Labshake search
Citations for Roche :
301 - 350 of 3683 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... One-step qRT-PCR was performed using the Transcriptor One-Step RT-PCR Kit (Roche) by using 5μL of samples per each 20 μL reaction volume ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using FastStart Universal SYBR Green Master (Roche) and the StepOnePlus thermocycler (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified using the High Pure PCR Product Purification Kit (Roche, Germany) and subjected to electrophoresis in 1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... The PCR amplicon was purified using the High Pure PCR Product Purification Kit (Roche, Germany) to remove the contaminants and the amplicon was sequenced with forward and reverse primers using Big Dye Terminator v3.1 Cycle Sequencing kit on Applied Biosystems 3730 x l Genetic Analyzer ...
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bioRxiv - Microbiology 2021Quote: ... The PCR was undertaken using a Kapa 2G Robust PCR kit (Kapa Biosystems, Woburn, MA) in 25 µL reactions containing ...
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bioRxiv - Genetics 2022Quote: ... PCR was done using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems, Wilmington, Massachusetts, US). A microcapillary nucleic acid fragment analysis for quality control of the cDNA library was conducted using a Bioanalyzer ...
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bioRxiv - Pathology 2020Quote: ... Quantitative PCR measurement was performed using the Cobas z 480 PCR system (Roche, Bâle, Switzerland). Data were analyzed with the LightCycler 480 SW 1.5 software (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... The RT product was amplified by real-time PCR (Roche LightCycler® 480 PCR instrument) using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™) ...
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bioRxiv - Molecular Biology 2020Quote: ... The RT product was amplified by real-time PCR (Roche LightCycler® 480 PCR instrument) using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR amplification was performed using KAPA HiFi HotStart Ready Mix PCR kit (Roche KK2602/07958935001); 2 μL forward and reverse primer pairs (10 μM each) ...
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bioRxiv - Microbiology 2021Quote: ... PCRs were routinely performed with the High-Fidelity Template PCR system (Roche Diagnostics, Barcelona, Spain) using an MJ Mini personal thermal cycler (Bio Rad ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on a LightCycler® 480 (Roche Diagnostics). Assays were made in triplicates and results normalized according to the expression levels of TBP ...
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bioRxiv - Genetics 2020Quote: ... and 0.5 U KAPA2G Fast PCR enzyme (KAPA2G Fast PCR kit; KAPA Biosystems, Wilmington, USA). Amplification was performed on the Takara PCR Thermal Cycler (Takara ...
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bioRxiv - Microbiology 2022Quote: ... quantitative RT-PCR (qRT-PCR) was carried out using a Light-Cycler 480 (Roche, France). As internal controls for mRNA quantification ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed with the LightCycler 480 Real-Time SYBR Green PCR System (Roche), used primers are listed in Table S2 ...
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bioRxiv - Physiology 2022Quote: ... Quantitative PCR of cDNAs was performed using the Quantitect SYBR green PCR Master mix (Roche) with 10 ng of cDNA (by NanoDrop ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was performed on a Light-Cycler 480 real-time PCR machine (Roche, Switzerland) by using PrimeScript™ RT reagent (Perfect Real Time ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative real-time PCR was performed on a LightCycler 480 II PCR system (Roche, Switzerland) with SYBR Green qPCR Master Mix (TaKaRa Bio) ...
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bioRxiv - Molecular Biology 2024Quote: ... maxi- and minicircles were PCR amplified and labelled using PCR DIG Probe Synthesis Kit (Roche). The detection was done using DIG Luminiscent Detection Kit (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time RT-PCR was carried out using Real-Time PCR Master Mix (KAPA Biosystems) and following probes and primers on a 7300 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... Whole transcriptome amplification (WTA) was achieved via PCR using KAPA Hifi PCR Mastermix (Kapa Biosystems). The WTA product was purified using Agencourt Ampure beads (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2022Quote: ... We conducted a PCR reaction using the Expand Long Range PCR Kit (Roche, Basel, Switzerland) and the TuGluCl2in primers ...
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bioRxiv - Microbiology 2023Quote: ... vector fragments and inserts were amplified by PCR using the KAPA HIFI PCR Kit (Roche) and assembled using a Gibson Mix for 30 min at 50°C ...
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bioRxiv - Plant Biology 2024Quote: ... was used for Quantitative RT-PCR (qRT-PCR) on a Roche LightCycler 480 system (Roche). Tubulin was used as an internal control gene ...
