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Citations for Roche :
251 - 300 of 1132 citations for Recombinant Human CLEC11A Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... supplemented with 20 U/ml of recombinant huIL-2 (Roche, 11147528001) and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend ...
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bioRxiv - Microbiology 2022Quote: Removal of DNA was conducted with Recombinant DNase I (Roche Diagnostics) per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: 2 μl 1x proteinase K (recombinant PCR Grade, 25 mg - Roche) dissolved in 2,5 ml TE (10 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of RNA was treated with Recombinant DNase I (Roche) and cDNA was synthesized using Superscript IV reverse transcriptase (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was incubated with 100 μL of recombinant Proteinase K (Roche) diluted to ∼2 mg/mL in 1 x tris-EDTA (TE ...
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bioRxiv - Cell Biology 2021Quote: ... human plasma fibronectin (Roche) was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Human genomic DNA (Roche) was amplified ...
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bioRxiv - Developmental Biology 2022Quote: ... In vitro antisense RNA synthesis was performed using T7 RNA Polymerase (Roche, Cat. No. 10881767001) and DIG RNA Labeling Mix (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... and npbwr2 by using the DIG RNA Labeling Mix and T7 RNA polymerase (Roche Diagnostics). The procedure for in situ hybridization has been described previously (Hiraki et al. ...
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bioRxiv - Developmental Biology 2020Quote: The Cas9 mRNA were synthesized by in vitro transcription using T7 RNA polymerase (Roche, Mannheim). The target sites of gRNA starting with “GG” or “GA” were designed manually ...
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bioRxiv - Immunology 2022Quote: ... Plasmids were linearised and probes synthesised according to DIG RNA Labeling Kit (SP6/T7) (Roche). Zebrafish larvae were fixed in 4% paraformaldehyde solution (PFA ...
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bioRxiv - Developmental Biology 2022Quote: Digoxigenin RNA probes were synthesized using the T3/T7 Transcription Kit (Roche; Supp. Table 1). Larvae were collected ...
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bioRxiv - Developmental Biology 2019Quote: ... In vitro antisense RNA synthesis was performed using T7 RNA Polymerase (Roche, Cat. No. 10881767001) and DIG RNA Labeling Mix (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... DIG-labelled RNA probes were generated with DIG RNA Labeling Kit T7/SP6 (Roche #11175025910), see Table S3 for the primers used to generate the DNA templates ...
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bioRxiv - Developmental Biology 2022Quote: ... The RNA probes were synthesized using T7 RNA polymerase with DIG RNA Labeling Mix (Roche). The larvae were fixed in 4% formaldehyde at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... NEB Biolabs) and probes were synthesised according to DIG RNA Labelling Kit (SP6/T7, Roche). Zebrafish larvae were anaesthetised in 0.168mg/ml Tricaine (MS-222 ...
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bioRxiv - Developmental Biology 2023Quote: ... DIG or fluorescein labeled RNA probes were synthesized by T7 and sp6 RNA polymerases (Roche) from template DNA plasmid digested by NotI or SpeI (New England Biolabs) ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was added to a slurry of cOmplete His-Tag Purification Resin (Roche) and incubated for 1 h at room temperature or 2 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was added to a slurry of cOmplete His-Tag Purification Resin (Roche) or Ni Sepharose High Performance (Merck ...
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bioRxiv - Microbiology 2021Quote: ... Fab was purified from cell culture supernatant by cOmplete His-Tag Purification Resin (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Genomics 2024Quote: ... the dialyzed sample was loaded again onto a cOmplete His-tag purification column (Roche), and the untagged Tn5 was collected in the flow-through ...
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bioRxiv - Biochemistry 2020Quote: ... Fractionated samples were analyzed by SDS-PAGE and electrophoresis for detection of HA-tagged Nedd4 (Roche anti-HA high affinity,1:2000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... transfected with recombinant EMBacY BACs using X-tremeGENE DNA Transfection Reagent (Roche), and incubated for 72 h at 28 °C ...
