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Citations for Roche :
351 - 400 of 3510 citations for 4 Chloro 2 iodothieno 2 3 b pyridine 5 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... S-phase Synchronous HeLa S3 cells were washed with ice-cold 1× PBS and lysed in a swelling buffer (20 mM HEPES, pH 7.5, 2 mM MgCl2, 5 mM KCl, 1 mM Dithiothreitol [DTT], and protease inhibitor cocktail [Roche; #11836170001]) supplemented with energy-regenerating mixture ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed using 2-5 times the volume of RIPA lysis buffer supplemented with 1x protease inhibitor cocktail (Roche), PMSF (1 mM ...
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bioRxiv - Microbiology 2021Quote: ... the remaining dermis was washed in Ca2+ and Mg2+ free PBS 5 times and incubated in a digestion buffer containing 2 mg/ml collagenase A (Roche), 100 µg/ml of DNase I (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from 58 macro-dissected regions of slides G1-5 in samples UH1-UH16 and 30 regions of slides F1-5 in samples UH17-UH23 and UH25-UH27 (Supplementary Table 2) using the High Pure FFPE RNA isolation kit (Roche). For UH4 ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 24 hours in the presence of SARS-CoV-2 specific MPs [1 μg/mL] or 5 μg/mL phytohemagglutinin (PHA, Roche) in 96-wells U-bottom plates at 1×106 PBMCs per well ...
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bioRxiv - Genomics 2020Quote: ... was synthesised commercially and then labelled with digoxigenin-11-2’-deoxyuridine-5’-triphosphate (DIG-11-dUTP) using terminal transferase (Roche) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... water was exchanged for 100 µL water with 5 µM L-012 (WAKO chemicals) and 2 µg/mL horseradish peroxidase (Type II, Roche). After the leaf discs were treated with 1 µM 3-OH-C10:0 (stock in MeOH ...
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bioRxiv - Molecular Biology 2022Quote: ... The loci of interest were first amplified with 15 cycles of PCR from 2 μL (∼100 ng) of genomic DNA eluate using a 5 μL Kapa HiFi HotStart polymerase reaction (Roche). The first PCR was then diluted with 25 μL of DNAse-free water ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-10 5 micron sections were extracted using the High Pure FFPE RNA Isolation kit (Roche Life Sciences, Penzberg, Germany) under RNase free conditions following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... with probe prepared by nick translation of a double-stranded PCR product bearing the bcd ORF using alkali-stable digoxigenin-11-2’-deoxyuridine-5’-triphosphate (Roche), visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Immunology 2023Quote: ... tumor tissue was dissected into approximately 1– 5 mm3 fragments and digested with collagenase Type D (2 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 5 mM EGTA, 1 mM DTT, 2 mM MgCl, and one EDTA-free protease inhibitor cocktail tablet; Roche) and sonicated in an ice bath for 6 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... glands were minced into paste and incubated in 5 mL DME/F-12 medium (HyClone, #SH30023.1) with 2 mg/mL collagenase A (Roche #10103578001), 100 units/mL hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in HR buffer (50 mM Tris-HCl, 5 mM MgCl2, 250 mM sucrose, 2 mM TCEP and protease inhibitor t (Roche), pH 7.4) ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Cell Biology 2024Quote: ... then with 1 ml of Western blot buffer (2% SDS, 5% Glycerol, 50 mM Tris-HCl, 0.2 M EDTA + cOmplete protease inhibitor cocktail tablet (Roche, 11697498001) + PhosSTOP (Roche ...
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bioRxiv - Immunology 2020Quote: ... for 3 h followed by 5 mM ATP (10519987001, Roche) for 45 min were used as positive controls for caspase-1 and IL-1β blots ...
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bioRxiv - Cell Biology 2020Quote: ... 3 min) and plated onto 1.5 % agar TAP plate containing 10 µg mL-1 hygromycin B (Roche) and 20 µg mL-1 of paromomycin sulfate (Fisher BioReagents).
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bioRxiv - Molecular Biology 2021Quote: ... The pellet was resuspended in Buffer 2 (10 mM Tris-HCl pH=7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5 % IGEPAL CA-630, 10 % glycerol, supplemented with Roche Complete Protease Inhibitor Cocktail), incubated at 4°C for 10 min followed by a centrifugation step ...
