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Citations for Roche :
301 - 350 of 356 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... sgRNA templates were prepared according to (Bassett et al. 2013) and purified templates were in vitro transcribed using T7 RNA polymerase (Roche). 900 ng/µl of purified Cas9 mRNA and 400 ng/µl of purified guide RNA were mixed along with blue food dye and injected into eggs within 2 hours of egg laying ...
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bioRxiv - Biochemistry 2020Quote: ... His – tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 1 mL or 5mL cOmplete His-tag columns (Roche). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Exon 1 and exon 3 digestion products were purified using the High pure PCR product purification kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Titers of purified virus preparations were determined by quantitative PCR using a Roche Lightcycler 480 (Roche Applied Sciences, Pleasanton, CA) with primers amplifying the AAV2 ITR regions (forward ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting libraries were purified according to the Nextera XT protocol (0.6x ratio) and quantified by qPCR using Kapa Library Quantification (Kapa Biosystems). Four plates were pooled together on a HiSeq 2500 Rapid flow cell reading 25 bases in read 1 ...
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bioRxiv - Neuroscience 2021Quote: ... The libraries were then purified following the Nextera XT protocol (at 0.6× ratio) and quantified by qPCR using Kapa Library Quantification (Kapa Biosystems). A total of 6–10 plates were run on a NextSeq 550 high-output flow cell ...
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bioRxiv - Immunology 2022Quote: ... and 11-week infected half brains and liver lobes of mice (n=4/group) was extracted and purified using a High Pure PCR Template Prep Kit (Roche). DNA concentration of each sample was determined via NanoDrop ...
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bioRxiv - Genomics 2022Quote: ... The resulting pooled replicate amplicons were purified and three pyrosequencing runs were conducted on a 454 Life Sciences GS FLX (Roche) machine in accordance to the Roche Amplicon Lib-L protocol ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting libraries were purified according to the Nextera XT protocol (0.6x ratio) and quantified by qPCR using Kapa Library Quantification (Kapa Biosystems). Three plates were pooled on a NextSeq 550 mid-output flowcell with 26 bases in read 1 ...
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bioRxiv - Neuroscience 2022Quote: ... Both the concentrated non-exosomal supernatant and the purified exosomes were resuspended in RIPA Lysis and Extraction Buffer supplemented with cOmplete Mini EDTA-Free Protease Inhibitor Cocktail (Roche) and vortexed for 1 minute ...
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Purified plasmids were used to prepare digoxigenin-labelled sense and anti-sense riboprobes using T7 and SP6 RNA polymerases (Roche). Probes were purified using ethanol precipitation and resuspended in 1:1 volume of DEPC-treated water:formamide.
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bioRxiv - Biochemistry 2023Quote: ... SUPREM variants were purified by affinity chromatography in an open column using cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland), eluting with 20 mM HEPES pH 7.0 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Purified DNA was used for subsequent qPCR reactions using SYBR Green I Master Mix on a LightCycler 480 System (Roche). Enrichment was calculated using the following formula ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified libraries were controlled by capillary electrophoresis (LabChip - Perkin) and quantitated by qPCR (KAPA Library Quantification Kits Illumina Platforms - Roche). Pooled libraries (12 pM ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted and purified with the MagNA Pure LC instrument and the MagNA Pure DNA isolation kit III (Roche Diagnostics), according to the recommendations of the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... The purified products served as a template to synthesize digoxigenin-labelled antisense mRNA probes using DIG RNA labelling mix (Roche #11277073910).
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bioRxiv - Neuroscience 2020Quote: ... The resulting PCR products were purified and used as templates for in vitro transcription by employing DIG RNA Labeling Kit (SP6/T7) (Roche #11175025910). The resulting RNAs generated from SP6-mediated in vitro transcription were used as positive probes (cRNA ...
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bioRxiv - Plant Biology 2021Quote: ... The purified PCR product was then used for an in vitro transcription reaction using the DIG RNA Labeling Mix (#11175025910, Roche, Germany) and T7 and Sp6 RNA polymerase to generate both sense and antisense probes ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 600 ng of purified gDNA was used to construct a sequencing library using KAPA HyperPlus library preparation kit (Roche Diagnostics). Final library QC for size distribution verification was done on Caliper Lab Chip GX (Perkin Elmer ...
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bioRxiv - Immunology 2020Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Cell Biology 2021Quote: ... the small fragments from the cDNA amplification are purified and the library is barcoded and amplified using PCR with the KAPA high fidelity PCR kit(KAPA Biosystems). After all libraries are ready their quality and size are measured using the bioanalyzer before being sequenced on a NEXTseq (Illumina).
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bioRxiv - Synthetic Biology 2020Quote: ... 10 ul of purified bacmid were resuspended in 130 ul Sf-900 II media with 10 ul X-treme XP transfection reagent (Roche # 06366236001) and incubated at room temperature for 15 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... for overnight at 37°C followed by DNase treatment and purified using Quick Spin Columns for radiolabelled RNA purification (Roche, 11274015001). For Ψ and I modified RNAs synthesis ...
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bioRxiv - Genomics 2021Quote: ... Sequencing adapters were added in a second PCR using three reactions with 10 μl of the purified junction PCR product with the KAPA HotStart HiFi Ready Mix (KAPA Biosystems) for 12 cycles ...
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bioRxiv - Genomics 2021Quote: ... Four PCR reactions were then carried out with 1 μl of the purified DNA using the KAPA HotStart HiFi Ready Mix (KAPA Biosystems) for 10 cycles inserting a 15 nt homology sequence for the subsequent cloning step (IF_fwd/IF_rev) ...
