Labshake search
Citations for Roche :
251 - 300 of 3683 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... and iii) indexing PCR (KAPA HiFi HotStart ReadyMix PCR Kit, Roche, cat. no. KK2601). Sequencing was performed on a single Illumina NextSeq run (NextSeq 500/550 High Output Kit v2.5 (75 Cycles) ...
-
bioRxiv - Microbiology 2022Quote: ... All PCR products were purified with the High Pure PCR Product Purification Kit (Roche) before being used for TAR cloning.
-
bioRxiv - Developmental Biology 2022Quote: ... PCR reactions were performed on a LightCycler 480 II Real Time PCR Instrument (Roche) and analyzed using LightCycler 480 Software ...
-
bioRxiv - Genomics 2022Quote: ... PCR was performed by adding 10μl 2×HiFi PCR mix (Kapa Biosystems, Cat. 7958927001) and 0.5μl 60mM SINGV6 primer and running the following program ...
-
bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed with Roche LightCycler 480 Real Time PCR system (Roche, Switzerland) using LightCycler 480 SYBR Green Master (Roche ...
-
bioRxiv - Microbiology 2022Quote: ... All PCR products were purified by the High Pure PCR Product Purification Kit (Roche) before TAR cloning.
-
bioRxiv - Microbiology 2022Quote: ... The PCR product was purified with a PCR clean-up kit (Roche Applied Science) and end-labelled with T4 polynucleotide kinase (New England Biolabs) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantitative PCR (qRT-PCR) was performed using Light Cycler 480 instrument (Roche Life Sciences). Anti-Flag® M2 affinity agarose gel was from Sigma-Aldrich (A2220) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified with the High-Pure PCR Product Purification kit (Roche) and the library was sequenced by Illumina X ten platform.
-
bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified with the High-Pure PCR Product Purification kit (Roche) and then sequenced by the X ten platform.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The real time PCR program of the quantitative PCR (LightCycler480 II, Roche, Basel, Switzerland) was arranged as follows ...
-
bioRxiv - Microbiology 2024Quote: ... and 8 to 10 PCR cycles with the KAPA HiFi PCR kit (Kapa Biosystems) were used for amplification ...
-
bioRxiv - Pathology 2023Quote: Quantitative PCR (qPCR) was performed using the Quantitect SYBR green PCR Master mix (Roche) with 10 ng of DNA and 0.5 μM primers in a final volume of 10 μl ...
-
bioRxiv - Cell Biology 2023Quote: ... we used PCR with a proofreading Taq polymerase (Roche, Expand Long Template PCR System) to amplify the C-terminal region (∼0.5-1 kb ...
-
bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was conducted on Roche Lightcycler 96 Real time PCR system (Roche) with FastStart Essential DNA Green Master (Roche ...
-
bioRxiv - Genetics 2021Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... and quantitative PCR (Kapa Biosystems), according to the manufacturers’ instructions ...
-
bioRxiv - Genomics 2021Quote: ... and quantitative PCR (KAPA Biosystems).
-
bioRxiv - Genomics 2021Quote: ... and quantitative PCR (KAPA Biosystems).
-
bioRxiv - Genetics 2020Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2020Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturers’ instructions ...
-
bioRxiv - Genetics 2020Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturers’ instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturers’ instructions.
-
bioRxiv - Developmental Biology 2022Quote: ... purified (PCR cleanup kit, Roche) and transcribed (SP6 mMessage mMachine Kit Ambion) ...
-
bioRxiv - Genetics 2021Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... and quantitative PCR (Kapa Biosystems). Libraries were pooled and sequenced 75bp single end on the NextSeq (Illumina ...
-
bioRxiv - Systems Biology 2022Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturer’s instructions.
-
bioRxiv - Systems Biology 2022Quote: ... and quantitative PCR (KAPA Biosystems), according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and quantitative PCR (KAPA Biosystems). The sequencing library was clustered on one lane of a flowcell and loaded on the Illumina HiSeq instrument (4000 or equivalent ...
