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Citations for Roche :
3301 - 3350 of 8093 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... were incorporated by PCR (4 cycles) using KAPA HiFi Hotstart ReadyMix (Roche).
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bioRxiv - Genetics 2023Quote: Candidate regions were PCR-amplified using human genomic DNA (Roche, Basel, Switzerland) as template and cloned in pGL4.23 Firefly luciferase reporter vector (Promega ...
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bioRxiv - Immunology 2023Quote: ... The cDNA was amplified by PCR using KAPA HiFi HotStart ReadyMix (Roche), using M2-2 and SEQ7 (5’-ATATCGTCTCGTATTAGTAGAAACAAGGTAG-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... using GAPDH as an endogenous control on LightCycler 96 PCR system (Roche) using Faststart Essential DNA master mix (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative reverse transcriptase–PCR was performed using SYBR Green Master Mix (Roche) in a CFX384 machine (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR (qPCR) was performed on LightCycler® 480 Instrument II (Roche) using the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Immunology 2023Quote: ... PCR primers were designed by LightCycler® Probe Design Software from Roche. Real-time PCR was performed using custom TaqMan Gene Expression Assay reagent on QuantStudio 12K Flex Real-Time PCR System (Thermo Scientific).
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR analysis was performed with a LightCycler 480 engine (Roche) using Light-Cycler480 SYBR Green I Master Mix (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... All PCR reactions were carried out using the KAPA-HiFi enzyme (Roche). The reaction products were run on a gel (1% agarose in TAE) ...
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bioRxiv - Genomics 2023Quote: ... we used KAPA SYBR FAST One-Step qRT-PCR master mix (Roche), which is known to contain such additives ...
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bioRxiv - Biochemistry 2023Quote: ... by PCR (Table S2) using KAPA HiFi HotStart Ready Mix (Roche, 07958935001). Plasmid sequences were confirmed by Sanger sequencing.
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bioRxiv - Physiology 2023Quote: ... qRT-PCR was carried out with Lightcycler 96 (Roche Diagnostics, Basel, Switzerland), using KAPA SYBR Fast qPCR kit (NIPPON Genetics Co. ...
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bioRxiv - Neuroscience 2023Quote: ... fragments were generated by PCR with KAPA HiFi HotStart DNA polymerase (Roche) or by DNA synthesis (IDT) ...
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bioRxiv - Neuroscience 2023Quote: ... All PCR based cloning steps were performed using KAPA HiFi taq (Roche) and verified by sequencing ...
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bioRxiv - Pathology 2023Quote: ... Quantitative PCR analyses using Sybr (LightCycler 480 SYBR Green I Master, Roche) were performed using a LightCycler 480 real-time PCR system and dedicated software (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... For PCRs we used Taq polymerase (KAPA Biosystems, Cape Town, South Africa) and for qPCRs the FIREPol EVAGreen kit (Solis Biodyne ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR amplified using the KAPA HiFi HotStart ready mix (KAPA Biosystems) for 8 PCR cycles (45 s 98°C of initial denaturing and amplification at 15 s at 98°C ...
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bioRxiv - Cell Biology 2024Quote: ... Quantification of the libraries was performed using quantitative PCR (KAPA Biosystems KK4824). Finally ...
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bioRxiv - Microbiology 2024Quote: ... A second PCR was performed with the KAPA HiFi Hotstart ReadyMix (Roche) and 10 µM of the two primers 515F and 806R to amplify the V4 hypervariable region of the 16S rRNA ...
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bioRxiv - Bioengineering 2024Quote: ... Amplicons for sequencing were generated by PCR using KAPA HiFi HotStart (Roche) using primers with overhangs compatible with Nextera XT indexing (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... Magnetic beads were washed 2 times with lysis buffer and 4 times with washing buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mg/ml Pefabloc SC (Roche), and EDTA-free protease inhibitor cocktail (cOmplete Tablets ...
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bioRxiv - Molecular Biology 2020Quote: ... washed six times with phosphate buffered saline (PBS) and resuspended in 0.6ml of PBS supplemented with protease inhibitory cocktail (Roche) as per manufacturer’s specification ...