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bioRxiv - Immunology 2021Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Molecular Biology 2023Quote: ... indexes and sequencing sequences) was performed on 5 µl of purified first step PCR products using Expand™ Long Template PCR System (Roche) with the following thermocycling program ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with λPPase or that purified from Calyculin A-treated cells was subjected to proteolytic digestion with 0.01 ng/µl trypsin (Roche, sequencing grade) for 0 ...
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bioRxiv - Cell Biology 2024Quote: ... and a sgRNA preceded by a rrk1 leader and followed by a Hammerhead Ribozyme as described in [17] was digested and prepared for gap repair as follows: the vector pJB166 was purified from DH5α bacterial cells using the Genopure plasmid midi kit (Roche, 03143414001) and diluted to 1 μg/μL ...
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bioRxiv - Microbiology 2019Quote: The pooled library was prepared with 10 μl of barcoded PCR bead purified product from each individual sample and measured with KAPA qPCR library quantification kit (KAPA Biosystems, USA), before being run on an Illumina Mi-Seq Sequencer using a 600-cycle pair end reagent kit (Mi-Seq Reagent Kits v2 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A pooled library was prepared from 10 μl of bead purified product from each population and checked with KAPA qPCR library quantification kit (KAPA Biosystems, USA); before being run on an Illumina MiSeq Sequencer using a 500-cycle pair end reagent kit (MiSeq Reagent Kits v2 ...
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bioRxiv - Neuroscience 2019Quote: ... and Qiazol Lysis (Reagent cat.no.79306, Hilden, Germany) purified on MagnaPure LC (HP Kit no.03542394001, F. Hoffmann - La Roche AG, Rotkreuz, Switzerland) and amplified via real-time PCR (4ng RNA/reaction ...
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bioRxiv - Biochemistry 2022Quote: ... 46 and 78 and C1213-3144 fragment were purified in the presence of complete protease and phosphatase inhibitor cocktails (Roche Applied Science) to retain phosphorylation as described previously for recombinant full-length human huntingtin proteins (Vijayvargia et al. ...
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bioRxiv - Microbiology 2021Quote: ... The purified indexed PCR library products were quantified using the KAPA Library Quantification Kit from Roche (cat. no. 07960255001, kit code KK4844), normalized to a concentration of 7 nM ...
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bioRxiv - Immunology 2020Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Immunology 2021Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Genomics 2022Quote: ... The resulting libraries were purified following the Nextera XT protocol (0.6× ratio) and quantified by qPCR using Kapa Library Quantification kit (Kapa Biosystems, Cat. KK4824). Four plates were pooled together on a NextSeq 550 high-output flow cell or NextSeq 2000 P2-100 flow cell with 26 bp in read 1 and 50 bp in read 2 ...
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bioRxiv - Physiology 2022Quote: ... The PCR products of target genes were purified and used as templates for probe preparation using DIG or fluorescein RNA labeling kits (Roche, Basel, Switzerland). The pituitary sections were hybridized with the antisense RNA probes for fshb and lhb as we previously reported [52] ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng of purified DNA for each IP and input sample were used for library preparation with the KAPA Hyper Prep Kit (Kapa Biosystems KR0961). TAZ and Combo treatments required two IPs per biological replicate to attain enough material for library preparation ...
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bioRxiv - Genomics 2023Quote: ... The cDNA was amplified by combining 25 μL of the silane purified product with PCR master mix (25 μL 2X Kapa HotStart Mix (Kapa Biosystems #KK2602), 2 μL of 5 μM SMART PCR primer ...
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bioRxiv - Genomics 2023Quote: ... Illumina-compatible sequencing libraries were prepared from 20 ng of purified DNA in 50 μL of TE buffer using the KAPA Hyperprep Kit with Library Amplification Primer Mix (Roche, CAT #07962363001) and 6 cycles of PCR amplification ...
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bioRxiv - Physiology 2023Quote: ... 500ng of total purified RNA was used for library preparation with the KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina Platforms (KAPA Biosystems KK8561) according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2024Quote: ... were purified over a 1-mL column of anti-protein C affinity matrix according to the manufacturer’s protocol (Roche, cat. no. 11815024001). 1 mM CaCl2 was added to the supernatant ...
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bioRxiv - Plant Biology 2020Quote: ... and identified by morphological features.41 Polymerase chain reaction (PCR) products were purified with the High Pure PCR Product Purification Kit (Roche Diagnostics, Mannheim, Germany), and sequenced in both directions by Macrogen Inc ...
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bioRxiv - Microbiology 2021Quote: 16S rRNA gene concentrations in purified genomic DNA extracts were quantified using SYBR Green I Master on a LightCycler 480 II (Roche Molecular Systems, Inc.). The primer pairs for Bacteria and Archaea were Bac908F_mod (5’ s-AAC TCA AAK GAA TTG ACG GG-3′ ...
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bioRxiv - Microbiology 2021Quote: ... The purified DNA was used for Illumina sequencing library construction using a KAPA Hyper Prep Kit (for Illumina) (KAPA Biosystems, Wilmington, MA, USA). Sequencing was performed on an Illumina HiSeq 2500 platform (San Diego ...
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bioRxiv - Genetics 2022Quote: ... Sense and antisense digoxigenin (DIG)-labeled probes were synthesized from the purified PCR product using DIG RNA Labeling Kit (Roche, cat. no.11175025910). All primer sequences were listed in Supplementary Information (Supplementary Table 3).
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bioRxiv - Plant Biology 2022Quote: ... The PCR products were purified and used to in vitro synthesize digoxigenin-labeled sense and antisense probes using T7 RNA polymerases (Roche Diagnostics, Mannheim, Germany). ZmSOS1 sense probe was used as the negative control.
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bioRxiv - Microbiology 2023Quote: DNA was purified from virus-infected Vero cells stock by a commercially available kit (High Pure Extraction Kit; Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions.