 mM Hepes, 100 mM NaCl, 5 mM MgCl2, 0,2 % Triton X100, 10 % Glycerol, protease inhibitors (Roche)) ...
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bioRxiv - Genomics 2019Quote: ... were lysed in 100 ul Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1%SDS, 1x protease inhibitors -Roche) and sonicated in a microtube (Covaris ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed with Low Salt buffer (50 mM HEPES pH 7.5, 140 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and lysed by sonication in prenylation buffer (50 mm HEPES, pH 7.2, 50 mm NaCl, 2 mm MgCl2, 100 μm GDP, 1× Roche complete EDTA-free protease inhibitor cocktail) ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100 plus protease and phosphatase inhibitor cocktail tablets (Complete mini, EDTA-free, Phos-STOP from Roche). Insoluble cell material was rejected by centrifugation ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was fragmented to 100-300bp and libraries were constructed using the KAPA HTP Library Kits (KAPA Biosystems). A single low input sample used the Swift Accel-NGS 2S DNA Library Kit (Swift BioSciences ...
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bioRxiv - Bioengineering 2020Quote: ... cell pellets were resuspended in 50–100 mL Strep-tag binding buffer (100 mM Tris-HCl, 150 mM NaCl, pH 8.0, sterile filtrated) supplemented with one protease inhibitor tablet (Roche), 0.1 % of Triton X-100 ...
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bioRxiv - Microbiology 2021Quote: ... 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl PH8.0, 150 mM NaCl, complete protease inhibitor [Roche]), high salt (0.01% SDS ...
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bioRxiv - Plant Biology 2020Quote: ... Pellets were resuspended in buffer (50mM Tris-HCl pH 7.0, 100 mM NaCl, and 1 tablet anti-protease cocktail (Roche) for 25 mL) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... cells were lysed with 100 µl Lysis buffer (10 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, 1% Triton X-100, Protease Inhibitor Cocktail Roche). The cell pellet was resuspended by pipetting and incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... were resuspended in 50 μl PBS following the addition of 50 μl 1% SDS in 100 mM Hepes/NaOH pH8.5 supplemented with protease inhibitor cocktail EDTA-free (Roche). Samples were heated to 95°C for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... and lysed in 1 mL of 1% Triton X-100 lysis buffer (50mM Tris-HCl [pH 7.4], 150mM NaCl) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 20 min on ice ...
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bioRxiv - Genomics 2019Quote: ... 12000g and supernatant diluted with 1 volume of Dilution Buffer (1mM EGTA 300 mM NaCl, 2% Triton x-100, 0.2% sodium deoxycholate, 1x protease inhibitors-Roche). Sonicated chromatin was then incubated 4h at 4°C on a rotating wheel with 6 ug of antibody conjugated to 20 µl of Dynabeads ...
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bioRxiv - Neuroscience 2020Quote: ... The beads were then washed 2 × with washing buffer (20 mM Tris, 150 mM NaCl, 0.5 % Triton-X-100, 2 mM EGTA, complete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... the beads were washed 3 × in washing buffer (20 mM Tris, 150 mM NaCl, 0.5 % Triton-X-100, complete protease inhibitor cocktail (Roche)) that was either substituted with 2 mM EGTA or 0.5 mM CaCl2 and 1 mM MgCl2 ...
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bioRxiv - Neuroscience 2020Quote: ... then the beads were washed 3 × with washing buffer (20 mM Tris, 150 mM NaCl, 0.5 % Triton-X-100, complete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... the beads were washed 3 × in washing buffer (20 mM Tris, 150 mM NaCl, 0.5 % Triton-X-100, complete protease inhibitor cocktail (Roche)) that was either substituted with 2 mM EGTA or 100 μM CaCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... and 100 mM Tris-HCl pH 7.5 (Quality Biological, USA, #351-006-101) and protease inhibitors (Roche, Germany, #118636170001) and sonicated using a Sonic Dismembrator (Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Pelleted cells were re-suspended in lysis buffer (phosphate buffered saline containing 0.5% Triton-X 100 and complete protease inhibitors [Roche]) and DAPI was added (final concentration 5μM ...
