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Citations for Roche :
501 - 550 of 3510 citations for 4 Chloro 2 iodothieno 2 3 b pyridine 5 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 2 mM PMSF and 1 % protease inhibitor (Roche). The samples were then lysed by sonication and centrifuged to collect the supernatant for the following protein purification ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 EDTA-free protease inhibitor tablets (Roche). The cell suspension was stirred for 1 hour and then sonicated for 50 seconds in 5-second pulses with 25-second waiting time at 70% amplitude using a 130 W microtip sonicator (Vibra Cell) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 EDTA-free protease inhibitor tablets (Roche). The cell suspension was stirred for 1 hour and then sonicated for 50 seconds in 5-second pulses with 25-second waiting time at 70% amplitude using a 130 W microtip sonicator (Vibra Cell) ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were washed and the secondary antibodies applied (dilutions listed below) with 4′-6-diamidino-2-phenylindole (DAPI: Cat # 10-236-276-001, Roche Diagnostics, Indianapolis, IN) at 1:1,000 for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in SDS-PAGE loading buffer (50 mM TrisC1 pH 6.8, 2% SDS, 0.1% bromophenol blue, 10% glycerol, 4% β-mercaptoethanol, 1 mM PMSF, 1x Roche cOmplete protease inhibitor cocktail) and boiled for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... 5’-CAGACACGCAGACTCTTTC-3’) and 2.5 µl reverse primer (10 µM; 5’-CTAAAGATGTGTGTCTTCCTCA-3’) using the Expand Long Template PCR System (Roche). The PCR conditions were 35 cycles at 95°C for 30 sec ...
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bioRxiv - Molecular Biology 2021Quote: ... hygromycin B (Roche), fibronectin (sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... and B (Roche) overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... which were subsequently incubated with digestion buffer (IMDM supplemented with 2% FBS, 1 mg/mL Collagenase D [Roche], 2 U/mL DNase I [Life Technologies] and Dispase II [Roche]). The digested brain parenchyma ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cultured with 2 μg/ml of plate-bound anti-CD3 and anti-CD28 monoclonal antibodies (αCD3αCD28 stimulation) (mAbs) (eBioscience) and 25 U/ml of recombinant human interleukin-2 (IL-2; Roche Applied Science) at a concentration of 1.5-2 × 106 cells/ml in RPMI supplemented with 10% heat-inactivated Human Serum (HS ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM MgCl2; 2 mM EGTA pH 8.0; 0.1% Triton X-100; 0.1 mM PMSF; 1x Roche Complete protease inhibitors cocktail) supplemented with increasing NaCl concentrations (80-600 mM ...
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bioRxiv - Cancer Biology 2022Quote: NGS libraries were prepared from extracted gDNAs following a 2-step PCR protocol with 2 x KAPA Mastermix (KK2612, KAPA Biosystems). For spleen Tregs and CD4s ...
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bioRxiv - Cancer Biology 2024Quote: ... The aqueous phase from each MaXtract tube (∼400 µl) was then transferred to 1.5 ml tubes containing 2 µl of 2 µg/µl glycogen (Roche, Cat# 10901393001), 1 ml ethanol was added ...
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bioRxiv - Immunology 2024Quote: ... we quantified phosphorylated STAT5 (pSTAT5) levels following stimulation with recombinant human IL-2 (rhIL-2; Roche, Basel, CH-BS, Switzerland; [28]). Single-cell suspensions of splenocytes were obtained from 12-week-old NOD mice five weeks after in vivo treatment with IgG2a isotype control or anti-CD226 mAb ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated (T4 Ligase 5 U/µL, 9015-85-4, Roche) with the Xbal+SalI-HF-digested mTurquoise2-plasmid (#118617 ...
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bioRxiv - Microbiology 2021Quote: ... The infected PBMCs and CD4+ T cells were cultured at 37ºC in humidified air with 5% CO2 in the presence of 20 U/mL of IL-2 (Roche Diagnosis, Indianapolis, IN, USA), with various concentrations of S100A8 or S100A9 in RPMI-140 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... 3×10−4 M MTG and 300 μg/ml human transferrin (Roche, 10652202001)) in a triple vent petri 10 cm dishes (Thermo fisher ...
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bioRxiv - Genetics 2021Quote: ... with 3x protease inhibitors (cOmplete Protease Inhibitor Cocktail EDTA-free - 1 mM phenylmethylsulfonyl fluoride, 4 mM benzamidine, 2 μg/ml leupeptin, and 1 μg/ml pepstatin, Roche Diagnostics, cat. number 1187358001) and 3x phosphatase inhibitors (Millipore Inhibitor Cocktail Set ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS containing 0.5% Tween-20 samples were incubated with fluorescent labelled secondary antibodies containing 4′,6-diamidino-2-phenylindole (DAPI; Roche Cat# 10236276001, 1.0 µg/ml) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-CAA GTG CAA CAG TTT CTC ATT-3’/5’-TGT TTG ACT ACA CTC ACA CT-3’) using X-tremeGENE 9 DNA Transfection Reagent (Roche). 48 hours after transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... knee cartilage was harvested from postnatal day 3-5 mice and treated with 3 mg/ml collagenase D (11088866001, Roche) in DMEM (10569010 ...
