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Citations for Roche :
3201 - 3250 of 8657 citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... The PCR reaction (20 μL) consisted of 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48 h and resuspended in lysis buffer (50 mM HEPES pH 7.2, 200 mM NaCl, 10% glycerol, 2 mM DTT, and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed using a microfluidizer ...
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bioRxiv - Cancer Biology 2019Quote: ... coli cells containing the overexpressed RNF114 fusion protein were re-suspended in 80 mL of lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 2 Roche protease inhibitor tablets [without EDTA] ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) and protease inhibitor cocktail (Roche) on ice for 20 min ...
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bioRxiv - Genetics 2019Quote: ... Next embryos were washed in blocking solution (100mM maleic acid, 150 mM NaCl pH 7.5, 2% blocking reagent (Roche)) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Cancer Biology 2021Quote: Human tumor samples were collected on different days right after surgery and digested in Hank’s Balanced Salt Solution supplemented with 2 mg/mL collagenase A (Roche), 2.5 U/mL hyaluronidase (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in ice-cold homogenizing buffer (20 mM HEPES pH 7.4, 250 mM sucrose, 2 mM MgCl2, and EDTA-free protease inhibitor cocktail [Roche]), and homogenized on ice by 25 passages through a 26-guage needle fitted to a 1 mL syringe ...
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bioRxiv - Immunology 2020Quote: ... The red blood cell layer was lysed with 2 mL of Red Blood Cell Lysis Buffer (Cat# 11814389001, Roche) in a 15 mL tube ...
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bioRxiv - Biophysics 2020Quote: ... N2A cells were transfected with 1.0 μg of a cDNA construct and 2 μL of X-tremeGENE HP DNA transfection reagent (Roche Diagnostics GmbH ...
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bioRxiv - Cell Biology 2021Quote: To isolate mammary epithelial cells the third and fourth pairs of mammary glands were removed from 4 to 6-week-old s-SHIP–GFP transgenic (Tg11.5kb–GFP) mice, minced with scissors and digested in digestion medium (DMEM/F12, 2 mg ml−1collagenase I (Roche), 5 mg ml−1 insulin (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were isolated by adding SDS lysis buffer (50 mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol) supplemented with protease inhibitor cocktail (PIC; Roche). DNA was sheared by sonication for 10 min at high settings (30 s on ...
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bioRxiv - Cell Biology 2020Quote: ... were resuspended in 1x sample buffer (0.5 x TBE, 10% glycerol, 2% SDS) supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail (5 mM sodium fluoride ...
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bioRxiv - Plant Biology 2020Quote: ... Cell pellets from 200 mL of Chlamydomonas culture were resuspended in an extraction buffer (20 mM HEPES pH= 7.5, 20 mM KCl, 10% glycerol, 2× EDTA-free protease inhibitor mix (ROCHE)) ...
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bioRxiv - Cell Biology 2020Quote: ... 500 mM Tris pH 7.4, 20 mM EDTA, 10 mM NaF, 2 mM benzamidin and protease inhibitor cocktail [Roche]), and centrifuged 3 min at 5000 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... Peels were cut in 2-5 mm2 pieces and placed in 5 ml digestion solution (0.75 mg/ml Liberase TH Research grade (Roche), 0.1 mg/ml DNAseI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... the small intestines were cut into 2-5mm2 pieces and put into digestion solution (0.75mg/ml Liberase TH Research Grade (Roche), 12 U/ml Dispase ...
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bioRxiv - Biophysics 2022Quote: ... For cell lysis the cells were resuspended in 4 mL per g pellet in lysis buffer (20 mM Tris/HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, Protease Inhibitor (ROCHE), spatula DNAseI ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor tissues were manually minced and digested in 25 mL of Dulbecco’s Modified Eagle Medium (DMEM) containing 2 mg/mL of collagenase A (Roche) and DNase I (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... before treating with lysis buffer (10 mM Tris pH 7.5, 2 mM EDTA) containing a protease and phosphatase inhibitor cocktail (Roche). Chromatin-enriched protein fractions were prepared essentially as described (Mailand and Diffley ...
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bioRxiv - Neuroscience 2021Quote: Proteins were extracted from one brain hemisphere or from purified MVs in 2% SDS (500 µl or 50µl per sample, respectively) with EDTA-free Complete Protease Inhibitor (Roche), sonicated three times at 20 Hz (Vibra cell VCX130 ...
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bioRxiv - Biophysics 2021Quote: ... Platelet activation was achieved by complementing the Tyrodes buffer with 2 mM Ca2+ and the addition of 0.01 U/ml Thrombin (10602400001, Roche) to the platelet suspension.
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bioRxiv - Neuroscience 2022Quote: ... were mixed with an equal volume of 100 mmol/L Tris-HCl (pH 7.4) – 2% sarkosyl for 30 minutes prior to PK (200 μg/mL; Roche) digestion for 1 h at 37° C ...
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bioRxiv - Microbiology 2022Quote: ... 1.5 million S2 cells were transfected with 2 μg of plasmid using Xtreme-GENE HP DNA transfection reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was diluted 1:5 in PCR-grade dH2O and 1.5 µl of the cDNA-mix was used per 20 µl real-time PCR reaction with 0.25 µM of each primer and 2 x FastStart Essential DNA Green Master Mix (Roche). For each target and reference gene ...
