Labshake search
Citations for Roche :
3601 - 3650 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... 2.5 μL of 100 μM sub-barcode primer and 25 μL of KAPA HiFi Hotstart master mix (Roche) was added to each well and the plate was thermocycled (95 °C 3 min. ...
-
bioRxiv - Plant Biology 2022Quote: ... o,1% SDS and 5mM DTT) supplemented with a 1:100 dilution of plant protease inhibitor cocktail (Roche) was added to the ground samples and centrifuged 20 minutes at 6,000 x g at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1% Triton X-100, 50 mM Tris pH 7.5, 0.1% SDS, 0.5% sodium deoxycholate, and protease inhibitors [Roche 11836170001]). After 20 min incubation on ice ...
-
bioRxiv - Neuroscience 2022Quote: Brain sections were washed three times then permeabilized in TBS with 0.2% Triton-X 100 (TBST; Roche, Switzerland) at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: ... in Buffer D (20mM HEPES pH 7.9, 150mM KCl, 20% glycerol, 0.2mM EDTA, 0.2% Triton X-100 and 1x complete Roche inhibitor) overnight at 4°C ...
-
bioRxiv - Genomics 2024Quote: ... 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium Deoxycholate, 0.1% SDS, 1× Protease Inhibitor cocktail, Roche) and incubated at 4 °C for 1 hour with rotation ...
-
bioRxiv - Cell Biology 2023Quote: DIV 12-14 cortical neurons were scraped from the plate in lysis buffer (PBS pH 7.5, 0.5% v/v Triton X-100 supplemented with protease inhibitors (EDTA-free Complete; Roche), kept on ice for 15 min and then centrifuged at 15,000 x g for 10 min ...
-
bioRxiv - Cell Biology 2024Quote: ... in Buffer D (20 mM HEPES pH 7.9, 150 mM KCl, 20% glycerol, 0.2 mM EDTA, 0.2% Triton X-100 and complete Roche inhibitor) overnight at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: Dissected embryonic brain structures were lysed in RIPA buffer (Tris HCl 1M, pH 7.7; NaCl 5M; EDTA 0.5M; Triton X-100) supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100) complemented with final concentration of 1X EDTA-free Complete protease inhibitor cocktail (34044100, Roche) and 1 mM PMSF (78830 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were then resuspended in sonication buffer (50 mM Tris-HCl pH 8.1; 10 mm EDTA; 1% Triton-X; 0,1% deoxycholate sodium; 100 mM NaCl, 1mM PMSF, Proteinase inhibitor Roche) and sonicated 6 times with 2 min cycles (Branson Sonifier 250 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and then lysed and harvested in ChIP lysis buffer (50 mM Tris-HCl pH 8.1; 0.5% SDS; 10 mm EDTA;100 mM NaCl, 1mM PMSF, Proteinase inhibitor Roche) by centrifugation for 6 min at 2,000 × g ...
-
bioRxiv - Plant Biology 2023Quote: ... 50 grams of Arabidopsis leaves were homogenized for 1 min in 2 volumes of resuspension buffer (100 mM MES, pH 6.4, 3mM EDTA, 0.5 mM MgCl2, 1mM EGTA, protease inhibitors (Roche)) using a Waring Blender ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1% Triton X-100) supplemented with 1X complete protease inhibitor cocktail and 1X complete phosphatase inhibitor cocktail (Roche) using a Precellys Evolution homogenizer (Bertin Technologies) ...
-
bioRxiv - Genetics 2023Quote: ... 150 mM NaCl, 2.5 mM MgCl2, 250 mM sucrose, 0.05% NP40, 0.5% Triton X-100, 1x Roche-Complete). Samples were cleared by centrifugation at 10000 × g at 4°C for 10 min ...
-
bioRxiv - Cell Biology 2023Quote: ... and lysed in lysis buffer (50mM Tris, pH 7.4, 500mM NaCl, 0.4% SDS, 2% Triton-X-100, 1mM DTT, 1x complete protease inhibitor [Roche]). Lysates were sonicated twice for 1 minute at 30% duty cycle at an output level of 4 ...
-
ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... 50 mM Tris-HCl pH8.0, 0.3 M NaCl, Triton X-100 1%, EDTA-free Complete Proteases inhibitor cocktail from Roche and 250U/ml of benzonase endonuclease from Sigma) ...
-
bioRxiv - Immunology 2023Quote: ... HEK293T cells were lysed in LUMIER lysis buffer (50 mM Hepes-KOH pH 7.9, 150 mM NaCl, 2 mM EDTA, 0.5% Triton X-100, 5% glycerol and Roche cOmplete™ Mini protease Inhibitor Cocktail ...
-
bioRxiv - Cell Biology 2023Quote: ... spun at 20,000 × g for 5 min was mixed with 75 μl of RNA pulldown buffer (1× PBS, 0.1% Triton X-100, 0.6 mM PMSF, and complete EDTA-free protease inhibitor cocktail [Roche]) (final protein concentration ...
-
bioRxiv - Cell Biology 2023Quote: ... The NEs were diluted with IP buffer (1× PBS, 0.1% Triton X-100, 0.6 mM PMSF, and cOmplete EDTA-free protease inhibitor cocktail [Roche]) (protein concentration ...
-
bioRxiv - Biochemistry 2023Quote: ... pH 7.9; 5 mM MgCl2; 0.2% Triton X-100; 20% glycerol; 300 mM NaCl and freshly added ROCHE cOmplete protease inhibitor cocktail at 0.01 tablet/mL) ...
-
bioRxiv - Plant Biology 2023Quote: ... and 0.1% [v/v] Triton X-100) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, catalog no. 11873580001), 1 mM PMSF ...
-
bioRxiv - Physiology 2024Quote: ... 1% Triton X-100, 0.1% sodium deoxycholate, 1mM EDTA, 5mM MgCl2, 1mM DTT, 0.5mM PMSF, 10mM NaF, Roche PhoSTOP ...
-
bioRxiv - Physiology 2024Quote: ... 5 mM EDTA, 2% Triton-X-100, 0.2 mM HDSF, pH 7.4, and protease inhibitor cocktail from Roche). Following sonication on ice ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were thawed on ice and disrupted in lysis buffer (100 mM Tris-HCl pH 7.4, 0.5% Triton X-100, 150 mM NaCl, 13% w/v glycerol, protease inhibitor tablet, Roche) by bead beating (mini bead beater-16 ...
-
bioRxiv - Biochemistry 2024Quote: ... Frozen cell pellets were resuspended in Lysis Buffer (50 mM HEPES [pH 7.5], 100 mM NaCl, 1 mM NaF, 0.1% βME with Roche EDTA-free protease inhibitor tablets ...
-
bioRxiv - Neuroscience 2024Quote: ... cells from one 10 cm dish were lysed with 400 µl lysis buffer (50 mM HEPES pH 7.4, 300 mM NaCl, 0.5 % Triton X-100, complete protease inhibitor cocktail [Roche]). The lysate was centrifuged at 13.000 x g for 15 min and the supernatant (SN ...
-
bioRxiv - Biochemistry 2024Quote: ... cremoris samples were resuspended in 100 mM Tris-HCl (pH 8.5) with a protease inhibitor cocktail tablet (Roche) and 2% sodium dodecyl sulfate ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Blocking was performed in MAB blocking buffer (100 mM Maleic acid, 150 mM NaCl, 2% Blocking reagent [Roche, 1096176] ...
-
bioRxiv - Plant Biology 2019Quote: ... 4 °C) the cleared supernatants were diluted 1 time using water containing 1x cOmplete ™ EDTA-free protease inhibitor (Roche). Protein extract was incubated overnight with anti-HA antibodies (Roche) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and cell suspensions incubated for 10 min at 4°C in SDS lysis buffer containing protease inhibitors (Complete protease inhibitor cocktail, Roche). The remaining procedures were performed as described in previous reports (Carvajal-Gonzalez et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... bovine serum albumin in TBS-T and incubated for 16 h at 4°C: mouse anti-GFP (1:1000) (Roche), sheep anti-CK1α (1:100 ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein pellet was resuspended in 100ul of Lysis Buffer (150mM NaCl, 4%SDS, 50mM TrisHCl pH 8.0, 10mM EDTA, complete protease and phosphatase inhibitor cocktails-Roche diagnostics).
-
bioRxiv - Genetics 2021Quote: ... and Tvrm323 mice (n = 4) eyes at one month of age were dissected in ice- cold PBS with proteinase inhibitor (Roche) and snap frozen in eppendorf tubes on dry ice.
-
bioRxiv - Microbiology 2021Quote: ... and incubated with blocking buffer (4 % skim milk in 1 × PBS) followed by incubation with the primary antibody: (monoclonal anti-GFP mice 1:5000 (Roche), rabbit anti-GFP 1:10000 ...
-
bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC ...
-
bioRxiv - Developmental Biology 2021Quote: ... Gene expression signals were visualized using nitroblue tetrazolium/5-bromo-4-chloro-3-indolylphosphate solutions using a standard method (Roche). Whole-mount and sectioned specimens of Ciona were observed using an SZX12 stereo microscope (Olympus ...
-
bioRxiv - Developmental Biology 2020Quote: ... followed by visualization with nitro blue tetrazolium and 5-bromo-4-chloro-3-indolylphosphate (NBT/BCIP) (Roche Diagnostics, Basel, Switzerland). Embryos were imaged using a Leica M205 FA epifluorescence microscope (Leica ...
-
bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4°C with anti-Digoxigenin antibody coupled to alkaline phosphatase (Roche, 11207733910, used at 1/4000). The last day ...
-
bioRxiv - Developmental Biology 2020Quote: ... in PBST supplemented with sheep serum and BSA before being incubated overnight at 4°C with an anti-digoxygenin antibody (Roche). After removal of the antibody ...
-
bioRxiv - Developmental Biology 2020Quote: ... The embryos were subsequently incubated in the dark on a slow rocker in dilutions of Nitro-blue tetrazolium/5-bromo-4-chloro-3-inodyl phosphate (NBT/BCIP; Roche) in TBST ...
-
bioRxiv - Developmental Biology 2020Quote: To prepare nuclear extracts all procedures were done at 4°C and buffers supplemented with 1x protease inhibitors (Roche, 4693132001), 15 μM MG-132 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2022Quote: Total RNA from 4 replicates of BCBL-1 WT clone B4 and 57KO clone #6 cells were isolated by TriPure Reagent (Roche) 24 h after induction with VA ...
-
bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified for 30min at 15,000xg and the supernatant was immunoprecipitated for 2h at 4°C with Dynabeads-protein G covalently coupled to the rat anti-HA antibody (Roche). The beads were washed 3×5 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... and the soluble fraction was incubated for 1 h at 4°C with beads crosslinked to mouse anti-GFP antibody (Roche). Resulting Immunoprecipitates were washed three times with lysis buffer and protein eluted with RapiGest surfactant (Waters ...
-
bioRxiv - Genomics 2020Quote: ... and a potential large inversion on chromosome 4 (Supplementary Table 11) were labeled with digoxigenin-11-dUTP (Roche, Indianapolis, IN), biotin-16-dUTP (Roche) ...
-
bioRxiv - Genomics 2020Quote: ... Real-time quantitative PCR reactions were set up in triplicate with 2 μL of cDNA (1:4 dilution) per reaction using the FastStart Universal SYBR Green Master mix (Rox) (Roche) and run on a 7500 Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Immunology 2019Quote: ... Tissue sections were placed on charged glass slides and fixed with 4% PFA in 2xPBS with 2x Complete protease inhibitor (Roche) for 10 min ...
-
bioRxiv - Developmental Biology 2019Quote: ... probes were synthesized at 37°C for 2-4 hrs in digoxigenin-synthesis reaction mixture with T3 RNA polymerase (Roche). After synthesis ...
-
bioRxiv - Molecular Biology 2019Quote: ... Following the preparation of plates an incubation at 4°C for at least 60 min was performed before the plates were analyzed on a LightCycler 480 II (Roche) using 45 cycles and a temperature profile recommended by the manufacturer for miRCURY SYBR® Green mastermix ...