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Citations for Roche :
2401 - 2450 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 20 ng of sample were loaded in addition to the following reagents: LightCycler SBYR green 480 Master Mix (Roche LifeScience, Product No. 04707516001) and the specific primers for the target genes (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA mRNA Hyperprep kit (v4.17) (Kapa Biosystems, Wilmington, MA USA). RNA was sheared to 300-400 bp ...
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bioRxiv - Genomics 2023Quote: ... Bank validation was done with the quantification of the complementary DNA with the Standard Sensitivity NGS kit on Fragment Analyzer and with qPCR (ROCHE Light Cycler 480). The sequencing was done on NovaSeq 6000 (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... 50 ng cDNA were used for each technical triplicate and the PCR reaction was performed in a LightCycler® 480 (Roche, Basel, Switzerland). -RT and water controls for each primer pair were included ...
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bioRxiv - Cancer Biology 2023Quote: ... Total yield of around 40 ng was split in two and PCR amplified using 2X KAPA HiFi Hot-Start ReadyMix (Roche, cat. no. KK2602) and KAPA Library Amplification Primer Mix (10X concentration ...
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bioRxiv - Microbiology 2023Quote: ... 0.09 ng of cDNA per well was used in qRT-PCR with KAPA SYBR® FAST qPCR Master Mix Kit (2×) (KAPA Biosystems, USA) on an Applied StepOnePlusTM Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA was used for NGS library preparation using either the KAPA Library Preparation kit or the KAPAHyperPlus kit (KAPA Biosystems, MA, USA). Genomic libraries were captured with the HyperPlus Capture Exome Kit or the xGen Exome Panel V2 (Integrated DNA Technology ...
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bioRxiv - Genetics 2024Quote: ... One microliter of each primer pool was combined with 50 ng of sample plasmid DNA and 25 µL Kapa Hifi Hotstart ReadyMix (Kapa Biosystems Cat# KK2602), and topped off to a total volume of 50 µL with water ...
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bioRxiv - Neuroscience 2024Quote: ... 40 ng per sample at 10 ng/µl for cortical samples were used to perform qPCR using Light Cycler 480 Probes Master mix (Roche Diagnostics, Mannheim, Germany) on Light Cycler 480 (Roche Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... 12 ng of genomic DNA was amplified in a 25 µL reaction comprised of 2x Kapa HiFi Master Mix (Roche cat # KK2601/2), 1 µL of 10 µM V4 515F/806R primers with Illumina adapters (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTGYCAGCMGCCGCGGTAA -3’ ...
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bioRxiv - Cell Biology 2020Quote: ... 140 mM NaCl) with 1% Triton X-100 and cOmplete Protease Inhibitor Cocktail (Roche). After centrifugation at 17,000 g ...
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bioRxiv - Neuroscience 2021Quote: ... 1% Triton-X-100) supplemented with 1x protease inhibitor (Complete mini EDTA-free, Roche, Switzerland and 1x phosphatase inhibitor (PhosStop ...
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bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 µL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 µL nuclease free water ...
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bioRxiv - Biochemistry 2019Quote: ... 0.2% Triton X-100 and 1 cOmplete EDTA-free protease inhibitor cocktail tablet (Roche) per 50 mL and rotated slowly for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed using 100 μL RAPA lysis buffer containing protease inhibitors cocktail (Roche; Penzberg, Germany), and separated by a 10% SDS-PAGE ...
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bioRxiv - Genomics 2019Quote: ... 0.2% Triton X-100) supplemented with 1 X EDTA-free protease inhibitor cocktails (Roche) and 1 mM PMSF (AMRESCO) ...
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bioRxiv - Cell Biology 2019Quote: ... 0.5% deoxycholate and 1% Triton X-100) containing protease and phosphatase inhibitors (Roche, Switzerland). Before loading onto 4– 12% NuPAGE Bis-Tris gradient gels (Life Technologies) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested using Endoproteinase Lys-C at 1:100 w/w (Roche, 11058533103) at 37 °C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... and 1% Triton X-100) supplemented with 1xEDTA-free complete protease inhibitor mixture (Roche) and phosphatase inhibitor cocktails-II ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2% Triton X-100 and 1X protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche) and gently mixed at 4°C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... Antibody was removed by the addition of 100 µl homogeneous Protein G-Agarose (Roche) and a further incubation for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... and 100 mM potassium iodide) with EDTA-free proteinase inhibitor mixture (Roche Molecular Biochemicals). Lysates were cleared by centrifugation at 10000 rpm for 10 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% Triton X-100) in the presence of protease/inhibitor (Roche Applied Science) and phosphatase inhibitors (Calbiochem) ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100) supplemented with protease and phosphatase inhibitor cocktails (Roche, Sigma-Aldrich). Proteins were resolved by SDS-polyacrylamide gel electrophoresis (12% and 7% polyacrylamide gels for viral E and M proteins ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2% Triton X-100 and 1 cOmplete EDTA-free protease inhibitor cocktail tablet (Roche) per 50 mL and rotated slowly for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 mM NaCl) supplemented with cOmplete™ ULTRA Protease Inhibitor Cocktail tablets (Roche 5892970001) and PhosSTOP™ phosphatase inhibitor tablets (Roche 4906845001) ...
