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Citations for Roche :
251 - 300 of 700 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared in radio-immunoprecipitation assay (RIPA) buffer supplemented with PhosSTOP and complete protease inhibitor cocktail (Roche). Protein concentration was determined by Bradford assay using the Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Viability of the tissue was assessed by an LDH Cytotoxicity Detection Kit (Roche) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... Tissues were minced in RPMI complete medium containing collagenase (1 mg/mL, Roche), DNase I (100 μg/mL ...
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bioRxiv - Immunology 2022Quote: Tissues were homogenized in 600 µL of PBS using a MagNA Lyser (Roche), then 150 µL of homogenate was added to an equal volume of buffer RLT (Qiagen ...
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bioRxiv - Pathology 2020Quote: ... the tissue sections were incubated with a universal secondary antibody (Roche Diagnostics KK) for 20 min at 37 °C and then visualized by the DAB Map detection kit (Roche Diagnostics KK) ...
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bioRxiv - Neuroscience 2022Quote: ... CGE and cortex tissue was incubated in 1 mg/mL Pronase (Roche #10165921001) in ACSF for 20 minutes at RT ...
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bioRxiv - Immunology 2019Quote: ... Colonic tissues were digested at 37°C for overnight with Collagenase A (Roche) and DNase I (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg α-AGO1 antibody (Agrisera)/g leaf tissue or 0.1 μg α-HA antibody (3F10, Roche or 12CA5)/g leaf tissue were used ...
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bioRxiv - Developmental Biology 2020Quote: ... minced tissues were transferred to a digestion solution containing 3mg/mL collagenase (Roche), 100 units/mL hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... the tissues were counter stained in DAPI (5μg/ml, Roche Diagnostics GmbH, Germany) and mounted in Prolong Gold antifade reagent (Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Intestinal tissue was digested in media containing 0.25 mg/mL liberase TL (Roche) and 10 U/mL RNase-free DNaseI (Roche ...
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bioRxiv - Immunology 2020Quote: ... Tissues were then transferred to a solution of 0.5mg/ml Collagenase D (Roche) and 15μg/ml DNase I (Roche ...
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bioRxiv - Bioengineering 2022Quote: ... we digested the tissue with 0.2% collagenase-P (Roche Pharmaceuticals, Cat. No. 11213873001) to harvest and culture primary chondrocytes ...
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bioRxiv - Neuroscience 2022Quote: ... Small tissue pieces were digested with 0.5 mg/ml collagenase D (Roche 11088882001) and 5 U/ml DNAse 1 (Sigma-Aldrich 10104159001 ...
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bioRxiv - Microbiology 2023Quote: ... and PCR products were purified using the DNeasy blood and tissue kit (Roche), the High Pure Plasmid Isolation kit (Roche) ...
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bioRxiv - Physiology 2023Quote: ... Tissue samples were homogenized in cell signaling lysis buffer containing protease inhibitors (Roche) and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Tissue was digested in RPMI 1640 containing 1.25 mg/ml collagenase D (Roche), 0.2 mg/ml of Dispase II (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... brain tissue was homogenized in PBS containing 1x protease inhibitor cocktail (05892970001, Roche). Protein concentration was measured using the Bicinchoninic Acid (BCA ...
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bioRxiv - Immunology 2024Quote: ... Solid tissues were mechanically dissociated following enzymatic treatment with either Liberase TL (Roche) or collagenase IV (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested into single cells (tissues) using a mix of collagenase/dispase (Roche), accutase (Millipore) ...
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bioRxiv - Neuroscience 2024Quote: ... remaining tissue was lysed and gDNA amplified by means of PCR (KAPA Biosystems) as described above ...
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bioRxiv - Cell Biology 2020Quote: ... or His6 (full length mouse MLKL and pseudokinase domains) tags and captured from lysates using glutathione resin (UBP Bio) or HisTag Ni-NTA resin (Roche) respectively ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of the post-nuclear supernatant was saved as the input lysate and 450 μL was incubated with 5μg of anti-GFP antibody (Roche 11814460001) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... cell lysates were retrieved in 1% sodium deoxycholate (SDC) in phosphate buffered saline solution with Protease Inhibitor Cocktail (cOmplete, Roche) buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were prepared as previously described (Plate et al., 2016) in RIPA buffer with fresh protease inhibitor cocktail (Roche). Total protein concentration in cellular lysates was normalized using the Bio-Rad protein assay ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ml of the lysates prepared with IP extracation buffer from 0.5 g of leaves was incubated with anti-HA Affinity Matrix (Roche 11815016) for o/n in a rotary shaker at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: Cell lysates were prepared in 2× Laemmli loading buffer supplemented with cOmplete protease inhibitor and PhosSTOP phosphatase inhibitor tablets (Roche), 1 mM PMSF ...
