Labshake search
Citations for Roche :
251 - 300 of 458 citations for FITC labeled Fibrinogen since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Labeled exosomes were diluted with 3.5 ml PBS containing 1x Complete protease inhibitor cocktail (Roche) and concentrated to 300 ul by diafiltration using an Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa cutoff (Merck UFC803024 ...
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bioRxiv - Molecular Biology 2024Quote: ... the annealed probes were purified with Quick Spin Columns for radio-labeled DNA Purification (Roche). ZNF410 full-length or ZNF410-ZF were overexpressed and harvested from COS-7 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... The krang digoxigenin-labeled probe was synthesized in vitro using DIG RNA labeling kit (Roche) with T3 (sense ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmid DNA containing EEEb1 was labeled with cyanine-5-dUTP by Nick Translation Mix (Roche), while plasmid DNA harboring each of the other nine DNA families (EEEb2–EEEb10 ...
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bioRxiv - Developmental Biology 2023Quote: ... DIG or fluorescein labeled RNA probes were synthesized by T7 and sp6 RNA polymerases (Roche) from template DNA plasmid digested by NotI or SpeI (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: ... or 20 μg of fluorescein-labeled probes (see below) for fluorescent detection (Roche, cat. #11685619910). After hybridization ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxygenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling NTP mix (Roche) and either SP6 or T7 RNA polymerase (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... Probes were synthesized from linearized DNA and labeled during synthesis with digoxigenin-11-UTP (Roche) nucleotides ...
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bioRxiv - Genomics 2024Quote: ... Specific Hydrangea satDNA probes were labeled indirectly by PCR with biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich) ...
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bioRxiv - Physiology 2024Quote: ... Digoxigenin (DIG)-labeled probes were synthesized with a DIG RNA Labeling Kit (Roche Applied Science). The paraffin sections were made as described above and subjected to in situ hybridization ...
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bioRxiv - Genetics 2024Quote: ... Digoxigenin (DIG)-labeled in-situ probes were synthesized using DIG RNA Labeling Kits (Roche, 11277073910). RNA in-situ hybridization was carried out as previously described (Thisse and Thisse ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The fluorescein-labeled probe was detected using an Anti-Fluor-POD antibody (Roche, Catalog# 11426346910), diluted 1:1500 in Blocking Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for one hour in donkey anti-mouse FITC antibody (1:200) in 0.3% Triton-X100 supplemented with 1X blocking buffer (Roche). After washing with PBS for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... 2011) or Tyramide Signal Amplification (TSA) after incubation with anti-digoxigenin-AP or anti-FITC-POD (Roche, 1:300) antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for in donkey anti-mouse FITC antibody diluted in 10 X blocking buffer (1:100; Roche) + 0.3 % Triton-X100 for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... Antisense and sense single-strand riboprobes were transcribed in vitro using DIG or FITC RNA labeling kit (Roche Diagnostics). cRNA probes for Gad155 and Slc17a756 were used.
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bioRxiv - Cell Biology 2024Quote: ... blocked cells were incubated with a FITC-conjugated anti-FLAG or anti-HA antibody in PBS buffer containing 0.1% Triton X-100 (Roche) for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled antisense Rp-BicC RNA probes were synthesized using the RNA-Dig Labeling kit (Roche). In situ hybridization was carried out in 4% PFA fixed ovaries stored in PBT at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7) (Roche) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng of labeled probe was mixed with 10 μg of human Cot-1 DNA (Roche) and 10 μg each of salmon sperm DNA and E ...
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bioRxiv - Plant Biology 2022Quote: ... gene-specific cDNA was amplified and labeled using the DIG RNA Labeling Kit (Roche, Basel, Switzerland). Pretreatment of sections ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization with digoxigenin (DIG)-labeled antisense RNA probes was conducted in DIG Easy Hyb (Roche Diagnostics) at 68°C ...
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bioRxiv - Physiology 2022Quote: ... Digoxigenin (DIG) labeled probe was synthesized using the DIG RNA labeling kit (SP6/T7) (Roche, #11175025910). Denatured DIG-labeled probe was hybridized at 55° C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Visualization of the labeled probe was done using anti-DIG-AP (alkaline phosphatase) antibodies (Roche Biochemicals) followed by histochemical enzymatic staining reagents (Roche Biochemicals) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequences were amplified from cDNA and DIG-labeled RNA probes complementary to the target mRNA (Roche). The primer sequences for synthesizing the probes are available in supplementary table 4 ...
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bioRxiv - Microbiology 2021Quote: ... The DIG-labeled probe was synthesized using the PCR DIG probe synthesis kit (11636090910, Roche, Switzerland) and the following primers ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After the reaction between DIG-labeled RNA and anti-DIG antibody conjugated horseradish peroxidase (HRP) (Roche), sections were washed 3 times by TNT buffer (100 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled cRNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics) from the following templates ...
