Labshake search
Citations for Roche :
351 - 400 of 458 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated with 1 nM DIG (Digoxigenin)-labeled Telomere probes in Hybridization solution (DIG Easy Hyb, Roche) at 42°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche, #11175025910). Probes were then cleaned with MegaClear Kit (Thermo Fisher ...
-
bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR product was used to synthesize in situ probe by the addition of DIG-labeled UTP (Roche) plus the appropriate RNA Polymerase T7 or Sp6 (NEB) ...
-
bioRxiv - Developmental Biology 2023Quote: ... We synthesized digoxigenin (DIG)-labeled sense and antisense RNA probes using a DIG RNA Labeling Kit (Roche, Basel, Switzerland) after digestion with the appropriate restriction enzymes BamHⅠ-HF ...
-
Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafishbioRxiv - Developmental Biology 2022Quote: ... The probes labeled by digoxigenin were detected using alkaline phosphatase coupled anti-digoxigenin Fab fragment antibody (Roche, Basel, Switzerland) with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories ...
-
bioRxiv - Developmental Biology 2023Quote: ... In situ hybridization was performed as previously described19 with custom anti-sense DIG-labeled riboprobes transcribed in vitro (Roche) from amplified cDNA (see Key Resources Table for primers) ...
-
bioRxiv - Plant Biology 2024Quote: ... pH 7.5) and probed for 30 min with an alkaline phosphatase-labeled anti-digoxigenin antibody (Roche, catalog no. 11093274910). Membranes were washed twice for 15 min with washing buffer (0.1 M maleic acid ...
-
bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin labeled antisense riboprobes were synthesized by in vitro transcription using a DIG RNA labeling kit (Roche, Cat#11277073910), followed by purification using Micro Bio-Spin P-30 gel columns (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: Digoxigenin-labeled RNA antisense probes and corresponding sense probes were synthesized using DIG RNA Labelling Kit (Roche, Basel, Switzerland) and the specific primers are listed in Supplementary Table 1 ...
-
bioRxiv - Microbiology 2021Quote: ... The pellet was suspended in 100 μL of staining solution containing FITC-conjugated Annex-in-V and propidium iodide (Annexin-V-Fluos Staining Kit, Roche Molecular Biochemicals, Germany) and incubated for 15 min at 20 °C ...
-
bioRxiv - Microbiology 2020Quote: ... Disruption of the gene was confirmed by Southern hybridization analysis using the total DNA of mutant candidates digested with SalI and the digoxigenin-labeled probes (Roche), the 1.4-kb ApaI fragment carrying SLG_24970 and the 1.3-kb EcoRV fragment carrying kan.
-
bioRxiv - Cell Biology 2020Quote: The RNA probes were transcribed with the T7 polymerase and labeled using the DIG RNA labeling kit (Roche Cat # 11175025910). After the labeling reaction was complete ...
-
bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described (Pearson et al., 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910 ...
-
bioRxiv - Genetics 2022Quote: ... 10 µl of each PCR product were run in 2% agarose gels alongside digoxigenin (DIG)-labeled size markers VII and VIII (Roche), for 16 hours at 50 V then transferred to a positively charged nylon membrane (Roche ...
-
bioRxiv - Developmental Biology 2022Quote: ... Colorimetric in situ hybridization was performed using DIG-labeled antisense probes with the use of an automated platform (Ventana Discovery, Roche) with RiboMap fixation and BlueMap detection kits (Roche) ...
-
bioRxiv - Genomics 2020Quote: ... and a potential large inversion on chromosome 4 (Supplementary Table 11) were labeled with digoxigenin-11-dUTP (Roche, Indianapolis, IN), biotin-16-dUTP (Roche) ...
-
bioRxiv - Neuroscience 2020Quote: ... Transfected DRG neurons were fixed 15 min in 4 % paraformaldehyde (PFA) and hybridized with DIG-labeled GFP cRNA probes (Roche) as described previously (Merianda et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... the membranes were hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low and high stringency washes ...
-
bioRxiv - Biochemistry 2020Quote: ... Biotin-labeled CMV-RLuc-EV715’UTR (wild type or CCC mutant)-FLUC and biotin-labeled SL2 (wild type or CCC mutant) were synthesized using biotin-16-UTP (Roche). Binding reaction and pull-down were carried out as described5 ...
-
bioRxiv - Plant Biology 2020Quote: ... The amplified fragments were then used to produce digoxigenin (DIG)-labeled sense and antisense RNA probes using a DIG RNA Labeling Kit (Roche). Whole-mount in situ hybridization was performed following (39) ...
-
bioRxiv - Neuroscience 2020Quote: ... Biotin-labeled probes were synthesized by substitution of DIG RNA labeling mix with biotin RNA labeling mix (Roche, Mannheim, Germany). The probes were precipitated by 4 M LiCl and 100% ethanol ...
-
bioRxiv - Neuroscience 2022Quote: ... high specific-activity RNA probes were produced from a linearized plasmid (HindIII) and labeled using the Fluorescein RNA Labeling Mix (Roche), following manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... pre-let-7 and pre-miR-16 molecules (miRVana) were radioactively 5’-labeled with 32P isotopes by incubation with 32P-ƴATP and the phosphorylating enzyme T4 PNK (Roche) for 30 min at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... The membrane was hybridized with digoxigenin-labeled DNA probes synthesized with a PCR DIG probe synthesis kit (Roche Molecular Biochemicals) as recommended by the supplier ...
-
bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
-
bioRxiv - Biophysics 2021Quote: ... or a multiply digoxigenin-labeled tail (MT) to the coverslip coated with anti-digoxigenin (Roche Life Science, Indianapolis, IN, USA). The opposite end of the DNA was attached to a streptavidin-coated bead via a single biotin (TPM) ...