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bioRxiv - Genetics 2021Quote: ... and cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C followed by a centrifugation step ...
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bioRxiv - Genetics 2021Quote: ... 30 million equivalent cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C and collected cells by a centrifugation step ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 120 μl lysis buffer (0.1 M NaOH, 0.05M EDTA, 2% SDS, 2% beta-mercaptoethanol, PhosStop (Roche), cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 350 mM NaCl, 2 mM MgOAc, 1 mM CaCl2, 15% glycerol, 0.1% Triton X-100, 0.05% 2-ME, 1x Roche protease inhibitors ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was diluted 10-fold with ChIP incubation buffer (70 mM NaCl, 10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 2 mM EDTA, 0.1% Triton, 1 x Roche Complete EDTA-free Protease inhibitor cocktail) ...
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bioRxiv - Microbiology 2020Quote: ... and BCIP (1,5-bromo-4-chlooro-3-indolyl-phosphate, Roche, Switzerland).
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bioRxiv - Molecular Biology 2021Quote: ... and 2 mg/ml DNase I (Roche) for 15–20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μL KAPA HiFi HotStart ReadyMix (Roche), 0.96 μL H2O ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of GFP antibody (Roche 11814460001) was pre-incubated with 50 μl Protein A and Protein G Sepharose (GE Healthcare 17513801 and 17061801 ...
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bioRxiv - Bioengineering 2021Quote: ... and 30 U/ml rhIL-2 (Roche) at 2×106 cells/ml in wells coated with anti-mouse CD3e (1 μg/ml ...
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bioRxiv - Genetics 2020Quote: ... CaCl2 2 mM and protease inhibitor (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mini-protease inhibitor cocktail tablets (Roche), DNase I ...
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bioRxiv - Microbiology 2021Quote: ... 2 µg of trypsin (Roche, Mannheim, Germany) was added and incubated at 350 rpm for 18 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 tablets EDTA free protease inhibitor (Roche), 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mg/ml Collagenase A (#10103586001, Roche), 2.4U/ml Dispase II (#04942078001 ...
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bioRxiv - Bioengineering 2021Quote: ... and 30 U/ml rhIL-2 (Roche) at 1 × 106 cells/ml in wells coated with anti-mouse CD3e (3 µg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... and 2% Nutridoma-CS (Roche, catalog #11363743001) (22) ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... (2 mg/kg; atezolizumab, Roche; n = 12). Group 3 received anti-TGF-β I.P ...
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bioRxiv - Cell Biology 2021Quote: ... 2% digitonin and 1x protease inhibitors (Roche) and incubated for 1 hour on ice ...
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bioRxiv - Cell Biology 2021Quote: ... 2% digitonin and 1x protease inhibitors (Roche) and incubated for 1 hour on ice before addition of 0.5% G-250 sample additive (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... and collagenase D (2 mg/mL, Roche) during 30 minutes at 37°C under 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... 100 U/mL human IL-2 (Roche), 50 ng/mL human IL-21 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... with Elecsys Anti-SARS-CoV-2 (Roche), and <1 was evaluated as seronegative according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 2× KAPA HiFi Hotstart Readymix (KAPA Biosystems), cycle conditions ...
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bioRxiv - Immunology 2021Quote: ... Human IL-2 (TECIN™ teceleukin, ROCHE, was generously provided by the NCI Biological Resources Branch ...
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bioRxiv - Immunology 2021Quote: ... and collagenase D (2 mg/mL, Roche) during 30 min at 37°C under 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2% glycerol) with protease inhibitors (Roche) and lysates were cleared by centrifugation ...
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bioRxiv - Immunology 2021Quote: ... and 2 mg/mL collagenase D (Roche), and macerated into small pieces with a scalpel ...
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bioRxiv - Genomics 2021Quote: ... with 2% BSA fraction V (Roche-Diagnostics), 50 mmol/L 2-mercaptoethanol,10 mmol/L nicotinamide (Calbiochem) ...
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bioRxiv - Cell Biology 2021Quote: ... 2% bovine serum albumin (Fraction V, Roche) in PBS for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 U/µL RNAse inhibitor (Roche) was added to all nuclei preparation buffers ...