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Citations for Roche :
2251 - 2300 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 1% Triton X-100) supplemented with a protease inhibitor cocktail (Roche, Basel, Switzerland) on ice for 20 min with occasional agitation ...
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bioRxiv - Neuroscience 2024Quote: ... Each resulting denatured protein sample was chemically reduced with 100 mM dithiothreitol (Roche) at 56°C for 30 min and alkylated using 100 mM iodoacetamide for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Triton X-100) containing a protease inhibitor cocktail (#11836145001, Roche, Basel, Switzerland) and phosphatase inhibitors (04906837001 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 µL of cell proliferation reagent WST-1 (Roche, #11 644 807 001) was added to each well ...
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bioRxiv - Microbiology 2024Quote: ... prior to the addition of 100 μL BM Blue POD soluble Substrate (Roche). Reaction was stopped with 1 N HCl ...
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bioRxiv - Neuroscience 2024Quote: ... cells were then exposed to blocking buffer containing 0.2% Triton X-100 (Roche) in 50% normal goat serum (NGS ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 % w/v Triton X-100 supplemented with protease inhibitor tablets (Roche), 1 mM sodium fluoride and 1 mM sodium vanadate) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 % w/v Triton X-100 supplemented with protease inhibitor tablets (Roche), 1 mM sodium fluoride ...
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bioRxiv - Cell Biology 2024Quote: ... 12.5 mM EDTA) containing 100 μl proteinase K (recombinant PCR grade solution, Roche) was added to each sample and digested at 65 °C for 15 min ...
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bioRxiv - Immunology 2024Quote: ... 1% Triton X-100) supplemented with cOmplete™ Protease Inhibitor Cocktail (#11836170, Roche). Lysates were then cleared by centrifugation for 15 minutes at 14,000 rpm at 4°C and subjected to immunoprecipitation with anti-V5 mouse monoclonal antibody (#MCA1360 ...
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bioRxiv - Cancer Biology 2021Quote: ... size and concentration of cDNA libraries were checked using the Standard Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Hiseq 2500 sequencer as single-end 125 base reads ...
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed in duplicates with 20 ng template cDNA per reaction using KAPA SYBR® FAST (KK4603, Roche, Basel, Switzerland) and a CFX96™ Real Time System C1000 Touch™ Thermal Cycler (BioRad ...
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bioRxiv - Genomics 2019Quote: ... using the HS NGS Fragment kit (DNF-474-33) and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). Each library was sequenced on half a lane of a rapid v2 flow cell (Illumina ...
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bioRxiv - Genomics 2019Quote: ... using the HS NGS Fragment kit (DNF-474-33) and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). The library was sequenced on one lane of a rapid v2 flow cell (Illumina ...
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bioRxiv - Genomics 2019Quote: ... Adapters-ligated cDNAs were then amplified by PCR and libraries were validated on Fragment Analyzer with a Standard Sensitivity NGS kit (Advanced Analytical Technologies, Inc) and quantified by qPCR with a Light Cycler 480 thermal cycler (Roche Molecular diagnostics).
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bioRxiv - Genomics 2019Quote: ... trachomatis molecular diagnosis was performed by using a dual-target (chromosome plus plasmid) commercial nucleic acid amplification test (NAAT) (Cobas® 4800 CT/NG from Roche Diagnostics) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The gel piece was vacuum dried and treated with trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche sequencing grade) at 37°C for 16 hours ...
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bioRxiv - Microbiology 2020Quote: ... ChIPs were performed by combining sonicated chromatin (500 ng DNA content) with either 1 μg mouse mAb α-GFP (Roche Diagnostics #11814460001) or 1 μg rabbit α-PfHP1 12 in incubation buffer [(5% Triton-X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was diluted to a final concentration of 5 ng/μl and RT–qPCR was performed with KAPA SYBR® FAST qPCR kits (KAPA Biosystems) on a C1000 thermal cycler ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries were prepared starting from 500 ng for each DNA sample with the KAPA HyperPrep Kit (Hoffman-La Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: RNA template (500 ng) was used for one-step RT-PCR amplification protocol (SYBR Green I RNA amplification kit, Roche, Basel, Switzerland). Primers were designed using the LC Primer/Probe design software (Roche ...
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bioRxiv - Physiology 2024Quote: ... 20 ng cDNA was used for real-time qPCR reactions with KAPA SYBR FAST qPCR Kit (Catalog no. KK4618, Roche, Basel, Switzerland) using a CFX Opus Real-Time PCR System (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... size and concentration of cDNA libraries were checked using the High Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Novaseq 6000 sequencer as single-end 100 base reads ...
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bioRxiv - Cancer Biology 2023Quote: ... Strand-specific and barcode-indexed RNA-Seq libraries were generated from 300 ng total RNA each after poly-A enrichment using the mRNA-Seq Hyper Kit (Kapa Biosystems, KK8581) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... this PCR was performed in eight reactions of 25 μl (200 μl total) using 150 ng of 4C-template per reaction and the Expand Long Template PCR System (Roche, Basel, Switzerland) (Supplementary Tables 3 and 4) ...
