Labshake search
Citations for Roche :
3851 - 3900 of 5049 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The molar concentration of the pool was measured using the KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA) and the pool was sequenced on one lane of the Illumina HiSeq 4000 sequencer with a 75 bp Paired-End SBS chemistry (Illumina).
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bioRxiv - Genetics 2023Quote: ... Real time PCR was performed on the cDNA using KAPA SYBR FAST qPCR Master Mix (2X) kit (Roche, #KK4618) with intron spanning primers in a QuantStudio 3 Real-time PCR system ...
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bioRxiv - Molecular Biology 2023Quote: ... Highly sensitive DNAprobes labelled with digoxigenin-dUTP were generated using the PCR digoxigenin probe synthesis kit (Roche Applied Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2023Quote: ... Libraries were quantified by qPCR using the Library Quantification Kit for Illumina sequencing platforms (cat#: KK4824, KAPA Biosystems, Boston), using 7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 6ng of ChIP DNA was processed for library preparation for Next Generation Sequencing by using Kapa kit (Roche) following the manufacturer’s instruction with one modification ...
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bioRxiv - Neuroscience 2023Quote: ... and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit; Roche Tissue Diagnostics). Sections were treated with Discovery CC1 cell conditioning solution at 95°C for 60 min followed by a low concentration of protease (Protease 3 ...
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bioRxiv - Microbiology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Genomics 2023Quote: TP pre-capture libraries were prepared as previously described [15] using the KAPA HyperPlus kit with UDI adapters (Roche). 17.5µL DNA was used as input for fragmentation for eight minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... B-16 cells were collected after 48 hours of transfection and RNA was extracted using (Roche, RNA isolation Kit). Total RNA was quantified on a nanodrop (Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... Poly(A)-enriched next-generation sequencing library construction was performed using the KAPA mRNA Hyper Prep Kit (KAPA Biosystems) with 100 ng of input total RNA and 15 amplification cycles according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Quantitative PCR was carried out on standards and test samples using the LightCycler 480 Probes Master kit (Roche, 04707494001) with inverted terminal repeat probe and primer sequences indicated in Table S4.
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription (IVT) was performed using T7 mMassage mMachine kit (invitrogen) and purified using RNA purification column (Roche). MCU-IVT was injected at single cell stage and embryos were cultured in E3 water ...
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bioRxiv - Bioengineering 2023Quote: ... of the VH gene was subjected to second-strand synthesis in a 25 μl reaction volume using IGHV gene-specific primers (primer No. 23–32, 7.5 μl) and a KAPA Biosystems kit (Roche). The reaction conditions were as follows ...
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bioRxiv - Neuroscience 2022Quote: ... and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit; Roche Tissue Diagnostics). For haematoxylin and eosin (H&E ...
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bioRxiv - Physiology 2022Quote: Both ATP release and intracellular ATP from isolated mesenteric arteries were performed using a ATP Bioluminescence HSII kit (Roche) kit as previously described.3
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR amplification was performed in two technical replicates using KAPA Hifi Hotstart PCR kit (Kapa Biosystems, MA, USA) via a Bio-Rad CFX96 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... We prepared RNA libraries using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adaptors (KAPA Biosystems; Massachusetts, USA), with 200 ng of RNA as input ...
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bioRxiv - Molecular Biology 2024Quote: ... The preparation of next generation sequencing libraries was done using the KAPA HyperPrep ChIP Library kit (Roche Sequencing solutions) at the molecular biology and functional genomics platform of the Institut de Recherche Clinique de Montréal (IRCM) ...
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bioRxiv - Genetics 2023Quote: ... Sequencing libraries were prepared using 100 ng fragmented DNA with the KAPA HyperPrep Library Prep Kit (Roche, Indianapolis, IN) and NEXTFLEX UDI barcodes (PerkinElmer ...
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bioRxiv - Microbiology 2024Quote: ... six reverse primers (10 μM) and Robust DNA polymerase from the KAPA2G Robust PCR Kit (Kapa Biosystems, Wilmington, MA). Cycling conditions were ...
