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Citations for Roche :
3551 - 3600 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 1% Triton X-100, 50 mM Tris pH 7.5, 0.1% SDS, 0.5% sodium deoxycholate, and protease inhibitors [Roche 11836170001]) for 20 min on ice ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All aliquots were then treated with 0.25 μL 100 mM PMSF and 2.8 μL 5x storage buffer (5x Roche cOmplete™ Mini proteinase inhibitor cocktail ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected hippocampi were dissolved in lysis buffer (1M Tris-Cl, pH 7.5; 6M NaCl; 10% SDS; 0.5M EDTA; Triton-X 100; Phosphatase Inhibitor #3, Roche, #05-892-970-001 ...
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bioRxiv - Developmental Biology 2019Quote: ... 1.5% Triton X-100, 1% IGEPAL, 0.5% Sodium Deoxycholate, 0.1% SDS, 1 mM DTT, 1x Complete protease inhibitor [Roche]) for 30 minutes at 4°C under rotation ...
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bioRxiv - Microbiology 2019Quote: ... was brought to 500 μL with ChIP dilution buffer (16.7 mM Tris-HCl pH 8.0, 1.1% Triton X-100, 1.2 mM EDTA, 167 mM NaCl and cOmplete EDTA-free Protease Inhibitors [Roche]) and incubated with 10 μg mouse monoclonal anti-RNAPII (BioLegend ...
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bioRxiv - Genomics 2019Quote: ... 1% Triton X-100, 50 mM Tris pH 7.5, 0.1% SDS, 0.5% sodium-deoxycholate, and protease inhibitors [Roche 11836170001]). Lysates were passed 10 times through a 28.5 gauge needle and cleared by centrifugation at 20,000xg for 20 min at 4°C ...
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bioRxiv - Genetics 2019Quote: ... After 3h at 4°C slides were washed and blocked with 0.5x blocking buffer (1.5% BSA, 5% goat serum, 0.05% Triton X-100) containing protease inhibitors (Roche 1836153) for 1h at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... hearts were enzymatically digested by perfusing with the same solution plus 100 μM Ca2+ and Liberase TM (Roche, final 0.25 collagenase Wunsch units/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... 110 mM KOAc, 0.5% Triton X-100, 0.1% Tween 20, 10 mM MnCl2, 1x proteinase inhibitors (complete, EDTA-free (Roche)) ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.9; 10 mM KCl; 0.1% Triton X-100; 20% Glycerol; 0.5mM spermidine; 1x complete protease inhibitor [Roche # 11836170001]), collecting by centrifugation (600 g ...
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bioRxiv - Biochemistry 2021Quote: Rat total RNA was prepared from 100 mg of kidneys with the use of TriPure reagent (Roche, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the reaction product was developed using NBT/BCIP solution diluted in pre-staining buffer (1:100; Roche, Switzerland) overnight at RT ...
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bioRxiv - Molecular Biology 2021Quote: Total proteins from HEK293T cells were extracted using lysis buffer (150mM NaCl, 50 mM Tris-HCl, pH7.4, 0.5% Triton X-100) adding protease inhibitor cocktail (#11836153001, Roche). Prepared protein samples were separated by 12% or 15% SDS-PAGE and subsequently transferred onto a 0.2μm PVDF membrane (Pall) ...
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bioRxiv - Cell Biology 2021Quote: ... 350 mM NaCl, 2 mM MgOAc, 1 mM CaCl2, 15% glycerol, 0.1% Triton X-100, 0.05% 2-ME, 1x Roche protease inhibitors ...
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bioRxiv - Microbiology 2021Quote: Purified BUNV RNPs were diluted in TNE buffer (100 mM Tris-HCl, 200 mM NaCl, 0.1 mM EDTA, pH 7.4, supplemented with protease inhibitors (Roche)) and mixed with indicated amounts of RNase A to a final volume of 20 μL ...
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bioRxiv - Microbiology 2021Quote: ... Pellets were resuspended in 1 ml buffer (50 mM Tris, pH 8.0, 10 mM DTT, 0.1% SDS, 100 μM PMSF, phos-stop tablet [Roche]), and cells were lysed by 2 minutes of vortexing with zirconia beads (BioSpec Products) ...
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bioRxiv - Neuroscience 2019Quote: Retina sections were washed three times then permeabilized in PBS with 0.3% Triton-X 100 (PBST; Roche, Switzerland) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were harvested and lysed in IP buffer (1 x PBS, 3 mM KCl, 2.5 mM MgCl2, 0,5 % Triton X-100 and protease inhibitors from Roche). 35 μl of this lysate was loaded onto an SDS-gel (lysate lanes) ...
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bioRxiv - Molecular Biology 2022Quote: ... 20mM MOPS) pH 7.0 plus 0.1% Triton X-100 and 1x cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany) on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 mM DTT and 0.5% Triton X-100 (pH 7.5) supplemented with complete protease inhibitor cocktail tablets (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells from one 15 cm dish were lysed in 500 μl lysis buffer (50 mM HEPES, 300 mM NaCl, 0.5% Triton X-100, pH 7.4) supplemented with protease inhibitors (Roche). After clearing debris by centrifugation ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was homogenized in 100 μL of ice-cold 0.1 M PBS (pH 7.4) supplemented with a protease inhibitor cocktail (Roche). The homogenate was divided into two fractions for further total mRNA and protein extraction ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using SYBR Green qPCR Master Mix (TOYOBO, QST-100) in the LightCycler480 system (Roche). The constitutively expressed tubulin gene (MGG_00604 ...
