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Citations for Roche :
2801 - 2850 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and protein was extracted using SDS lysis buffer93 with cOmplete protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation (IP) of HA-tagged proteins was performed using Anti-HA Affinity matrix (Roche) under denaturing conditions ...
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bioRxiv - Cell Biology 2023Quote: ... blocked in 5% nonfat dry milk and proteins detected using monoclonal anti-GFP (Roche), monoclonal anti-PGK (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Secreted IgGs were purified from cell supernatants with Protein A resin (Roche, Basel, Switzerland). IgGs were eluted at low pH using Protein A elution buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with 20 μL of resuspended volume of Protein G beads (Roche) for 2 h at 4 °C to pull down bound proteins ...
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bioRxiv - Biochemistry 2024Quote: Protein extracts were prepared using RIPA buffer supplemented with protease inhibitors (Complete ULTRA, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were purified by affinity on cOmplete™ His-Tag Purification Resin (Roche®). Columns were washed with Buffer A followed by washing with 50 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins in each sample were digested with 20 mg of Proteinase K (Roche 3115879001) for 1.5 hours at 37°C ...
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bioRxiv - Cell Biology 2024Quote: Protein lysates were prepared in the following lysis buffer supplemented with protease (Roche, 11836153001) and phosphatase (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... albumin and total protein were measured using a Cobas 8000 chemistry analyser (Roche Diagnostics). Concentrations of lactate (L and D) ...
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bioRxiv - Biochemistry 2020Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 1 mM sodium orthovanadate, 1 mM PMSF and Roche’s protease inhibitor tablet (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% P/S and 1 mg ml-1 collagenase A (Roche) for 90 minutes ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were disrupted by sonication in a lysis buffer containing 10 mM Tris-HCl pH 7.4 (buffer A) and Complete Protease Inhibitor Cocktail (Roche). Cell debris was removed by centrifugation at 16,000 g at 4°C during 10 minutes and supernatants were collected as total extracts ...
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bioRxiv - Cell Biology 2020Quote: ... and the pellet resuspended in 100 μl of ice-cold RIPA lysis buffer containing 1x protease inhibitor cocktail (Roche), incubated for 30 min on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... tissues were dissected in ice cold 1x PBS and transferred to low binding PCR tubes containing 50-100µL of 1x Protease Inhibitor Cocktail in 1xPBS (cOmplete mini EDTA-free; Roche) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... The cell pellet collected from 6 L growth was resuspended in 20 mM HEPES (pH 7.8) containing protease inhibitor cocktail (Roche) and DNase I (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated visual cortices from Ppt1-/- and WT animals were homogenized in ice-cold synaptosome buffer (320mM sucrose, 1mM EDTA, 4mM HEPES, pH7.4 containing 1x protease inhibitor cocktail (Roche), 1x phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... 1.5 mM, 10% glycerol, pH 7.4, containing phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche). Protein concentration was adjusted to 10 mg/ml by the Bradford method (Pierce ...
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bioRxiv - Immunology 2022Quote: ... tissues were minced and placed into gentleMACS C tubes (Miltenyi 130-093-237) containing 15 mL enzyme digestion solution (10 mM HEPES, 0.2 U/mL Liberase TM [Roche 5401127001] ...
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bioRxiv - Genomics 2020Quote: ... the pellets were washed with ice-cold nuclei suspension buffer (1X PBS containing 2% BSA and 0.2 U/µl Protector RNase Inhibitor, ROCHE) and filtered through a 30μm cell strainer (Fisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... 5 ul of the three amplicons with 20 μl of PCR master mix containing 1.25X KAPA HiFi HotStart ReadyMix (Roche), 0.375 uM of the i5 primer (Microsynth AG ...
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bioRxiv - Systems Biology 2020Quote: ... Supernatants were discarded and protein resuspended in Guanidine-HCl buffer (6M Gnd-HCl, 50mM Tris-HCl, pH 8.5, 5mM NaF, 5mM beta-glycerophosphate, 1mM Na-orthovanadate, containing Roche complete protease inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... and pellets were disrupted by sonication in PBS buffer (pH 8.0) containing 10 mM EDTA as well as protease inhibitors (Roche) and ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Neuroscience 2021Quote: ... samples were homogenized in a lysis buffer containing a mixture of protease inhibitor (Protease Inhibitor Cocktail Tablets - Roche Diagnostics). Proteins were separated using SDS-polyacrylamide gel electrophoresis (SDS-PAGE-4-12% ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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Biochemical alterations precede neurobehavioral deficits in a novel mouse model of Friedreich ataxiabioRxiv - Biochemistry 2021Quote: ... All the pieces were placed in 1.5 ml screw cap polypropylene tubes in the presence of lysis buffer consisting in 50 mM tris(hydroxymethyl)aminomethane (Tris) HCl pH 7.5 containing a protease inhibitor cocktail (Roche). For each 100 mg of tissue ...