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bioRxiv - Neuroscience 2021Quote: ... PFA was removed and cells were washed 3x with PBS then blocked in a buffer containing 0.2% Triton X-100 (Roche) in 50% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were homogenized using mortar/pestle with liquid nitrogen and then lysed in ice-cold RIPA buffer (50mM Tris-HCL pH=7.4, 150mM NaCl, 1% Triton X-100, 0.1% SDS) (Beyotime, P0013B) freshly supplemented with protease inhibitor (Roche, 11836145001) and phosphatase inhibitor (Roche ...
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bioRxiv - Genetics 2021Quote: ... and the cell pellet resuspended in cold lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, 1% Triton X-100, cOmplete protease inhibitor cocktail, Roche) and incubated on ice for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl; 10 mM EDTA; 0.1% SDS; 1% Triton X-100; 1% deoxycholate; 1x complete protease inhibitor cocktail [Roche]), clarified and 20 μg total protein were used for SDS-PAGE followed by electroblotting ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 100 µL Lysis Buffer (10 mM Tris-HCl pH 7.5, 10 mM EDTA, protease cocktail inhibitor (Roche), 0.1 mg/mL lysozyme ...
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bioRxiv - Neuroscience 2020Quote: Whole zebrafish brains were placed in 100 µL of 1X Complete Protease Inhibitor Cocktail (Roche Holding AG, Basel, Switzerland) in RIPA buffer (Sigma-Aldrich Corp ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for in donkey anti-mouse FITC antibody diluted in 10 X blocking buffer (1:100; Roche) + 0.3 % Triton-X100 for 1 h ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
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bioRxiv - Developmental Biology 2022Quote: ... A small amount of each organ was cut and homogenized in ice-cold lysis buffer containing 0.3% Triton X-100 in PBS with protease inhibitor (complete mini EDTA free protease inhibitor, Roche). The suspension was centrifuged for 30 min ...
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bioRxiv - Cell Biology 2022Quote: all the samples were lysed with High Salt Buffer (50 mM Tris pH 7.5, 300 mM NaCl, 1% Triton X-100, Protease inhibitors (Roche), Phosphatase inhibitors Halt cocktail ...
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bioRxiv - Cell Biology 2022Quote: ... cells were obtained by lysing cells in PBS containing 0.1% Triton X-100 (PBS/TX) as well as protease and protein inhibitor cocktails (Roche) for 5 min on ice ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were washed with 200 μl of solution A (with 10 mL of Tris-HCl at 100 mM pH 7.4 and one cOmplete Mini EDTA-free tablet of protease inhibitor cocktail from Roche) and pelleted with benchtop centrifuge ...
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bioRxiv - Cell Biology 2022Quote: H3K4me3-immunoprecipitated genomic DNA was diluted 1:100 and Chip-qPCR performed with a Light Cycler 480II machine (Roche) using technical duplicates and ChIP-qPCR signals were calculated as % of input ...
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bioRxiv - Genetics 2022Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, Complete EDTA-free protease inhibitor cocktail tablets, Roche) with half volume of glass beads (Sigma ...
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bioRxiv - Plant Biology 2022Quote: 7-day-old Q28 and Q69 seedlings were lysed in lysis buffer (1% Triton X-100, 50 mM Tris–HCl pH 8.0) supplemented with protease inhibitor cocktail (Roche) and 25 mM N-ethylmaleimide ...
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bioRxiv - Molecular Biology 2022Quote: ... 60 mM KCl, 15 mM NaCl, 1 mM CaCl2, 0.9% Triton X-100, and 13 protease inhibitor cocktail [Roche]). After homogenization ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 1% SDS, 0.1% Na deoxycholate, 1% Triton X-100, and 13 protease inhibitor cocktail [Roche]) was collected as nuclear proteins ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were collected as the cytoplasmic fraction and pellets were resuspended in 200 µL cold nuclear lysis buffer (20 mM HEPES pH 7.9, 100 mM NaCl, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated cells were scraped into lysis buffer (10 mM Tris pH 7.6, 1% Triton X-100, 150 mM NaCl, protease inhibitor (complete, without EDTA (Roche), ½ tablet per 20 mL) ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections from proximity labeling experiments were lysed and de-crosslinked in 100 mM Tris-HCl buffer (pH = 8.0) with 2% SDS and protease inhibitor (Roche) at 95 °C for 45 min with constant shaking ...