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Citations for Roche :
201 - 250 of 356 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Purified individually tagged libraries were quantified by qPCR using Kapa Lib Quant Kit (Roche Diagnostics). In conjunction with the qPCR Ct values we used a library size of 265 bp to calculate library molarity ...
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bioRxiv - Immunology 2020Quote: ... placed in DMEM containing 0.2 mg/mL Liberase Cl purified enzyme blend (Roche Diagnostics Corp.), and incubated for 1.5 hr at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Purified libraries were quantified using the KAPA library quantification kit (Kapa Biosystems, Wilmington, MA, USA) as per manufacturer’s instructions performed on an Applied Biosystems 7500 Fast real-time PCR machine (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Libraries were purified using the High Pure PCR Production Purification Kit (11732676001, Roche / Sigma-Aldrich). Libraries were validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR) ...
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bioRxiv - Plant Biology 2023Quote: ... Libraries were prepared with the purified PCR product by the Kapa DNA Hyper Kit (Roche) together with TruSeq DNA UD indexes for Illumina (Illumina ...
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bioRxiv - Physiology 2022Quote: ... The cDNA was ultimately purified using AMpure XP beads and quantified by qPCR assay86 (Roche Light Cycler 480 Instrument II ...
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bioRxiv - Bioengineering 2022Quote: ... S195A-GD-FXa protein was further purified on an anti-Protein C Affinity matrix (Roche). Maldi mass spectrometry showed that TFPI was glycosylated ...
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bioRxiv - Immunology 2023Quote: ... Amplified DNA libraries were purified by adding 1.3x volume of KAPA pure SPRI beads (Roche) to each sample and incubated for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... supernatants were harvested and purified using His-Tag purification resin (Roche, cOmpelteTM, Cat No.5893682001), followed by size-exclusion chromatography in 20 M Hepes or Tris pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... the annealed probes were purified with Quick Spin Columns for radio-labeled DNA Purification (Roche). ZNF410 full-length or ZNF410-ZF were overexpressed and harvested from COS-7 cells ...
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bioRxiv - Developmental Biology 2021Quote: ... The final enriched product (library, after PCR) was purified using KAPA purification beads (Roche 07983298001 - KK8002) and a dual-SPRI size selection was performed (with KAPA beads ...
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bioRxiv - Genomics 2020Quote: ... concentrators and purified in large volume columns (High Pure Viral Nucleic Acid Large Volume Kit, Roche) using 10X (2.5 ml ...
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bioRxiv - Microbiology 2021Quote: ... spanning the SARS-CoV-2 genome) were purified using Kapa HyperPure beads (Roche Molecular Systems Inc) and quantified using a Qubit fluorometer and dsDNA HS Assay Kit (Thermo Fisher Inc ...
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bioRxiv - Microbiology 2021Quote: ... Gel-purified indexed libraries were quantified using the KAPA library quantification kit (Kapa Biosystems, Wilmington, MA) using an Applied Biosystems 7500 real-time PCR machine ...
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bioRxiv - Bioengineering 2021Quote: ... pastoris culture medium containing the recombinant protein was purified by affinity chromatography Ni-NTA agarose (Roche).
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bioRxiv - Molecular Biology 2022Quote: ... cell culture supernatants were harvested and proteins were purified from the supernatants using tandem Ni2+ (Roche) and Strep-Tactin (IBA ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... cDC were purified from spleens of Flt3L-expanded mice following spleen digestion with DNase I (Roche) and collagenase type III and Nycodenz® density gradient centrifugation (Axis shield ...
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bioRxiv - Cell Biology 2021Quote: ... The barcoded libraries were purified and quantified using the Library Quantification Kit - Illumina/Universal (KAPA Biosystems) on a TaqMan 7500 RealTime PCR System ...
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bioRxiv - Biophysics 2020Quote: ... The expressed protein is first purified by affinity chromatography using Ni-NTA coated agarose beads (Roche). His6-tagged proteins are eluted using 250 mM imidazole ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified libraries were normalized by quantitative PCR (qPCR) using the KAPA Library Quantification Kit (KAPA Biosystems) and diluted to a final concentration of 10 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified pool was quantified by real-time PCR with the Kapa Biosystems Quantification Kit (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The purified PCR products were used to construct libraries by the Kapa DNA Hyper Kit (Roche) together with TruSeq DNA UD indexes for Illumina (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... The purified library was quantified using the KAPA library quantification kit (KAPA Biosystems, Wilmington, MA, USA) and sequenced on an Illumina Miseq platform (Illumina Inc ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids were purified from whole blood or serum using the MagNA Pure 96 system (Roche) with the DNA/Viral NA 2.0 kit and the Viral NA Plasma external lysis S.V ...