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bioRxiv - Bioengineering 2024Quote: ... RT-PCR was performed on the LigthCycler 480 Real-Time PCR System (Roche, Basel, Switzerland), and the cycling parameters were 1 cycle at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... maxi- and minicircles were PCR amplified and labelled using PCR DIG Probe Synthesis Kit (Roche). The detection was done using DIG Luminiscent Detection Kit (Roche ...
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bioRxiv - Microbiology 2024Quote: ... PCR was done with the Kapa HiFi PCR kit (KAPA Biosystems, Cape Town, South Africa) using 5 μl of extracted DNA as template and the following cycling conditions ...
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bioRxiv - Neuroscience 2024Quote: ... SYBR Green PCR master mix was used on the LC480 Real-Time PCR System (Roche) (n = 5 samples/group) ...
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bioRxiv - Immunology 2024Quote: ... PCR amplification of the gp33 minigene was performed using a KAPA HiFi PCR Kit (Roche). Primers targeting murine GAPDH were used to generate loading controls for each sample (5’ – 3’) ...
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bioRxiv - Microbiology 2020Quote: ... Kapa’s HyperPlus library preparation kit (Roche Sequencing Solutions) was used to prepare sequencing libraries from the double-stranded cDNAs ...
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bioRxiv - Microbiology 2020Quote: ... membranes were blocked with blocking solution (#11585762001, Roche) for 45 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated in BM purple solution (Roche) at RT protected from light until the purple color was developed.
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bioRxiv - Cell Biology 2020Quote: ... and with Casein solution (ref: 760-219, Roche) for 60 and 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... animals were placed in blocking solution (0.5% Roche Western Blocking Reagent and 5% inactivated horse serum for POD and AP antibodies and 0.2% BSA for DNP antibodies diluted in PBSTx) ...
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bioRxiv - Immunology 2021Quote: ... washed again and incubated with ABTS solution (Roche). The reaction was stopped with 1 μl/ml H2O2 ...
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bioRxiv - Immunology 2022Quote: ... including 5% Western Blocking Reagent Solution (#11921673001, Roche) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μl of MTT solution (Roche, USA, 11465007001) was added to each well and the whole experiment was performed according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... in 5% blocking solution (Roche, cat. no.11096176001) at room temperature overnight ...
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bioRxiv - Plant Biology 2023Quote: ... A blocking solution (11585762001, Roche Diagnostics, Indianapolis, USA) was injected into the 1 mm hole ...
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bioRxiv - Neuroscience 2024Quote: ... Chemiluminescent detection was performed using ECL solution (Roche). Following densitometry-based quantification and analysis using ImageJ software (National Institute of Health ...
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bioRxiv - Cancer Biology 2024Quote: ... red blood cell lysis solution (Roche, Cat#11814389001) was added to resuspend the cells ...
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bioRxiv - Developmental Biology 2024Quote: ... and color reagent NBT/BCIP staining solution (Roche). Chicken Lmx-1 ...
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bioRxiv - Cell Biology 2024Quote: ... tissues were then dissociated in Liberase solution (Roche, Liberase TM Research Grade ...
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bioRxiv - Immunology 2021Quote: Single cells were sorted directly into PCR tubes containing 25 μl PCR lysis buffer (1x Buffer 1 from the Expand Long Template PCR System [Roche], 0.5 mg/ml Proteinase K) and lysed for 1h at 55°C ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 1 mM DTT and incubated at RT in 0.05 mg/mL sequencing grade trypsin (Roche Applied Science). Digestions were stopped with 2X Laemmli sample buffer and placed on ice until analysis ...
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bioRxiv - Neuroscience 2021Quote: ... Briefly, ganglia from adult Aplysia were treated with Dispase® II (10 units/ml) (neutral protease, grade II) (Roche, Indianapolis, IL) at 35°C for ∼3 h and then desheathed ...
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bioRxiv - Immunology 2021Quote: ... We resuspended the glomerular fraction in 1mL HBSS buffer with Ca2+ and Mg2+ (Hyclone, Logan, UT) containing 0.1-0.2 mg/mL Liberase™Research Grade (Roche, Mannheim, Germany), and incubated glomeruli in 24-well tissue culture plates at 37°C for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with λPPase or that purified from Calyculin A-treated cells was subjected to proteolytic digestion with 0.01 ng/µl trypsin (Roche, sequencing grade) for 0 ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...