-
bioRxiv - Cell Biology 2024Quote: ... and quantitative PCR (KAPA Biosystems). Barcoded libraries were then pooled and sequenced on the HiSeq2000 (Illumina ...
-
bioRxiv - Cancer Biology 2023Quote: ... and q-PCR (Roche, KK4835), and sequenced by Illumina NovaSeq 6000.
-
bioRxiv - Molecular Biology 2023Quote: ... and quantitative PCR (KAPA Biosystems) method were used to quantify each library before multiplexing and the distribution of reads lengths were measured by the Agilent Tapestation 4200 (Agilent Technologies) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and quantitative PCR (KAPA biosystems). Library loading ...
-
bioRxiv - Developmental Biology 2024Quote: ... in PCR buffer 1 (Roche)) ...
-
bioRxiv - Cell Biology 2021Quote: ... The gel piece was vacuum dried and treated with sequencing grade trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche) at 37°C for 10-16 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... 15 µg total protein per sample was reduced (10 mM DTT, 30 min, 30 °C), carbamidomethylated (55 mM CAA, 30 min, room temperature) and digestion with trypsin (proteomics grade, Roche) overnight at 37°C at a 1:50 enzyme ...
-
bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
-
bioRxiv - Biophysics 2024Quote: ... Tumor spheroids were digested into single cell suspensions using 0.028 Wünsch units/ml of Liberase TM Research Grade (Roche, Mannheim, Germany) and 20 μg/ml of DNase I in L-15 with 10 % CS for 1 hour at 37 °C and 250 RPM on an orbital shaker ...
-
bioRxiv - Neuroscience 2023Quote: ... Each lane was excised into 9 sections that were destained and in-gel digested overnight using trypsin (sequencing grade; Roche). Peptides were extracted from gel bands twice with 50% acetonitrile:0.5% formic acid and dried in a SpeedVac (Thermo) ...
-
bioRxiv - Developmental Biology 2022Quote: ... samples were incubated with 27 µl labeling solution plus 3 µl enzyme solution (In Situ Cell Death Detection Kit, AP, Roche) at 37°C overnight ...
-
bioRxiv - Developmental Biology 2022Quote: ... The liver was dissected from the abdominal cavity and placed into a solution containing Hanks’ buffered saline solution (HBSS) supplemented with 1.07 Wünsch units/ml Liberase DH (Roche Applied Science) and 70 U/ml hyaluronidase (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2023Quote: Cell pellets were resuspended in a lysis solution composed by 1 X Phosphate Saline Solution (PBS) supplemented with 1 X Complete Protease Inhibitor Cocktail (Roche) and then sonicated with a BioRuptor UCD-200 (Diagenode ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR products were purified using the High Pure PCR product Purification Kit (Roche, Denmark). Subsequently the purified PCR products were sent for sequencing at LGC Genomics (Berlin ...
-
bioRxiv - Zoology 2021Quote: ... Fungal ITS1 fragments were amplified by PCR using the FastStart High Fidelity PCR System (Roche) for environment matrixes and the Hot Start High-Fidelity DNA Polymerase (NEB ...
-
bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on LightCycler® 480 (Roche, Basel, Schweiz) using AbsQuant 2nd Derivative Max for obtaining the Ct value ...
-
bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed with the LightCycler 480 Real-Time SYBR Green PCR System (Roche), used primers are listed in (Supplemental Dataset 5) ...
-
bioRxiv - Plant Biology 2020Quote: ... qRT-PCR experiments were performed in a Light-Cycler480 Real-Time SYBRgreen PCR system (Roche). 500 ng of RNA was reverse-transcribed with the qScript® cDNA Supermix kit (QuantaBio ...
-
bioRxiv - Microbiology 2020Quote: ... joining the individual fragments by overlap PCR using the Expand Long Template PCR System (Roche)104 and adding the PCR product to B ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Standard PCR- free libraries were prepared using KAPA Hyper PCR-free library reagents (KAPA Biosystems). Total genomic DNA was sheared into fragments of approximately 200-600 bp and purified using AMPure XP beads ...