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Citations for Roche :
2601 - 2650 of 8008 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using SYBR Green qPCR Master Mix (TOYOBO, QST-100) in the LightCycler480 system (Roche). The constitutively expressed tubulin gene (MGG_00604 ...
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bioRxiv - Cell Biology 2024Quote: ... and the fixed cells were then resuspended in 1 ml RT vPBS containing EDTA-free protease inhibitor (Roche). The cells were attached to the coverslips by centrifugation (1000 x g ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed according to protocols provided by the manufacturer using FastStart Essential DNA Green Master (Roche) and LightCycler96 (Roche ...
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bioRxiv - Immunology 2020Quote: ... The membrane was washed three times with NBT/BCIP (Roche Diagnostics, Mannheim, Germany) before being incubated with alkaline phosphatase-labeled anti-rabbit IgG (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: The different CRE candidate sequences were amplified using PCR-specific primers (see Table S2) and the Expand High Fidelity PCR system (Roche). Amplified fragments were gel-purified and cloned into the pSK-lacZ vector as in (Beccari et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Primers were generated by www.leishgedit.net (Table S5) and all PCR reactions were performed using Expand High Fidelity PCR System (Roche, Basel, Switzerland), pooled and purified using PCR purification kits ...
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bioRxiv - Plant Biology 2019Quote: ... PCR was performed using 96-well white PCR plates using the LightCycler® 480 II system (Roche Applied Science, Germany). The amplification started with an initial denaturation step at 95°C for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: Four consecutive and non-overlapping fragments from the pSP64 HPV16 bacterial artificial chromosome were PCR amplified using the Expand High Fidelity PCR system (Roche). This resulted in complete coverage of the HPV16 genome ...
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bioRxiv - Neuroscience 2022Quote: ... The attB-flanked hFGD4 fragment was produced by PCR using the “Expand High Fidelity Plus PCR System” (#3300226001, Roche, Switzerland) and the following primers ...
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bioRxiv - Systems Biology 2022Quote: ... PCR was immediately performed by adding 6ul of PCR mix to each well (2ul Kapa HiFi Hotstart buffer (5x, Roche), dNTPs 0.12ul (25mM/each ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We generated standard PCR-free Illumina paired-end sequencing libraries using KAPA Hyper PCR-free library reagents (KK8505, KAPA Biosystems) in Beckman robotic workstations (Biomek FX and FXp models) ...
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bioRxiv - Genetics 2019Quote: ... The PCR reactions were performed on the Access Array System (Fluidigm) using KAPA2G 5X Fast Multiplex PCR Mix (Kapa Biosystems). Barcodes were added in a second round of PCR using Phusion DNA polymerase (NEB cat ...
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bioRxiv - Cancer Biology 2020Quote: A unique Fluidigm barcode was added to each sample by PCR in 10 μl reactions using the Fast Start High Fidelity PCR System (Roche) containing ...
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bioRxiv - Biochemistry 2019Quote: ... the collected cDNA solution was used for qRT-PCR using Power SYBR® Green PCR Master Mix (Roche, Indianapolis, USA) according to the schemes of the manufacturer ...
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bioRxiv - Synthetic Biology 2020Quote: ... Each of these pools was amplified in a 50 μl PCR reaction using Kapa HiFi HotStart PCR Mix (Kapa Biosystems), 40 ng of synthesized oligonucleotide as template and pool-specific primers ...
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bioRxiv - Genomics 2021Quote: ... The oligonucleotides were used as PCR templates and amplified by six rounds of PCR using KAPA HiFi polymerase (Kapa Biosystems) and primers F_N50_lib and R_N50_lib ...
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bioRxiv - Cell Biology 2020Quote: Global amplification of the cDNA by PCR was then performed by adding PCR mastermix consisting of: 12.5ul 2x KAPA HiFi HotStart ReadyMix (KAPA Biosystems), 0.25ul 10mM Smart PCR primer ...
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bioRxiv - Genomics 2021Quote: ... PCR products from the 5 reactions were mixed for library preparation using FastStart High Fidelity PCR System dNTPack (Roche, 3553400001) and Nextera XT Index Kit (illumina ...
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bioRxiv - Microbiology 2022Quote: ... A twenty-fold dilution of the first PCR was used as template for a second PCR with primers Anchor (Roche) and sp3R for czcR cDNA or sp2C for czcC cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... The structure of the lacO–tetO reporter locus was checked by a set of 4 PCRs using Expand Long Template PCR System (Roche) and the following primer pairs ...
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bioRxiv - Molecular Biology 2024Quote: ... The digoxigenin (DIG)-labeled probes were generated by PCR on H26 genomic DNA using primers and the PCR DIG labeling Mix from Roche. Probe hybridization was detected using the DIG Luminescent Detection kit (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We did PCRs in a reaction volume of 25 μl containing 12.5 μl 2x FastStart PCR master mix (Roche, Germany), one forward and two reverse MHC IIB primers (Bracamonte et al. ...
