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Citations for Roche :
101 - 150 of 754 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates were prepared by lysing in RIPA buffer with protease inhibitor cocktail (Roche) and protein concentrations were normalized using a BCA assay (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were diluted with 500uL of RIPA buffer with protease inhibitor cocktail (Roche) and subjected to immunoprecipitation with G1 anti-DYKDDDDK affinity resin overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates were prepared with Radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor cocktail (Roche). Proteins were separated by SDS–polyacrylamide gel electrophoresis and electro-transferred onto polyinylidene difluoride membranes which then were subsequently blocked in 5% defatted milk in Tris-buffered saline-0.1 % Tween 20 (TBS- T ...
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bioRxiv - Neuroscience 2020Quote: Cells from mouse brain or spleen tissues were lysed with T-PER Tissue Protein Extraction Reagent (Thermo) containing Complete Protease Inhibitor Cocktail (Roche Applied Science). Protein concentrations were determined with BCA protein assay reagents (Pierce) ...
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bioRxiv - Cancer Biology 2019Quote: ... Tissues were counterstained with Hematoxylin II (Roche, 790-228) for 12 min and Bluing reagent (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissue is counterstained with Red Counterstain II (Roche), followed by dehydration and mounting using Eukitt quick-hardening mounting medium (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... Tissues were homogenized using a MagNA Lyser instrument (Roche).
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bioRxiv - Neuroscience 2022Quote: ... DRG tissues were incubated in 5% Collagenase A (Roche) at 37°C for 70mins and in 0.25% Trypsin (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... in tissues was done by LightCycler PCR (Roche Diagnostics) as previously described ...
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bioRxiv - Microbiology 2022Quote: ... tissues were processed using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760-159). Specific immunoreactivity was detected using the GenScript U864YFA140-4/CB2093 NP-1 SARS-CoV-2-specific antiserum at a 1:1000 dilution ...
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bioRxiv - Physiology 2021Quote: Starting with frozen tissue, tissue was homogenized in homogenization buffer (100 mM PMSF, 500 mM EDTA, EDTA-free Protease Inhibitor Cocktail (Roche, catalog number 11836170001), PBS ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were made in RIPA-buffer supplemented with Protease inhibitor cocktail (11836170001, Roche), 1mM NaF and 1mM NaVO3 ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were incubated with 30 μl pre-washed rat anti-HA Affinity Matrix (Roche) for 3 h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... protein lysates were prepared in RIPA buffer supplemented with complete protease inhibitor (Roche Applied Science). Proteins were then separated on 6%–15% SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2021Quote: Lysate destined for Ribo-seq was incubated with 1500 units of micrococcal nuclease (Roche 10107921001) and 6 uL of SUPERase-In at 25 C for 1 hour and shaken at 1400 rpm ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates were prepared in RIPA buffer with protease and phosphatase inhibitor cocktails (Roche). Lysates were subjected to SDS PAGE electrophoresis and transferred onto PDVF membrane (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: Whole-cell lysates were obtained using RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). An amount of 10 µg of protein was then resolved on 10-12.5% polyacrylamide gels and transferred to nitrocellulose membranes using the semi-dry technique ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates sampled with phospho-specific antibodies were also lysed with phosphatase inhibitors (phosSTOP™, Roche). Lysates were then centrifuged at 20,000 g ...
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bioRxiv - Biochemistry 2019Quote: ... Clear lysate was affinity-purified by incubating it with cOmplete His-Tag purification resin (Roche) for 2 hours (4°C) ...
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bioRxiv - Microbiology 2022Quote: Whole-cell lysates were prepared using Triton-X sample buffer containing protease inhibitor cocktail (Roche). The protein concentration was assessed by using DC Protein assay reagent (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2024Quote: ... The remaining lysate was rotated overnight with 50 µl of Protein A-agarose beads (Roche) and 2 µg of mouse anti-HA (monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... C2C12 cell lysates were prepared in RIPA buffer containing protease inhibitors (Roche Diagnostics, Indianapolis, IN). To examine mitochondrial-associated proteins ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... The cell lysate was pre-cleaned with protein G agarose beads (Roche Diagnostic, Basel, Switzerland) for 1 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: Whole-cell lysates were prepared using Triton-X sample buffer containing protease inhibitor cocktail (Roche). The protein concentration was assessed by using DC Protein assay reagent (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2022Quote: ... Remaining lysate was rotated O/N with 25 µl of Protein-A-agarose beads (Roche) and 2 µg of mouse anti-HA antibody (Supplementary Table S1) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the clarified cell lysates were digested with micrococcal nuclease from Staphylococcus aureus (MNase, Roche, USA) for 1 hour at 25 °C under continuous agitation ...
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bioRxiv - Microbiology 2022Quote: ... IHC tissues were processed using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760-159) for immunohistochemistry (IHC ...
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bioRxiv - Genetics 2020Quote: ... or placenta tissues was homogenised using the MagNA Lyser (Roche) at 6,000 x g for 40 s ...
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bioRxiv - Cancer Biology 2019Quote: ... pancreatic tissue was minced and digested with 0.02% Pronase (Roche), 0.05% Collagenase P (Roche) ...
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bioRxiv - Immunology 2019Quote: ... Tissue pieces were then digested using 0.3mg Liberase TM (Roche) and 0.15 mg of DNase (Sigma Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Tissues were digested with Liberase TL (100 μg/mL, Roche) and DNaseI (100 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue was incubated with anti-FITC-POD (1:2000; Roche) or anti-DIG-POD (1:1000 ...
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bioRxiv - Genetics 2019Quote: Tissue was collected to MagNA-Lyser Green Beads Tubes (Roche) and RNA was extracted using SIGMA-ALDRICH Spectrum Plant Total RNA kit ...
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bioRxiv - Immunology 2021Quote: Tissues were collected in PBS supplied with protease inhibitors (Roche). Samples were homogenized ...
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bioRxiv - Developmental Biology 2020Quote: ... Fixed tissues were stained with X-gal solution (Roche 11828673001) for 22 hours at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... on the Ventana Discovery ULTRA staining platform (Roche Tissue Diagnostics). A board-certified veterinary anatomic pathologist blinded to the study groups evaluated all tissue slides.
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bioRxiv - Cancer Biology 2022Quote: Samples of total RNA extracted from FFPE tumor tissue (ROCHE High Pure FFPE kit ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were thawed and homogenized using a MagNA Lyser (Roche) set to 6000 for 1 min ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was dissociated in papain (15 U/mL, Roche) containing 1 mM L-cystein ...
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bioRxiv - Cell Biology 2023Quote: ... Liver tissue was digested with pronase and collagenase B (Roche) and the cell suspension was subsequently separated by an 11.5% Optiprep gradient (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Liver tissue was digested with pronase and collagenase B (Roche) and the cell suspension was subsequently separated by an 11.5% Optiprep gradient (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... The tissue was incubated overnight in 0.2% dispase II (Roche) in DMEM at 4°C ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted using High Pure RNA Tissue Kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Tissue was digested with 0.5 mg/ml DNAse I (Roche) and 0.25 mg/ml Liberase TM (Roche ...
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bioRxiv - Immunology 2021Quote: Splenocytes lysates were prepared using Cell Lysis Buffer (#9803, CellSignaling Technology) supplemented with protease inhibitors (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
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bioRxiv - Genetics 2019Quote: ... Lysates were diluted in RIPA buffer with 1 µM PMSF and Complete Protease Inhibitor Cocktail (Roche) to a concentration of 1.68 mg/ml and 500 µl was used for capture of biotinylated proteins on streptavidin beads and subsequent mass spectrometry analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (0.5 mg) were incubated with anti-HA antibody-coupled agarose beads (Roche, Basel, Switzerland) for 16 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...