Labshake search
Citations for Roche :
3601 - 3650 of 5049 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... the paired-end DNA library was constructed using a KAPA Hyper Prep kit (KAPA Biosystems, Wilmington, MA, USA). The fragmented DNA was end-repaired ...
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bioRxiv - Neuroscience 2021Quote: ... ATAC-seq library amplification was done using the KAPA SYBR FAST qPCR Master Mix (2X) Kit (Kapa Biosystems) and 1.25 μM indexed primers using the following PCR conditions ...
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bioRxiv - Epidemiology 2021Quote: ... Resulting cDNA was used for quantitative real-time PCR (qPCR) analysis with a SYBR Green PCR kit (Roche). β-Actin was used as reference gene ...
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bioRxiv - Developmental Biology 2020Quote: ... Digoxigenin (DIG) and fluorescein labeled (FITC) probes were synthesized by using DIG and FITC RNA labelling kit (Roche). Primer sequences are listed in Table S1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared from 250 ng of total RNA using the KAPA stranded mRNA-seq kit (Roche, KK8421) according to the manufacturer’s specifications ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries from 5-10 ng of ChIP DNA was prepared using the KAPA HTP Library Preparation Kit (Roche) by the Next-Generation Sequencing Core at UT Southwestern ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was extracted from cells using High pure RNA isolation kit from Roche (Ref. 11828665001, lot. 38800800) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... was used to remove excess antibody and amplified C-circles were detected with CDP-Star® kit (Roche). Membranes were imaged with the Odyssey® Fc Imager (LI-COR ...
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bioRxiv - Genomics 2020Quote: Pre-capture libraries were prepared with standard library preparation protocols using the KAPA Hyper kit from Kapa Biosystems and then pooled at equal volume and sequenced on the Illumina platform at low depth to determine exact relative abundances ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from 500 uL of nasal wash fluid using the high Pure RNA Tissue Kit (Roche) and DNA was amplified in the Light Cycler (Roche ...
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bioRxiv - Microbiology 2021Quote: The RNA was subjected to reverse transcription and second strand synthesis using the cDNA synthesis system kit (Roche). The acid nucleic quantification (RNA and cDNA ...
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bioRxiv - Immunology 2020Quote: ... 120 ng of RNA were used in the Transcriptor First Strand cDNA synthesis kit (Roche Diagnostics, Laval, QC), and 0.1 μl to 0.2 μl of cDNA generated were analyzed by SYBR Green-based real time PCR (real time-PCR ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time PCR reactions were performed with SYBR Green I Master Kit and LightCycler 480 system (Roche). mRNA levels of Col4a1 were normalized to the housekeeping gene Actb or Rpl32 ...
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bioRxiv - Immunology 2021Quote: ... A pre-amplification step of 9 PCR cycles was performed using the Kapa HiFI Hoststart kit (Kapa Biosystems). The PCR product was purified using AmpureXP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA from LCLs was reverse transcribed to cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche, Cat. # 04896866001). RT-qPCR was performed in triplicate using Fast SYBR Green Master Mix (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... followed by cDNA synthesis using 100 ng RNA with the First Strand cDNA synthesis kit (Roche Applied Science) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Library preparation was performed according to the manufacturer’s protocol using a Kapa Hyper Prep Kit (Kapa Biosystems, KK8501) and barcoded independently with unique dual indexes (IDT for Illumina ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A total of 100 ng RNA was subsequently reverse transcribed using the Transcriptor First Strand cDNASynthesis Kit (Roche) at 55 °C for cDNA synthesis ...
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bioRxiv - Developmental Biology 2022Quote: Digoxigenin-labeled RNA probes were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics, Basel, Switzerland). In situ hybridizations were performed on 16μm cryosections that were rehydrated in 1x PBS for 5min ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were stained using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL apoptosis assay kit, Roche, American), and the cells were viewed under a fluorescence microscope.
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bioRxiv - Immunology 2023Quote: ... RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche) using 250 ng of total RNAs as input ...
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bioRxiv - Neuroscience 2023Quote: ... remaining tissue was lysed and gDNA amplified by means of PCR (KAPA2G Fast HotStart PCR kit, KAPA Biosystems) as described above ...
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bioRxiv - Genomics 2023Quote: ... The end-repair and A-tailing (ERAT) enzyme mix with a purple cap (KAPA HyperPlus kit, Roche v5.19) needs to be used for this protocol since the ERAT enzyme mix with an orange cap in the KAPA HyperPlus kit ...
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bioRxiv - Neuroscience 2023Quote: ... Salivary cortisol concentrations were measured with electro-chemiluminescence-assay (ECLIA) kit (Cobas®, Roche Diagnostics GmbH, Mannheim, Germany). Samples (IDs ...
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from tumoroids was done following the manufacturer protocol using the High Pure RNA Isolation Kit (Roche).