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bioRxiv - Developmental Biology 2022Quote: ... and fluorescein-labeled probes were recognized using an anti-FLUO antibody coupled to peroxidase (11426346910, Roche). Fluorescence signal was produced using the TSA plus kit (NEL753001KT ...
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bioRxiv - Molecular Biology 2023Quote: ... The AAV2 ITR specific probe was labeled using the DIG DNA labeling kit (11175033910, Roche, Switzerland) using the following oligonucleotide ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of plasmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910), digoxigenin-dUTP (Roche 11093088910 ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection was done with alkaline phosphatase-labeled anti-DIG antibody and NBT/BCIP (Roche diagnostics, USA). Control experiments with sense probes did not give detectable signals (data not shown) ...
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bioRxiv - Neuroscience 2023Quote: ... which was labeled with fluorescein using T7 RNA polymerase and Fluorescein RNA Labeling Mix (Roche Diagnostics), and a DIG-labeled AR (ara ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid DNA was labeled by nick translation to incorporate biotin-dUTP (Roche 11093070910) or digoxigenin-dUTP (Roche 11093088910 ...
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bioRxiv - Neuroscience 2024Quote: ... The antisense probe was transcribed with SP6 polymerase using a DIG-labeled NTP mix (Roche Diagnostics).
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bioRxiv - Developmental Biology 2020Quote: ... gbx1 and pax6a digoxigenin and FITC antisense probes were generated from linearized plasmids using an RNA labelling and detection kit (Roche)87 ...
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bioRxiv - Neuroscience 2021Quote: ... Probes were detected by horseradish peroxidase (POD)-conjugated antibodies (anti-FITC-POD at 1/250 dilution, Roche; anti-DIG-POD at 1/500 dilution, Roche). Signals were amplified by biotin-conjugated tyramide (PerkinElmer ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were blocked for 30 minutes in 1% Roche Western Blocking Reagent prior to incubating overnight at 4°C with either anti-FITC with horseradish peroxidase conjugate (Roche) at a 1:2000 concentration or anti-DIG-with horseradish peroxidase conjugate (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Resulting amplicons were used as templates for the synthesis of either digoxigenin (DIG) or fluorescein (FITC)-labelled anti-sense probes by in vitro transcription using T7 or T3 polymerases (Roche) and DIG or FITC RNA labelling mixes (Roche).
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labeled RNA probes were synthesized by in-vitro transcription using the DIG RNA labeling kit (Roche). In situ analysis was performed as described previously (Cecchetti et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... PAN specific oligonucleotide probes were labeled with a DIG-Oligonucleotide tailing kit (Sigma Roche 2nd generation 03353583910) and mixed at the 1:1:1 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Digoxigenin-labeled RNA probes were generated by in vitro transcription using a DIG RNA Labeling Kit (Roche) and purified using Micro Bio-Spin® Chromatography Columns (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... The DIG-labeled probes were hybridized to the membrane in DIG Easy Hyb hybridization buffer (Roche #11603558001) at 49°C for overnight ...
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bioRxiv - Neuroscience 2021Quote: ... The probes were then labeled using a digoxigenin (DIG) RNA labeling Kit (Roche Diagnostics Corp., Indianapolis, IN). After pre-hybridization ...
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bioRxiv - Physiology 2022Quote: ... Detection of DIG-labeled probes was performed by incubating with anti-DIG antibody (1:2000, Roche #11093274910) in 0.1% PBST overnight at 4° C ...
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bioRxiv - Developmental Biology 2020Quote: Riboprobes were made using T3 and T7 polymerases and a digoxigenin-labeled ribonucleotide mix (Roche, Cat.# 11277073910) using PCR templates ...
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bioRxiv - Cell Biology 2021Quote: ... and digoxigenin-labeled RNA probes were transcribed using a digoxigenin-UTP in vitro transcription labeling mix (Roche) and either T7 or T3 RNA polymerase (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... and used as template to generate DIG-labeled in situ probes with SP6/T7 polymerases (Roche, # 10999644001). Whole-mount in situ hybridization was done according to Lécuyer et al ...
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bioRxiv - Molecular Biology 2022Quote: ... probes were digoxigenin-(DIG) labeled by using the DIG RNA Labeling Kit (Roche Co. Ltd, Basel, Switzerland). The female flower buds collected at 1 DAP were infiltrated with the FAA fix solution (90 ml of 50% (v/v ...
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bioRxiv - Cell Biology 2022Quote: Digoxin-labeled RNA probes were in vitro transcribed as per manufacturers’ instructions (DIG RNA labeling Mix, Roche; T7 polymerase ...