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bioRxiv - Microbiology 2020Quote: Whole cell lysates were obtained by lysing cells in RIPA buffer supplemented with a cOmplete Mini protease inhibitor cocktail (Roche). Protein concentrations were measured using a BCA assay (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant human Sox6 HMG domain was purified from supernatant of bacterial lysate by using NiNTA resin (Complete His-Tag purification Resin, Roche) and followed by SP sepharose column chromatography (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Whole-cell lysates were prepared by using Triton-X sample buffer containing protease inhibitor cocktail from Roche (Grenzach-Wyhlen, Germany). The protein concentration was assessed by using the DC Protein assay reagent obtained from Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5% glycerol with the addition of 1 EDTA-free cOmplete protease inhibitor cocktail tablet per 50 mL lysate (Roche). Cells were lysed by sonication on ice with stirring and the lysates were clarified by centrifugation at 20,000 g for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Protein lysates were prepared following euthanasia of zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Complete ULTRA Tablets, Roche), followed by homogenisation via probe sonication ...
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bioRxiv - Cell Biology 2019Quote: Protein lysates from whole pancreas were prepared in 1 mL of RIPA buffer supplemented with phosSTOP phosphatase and protease inhibitors (Roche). Protein concentrations were measured using the BCA kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed pellets were resuspended with 200-300 µL native lysate buffer (1% NP-40 with 1X cOmplete protease inhibitor cocktail [SKU, 11836145001, Roche] ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates were collected from worms using RIPA buffer (50 mM Tris-HCl, 250 mM sucrose, 1 mM EDTA, and Roche protease inhibitor tablet ...
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bioRxiv - Genomics 2022Quote: ... whole transcriptome mRNA-enriched RNA-Seq libraries were constructed from cell lysates using a KAPA mRNA Capture Kit and mRNA HyperPrep Kit (Roche) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... eIF2αS/S and eIF2αA/A MEFs transduced with TauRD-YFP and Biosensor cell transfected with wild-type and PS19 brain lysates were lysed with SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (11836153001, Roche)) or RIPA buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were incubated with Protein A/G-Agarose beads (Cat# 11 134 515 001/ 11 243 233 001, Roche), and either ARP3 or normal Rabbit IgG antibody for 2 h at 4°C with rotating ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were centrifuged at 10,000 x g for 15 minutes to pellet and remove insoluble material after which the remaining soluble lysate was incubated with anti-HA sepharose beads (Roche) for 4 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C), the lysate (IN, input) was transferred to either 50 µl of HA bead slurry (anti-HA affinity matrix, Roche) or 50 µl c-myc bead slurry (EZview red anti-c-myc affinity gel ...
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bioRxiv - Immunology 2023Quote: ... 0.5 –1 L of the crude lysates were treated with 5 mg/mL each of DNase I and RNAse (Roche) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Whole cell lysate proteins were extracted using RIPA buffer (Cat. #89901, Pierce) containing complete protease inhibitor cocktails (Cat. #04693116001, Roche) and phosSTOP (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... For Salm protein purification from bacterial lysates (lisozima 0,5mg/mL, Imidazol 10mM and protease inhibitors cOmplete™ Protease Inhibitor Cocktail tablets from Roche) we used 3 rounds of 3 minutes sonication with 15 s on/off cycles ...
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bioRxiv - Neuroscience 2023Quote: Lysates were prepared from 10 homogenised adult or pharate brains in lysis buffer with a phosphatase–protease inhibitor cocktail (Roche) on ice and suspended in LDS sample buffer and reducing agent (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: Lysates from crosslinked cells were prepared by adding 1ml of 1x PXL lysis buffer + quenchers + protease inhibitors (Roche, cat. #11873580001) and triturating to disrupt cells ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted 1:10 in water and qPCR was performed with 3 μL of each diluted cell lysate using the LightCycler 480 Probes Master mix (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Wild-type HMC3 cells and potential G6PD deficient colonies were used to make cells lysate using RIPA buffer supplemented with 1x Protease inhibitor cocktail (Roche). Protein estimation was performed using BCA method as per the manufacturing protocol (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using a 1% triton X-100 containing lysis buffer (CST) supplemented with protease inhibitor cocktail (Roche) on ice for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...