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed in a 20 μL-volume system using Expand High FidelityPLUS PCR System (Roche Diagnostics, Indianapolis, IN, USA) according to Elaswad et al ...
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bioRxiv - Genomics 2022Quote: ... Re-purified circular fragments were amplified by PCR with View-Point specific primers (see Supplementary Table 2) using the Expand Long Template PCR System (Roche). Resulting amplicons were purified with a 0.8× ratio of AMPure XP beads (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... Screening for repeat expansions in RFC1 was achieved by a combination of short-range repeat-spanning end-point PCR (Supplementary Table 1) with 2X Faststart PCR mastermix (Roche) and by Repeat-Primed PCR using 2X Phusion Flash High-Fidelity PCR Mastermix (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... no-chloroplast amplifying primers 335F (CADACTCCTACGGGAGGC) and 769R (ATCCTGTTTGMTMCCCVCRC) (Dorn-In et al. 2015) were used together with KAPA2G Robust PCR Kits (Kapa Biosystems, Cat. No. KK5005, Wilmington, USA). In protocol 2 (P2) ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed using LightCycler 480II (Roche) by a universal probe method ...
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bioRxiv - Cell Biology 2020Quote: ... as well as by quantitative PCR (KAPA Biosystems).
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bioRxiv - Cell Biology 2020Quote: ... Expand High Fidelity PCR System (#11759078001, Roche, Switzerland) rabbit anti HA-tag antibody (#ab9110 ...
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bioRxiv - Developmental Biology 2021Quote: ... as well as by quantitative PCR (KAPA Biosystems). The sequencing libraries were clustered on a single lane of a flowcell ...
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bioRxiv - Molecular Biology 2020Quote: ... PCRs were performed thanks to a LightCycler480 (Roche) using a mix of 1x LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Systems Biology 2020Quote: ... Libraries were PCR amplified using Kapa HIFI (Roche) with 10µM PB5_pr1 and 10µM SplAP1 during 18 cycles of 20sec 98°C ...
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bioRxiv - Systems Biology 2020Quote: ... and quantitative PCR (Kapa Biosystems, Wilmington, MA, USA). 8 pools of 24 randomized libraries were sequenced in 3 lanes at 100 bp single-end on the HiSeq 2500 (Illumina ...
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bioRxiv - Immunology 2020Quote: ... PCR was run on a LightCycler® (Roche) with 2 mins 50°C ...
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bioRxiv - Biochemistry 2020Quote: ... PCR was performed on LightCycler 96 Instrument (Roche) as described previously [36].
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed using SYBR Green (Roche) and a QuantStudio 7 Flex (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed using SYBR Green (Roche) and a QuantStudio 7 Flex (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... as well as by quantitative PCR (KAPA Biosystems).
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bioRxiv - Microbiology 2022Quote: ... as well as by quantitative PCR (KAPA Biosystems). The sequencing libraries were clustered on one lane of a flow cell ...
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bioRxiv - Cancer Biology 2021Quote: ... as well as by quantitative PCR (KAPA Biosystems). The sequencing libraries were clustered on one lane of a flow cell ...
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bioRxiv - Plant Biology 2021Quote: ... 5 μl SYBR Green PCR master mix (Roche) and ultrapure water up to 10 μl was used and analyzed using the LightCycler 480 System (Roche) ...
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bioRxiv - Pathology 2021Quote: ... 5 μl of PCR DIG labelling mix (Roche), 3 μl template DNA ...
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bioRxiv - Cell Biology 2020Quote: ... or 1x Kappa HiFi PCR buffer (Kapa Biosystems). For locally doublestranded cleavage tests pBB3 was added to a final concentration of 3 μM ...
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bioRxiv - Synthetic Biology 2020Quote: PCR was performed using KAPA HiFi (Roche KK2101) for all experiments with the exception of multiplex PCR for screening Yeast Artificial Chromosomes (YACs) ...
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bioRxiv - Genetics 2020Quote: ... Quantitative PCR was performed on a lightcycler (Roche) with SYBR chemistry ...
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bioRxiv - Biochemistry 2021Quote: ... Expand high fidelity PCR system (Roche Life Science) were used for PCR reactions (Bio-Rad,USA) ...
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bioRxiv - Immunology 2021Quote: ... and a Light Cycler 480 PCR system (Roche). All tests were carried out on duplicate reaction mixtures in 96-well plates ...
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bioRxiv - Microbiology 2021Quote: ... as well as by quantitative PCR (KAPA Biosystems). The sequencing libraries were pooled and clustered on 1 lane of a flowcell ...
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bioRxiv - Cell Biology 2022Quote: ... and TaqMan universal PCR master mix from Roche Applied Science ...
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bioRxiv - Cancer Biology 2022Quote: ... and the LightCycler 480 PCR platform (Roche Diagnostics). Ct values above 35 were considered not detected ...