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Citations for Roche :
3251 - 3300 of 8038 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads were washed three times with wash buffer supplemented with Complete Mini EDTA-free Protease inhibitor cocktail (Roche, 04693159001) and PhosSTOP (Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed 3 times for 5 min each in PBST and blocked with 1X Blocking Reagent (Roche) in PBST ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was retrieved into a new 2 ml Eppendorf tube and diluted 10 times with RIPA buffer 0% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, protease inhibitor cocktail (Roche)) to obtain a concentration of 0.1% sodium dodecyl sulfate ...
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bioRxiv - Developmental Biology 2021Quote: ... Then the embryos were washed 10 times with PBST and the signal was developed in NBT/BCIP solution (Roche). For double in situ hybridization ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then washed eight times with PBST followed by the addition of 50 μL ABTS solution (Roche, #11684302001) into each well ...
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bioRxiv - Neuroscience 2020Quote: Whole cerebral organoids were washed three times in 1× PBS and dissociated in 2 mL of Accutase (StemPro) containing 0.2 U/μL Dnase I (Roche) for 45 min at 37°C ...
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bioRxiv - Immunology 2019Quote: Purified B cells were cultured either in medium alone or in the presence of 1.56-25ng ml− of recombinant BAFF for the indicated time and stained with Annexin V (Roche) and 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Molecular Biology 2022Quote: ... Blood was sampled from the tail vein at indicated time points using an Accu-Check II glucometer (Roche Diagnostics). For insulin measurements ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then washed with PBS 3 times and lysed with RIPA buffer supplemented with protease inhibitor cocktail (Roche), 0.1% Benzonase (Millipore-Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... heads were rinsed for 5 minutes in PBST five times and incubated in Blocking Reagent (Roche, 1:5000 dilution) for 60 minutes ...
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bioRxiv - Immunology 2024Quote: ... NK cells were cultured for the indicated amount of time with or without human IL-2 (TECINTM; teceleukin, ROCHE), human IL-15 (247-IL/CF ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently washed with PBS three times and nuclei were counterstained with DAPI (2.5 μg ml−1, Roche) before mounting with Prolong Gold (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Washed three times for 30 minutes each in TNTT at 4 ℃ to remove methanol.Blocked with 1% blocking reagents (Roche) in TNTT buffer for 2 hours at 4 ℃ to prevent no specific binding ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitates were washed three times with lysis buffer containing 1x cocktail of protease inhibitors from Roche (Complete, EDTA free) and were then transferred to new tubes ...
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bioRxiv - Bioengineering 2019Quote: ... RT-qPCR assay was performed in a Roche LightCycler® 480 Instrument II using LightCycler® 480 SYBR Green I Master (Roche Applied Science, Germany), RT Enzyme Mix (ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... and the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems) quantified the prepared libraries ...
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bioRxiv - Bioengineering 2021Quote: ... pseudosite attD plasmids were PCR amplified using the KAPA Hifi HotStart ReadyMix (Roche), amplifying the pEF-1α promoter followed by mCherry ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was performed using Thunderbird SYBR qPCR mix (Toyobo) and LightCycler96 (Roche). Data were analyzed with ΔΔCt method using siLuc and Gapdh as controls ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR product was purified from gel cut with ClaI (cat#10656291001, Roche) and BamHI-HF (cat# R3136s ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR analysis was performed with technical duplicates using SYBR Green PCR mix (Roche) for genes of interest and Gapdh (a normalization control for amplification efficiency ...
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bioRxiv - Genetics 2021Quote: ... qRT-PCR was performed with FastStart Essential DNA Green Master (Roche; Rotkreuz, Switzerland) with approx ...
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bioRxiv - Genomics 2020Quote: ... and performed PCR amplification with the KAPA HIFI Uracil+ Hotstart Ready Mix (Roche). Finally ...
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bioRxiv - Microbiology 2022Quote: ... Samples were prepared for PCR with Kapa HiFi HotStart ReadyMix (Roche, Cat # 07958935001) using the following primers:
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bioRxiv - Molecular Biology 2020Quote: Quantitative PCR was performed with the FastStart Universal SYBR Green Master Mix (Roche) using a 7900HT Fast Real-Time PCR System in a 384-Well Block Module (Applied Biosystems™) ...
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bioRxiv - Molecular Biology 2021Quote: ... The quantitative (q)PCR reactions were performed on a LightCycler 480 II (Roche), and analyzed by the ΔΔCT method with primers provided in Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR analyses were performed using the LightCycler 96 System (Roche Applied Science) with SYBR Premix Ex Taq (Takara) ...