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bioRxiv - Physiology 2023Quote: ... libraries were verified on a Fragment Analyzer using the High Sensitivity NGS kit (#DNF-474-0500) and quantified using the KAPA Library quantification kit (Roche, ref. KK4824).
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bioRxiv - Immunology 2023Quote: ... The amount of RNA was adjusted to a minimum of 600 ng in each sample and cDNA synthesis was performed with EvoScript Universal cDNA Master (Roche, Basel, Switzerland) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... Then DNA fragments underwent a NGS library preparation procedure consisting in end repair and Illumina adaptor ligation using the KAPA HTP kit (Roche, Basel, Switzerland). Individual index sequences were added to each library for identifying reads and sorting them according to their initial origins ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized by a reverse transcriptase reaction with 500 ng of total RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basal, Switzerland), and was used as a template for qPCR with LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Average library size and quality control were performed using a Fragment Analyzer (High Sensitivity NGS kit) and qPCR (Roche Light Cycler 480). CUT&RUN libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA stranded mRNA kit (v4.16) (Kapa Biosystems, Wilmington, MA). RNA was sheared to 300-400 bp ...
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bioRxiv - Cancer Biology 2023Quote: 15 ng of each patient’s cDNA library were amplified using the KAPA HiFi HotStart Uracil+ ReadyMix 2x (Kapa Biosystems, cat. no. KK2801) with 0.5 µM final concentration of custom-primers (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... brought to a final volume of 10 ml with 10% polyvinylalcohol solution) supplemented with 4.5 µl/ml NBT (Roche) and 3.5 µl/ml BCIP (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysis buffer was supplemented either with 40 U/ml RNase inhibitor (Molox) or 50 U/ml Benzonase (Roche) to differentiate between RNA- and protein-dependent interactions ...
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bioRxiv - Immunology 2022Quote: ... tissues were minced and placed into gentleMACS C tubes (Miltenyi 130-093-237) containing 15 mL enzyme digestion solution (10 mM HEPES, 0.2 U/mL Liberase TM [Roche 5401127001] ...
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bioRxiv - Cancer Biology 2020Quote: ... and pancreatic tumor bearing mice by mechanical separation of the tumor followed by digestion with an enzymatic cocktail consisting of 1mg/ml collagenase A and collagenase D and 0.4mg/ml DNase I (all from Roche) in PBS at 37°C for 2-3h with rotation at 600rpm ...
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bioRxiv - Neuroscience 2019Quote: ... The brains were transferred to an 1.5 ml Eppendorf tube containing 500 microliters 1 mg/ml Liberase DH (Roche, prepared according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2019Quote: ... The chopped tissue was then put in a mixture of 1mg/ml collagenase D and 0.1mg/ml DNase I (Roche) in HBSS (Lonza) ...
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bioRxiv - Immunology 2021Quote: ... The pancreas was inflated via the common bile duct by adding ∼3 ml of 0.8mg/ml Collagenase P (Roche) and 10µg/ml Dnase I (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... then 10-15 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) containing 0.4 mg/ml Protease (Roche) and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml ...
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bioRxiv - Microbiology 2020Quote: ... pellets were re-suspended in 1.2 mL of Lysis Buffer (50 μg/ml lysozyme, 0.8% NP-40, 1 mM MgCl2, 1x protease inhibitors [Roche; cOmplete Protease Inhibitor Cocktail] in PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Peels were cut in 2-5 mm2 pieces and placed in 5 ml digestion solution (0.75 mg/ml Liberase TH Research grade (Roche), 0.1 mg/ml DNAseI (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were injected in multiple locations with a total of 1.5 mL RPMI containing 50 µg/mL Liberase DL research grade (Roche) and 10 U/mL DNase I (Sigma) ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... infused with digestion buffer (RPMI 1640 [Sigma], Liberase TL [0.1 mg/mL; Roche], and DNase [0.1 mg/mL; Roche]) and minced with scissors ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor tissues were manually minced and digested in 25 mL of Dulbecco’s Modified Eagle Medium (DMEM) containing 2 mg/mL of collagenase A (Roche) and DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were resuspended in 20 ml Tris- HCl (20 mM, pH7.5) supplemented with 10ug/mL DNase I (Roche, 10104159001) and ½ tablet cOmplete Protease Inhibitor Cocktail (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... DRGs were dissociated in 1 μg/ml collagenase A plus 2.4 U/ml dispase II (enzymes, Roche Applied Sciences) in HEPES-buffered saline (HBSS ...
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bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
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bioRxiv - Cell Biology 2020Quote: ... were incubating over night at 4°C with 0,375 mg/ml of NBT and 0,188 mg/ml BCIP dissolved in a solution of TMNbuffer (respectively #11383213001 and #11383221001, Roche).
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were injected in multiple sites with a total volume of 1.5 mL Liberase enzyme digestion solution (50 μg/mL Liberase DL research grade (Roche) and 10 U/mL DNase I (Sigma ...