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bioRxiv - Neuroscience 2024Quote: ... 5-diphenyl tetrazolium bromide (MTT) in accordance with the manufacturer’s recommendations (Cell Proliferation Kit I; Roche Diagnostics, Basel, Switzerland). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... membrane hybridization and detection were performed using DIG-High Prime DNA Labelling and Detection Starter Kit II (Roche, #11585614910) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche) with random primer ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... cDNA was synthesized with 200ng of the stored RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, 04897030001) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche, #11175025910). Probes were then cleaned with MegaClear Kit (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated from cells and reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche). Gene expression was quantified using FastStart Roche SybrGreen (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... For library preparation the Kapa LTP Library Preparation Kit for Illumina Platforms was used (Kapa Biosystems, Part Number KK8232). The cDNA was filtered into the End Repair step of the method and the library preparation was followed as per the protocol through to Adapter Ligation ...
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bioRxiv - Molecular Biology 2024Quote: Analysis of ChIP-purified DNA by quantitative PCR (qPCR) was performed using KAPA SYBR Fast qPCR kit (KAPA Biosystems) and StepOnePlus real-time PCR systems (Life Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... Library quality control and quantification were performed using a KAPA Library Quantification kit for Illumina platforms (Kapa Biosystems, KK4873) and a 2100 Bioanalyzer High Sensitivity DNA kit (Agilent ...
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bioRxiv - Systems Biology 2023Quote: ... RNA libraries were prepared for paired-end sequencing using the KAPA Stranded RNA-Seq Library Preparation kit (Kapa Biosystems). Libraries were sequenced on a NextSeq system (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 20 x 106 cells were used for isolating genomic DNA with High Pure PCR Template Preparation Kit (Roche) as described by the manufacturer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche, Basel, Switzerland) with adaptor ligation and adaptor fill-in reactions based on Meyer and Kircher (2010) ...
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bioRxiv - Genomics 2023Quote: Carver Biotechnology Center at the University of Illinois at Urbana-Champaign with the Hyper Library Construction Kit (Kapa Biosystems) to obtain an average library fragment size of 500bp ...
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bioRxiv - Developmental Biology 2023Quote: ... The molarity of adaptor-modified molecules was defined by a qPCR with a KAPA Library Quantification kit (Kapa Biosystems). Individual libraries were normalized to 10nM ...
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bioRxiv - Microbiology 2023Quote: ... The PCR DIG Probe Synthesis kit for probe synthesis and the CSPD luminescent detection kit for visualization of the bound probe were used according to the manufacturer’s instructions (Roche Molecular Biochemicals ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Microbiology 2023Quote: ... we performed target-capture using a custom-designed probe kit with a SeqCap EZ Workflow (Roche, Indianapolis, IN, USA), which was designed to capture the entire nuclear genome of S ...
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bioRxiv - Immunology 2023Quote: ... Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete, Universal) (Kapa Biosystems, Woburn, MA). Pooled libraries were sequenced on an Illumina NovaSeq 6000 using 150bp PE reads (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from A549 cells grown in 6-well plates was done using High Pure RNA isolation kit (Roche). Transcriptor First strand kit (Roche ...
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bioRxiv - Cell Biology 2023Quote: MTT activity was determined using the Cell Proliferation Kit I according to the recommendations of the manufacturer (Roche, Spain). Optical density was determined at 575[nm with a reference wavelength of 690[nm using a Varioskan Flash spectrophotometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of each captured library pool was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (Roche) to produce cluster counts appropriate for the Illumina NovaSeq-6000 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... The ribodepletion method of library preparation for RNA sequencing was performed using the Total-RNASeq RiboErase Kit (KAPA Biosystems). All RNA samples were loaded two separate 2-lane flow cells and sequenced using an Illumina HiSeq 2500 system generating 51 bp paired-end reads ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (1Lμg) was used to generate RNA-seq library with KAPA HyperPrep RNA Kits with RiboErase (Roche KK8560) using 8 cycles of PCR amplification ...
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bioRxiv - Microbiology 2023Quote: Sequencing was done using Illumina HiSeq X with sequencing shotgun library preparation using the KAPA HyperPrep Kit (Kapa Biosystems) following the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and long-read (Nanopore) platforms: the Illumina library was prepared using the Kapa HyperPrep kit (Kapa Biosystems, Wilmington, MA). A-tailing ...
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bioRxiv - Developmental Biology 2023Quote: ... We synthesized digoxigenin (DIG)-labeled sense and antisense RNA probes using a DIG RNA Labeling Kit (Roche, Basel, Switzerland) after digestion with the appropriate restriction enzymes BamHⅠ-HF ...
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bioRxiv - Developmental Biology 2023Quote: Genotyping of ESCs and mouse tails were performed by PCR using KAPA Mouse Genotyping Kit (Roche, Cat. No. KK7302). Specific protocol followed the reagent instructions ...