Labshake search
Citations for Roche :
3701 - 3750 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The genomic DNA was extracted from pools using High Pure Viral Nucleic Acid Kit (Roche, Germany) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR of the libraries was performed using an NGS Library Quantification Kit (Roche/Kapa Biosystems) and a QuantStudio 3 Real Time PCR Workstation (Thermo/ABI) ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR of the libraries was performed using an NGS Library Quantification Kit (Roche/Kapa Biosystems) and a QuantStudio 3 Real Time PCR Workstation (Thermo/ABI) ...
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bioRxiv - Molecular Biology 2024Quote: ... The amplified PCR products were purified using the High-Pure PCR Product Purification kit (Roche 11732676001) and sequenced on an Illumina NovaSeq 6000 platform ...
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bioRxiv - Molecular Biology 2024Quote: ... and were then quantified by qPCR using the KAPA Library Quantification Kit KK4835 (Ref. 07960204001, Roche). Library LME T0 was sequenced in an Illumina HiSeq2000 machine using a 125+125 nt paired end read format ...
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bioRxiv - Molecular Biology 2024Quote: ... To determine cycle number for amplification the KAPA SYBER FAST qPCR Master Mix Kit (KAPA Biosystems) is used in a qPCR ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed on 1.2 ng of gDNA using the FastStart Essential Green Master kit (Roche) and primers O1635 and O1636 for the dual-barcode sequence and O1583 and O1584 for pbp1a ...
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bioRxiv - Microbiology 2024Quote: ... The purified libraries were quantified using the KAPA library quantification kit (KAPA Biosystems, Wilmington, MA, USA). The CRISPRi-seq experiments in milk and BHI (without TMP-SMX ...
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bioRxiv - Genomics 2024Quote: ... the paired-end libraries was prepared by using KAPA LTP Library Preparation Kit (KK8232; Roche, Switzerland) without amplification ...
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bioRxiv - Genomics 2024Quote: ... the library was amplified using Kapa HiFi Uracil kit (KaPa Biosystems Pty, Cape Town, South Africa) under the following conditions ...
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bioRxiv - Bioengineering 2024Quote: ... The RNA was then converted to cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche). Quantitative PCR reactions were set up with the FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA processed at the RGC was prepared with a customized reagent kit from Kapa Biosystems and captured using the SeqCap VCRome 2 exome capture reagent or xGen lockdown probes ...
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bioRxiv - Genomics 2024Quote: ... A short-read whole genome sequencing library was prepared using the KAPA Hyper Prep Kit (Roche). A Hi-C library was prepared from kidney tissue using the Dovetail Omni-C Kit (Cantata Bio) ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified libraries were quantified using qPCR (KAPA Library Quantification Kit for Illumina® Platforms (Roche, 07960255001) and 1 x 75 bp sequencing performed using a high output flow-cell on a NextSeq 550 (Illumina).
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bioRxiv - Evolutionary Biology 2024Quote: ... real-time quantitative polymerase chain reaction (qPCR) (KAPA Library Quantification Kit; Kapa Biosystems, Wilmington, MA, USA), and small-scale sequencing (2x146bp ...
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bioRxiv - Bioengineering 2024Quote: Lactate dehydrogenase activity was measured using the Cytotoxicity Detection Kit PLUS (LDH) from Roche (Basel, Switzerland) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... Resulting libraries were quantified using the KAPA Library Quantification Kit specifically for Illumina platforms (KAPA Biosystems) and subjected to 42-bp paired-end sequencing on a NextSeq500 sequencer (Illumina) ...
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bioRxiv - Bioengineering 2024Quote: ... Subsequent steps involved mRNA enrichment and sequencing library preparation utilizing the KAPA mRNA HyperPrep Kit (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... PolyA- enriched RNA libraries were prepared with the KAPA Stranded mRNA Sample Preparation Kit (Kapa Biosystems). The RNAseq libraries were sequenced at 2x151 bp paired-end on NovaSeq 6000 at the Laboratory of Sequencing (Nencki Institute) ...
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bioRxiv - Cancer Biology 2024Quote: ... and the samples were amplified by PCR using the KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for a minimum of 16 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA Libraries were generated as described using Kapa Hyper Prep Kit (Roche and Illumina TruSeq system) and sequenced with NextSeq500 ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µm paraffin embedded human tissue slides were stained with OptiView DAB IHC Detection kit (Roche). The slides were permeabilized with 0.1 % Tween20 in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... then pooled libraries were quantified using the Illumina/Universal Library Quantification Kit (KAPA Biosystems, Cat. #KK4824). Libraries were sequenced on an entire lane of NextSeq 500 HO flow cell to generate paired-end 151 bp reads (300 cycles).
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bioRxiv - Developmental Biology 2024Quote: ... Library-prep with material on the beads was done using the Hyper Prep kit (Roche KK8502).