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bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and treated with DNAse I (Dnase I recombinant RNAse-free, Roche Diagnostics). Then cDNA were produced from 5 μL of total RNA using RT Superscript III (RT Superscript III First Strand cDNA Synthesis Kit ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic DNA was removed by digestion using DNase I Recombinant (Roche, 04716728001) and RiboLock RNase Inhibitor (Thermo Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant baculovirus was generated by initial lipofection with Xtreme gene reagent (Roche) of Sf21 insect cells (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... and basic fibroblast growth factor (bFGF, 10 ng/ml; recombinant bovine, Roche). The cells were then plated in a 96-well plate in complete neurosphere medium containing DMEM/F-12 EGF and bFGF ...
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bioRxiv - Cancer Biology 2022Quote: ... 1x DNase buffer (500ul) and 200U recombinant DNase I (20ul, Roche, 04716728001) at 37 C in a shaker set at 100 RPM ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA contamination was removed through treatment with recombinant DNaseI (Roche Diagnostics, #04716728001) for 15 minutes at RT and column purification using Qiagen RNeasy Mini kit (#74106) ...
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bioRxiv - Neuroscience 2023Quote: ... DNA contaminations were eliminated with DNase I recombinant (#04716728001; Roche Applied Science) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or FITC-labeled antisense probes were synthesized using an RNA Labeling Kit (SP6/T7; Roche). The staining was revealed with NBT/BCIP or INT/BCIP substrate (Roche) ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... Riboprobes for in situ hybridization were synthesized using the DIG RNA labelling kit (Sp6/T7, Roche) following the manufacturer’s instructions ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... This was carried out using the DIG RNA Labeling Kit (SP6/T7) (Roche; Cat. No. 1175025) according to the manufacturer’s instructions (13µL template ...
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bioRxiv - Neuroscience 2021Quote: ... Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7) (Roche) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Probes were synthesized by in-vitro transcription using DIG labeling mix and T7 polymerase (Roche, USA). Hybridization was conducted as previously described (72) ...
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bioRxiv - Physiology 2022Quote: ... Digoxigenin (DIG) labeled probe was synthesized using the DIG RNA labeling kit (SP6/T7) (Roche, #11175025910). Denatured DIG-labeled probe was hybridized at 55° C overnight ...
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bioRxiv - Developmental Biology 2019Quote: Riboprobes for in situ hybridizations were synthesized using the DIG RNA labelling kit (Sp6/T7, Roche) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and riboprobes were prepared using the DIG RNA labeling mix and SP6/T7 transcription kit (Roche). Sections were fixed in 4% PFA for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Digoxigenin (DIG) RNA probes were obtained by in vitro transcription with T7 RNA polymerase (Roche Diagnostics) using PCR-generated DNA fragments as templates ...
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bioRxiv - Cell Biology 2023Quote: ... Dioxygenin (DIG)-labelled RNA probes were synthesized with T3 and T7 polymerase with reagents from Roche. Embryos were dechorionated with forceps and fixed in 4% paraformaldehyde in 1X PBS overnight ...
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bioRxiv - Neuroscience 2023Quote: ... which was labeled with fluorescein using T7 RNA polymerase and Fluorescein RNA Labeling Mix (Roche Diagnostics), and a DIG-labeled AR (ara ...
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bioRxiv - Physiology 2024Quote: ... The purified cDNA fragments were transcribed in vitro using T7 or SP6 RNA polymerase (Roche Diagnostics) in the presence of digoxigenin (DIG)-UTP ...
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bioRxiv - Microbiology 2021Quote: ... anti-human CD4 clone SP35 and anti-human CD8 clone SP57 (Roche, Basel, Switzerland), and anti-human CD20 clone L26 Dako Omnis (Agilent ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by PCR amplification with KAPA 2X Hi-Fi Hotstart Readymix (Roche, Cat. No. KR0370).
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bioRxiv - Biochemistry 2019Quote: ... Clear lysate was affinity-purified by incubating it with cOmplete His-Tag purification resin (Roche) for 2 hours (4°C) ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with the Complete His-Tag Purification Resin (Roche, Basel, Switzerland) that had been prewashed with Renz buffer ...
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bioRxiv - Cell Biology 2024Quote: ... supernatants were harvested and purified using His-Tag purification resin (Roche, cOmpelteTM, Cat No.5893682001), followed by size-exclusion chromatography in 20 M Hepes or Tris pH 8 ...
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bioRxiv - Bioengineering 2021Quote: ... Westernblot analysis of epitope-tagged PanK variants was performed with peroxidase-conjugated anti-HA antibody (Roche Diagnostics) and luminol-containing detection system ...