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bioRxiv - Genetics 2019Quote: ... DNA was eluted in 100μl of TE + 1%SDS and the beads were washed and eluted a second time with 100μl of TE + 1%SDS + 5 μl of 20mg/mL Proteinase K (Roche). Following overnight incubation at 65°C ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads were washed three times with wash buffer supplemented with Complete Mini EDTA-free Protease inhibitor cocktail (Roche, 04693159001) and PhosSTOP (Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed 3 times for 5 min each in PBST and blocked with 1X Blocking Reagent (Roche) in PBST ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was retrieved into a new 2 ml Eppendorf tube and diluted 10 times with RIPA buffer 0% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, protease inhibitor cocktail (Roche)) to obtain a concentration of 0.1% sodium dodecyl sulfate ...
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bioRxiv - Developmental Biology 2021Quote: ... Then the embryos were washed 10 times with PBST and the signal was developed in NBT/BCIP solution (Roche). For double in situ hybridization ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then washed eight times with PBST followed by the addition of 50 μL ABTS solution (Roche, #11684302001) into each well ...
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bioRxiv - Neuroscience 2020Quote: Whole cerebral organoids were washed three times in 1× PBS and dissociated in 2 mL of Accutase (StemPro) containing 0.2 U/μL Dnase I (Roche) for 45 min at 37°C ...
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bioRxiv - Immunology 2019Quote: Purified B cells were cultured either in medium alone or in the presence of 1.56-25ng ml− of recombinant BAFF for the indicated time and stained with Annexin V (Roche) and 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Molecular Biology 2022Quote: ... Blood was sampled from the tail vein at indicated time points using an Accu-Check II glucometer (Roche Diagnostics). For insulin measurements ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS 3 times and lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche), 0.1% Benzonase (Millipore-Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... heads were rinsed for 5 minutes in PBST five times and incubated in Blocking Reagent (Roche, 1:5000 dilution) for 60 minutes ...
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bioRxiv - Immunology 2024Quote: ... NK cells were cultured for the indicated amount of time with or without human IL-2 (TECINTM; teceleukin, ROCHE), human IL-15 (247-IL/CF ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently washed with PBS three times and nuclei were counterstained with DAPI (2.5 μg ml−1, Roche) before mounting with Prolong Gold (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Washed three times for 30 minutes each in TNTT at 4 ℃ to remove methanol.Blocked with 1% blocking reagents (Roche) in TNTT buffer for 2 hours at 4 ℃ to prevent no specific binding ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitates were washed three times with lysis buffer containing 1x cocktail of protease inhibitors from Roche (Complete, EDTA free) and were then transferred to new tubes ...
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bioRxiv - Bioengineering 2019Quote: ... RT-qPCR assay was performed in a Roche LightCycler® 480 Instrument II using LightCycler® 480 SYBR Green I Master (Roche Applied Science, Germany), RT Enzyme Mix (ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... and the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems) quantified the prepared libraries ...
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bioRxiv - Bioengineering 2021Quote: ... pseudosite attD plasmids were PCR amplified using the KAPA Hifi HotStart ReadyMix (Roche), amplifying the pEF-1α promoter followed by mCherry ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was performed using Thunderbird SYBR qPCR mix (Toyobo) and LightCycler96 (Roche). Data were analyzed with ΔΔCt method using siLuc and Gapdh as controls ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR product was purified from gel cut with ClaI (cat#10656291001, Roche) and BamHI-HF (cat# R3136s ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR analysis was performed with technical duplicates using SYBR Green PCR mix (Roche) for genes of interest and Gapdh (a normalization control for amplification efficiency ...
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bioRxiv - Genetics 2021Quote: ... qRT-PCR was performed with FastStart Essential DNA Green Master (Roche; Rotkreuz, Switzerland) with approx ...
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bioRxiv - Genomics 2020Quote: ... and performed PCR amplification with the KAPA HIFI Uracil+ Hotstart Ready Mix (Roche). Finally ...
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bioRxiv - Microbiology 2022Quote: ... Samples were prepared for PCR with Kapa HiFi HotStart ReadyMix (Roche, Cat # 07958935001) using the following primers:
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bioRxiv - Molecular Biology 2020Quote: Quantitative PCR was performed with the FastStart Universal SYBR Green Master Mix (Roche) using a 7900HT Fast Real-Time PCR System in a 384-Well Block Module (Applied Biosystems™) ...
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bioRxiv - Molecular Biology 2021Quote: ... The quantitative (q)PCR reactions were performed on a LightCycler 480 II (Roche), and analyzed by the ΔΔCT method with primers provided in Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR analyses were performed using the LightCycler 96 System (Roche Applied Science) with SYBR Premix Ex Taq (Takara) ...