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bioRxiv - Plant Biology 2020Quote: ... Transformation was confirmed by colony PCR using KAPA2G Robust HotStart Polymerase (Kapa Biosystems).
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bioRxiv - Cell Biology 2019Quote: ... Each q-PCR reaction contained 2.5 μl of Sybr Green mastermix 2X (Roche, LightCycler 480 SYBR Green I Master ...
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bioRxiv - Plant Biology 2019Quote: ... and PCR was performed using SybrGreen (Roche Cat. No. 04 707 516 001) on a LightCycler 480 (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative PCR was preformed using 2X SYBR Fast Master Mix Universal (Kapa Biosystems) and primers for the indicated genes (see Table S3 for primer sequences ...
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bioRxiv - Genomics 2020Quote: ... The PCR reactions were carried out by KAPA Taq DNA Polymerase (KAPA biosystems). PCR amplification cycles were optimized in order to correspond to the logarithmic phase of each reaction ...
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bioRxiv - Biochemistry 2020Quote: ... All components were PCR amplified by using KAPA HiFi HotStart ReadyMix (Roche, Switzerland), the template vector was removed by DpnI treatment (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... All PCR reactions were done by using KAPA HiFi HotStart ReadyMix (Roche, Switzerland). Purified megaprimers (200 ng of DNA ...
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bioRxiv - Neuroscience 2021Quote: ... Genomic DNA was amplified using a Expand High Fidelity PCR protocol (Roche, 11732650001). To prepare for sequencing amplified DNA was gel purified using QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Cell Biology 2021Quote: ... as well as by quantitative PCR (KAPA Biosystems, Sigma-aldrich, St. Louis MA). The sequencing libraries were clustered on a single lane of a flowcell ...
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bioRxiv - Evolutionary Biology 2020Quote: All pre-PCR libraries were amplified using KAPA HiFi Hotstart Uracil+ (Kapa Biosystems), according to manufacturer’s instructions using Illumina single indexing primers ...
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bioRxiv - Microbiology 2021Quote: ... More in detail 22 μl of premix FastStart High Fidelity PCR System (Roche) and 2μl forward and reverse primers ...
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bioRxiv - Microbiology 2021Quote: ... All PCR reactions were performed using KAPA HiFi HotStart ReadyMix (Roche cat # KK2601). Cloning was performed using the NEBuilder HiFi DNA Assembly kit (NEB ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... qRT-PCR was carried out on Light Cycler® 480 Instrument II (Roche). Data were processed by the delta-delta Ct method and normalized per GAPDH as a housekeeping gene ...
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bioRxiv - Microbiology 2021Quote: ... Samples were prepared for PCR with Kapa HiFi HotStart ReadyMix (Roche, Cat # 07958935001) using custom primers ordered from Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... The initial PCR consisted of 2X KAPA HiFi HotStart Ready Mix Taq (Roche), 10 μM total for each forward and reverse primer combination ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were collected and PCR was performed using KAPA HiFi HotStart ReadyMix (Roche) to amplify the targeted genomic region ...
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bioRxiv - Cell Biology 2021Quote: ... PCR was carried out in 384-well LightCycler 480 plates (Roche, Basel, Switzerland) in a LightCycler 480 thermocycler (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... All qRT-PCR reactions were performed in a Roche LightCycler 480 II (Roche Sequencing and Life Sciences ...
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bioRxiv - Cancer Biology 2022Quote: ... qRT-PCR was performed with Fast Start universal SYBR green master mix (Roche) along with gene specific primers on a 7900HT real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix, Roche) from the 2xStrep-tagged or 3xFLAG-tagged plasmids with oligonucleotide primers containing attB recombination sites and recombined into pDONR221 using BP clonase II (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: Lineage targets were PCR amplified using the KAPA HiFi HotStart ReadyMix (Roche, KK2602) with primer sequences below and elongation time of 1 minute and an annealing temperature of 65°C ...
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bioRxiv - Bioengineering 2022Quote: All DNA fragments were PCR amplified using KAPA HiFi HotStart ReadyMix (Roche, Switzerland) and purified by NucleoSpin® Gel and PCR Clean-up Kit (Nachery-Nagel ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed using a LightCycler 480 SYBR Green I Master (Roche), and primers (Extended Data Table 2 ...