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bioRxiv - Developmental Biology 2023Quote: Digoxigenin-labeled cRNA probes were prepared via in vitro transcription (DIG RNA labeling kit; Roche Diagnostics, Basel, Switzerland) from the Prokr2 template available from Genepaint (https://gp3.mpg.de/viewer/setInfo/MH1790/12) ...
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bioRxiv - Cancer Biology 2023Quote: Proliferation was measured by using 5-bromo-2’-deoxy-uridine (BrdU) labelling and detection kit (Roche, BSL, CH) as described earlier (25) ...
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bioRxiv - Cancer Biology 2023Quote: Low-pass WGS libraries were created using the Kapa Biosystems Hyper Prep kit (KAPA Biosystems, Capetown, South Africa) using 11.8 ng/ml of G4_1 ...
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bioRxiv - Microbiology 2023Quote: The RNA of all cell culture supernatants was extracted using High Pure Viral RNA kit (Roche, REF.11858882001). Viral RNA quantification in each sample was performed by quantitative reverse transcription PCR (RT-qPCR ...
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bioRxiv - Plant Biology 2023Quote: ... Hybridization and probe detection was performed using a DIG Luminescent Detection Kit for Nucleic Acids (Roche Diagnostics, UK) as per the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... 2 ul of each sample were used for library quantification using the KAPA Library Quantification Kit (Roche #KK4923). The rest of the DNA eluate (20ul ...
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bioRxiv - Immunology 2023Quote: ... RNA from mouse brainstem or primary cell cultures was isolated using the High Pure RNA Isolation Kit (Roche). HiPSC-derived cells were lysed in RLT Plus buffer (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... quantitative PCR (RT-qPCR) with the SensiFAST SYBR No-ROX One-Step Kit in a LightCycler 96 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 500 ng of RNA was prepared for sequencing using the KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems). Libraries were sequenced on the NextSeq 500 (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... RNA was re-extracted from original material using the High Pure RNA Isolation Kit (Roche Diagnostics GmbH Mannheim). A multi segment RT-PCR amplification was performed using the Superscript III high-fidelity RT-PCR Kit (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Libraries were quantified by qPCR using the Library Quantification Kit for Illumina sequencing platforms (KAPA Biosystems, cat#KK4824) using 7900HT Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... 100 ng total RNA was reverse transcribed into cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... We quantified libraries by qPCR using the Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, cat#KK4824) and the 7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... The optimal numbers of PCR cycles were pre-determined using the KAPA Real-Time Library Amplification Kit (Roche) as described previously (Tanegashima et al ...
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bioRxiv - Immunology 2023Quote: ... All libraries were quantified via Agilent 2100 hsDNA Bioanalyzer and KAPA library quantification kit (Roche, Cat. No. 0796014001.) Gene expression libraries were sequenced at a targeted depth of 25,000 reads per cells ...
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bioRxiv - Genomics 2022Quote: ... The short reads were obtained by preparing Illumina PCR free libraries using the Kapa Hyper Prep Kit (Roche). Briefly ...
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bioRxiv - Cell Biology 2022Quote: DNA sequencing by NGS consisted of two PCR amplification steps with KAPA HiFi HotStart ReadyMix PCR Kit (Roche). The first step (18 cycles ...
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bioRxiv - Immunology 2022Quote: ... RNA libraries were generated using the KAPA Stranded mRNA-Sequencing Kit with KAPA mRNA Capture Beads (Roche #07962207001). Samples were enriched for poly(A)+ mRNA strands using mRNA Capture Beads ...
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bioRxiv - Microbiology 2022Quote: ... HBsAg titers were quantified by use of Elecsys HBsAg II Quant reagent kits (Roche Diagnostics, Indianapolis, IN, USA). HBV DNA levels were quantified by use of the Abbott RealTime HBV assay (Abbott Laboratories ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the ligated products were amplified using Kapa Hifi HotStart NGS library Amplification kit (Kapa Biosystems, Wilmington, MA, USA), followed by 0.6x AMPure XP purification.
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bioRxiv - Cancer Biology 2022Quote: ... Library preparation was performed with 1 μg of DNA and KAPA HTP Library Preparation Kit (KAPA Biosystems, #KK8234). Resulting reads (50 base single-end reads ...
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bioRxiv - Cancer Biology 2024Quote: ... Full-length RNA was made into libraries using the KAPA stranded mRNA library prep kit (Roche, Nutley, NJ) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Cell metabolic viability was measured by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Roche). After treatments ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was amplified using the 2X KAPA Taq ReadyMix PCR Kit (KAPA Biosystems, now Roche Sequencing, Indianapolis, IN) for 35 cycles using an annealing temperature of 64°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Probes were transcribed with TranscriptAid T7 High Yield Transcription kit (Thermo, KO441) using DIG-Label UTP (Roche,11127073910).