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Citations for Roche :
301 - 350 of 1132 citations for Recombinant Human CLEC11A Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... fibronectin from human plasma (Roche), the final concentration used for the experiments was 50 μg/ml (containing 2/3 of bovine and 1/3 of human FN) ...
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bioRxiv - Developmental Biology 2019Quote: ... human holotransferrin 0.6% (Roche, 10652202001); monothioglycerol 0.0039 % (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Human fibronectin (Roche Diagnostics, 11051407001); Puromycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and human insulin (11376497, Roche) were digested with recombinant WT or protease-dead (cf-E111Q ...
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bioRxiv - Plant Biology 2020Quote: ... 4 µg recombinant NAA50 was incubated with 100 µM Acetyl-Coenzyme A (Roche) in a 2X acetylation buffer (50mM Tris HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... The extracted RNA was then treated with DNase I recombinant RNase free (Roche) and the reverse transcription was done using the Super-Script IV (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 μg of total RNA was incubated with recombinant DNase I (Roche, 04716728001) for 30 min at 37°C and the reaction was stopped by incubation at 75°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... Residual genomic DNA contamination was removed by treatment with recombinant DNAse I (Roche). cDNA was generated using Superscript II reverse transcriptase and Oligo d(T ...
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bioRxiv - Neuroscience 2022Quote: Recombinant lentiviruses were produced by transfecting HEK293T cells using FuGENE®6 (Roche) with plasmids encoding viral enzymes and envelope proteins essential for packing of viral particles (pRSV-EV ...
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bioRxiv - Microbiology 2023Quote: ... 1X antibiotic-antimycotic) with 100 U/mL recombinant interleukin (IL)-2 (Roche #11147528001) and activated or frozen in freezing media (90% FBS ...
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bioRxiv - Microbiology 2023Quote: DNase treatment was performed using recombinant RNase-free DNase I (Roche, Basel, Switzerland) according to the manufacturer’s protocols for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Macrophages were polarized with 20 ng/mL recombinant IL-4 (Peprotech or Roche) or 100 ng/mL LPS (E ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The in vitro transcription using the T7 RNA polymerase and the DIG RNA labelling Mix (both Roche) was performed following the supplier’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: Antisense riboprobes were transcribed using NEB T7 or T3 RNA polymerase and labeled with digoxygenin (DIG) (Roche). Whole-mount in situ hybridization (WISH ...
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bioRxiv - Neuroscience 2019Quote: ... which was labeled with fluorescein by using Fluorescein RNA Labeling Mix and T7 RNA polymerase (Roche Diagnostics). After blocking with PBS containing 2% normal goat serum for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: Riboprobes were made using T3 and T7 polymerases and a digoxigenin-labeled ribonucleotide mix (Roche, Cat.# 11277073910) using PCR templates ...
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bioRxiv - Developmental Biology 2021Quote: ... and used as template to generate DIG-labeled in situ probes with SP6/T7 polymerases (Roche, # 10999644001). Whole-mount in situ hybridization was done according to Lécuyer et al ...
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bioRxiv - Neuroscience 2022Quote: ... was synthesized by in vitro transcription using T7 RNA polymerase with a DIG RNA labeling kit (Roche). The template for transcription was made using PCR on zebrafish cDNA with the following primers ...
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bioRxiv - Developmental Biology 2023Quote: ... Riboprobes were generated by in vitro transcription using the SP6/T7 DIG RNA labelling kit (Roche, # 11175025910) after plasmid linearization as indicated in Table S4 ...
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bioRxiv - Developmental Biology 2023Quote: ... In vitro transcription was performed on the linearized template using T7 RNA polymerase (Roche DIG-labelling mix) to obtain a full-length Digoxigenin-labelled DTA riboprobe ...
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bioRxiv - Neuroscience 2023Quote: ... Linearized plasmids were then used as templates for in vitro transcription using either SP6 or T7 (Roche) using DIG labelled nucleotides (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were hybridized with a digoxigenin (DIG)-tagged antisense mRNA probes detected by an anti-DIG antibody (Roche) and developed in NBT/BCIP (Roche) ...
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bioRxiv - Bioengineering 2019Quote: ... DNA was amplified using the polymerase Kapa Hi Fi DNA polymerase (Kapa Biosystems, cat no. KK2602) and gel purified using Seakem GTG agarose (for nucleic acid recovery ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
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bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... lysed by sonication as described above and loaded onto the cOmplete His-tag purification column (Roche). The complex was eluted in 300 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
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bioRxiv - Evolutionary Biology 2023Quote: ... the supernatant was further incubated with 25 μl of cOmplete His-tag purification resins (Roche, Switzerland) at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... the cleared lysate was added to 1 mL cOmplete His-Tag Purification Resin (COHISR-RO, Roche) that had been pre-washed with buffer ...
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Human genomic DNA (Roche Cat#11691112001) was purchased from Sigma (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...
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bioRxiv - Cancer Biology 2020Quote: ... 180 μg/ml human transferrin (Roche). Flk-1+ cells were isolated by magnetic cell sorting (MACS ...
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bioRxiv - Developmental Biology 2021Quote: ... F0425)/500 µg protein with protein A-agarose (Roche). Fished-out FGFR3 protein was eluted and then denatured at 95°C for 10 minutes in NuPAGE LDS sample buffer (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA probes were generated via overnight incubation at 37°C using 20 U/µL T7 RNA polymerase (Roche) and digoxigenin (DIG)-labeled dNTPs (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... DIG-labeled antisense or sense probes were synthesized using T7 or T3 RNA polymerase (Roche, 10588423 and 11031171001) and Dig RNA Labeling Mix (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... Digoxigenin (DIG)-labeled probes were synthesized with DIG RNA labeling kit version 12 (SP6/T7) (Roche, Mannheim, Germany). Biotin-labeled probes were synthesized by substitution of DIG RNA labeling mix with biotin RNA labeling mix (Roche ...
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bioRxiv - Physiology 2020Quote: ... Synthesis of digoxigenin-labeled antisense RNA probes were synthesized using a DIG RNA Labeling Kit (SP6/T7; Roche).
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bioRxiv - Neuroscience 2023Quote: ... and DIG-UTP labelled cRNA probes synthetized from linearized plasmids using either SP6 or T7 RNA polymerases (Roche) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Probes were transcribed with TranscriptAid T7 High Yield Transcription kit (Thermo, KO441) using DIG-Label UTP (Roche,11127073910).
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was extracted from recombinant clones with the High Pure Isolation kit (Roche) as directed by the manufacturer and sequenced in an ABI PRISM 3100 genetic analyzer (Applied Biosystem ...
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bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 µL nuclease free water ...
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bioRxiv - Microbiology 2020Quote: ... and ultimately digested with 1.25 µg of recombinant Trypsin (sequencing grade; Roche, Mannheim, Germany) overnight at 37 °C (Sechi and Chait ...
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bioRxiv - Microbiology 2019Quote: ... 5 μg of total RNA were treated with recombinant DNase I (Roche Diagnostics Ltd.) at 37°C for 30 min and then purified using a standard phenol–chloroform method ...
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bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 μL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 μL nuclease free water ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg of total RNA were treated with recombinant DNase I (Roche Diagnostics Ltd) at 37°C for 30 min and then purified using a standard phenol–chloroform method ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.25 mM DTT) supplemented with 0.05 U/µl RNase-free recombinant DNase I (Roche), 0.4 U/µl RNasin (Promega ...