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bioRxiv - Biochemistry 2020Quote: ... Elution buffer (50 mM Na2HPO4, 0.3 M NaCl, 250 mM imidazole; pH 8) containing EDTA-free protease inhibitors (cOmplete, Roche) was used to elute the proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Lacrimal glands were also harvested and homogenized in 0.2% Triton X-100 (MilliporeSigma) containing a protease inhibitor cocktail (Roche) and prepared for PRG4 western blotting (2.2) ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were lysed with 400 μL of NAD+/NADH extraction buffer containing a cocktail of protease inhibitor (Roche Diagnostic ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed in 1% NP-40 lysis buffer (150 mM NaCl, 20 mM HEPES, 0.5 mM EDTA) containing complete protease and phosphatase inhibitor cocktail (Roche). Total cell extracts were incubated for 3 h with 20 μl protein A Dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The cells pellet was resuspended in Lysis buffer (20mM Tris-HCl pH 7.5, 150mM NaCl, 1mM EDTA) containing protease inhibitor cocktail (Roche) and lysed using mechanical disruption (Isobiotec Cell Homogenizer) ...
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bioRxiv - Cell Biology 2021Quote: ... then 10-15 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) containing 0.4 mg/ml Protease (Roche) and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... 300ul lysis buffer (50mM Tris-HCl, 150mM NaCl, 0.5% NP40 and 1mM EDTA) containing protease inhibitor (Roche, Basel, Switzerland) and 0.2M PMSF was added to each well and incubated on ice for 15 minutes ...
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bioRxiv - Neuroscience 2020Quote: Retinas were lysed on ice for 10 min in lysis buffer (20mM HEPES, 150mM NaCl, 1.5mM NgCl2, 2mM EGTA with 0.5% TritonX-100) containing protease inhibitors (Roche #5892970001) and phosphatase inhibitors (10mM NaF ...
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bioRxiv - Microbiology 2021Quote: The AAV2 stock sample (1.5*1011 genome-containing particles) was pre-treated with 10U DNase I (04716728001, Roche, Switzerland) in 1x DNase buffer for 15 min at 37°C to remove any DNA outside the particles ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cultures were centrifuged at 700 g for 3 min to remove the media and parasites were isolated from the RBC by incubating with 0.1% saponin in PBS containing protease (Roche) and phosphatase inhibitors (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from 200 µl of virus-containing cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in 10 μl reactions containing 4 μl of LightCycler 480 SYBR Green I Master (Roche), 4 μl of PCR-grade water (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were treated in lysis buffer (50 mM Tris-HCl, 0.5% sodium deoxycholate, 25 mM EDTA) containing protease inhibitor cocktail (Roche) for 30 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... cells from mouse blood infected with the DEH-GFP-expressing parasite were pelleted and then lysed in hypotonic buffer (10 mM Tris-HCl pH 8.4, 5 mM EDTA) containing protease inhibitors (Roche), freeze/thawed twice ...
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bioRxiv - Cell Biology 2021Quote: ... were harvested in ice-cold lysis buffer (0,25% NP40, 5% glycerol, 50 mM Tris HCl, 150 mM NaCl) containing protease inhibitor (Roche), DNAse (5 µl/ml ...
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bioRxiv - Cell Biology 2021Quote: ... The pelleted cells were homogenized in 1X RIPA lysis buffer containing freshly added phosphatase and protease inhibitor cocktail (Roche). To remove cellular debris ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were injected in multiple locations with a total of 1.5 mL RPMI containing 50 µg/mL Liberase DL research grade (Roche) and 10 U/mL DNase I (Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... 0.3 M NaCl) containing either 200 µM PMSF or a tablet of complete EDTA-free protease-inhibitor cocktail (Roche) and 10 ng ml−1 DNAse I ...
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bioRxiv - Neuroscience 2022Quote: ... The human tissues were homogenized in TBS (20 mM Tris-HCl, 140 mM NaCl, pH 7.5) containing protease and phosphatase inhibitors (Roche). Homogenates were ultracentrifuged (4 °C for 60 min ...
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bioRxiv - Developmental Biology 2022Quote: ... The alkaline phosphatase activity was visualized by incubating the specimens in NTMT containing 0.07 mg/ml nitroblue-tetrazolium chloride (Roche), and 0.035 mg/ml 5-bromo-4-chloro-3-indolyphosphatase (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... after which the cultures were treated with 0.15% saponin in PBS containing cOmplete Mini EDTA-free Protease and PhosSTOP Phosphatase inhibitor cocktails (both Roche) for 10 min at 4°C to lyse the host erythrocytes.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lung segments were suspended in 10X volume of ice cold PBS containing cOmplete Mini protease inhibitor cocktail (Roche, Switzerland), 2mM EDTA (Sigma ...
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bioRxiv - Physiology 2022Quote: ... for 3 min and incubated twice for 3 min with CSK buffer containing 0.5% Triton X-100 and complete protease inhibitors cocktail (catalog no. 11873580001, Roche). Cells were after washed with PBS-S ...