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bioRxiv - Molecular Biology 2020Quote: ... or Anti-Fluorescein-POD antibody (1:100, Anti-Fluorescein-POD, Fab fragments, 11426346910, Roche) was added and the slides were incubated at 4 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-DIG-POD antibody (1:100 dilute, Anti-Digoxigenin-POD, Fab fragment, 11207733910, Roche) or Anti-Fluorescein-POD antibody (1:100 ...
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bioRxiv - Molecular Biology 2019Quote: ... and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche; 1:100 dilution). Samples were mounted using SlowFade Diamond Antifade with DAPI mounting media (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM EGTA and 0.2% Triton X-100) containing the phosphatase inhibitor PhosSTOP (Roche) and complete protease inhibitor cocktail (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl complete LB3 (LB3 containing 1x Roche cOmplete Mini ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies were used at the following concentrations: mouse anti-GFP monoclonal (1:100; Roche), rabbit anti-Pav polyclonal (1:250 ...
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bioRxiv - Cell Biology 2019Quote: ... 1% Triton X-100) with 50 mM imidazole and Complete protease inhibitor tablets (Roche). Lysates were centrifuged at 10,000 g for 30 min and the supernatants were coupled to Fastflow nickel-sepharose (GE ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates (100–200 μg) were then immunoprecipitated with GFP antibody (Roche Applied Science). Ubiquitination was detected as described previously (38).
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bioRxiv - Biochemistry 2021Quote: ... 1% Triton X-100) supplemented with 1X Complete Protease Inhibitor Cocktail (Roche, Sigma-Alrdrich) at 4°C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... and 100 mM potassium iodide) with EDTA-free proteinase inhibitor mixture (Roche Molecular Biochemicals). Lysates were cleared by centrifugation at 10000 rpm for 10 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... eWAT digestion buffer was prepared as follows: 100 mg collagenase D (Roche, Cat#11088882001) was dissolved in a solution of 10 mL PBS ...
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bioRxiv - Microbiology 2020Quote: ... the column was treated with recombinant DNase I (20 units/100 μL; Roche Diagnostics) for 30 min at 37°C and RNA was eluted in 50 μL nuclease free water ...
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bioRxiv - Immunology 2020Quote: ... 2mM EDTA) with 100 mM PMSF and phosSTOP and a cocktail of antiproteases (Roche). A total of 40 μg proteins was separated by SDS-PAGE and analyzed by western blot ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% Triton X-100 with cOmplete protease inhibitor cocktail and PhosSTOP tablets (Roche). Cells were scraped and lysates clarified by centrifugation at 13,000 rpm at 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 1% Triton-X-100 supplemented with 10% glycerol and complete protease inhibitor cocktail (Roche). Lysates were cleared by centrifugation at 21,000xg for 20 min at 4 ⁰C ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% Triton X-100) with cOmplete protease inhibitor cocktail and PhosSTOP tablets (Roche) was added for 15 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... And 100 μM MG132 (Selleck) and 1 x EDTA-free protease inhibitor cocktail (Roche) were freshly added in the extraction buffer ...
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bioRxiv - Microbiology 2023Quote: ... 1% Triton X-100 and 0.1% DOC 10%) containing complete protease inhibitor cocktail (Roche). Lysates were cleared by centrifugation for 15 min at 13,000 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% [v:v] Triton X-100) supplemented with cOmplete EDTA-free protease inhibitor cocktail (Roche). Samples were subjected to sonication on ice (10 cycles consisting of 10-s impulses at an output 10% followed by 20-s intervals ...
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bioRxiv - Physiology 2024Quote: ... and 1 Na3VO4 and 1% Triton X-100 with PhosSTOP inhibitor tablet (Roche; 4906845001) and protease inhibitor cocktail (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... MDMs were also treated with 100 µM of T20 HIV-1 fusion inhibitor (Roche) or 3 µg/ml EFZ ...
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bioRxiv - Biochemistry 2023Quote: ... 0,3% Triton X-100) supplemented with cOmplete™ EDTA-free Protease Inhibitor (Roche, #05056489001) and 20 mM NEM ...
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bioRxiv - Immunology 2023Quote: ... 1 % Triton X-100 and 0.5 % deoxycholate) supplemented with EDTA-free protease inhibitor (Roche) and 0.1 μM PMSF ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were supplemented with 0.5% Triton X-100 and cOmpleteTM Protease Inhibitor Cocktail (Roche), incubated on ice for another 15 min and spun down at 1000 g for 5 min at 4°C ...