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bioRxiv - Plant Biology 2023Quote: ... The purified PCR products were prepared for the libraries using the Kapa DNA Hyper Kit (Roche) utilizing indexes from TruSeq DNA UD indexes for Illumina (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... were pooled and the amplicons of ∼450 bp were purified from 1.5% agarose (MP Roche, Germany) gel using the Wizzard SV Gel and PCR clean system (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting cDNA was purified using the High Pure PCR Product Purification Kit (Roche Applied Science) [44].
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bioRxiv - Physiology 2024Quote: ... The purified cDNA fragments were transcribed in vitro using T7 or SP6 RNA polymerase (Roche Diagnostics) in the presence of digoxigenin (DIG)-UTP ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was purified from one million cells with the High Pure RNA Isolation kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified library was purified and then quantified using KAPA library quantification kit (#07960255001, Roche, Basel, Switzerland) and Bioanalyzer (Agilent technologies) ...
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bioRxiv - Biochemistry 2022Quote: Reverse transcription and PCR were performed on 2µg of purified RNA using the Transcriptor Reverse Transcriptase (Roche). RNA was first subjected to DNAse (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... and msp300-Cy5 RNA was purified using a Sephadex G50 spin column (Roche miniQuick Spin RNA column), followed by EtOH precipitation ...
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bioRxiv - Microbiology 2021Quote: ... The reactions were incubated and then bead purified using KAPA Pure Beads (KAPA Biosystems, Wilmington, MA, USA). Next ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed and proteins were purified by Complete™ Lysis-M EDTA-free kit (Roche, 04719964001). Protein samples were run on Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Purified DNA was quantified by real-time PCR with KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems) and gene-specific primers using the CFX Touch Real-Time PCR Detection System (Bio-Rad) ...
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SARS-CoV-2 Point Mutation and Deletion Spectra, and Their Association with Different Disease OutcomebioRxiv - Microbiology 2022Quote: ... Purified DNA pools were further processed using the DNA library preparation kit Kapa Hyper Prep kit (Roche), during which each pool was indexed using SeqCap Adapter Kit A/B (Nimblegen ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Purified DNA was quantified by real time PCR with KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems) and gene specific primers using a CFX Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Physiology 2019Quote: ... Libraries were constructed from purified DNA using the KAPA DNA Library Preparation Kit for Illumina (Kapa Biosystems). Input libraries were prepared from each experimental replicate using 10 ng of DNA purified immediately following shearing ...
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bioRxiv - Microbiology 2019Quote: ... Purified parasites treated at 12°C for 1 h with freshly prepared 1 mg/mL pronase (Roche)/0.01% saponin/PBS before washing ...
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bioRxiv - Genomics 2021Quote: ... The adapter-ligated gDNA was purified and amplified in 20 reactions using KAPA HiFi master mix (Roche) and Oligos 5 and 6 which add the homology flanks for the NEB HiFi DNA assembly ...
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bioRxiv - Neuroscience 2022Quote: ... the probe was diluted in nuclease free water and purified in a mini Quick-Spin column (Roche).
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bioRxiv - Microbiology 2021Quote: ... 40% isopropanol) and DNA was purified using a High Pure Viral Nucleic Acid kit (Roche Life Science) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Purified DNA was then pre-amplified with indexed primers and HiFi HotStart Polymerase Ready Mix (KAPA Biosystems). After estimation of additional PCR cycles required110 ...
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bioRxiv - Microbiology 2023Quote: ... Purified total RNA was processed into RNA sequencing libraries with the KAPA mRNA Hyperprep Kit (Roche, USA). Quality control was tested before and after library preparation using an Agilent TapeStation 4200 and sequencing was performed by the Cleveland Clinic Genomics Core using the Illumina sequencing platform NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2020Quote: Purified total RNA from pHCEnC was prepared for RNA sequencing libraries using the KAPA mRNA HyperPrep Kit (Roche Sequencing Solutions ...
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bioRxiv - Developmental Biology 2021Quote: ... and ultra-purified water were mixed and pipetted into the plate and a Roche Light Cycler (Roche, city) was used to run a program consisting of 45 cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... the probe was purified by chromatography using a Sephadex G-50 column (mini Quick Spin DNA Columns, Roche).
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bioRxiv - Cancer Biology 2019Quote: ... The purified ChiP DNA was used to generate sequencing libraries using Hapa Hyper prep kit from Kapa Biosystems. Libraries were sequenced in single-end using Illumina Hiseq 2500 or Illumina Hi-seq 4000 at 50 cycles.
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bioRxiv - Plant Biology 2022Quote: ... The radioactive probe was purified chromatographically with a Sephadex G-50 column (mini Quick Spin DNA Columns, Roche). After hybridization ...