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bioRxiv - Genetics 2022Quote: ... pre-equilibrated with Buffer D (20mM HEPES pH 7.9, 150mM KCl, 20% glycerol, 0.2mM EDTA, 0.2% Triton X-100 and complete Roche inhibitor) and carried out on a rotating wheel over night at 4°C ...
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bioRxiv - Genomics 2022Quote: ... The ConA bound nuclei were then incubated with primary antibody diluted 1:100 in Antibody Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Immunology 2022Quote: 100 μL overnight culture media of 100.000 cells/200 μL was analyzed on the CEDEX Bio Analyzer (Roche), using the Glutamine V2 Bio or Glucose Bio test kits.
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bioRxiv - Molecular Biology 2020Quote: ... and finally for 5 min in a 1:100 dilution of the substrate CSPD or CDP Star (Roche) in buffer 3.
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bioRxiv - Physiology 2020Quote: ... NaCl 150 mM and Triton X-100 150 mM) with the addition of a protease inhibitors cocktail (Roche), and mechanically disrupted through freeze-thaw cycles ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and incubated with G6B antibody diluted 1:100 in Ventana’s DISCOVERY antibody diluent (Roche Cat#760-108) for 60 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were resuspended in wash buffer [(10 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM EDTA pH 8.0, protease inhibitor (Roche)] and rotated for 10 min at 4°C ...
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bioRxiv - Genomics 2019Quote: ... 0.5 mM EGTA,1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 140 mM NaCl, 1x protease inhibitors-Roche). 150 ul of sonicated chromatin was then incubated 4h at 4°C on a rotating wheel with 3 µg of antibody conjugated to 10 µl of Dynabeads ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Molecular Biology 2019Quote: ... High Salt buffer (50 mM HEPES pH 7.5, 500 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... and 0.1% [v/v] Triton X-100) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, catalog no. 11873580001) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mM MgCl2) supplemented with 0.5% Triton X-100 and 1 pill Complete® protease inhibitor cocktail (Roche). The cell lysates were subjected to two 5 sec pulses of sonication at the lowest settings (Branson Sonifier 250 ...
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bioRxiv - Genetics 2020Quote: ... then resuspended in 600 µl of ChIP lysis buffer (50mM HEPES, pH 7.5, 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS twice and lysed with lysis Buffer II (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5% NP-40, 1 mM EDTA and EDTA-free protease inhibitors cocktail [Roche]). As a control of protein synthesis inhibition ...
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bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were resuspended in 10 ml ice cold buffer LB1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.50% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were resuspended in Lysis buffer (50 mM Tris pH 8.0, 300 mM NaCl, 2 mM DTT, 0.3 % TritonX-100, Roche Complete protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM MgCl2, 0.5% Triton X-100, 0.25% Nonidet P-40 and 10% glycerol, supplemented with protease inhibitors [Roche]). After a 10 minute incubation on ice ...
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bioRxiv - Microbiology 2021Quote: ... 1.1% Triton X-100, 1.2 mM EDTA, 20 mM Tris-HCl pH8.0, 167 mM NaCl, complete protease inhibitor [Roche]). Antibody (2 μg ...
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bioRxiv - Microbiology 2021Quote: ... 1.1% Triton X-100, 1.2 mM EDTA, 20 mM Tris-HCl PH8.0, 500 mM NaCl, complete protease inhibitor [Roche]), and LiCl (250 mM LiCl ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed twice with PBS before lysis in 0.1ml lysis buffer (1% Triton X-100, 0.1% SDS, PBS, Complete EDTA-free protease inhibitor [Roche]). Total protein concentration of lysates was quantitated using the Pierce BCA assay (cat no 23225 ...
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bioRxiv - Cell Biology 2019Quote: ... 50 mM Tris, pH 7.2, 125 mM NaCl, 2 mM DTT, 0.1% Triton X-100, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Cell Biology 2019Quote: Whole-cell lysates were obtained by incubating cells in lysis buffer (1% Triton X-100, 50mM HEPES pH 7.4, 140mM NaCl, 1.5mM MgCl2, 1mM EGTA, 10% glycerol; supplemented with Roche protease/phosphatase inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... 0.5 mM EGTA,1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 140 mM NaCl, 1x protease inhibitors-Roche) and once with Buffer-C (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... 1% Triton X-100, 0.1% deoxycholate Na, 0.1% SDS, 1 mM AEBSF [Euromedex] and cOmplete EDTA-free [Roche]) with glass beads (diameter ...
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bioRxiv - Genomics 2021Quote: Crosslinked MEFs per protocol above from 6-well dish were washed once in 2 mL PBS and collected in 1 mL cold NE1 buffer (20 mM HEPES pH 7.5, 10 mM KCl, 1 mM MgCl2, 1 mM DTT, 0.1% Triton X-100, Roche Protease Inhibitor Cocktail (Millipore (Burlington ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5% Triton X-100, 1% IGEPAL, 0.5% Sodium Deoxycholate, 0.1% SDS, 1 mM DTT, 1x Complete protease inhibitor [Roche]) for 30 minutes at 4°C under rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting pellets were resuspended in 1 ml of lysis buffer (Tris-Cl pH 8.0 20 mM, NaCl 150 mM, Triton X-100 0.5%, EDTA 0.5 mM, cOmplete mini [Roche] 3X), frozen in N2 and thawed on ice ...