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bioRxiv - Developmental Biology 2024Quote: ... material was amplified by PCR using the Kapa polymerase from the Hyper Prep kit (Roche KK8502). Material from different samples was then combined and 1μg of pooled libraries was recaptured ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and further quantified by qPCR using the KAPA Library Quantification kit (Roche, Cape Town, South Africa). Barcoded cDNA libraries were subsequently pooled in equal molar ratios ...
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bioRxiv - Evolutionary Biology 2024Quote: ... samples were poly-A enriched and libraries were prepared using the Kapa HyperPrep Kit (Roche, #07962347001). RNA libraries were sequenced on a Novaseq2 with 100 bp paired-end reads ...
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bioRxiv - Developmental Biology 2024Quote: ... and Sox9 transcribed from linearized gene-specific probes (PCR DIG Probe Synthesis Kit; Roche, Cat. #11636090910). Primers for probe generation are listed in Extended Data Table 4 ...
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bioRxiv - Genomics 2024Quote: ... and end repair for all samples was performed using the KAPA HyperPrep kit (Roche, Indianapolis, IN). cfDNA inputs were normalized to 20 or 30ng ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was then amplified using an SYBR green RT-PCR kit (06924204001, Roche Products, Mumbai, India) for a 139 bp region in the Tat exon-1 using the primers- N1783 (5’- GGAATCATCCAGGAAGTCAGCCCGAAAC-3’ ...
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bioRxiv - Biophysics 2021Quote: Cholesterol concentrations were measured calorimetrically using a commercial kit (R-Biopharm and Roche Diagnostics, Cat. No. 10139050035) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labeled RNA probes were synthesized by in-vitro transcription using the DIG RNA labeling kit (Roche). In situ analysis was performed as described previously (Cecchetti et al. ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems, Wilmington, MA) according to the manufacturer’s protocol [39] ...
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bioRxiv - Immunology 2021Quote: ... Quantitative reverse transcription PCR (qRT-PCR) was performed using an SYBR FAST universal qPCR kit (KAPA Biosystems). Predesigned KiCqStart primers for DDX58 ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (Q-PCR) reactions were performed using LightCycler FastStart DNA MasterPlus SYBR Green I kit (Roche). For each primer set ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed using the KAPA SYBR FAST Universal qPCR Kit (KAPA BIOSYSTEMS) in a QuantStudio3 system (Life Technology) ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng of fragmented DNA was used for library preparation using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2021Quote: ... The library was quantified using the KAPA library quantification kit for Illumina sequencing platforms (KAPA Biosystems, KK4824) and 50bp single end reads were sequenced on a HiSeq2500 following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2019) via 15 rounds of high-fidelity PCR amplification (KAPA HotStart PCR Kit, KAPA Biosystems, Wilmington, Massachusetts). Each pooled ...
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bioRxiv - Evolutionary Biology 2021Quote: ... by using In Situ Cell Death Detection Kit (TMR red cat no. 1684795, Roche, Indianapolis, IN, USA) according to manufacturer’s recommendation ...
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Distal regulation, silencers and a shared combinatorial syntax are hallmarks of animal embryogenesisbioRxiv - Developmental Biology 2021Quote: ... The pool is assessed by qPCR using the KAPA Library Quantification Kit - Illumina/Universal (KAPA Biosystems, KK4824) combined with the Life Technologies Via 7 real-time PCR instrument ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Plant Biology 2022Quote: ... Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems) and average size fragment was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense probes were synthesized by in vitro transcription using a DIG RNA Labeling Kit (Roche). Following labeling ...
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bioRxiv - Physiology 2022Quote: ... using the LightCycler FastStart DNA Master plus SYBR Green I kit (catalog n° 03515869001, Roche, Basel, Switzerland). 40ng of reverse transcribed RNA was used as template for each reaction ...
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bioRxiv - Neuroscience 2021Quote: ... Library QC included size distribution check (BioAnalyser) and concentration determination with KAPA Library Quantification Kit (KK4857, Roche). Libraries were equimolar pooled and loaded on Illumina NovaSeq 6000 SP flowcell at 300 pM loading concentration with 1 % of PhiX by-mix ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was reverse transcribed with a Transcriptor First Strand cDNA Synthesis Kit (Roche Applied Sciences, Indianapolis, IN). Quantitative RT-PCR was performed using Applied Biosystems pre-designed TaqMan Gene Expression Assays for rats and TaqMan® Fast Advanced Master Mix ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA library was prepared for RNA-seq using KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems Inc.). The cDNA libraries were sequenced using Illumina HiSeq machine for paired-end reads using GENEWIZ (South Plainfield ...
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bioRxiv - Molecular Biology 2020Quote: ... The library pool was quantified via qPCR with a KAPA Library-Quant kit (Kapa Biosystems Cat#KK4824). Each sample was split in half and half were sequenced on an Illumina HiSeq 4000 system for single-end 100-bp reads ...
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bioRxiv - Neuroscience 2021Quote: ... TUNEL staining was performed according to manufacturer’s instructions (In situ cell death detection kit TMR red, Roche). For the positive control ...