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bioRxiv - Immunology 2021Quote: The measurement of anti SARS-CoV-2 neutralizing Abs was performed by electrochemiluminescence sandwich immunoassay (ECLIA) through Roche Elecsys Anti-SARS-CoV-2 S (Roche diagnostics, Switzerland). The neutralizing Ab were measured on a Cobas 601 modular analyzer (Roche diagnostics ...
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bioRxiv - Neuroscience 2022Quote: ... 1% sodium deoxycholate, 50 mM NaF, 2 mM EDTA, 2 mM DTT, 0.2 mM Na orthovanadate, 1 X Roche protease inhibitor #11836170001). Lysates were sonicated and centrifuged to remove debris ...
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bioRxiv - Molecular Biology 2022Quote: ... The ATP regeneration system (2 mM ATP, 2 mM phosphoenolpyruvate [PEP, Sigma-Aldrich, #P7127], 0.4 mM NADH [Roche, 10107735001], 1.67% (v/v) pyruvate kinase/lactate dehydrogenase mix [PK/LDH ...
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bioRxiv - Cell Biology 2023Quote: ... UGGT2-/- and UGGT1/2-/-cells were transfected with 1 µg Trop-2-pEYFP-N1 or Trop-2-Q118E-pEYFP-N1 plasmids using X-tremeGENE 9 DNA transfection reagent (Roche, Basel, Switzerland) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MBST + 10% HISS + 2% BMB for 2 h at room temperature and incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910) diluted in blocking buffer until signal development ...
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bioRxiv - Immunology 2020Quote: ... Cyclophilin B (control) and PPARγ siRNA (SMARTPool, Dharmarcon) were diluted in DOTAP (1,2-dioleoyl-3-trimethylammonium-propane; Roche Applied Sciences). The mix was gently mixed and incubated at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM EDTA and cOmplete mini-protease inhibitor (Roche)) mixed for 25 minutes at 4 °C to ensure complete lysis ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were preincubated in 2% blocking reagent (Roche, 11096176001) in MABT (100mM Maleic Acid ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM EDTA) with protease inhibitors (cOmplete Mini, Roche). Lysates were resolved on SDS-PAGE gels (Novex Tris-Glycine ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 200 U/ml of IL-2 (Roche) and 50 U/ml of penicillin/streptomycin (Gibco) ...
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bioRxiv - Zoology 2021Quote: ... 12.5 μl of 2× KAPA HiFi HotStart ReadyMix (Roche), 10 μl of 1μM forward and reverse primers and 2.5 μl of template DNA (5–20 ng/μl ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 mM MgCl2 with Protease Inhibitor Cocktail (Roche) and clarified by centrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM benzamidine and a protease inhibitor cocktail (Roche), and cleared by centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM 1,10-phenanthroline) containing protease inhibitor (Roche,#4693159001). The homogenates were centrifuged at 100,000 g ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 200 U/ml of IL-2 (Roche) and 50 U/ml of penicillin/streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... with 100 μl DNase I (2 mg/ml, Roche) in a 37 °C incubator shaking orbitally for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... and 2 mg/mL protease inhibitor mixture (Roche Diagnostics)) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μL of 25× protease inhibitor cocktail (Roche, #4693159001), 1 μL of 50 mM biotin ...
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bioRxiv - Cell Biology 2022Quote: ... and 2 mg/ml protease inhibitor mixture (Roche Diagnostics) and samples prepared ...
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bioRxiv - Microbiology 2020Quote: ... and recombinant interleukin-2 (20U/ml; Hoffmann-La Roche). Fresh medium containing IL-2 was added twice per week ...
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bioRxiv - Cell Biology 2021Quote: ... and protease inhibitors (2 complete EDTA-free pellets (Roche)/50 ml buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked in blocking buffer with 2% Blocking Reagent (Roche), 20% goat serum in MABT ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 mM PMSF and phosphatase inhibitor cocktail (PhosSTOP; Roche). For the analysis of RAN translation after cellular stress induction ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... and human recombinant IL-2 (30 U/ml, Roche) for 72 h.
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bioRxiv - Microbiology 2022Quote: ... using 2× Lightcycler 480 probes master (Roche, Indianapolis, IN) for IPO8 and Maxima SYBR Green/ROX qPCR Master Mix (2X ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT and 1 × cOmplete protease inhibitors (Roche) and sonicated for 15 minutes total time (10s on/20s off ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... 2% SDS) containing protease inhibitor cocktail (PIC 100X, Roche) and phosphatase inhibitors (Roche) ...
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bioRxiv - Immunology 2024Quote: ... collagenase P 2 mg/mL (Roche, Mannheim, Germany, #11213857001), then carefully removed ...