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bioRxiv - Biochemistry 2019Quote: ... washed with PBS and suspended in Ni-wash buffer (20 mM Tris/HCl pH 8, 10 mM imidazole, 200 mM NaCl, 10 mM DTT, 2 mM PMSF, 0.5x Roche Protease Inhibitor cocktail ...
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bioRxiv - Genetics 2021Quote: ... Isolation of cfDNA from 2 mL of plasma was performed using the semi-automated MagNA Pure 24 System (Roche) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2021Quote: ... adjust pH to 9.5 adding HCl) for 2 minutes and then tissue was covered with NBT/BCIP stock solution (Roche) diluted in 0.1M TRIS buffer (pH 9.5 ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]) ...
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bioRxiv - Plant Biology 2021Quote: ... plant tissue was homogenized in 2 ml buffer (100 mM Tris pH8, 150 mM NaCl, 0.5% TX-100, protease inhibitor cocktail (Roche)) per gram of tissue ...
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bioRxiv - Biochemistry 2020Quote: ... cells were lysed in Buffer A (2 M NaCl, 20 mM HEPES pH 7.5, 20 mM Imidazole, 5 mM βME, protease inhibitor (Roche)) by sonication ...
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bioRxiv - Genomics 2019Quote: ... 12000g and supernatant diluted with 1 volume of Dilution Buffer (1mM EGTA 300 mM NaCl, 2% Triton x-100, 0.2% sodium deoxycholate, 1x protease inhibitors-Roche). Sonicated chromatin was then incubated 4h at 4°C on a rotating wheel with 6 ug of antibody conjugated to 20 µl of Dynabeads ...
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bioRxiv - Cell Biology 2019Quote: ... 50 mM Tris/HCl pH 7.4/ 50 mM NaF, 10 mM Na4P2O7, 2 mM MgCl2 and Complete Mini, EDTA-free protease inhibitor [Roche]). Lysates were cleared and incubated with GFP-Trap agarose beads for 60 min ...
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bioRxiv - Neuroscience 2020Quote: ... The beads were then washed 2 × with washing buffer (20 mM Tris, 150 mM NaCl, 0.5 % Triton-X-100, 2 mM EGTA, complete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Physiology 2021Quote: ... Muscles were homogenized in buffer (2% sodium dodecyl sulfate, 50□mM Tris-HCl, pH 6.8) with protease and phosphatase inhibitors (Roche). Protein concentrations were determined by Bicinchoninic acid assay (Sigma-Aldrich ...
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bioRxiv - Physiology 2020Quote: ... were sonicated in lysis buffer (2% SDS, 60 mM Tris-HCl, pH 6.8) with phosphatase and protease inhibitors (Roche). Protein concentration was measured using a BCA assay (Pierce) ...
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bioRxiv - Biochemistry 2021Quote: Harvested cells were resuspended in lysis buffer (25 mM Tris pH 8.0, 150 mM NaCl, 2 μM Erlotinib and cOmplete Protease Inhibitor Cocktail (Roche)) and were lysed by sonication in pulse mode for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and sonicated in melanin buffer (50 mM Tris, 2 mM EDTA, and 150 mM NaCl, pH 7.4) supplemented with protease inhibitor cocktail (Roche). Insoluble material was pelleted for 15 min at 16,000 g (4°C) ...
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bioRxiv - Developmental Biology 2020Quote: ... washed three times 15 min each in PBST and pre-incubated for 1h at 60°C in Hybridization Buffer (HB: 50% Formamide, 2X SSC, 1mg/ml Torula RNA, 0.05mg/ml, Heparin, 2% Roche blocking reagent ...
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bioRxiv - Developmental Biology 2020Quote: ... the tissue was minced and enzymatically digested in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001), 100 U/ml Hyaluronidase (Sigma #H3506) ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
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bioRxiv - Developmental Biology 2022Quote: ... DIG-labelled cDNA probe was incubated at 95 °C for 10 min and immediately placed in the ice for 5 min before adding to the hybridization buffer (5x SSC, 50 % formamide, 0.02 % SDS, 2 % blocking agent (Roche), DEPC-treated water) ...
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl containing a ~2-fold molar excess of FimC and complete EDTA-free protease inhibitor cocktail (Roche). The solution was immediately desalted and the buffer exchanged by passing it over a Sephadex G25 (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... disulfide-bonded FimI at 4 °C 30-fold into 20 mM acetic acid-NaOH pH 5.0 containing a ~2-fold molar excess of FimC and complete EDTA-free protease inhibitor cocktail (Roche). FimCI was then dialyzed against 20 mM MOPS-NaOH pH 6.7 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were lysed in lysis buffer (2% CHAPS, 50 mM HEPES, pH 7.4, 150 mM NaCl, 1mM Na3VO4, 1X EDTA-free PIC (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell pellet was dissolved in lysis buffer 2 (1x HEMG with 200 mM NaCl, 0.5% NP-40, 1x protease inhibitor cocktail (Roche), 0.5mM PMSF ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were washed with 2 X SSC and 0.2 X SSC for 20 min followed by incubation in a blocking solution containing 2% blocking reagent (Roche) and 20% sheep serum for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... finely chopped and incubated for 2 hours in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were harvested by cell scraping into 2 ml of PBS with a protease inhibitor cocktail (Roche cOmplete 4693159001). The cell suspension was centrifuge at 200xg for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in 20 mM Tris-HCl (pH 7.4) with 2% SDS supplemented with a proteinase inhibitor (cOmpleteTM, EDTA-free Protease Inhibitor Cocktail, Roche). Protein concentration of lysates was measured using a bicinchoninic acid (BCA ...