-
bioRxiv - Developmental Biology 2022Quote: Digoxigenin-labeled RNA probes (sense and antisense) were generated by transcription in vitro using SP6 or T7 RNA polymerases (Roche) as previously described (Cruz et al. ...
-
bioRxiv - Zoology 2022Quote: ... The DIG-labeled hsd3b probe was visualized by using a horseradish peroxidase-conjugated anti-DIG antibody (Roche Diagnostics, Basel, Switzerland) and TSA Plus Cy3 System (PerkinElmer ...
-
bioRxiv - Developmental Biology 2022Quote: ... The APP probe was generated using a BAC from BACPAC Resources (RP11-910G8) and labeled via nick translation with Digoxigenin-dUTP (Roche). To dual stain for protein and RNA ...
-
bioRxiv - Developmental Biology 2020Quote: ... They were then blocked in PBS with 3% BSA for 3h at RT and incubated overnight at 4°C with a peroxidase-labeled anti-DIG antibody (Roche) diluted 1:1500 in PBS with 3% BSA ...
-
bioRxiv - Developmental Biology 2021Quote: ... anti-sense digoxigenin-(DIG) labeled RNA probes were generated using an Sp6- or T7-RNA polymerase and DIG RNA labeling mix (both Roche) following the manufacturer’s protocol ...
-
bioRxiv - Genetics 2021Quote: ... Probes were generated using primers listed in Supplementary Table 1 from the G135P65476A4 BAC as described in [8] and were labeled using the DIG-Nick Translation Mix (Roche) as per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Incubation with the appropriate secondary HRP-labeled antibody was followed by detection with ECL western blotting substrate (Roche Applied Science).
-
bioRxiv - Genomics 2020Quote: ... These DNA fragments were labeled with digoxigenin (DIG) using a DIG High Prime DNA Labeling and Detection Starter Kit II (Roche), and signals were detected according to the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2021Quote: Pitx1 WISH were performed on 40-45 somite stage mouse embryos (E12.5) using a digoxigenin-labeled Pitx1 antisense riboprobe transcribed from a cloned Pitx1 probe (PCR DIG Probe Synthesis Kit, Roche), as previously described in (Kragesteen et al ...
-
bioRxiv - Plant Biology 2021Quote: ... Antisense or sense strand digoxygenin-UTP labeled RNA was generated for PEPCK and NADP-ME using a DIG RNA labeling kit (Roche). For PEPCK hybridizations ...
-
bioRxiv - Molecular Biology 2021Quote: ... were performed in Church–Gilbert buffer at 60 °C with TER1 probe (nucleotides 536–998, labeled with High Prime (Roche) and [α-32P]-dCTP) ...
-
bioRxiv - Immunology 2021Quote: ... cell nuclei with DNA strand breaks at the early stage of apoptosis were labeled using fluorescein-dUTP in the TUNEL reaction (In Situ Cell Death Detection Kit; Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Synthesized DNA specific to regions of homologous recombination (see Oligonuleotide section in Key Resources Table) was labeled with 32P (Amersham) using the High Prime labeling kit (Roche) according to manufacturer’s instructions and unincorporated 32P was removed with G-50 Sephadex Quick Spin columns (Fisher) ...
-
bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10) was labeled with digoxigenin-11-dUTP using DIG-Nick Translation Mix (Roche) or with biotin-16-dUTP (Promo Kine ...
-
bioRxiv - Genomics 2023Quote: ... The resulting UMI-labeled 16S dsDNA was then amplified using indexed p5/p7 primers and KAPA HiFi HotStart ReadyMix (Roche) (95°C for 3 minutes followed by 25 cycles of 98°C for 20 seconds ...
-
bioRxiv - Molecular Biology 2022Quote: ... Hybridization signals were detected with anti-mouse secondary antibody labeled with alkaline phosphatase using NBT/BCIP substrates (Roche Biochemical, Mannheim).
-
bioRxiv - Molecular Biology 2024Quote: ... The digoxigenin (DIG)-labeled probes were generated by PCR on H26 genomic DNA using primers and the PCR DIG labeling Mix from Roche. Probe hybridization was detected using the DIG Luminescent Detection kit (Roche ...
-
bioRxiv - Neuroscience 2023Quote: ... A dioxigenin (DIG)-11-UTP-labeled antisense riboprobe targeting the gene transcript of interest was synthesized using the DIG labelling kit (Roche) and T7 RNA polymerase80 ...
-
bioRxiv - Plant Biology 2023Quote: ... The 18S-1 probe contains a 552-bp fragment of the sugar beet 18S-5.8S-25S ribosomal RNA (rRNA) gene with the 35S rDNA (Schmidt et al. 1994) labeled with digoxigenin-11-dUTP by PCR (Roche) and the probe pXV1 for the 5S rRNA gene (Schmidt et al ...
-
bioRxiv - Plant Biology 2023Quote: ... and the probe pXV1 for the 5S rRNA gene (Schmidt et al. 1994) labeled with biotin-11-dUTP by PCR (Roche). The telomeric probe pLT11 from Arabidopsis (Richards & Ausubel 1988 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Prehybridization for 60 min and overnight hybridization were carried out with a DIG-labeled probe (1 µg) at 65 °C with DIG Easy Hyb Granules (Roche). A chemiluminescent assay was used to visualize the probe on the membrane ...
-
bioRxiv - Plant Biology 2023Quote: ... Paromomycin probe was labeled with dUTP-DIG during PCR reaction (PCR DIG probe synthesis kit, Roche; see table primers list). Membrane was pre-hybridized 1h at 50°C in a DIG eazy Hyb (Roche ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Telomere Restriction Fragments (TRF) location on the blots was compared to the DNA molecular weight marker VII DIG-labeled (Roche) to obtain the average of TRF.