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Citations for Roche :
2351 - 2400 of 7151 citations for Human Protein Patched Homolog 2 PTCH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PCR reactions with a total volume of 25 μL included: 2× KAPA HiFi Hot Start Ready Mix (KAPA Biosystems), a final concentration of 0.1 mM for each primer and 7.5 μL of DNA sample ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µl of cDNA were used for Real-Time PCR with self-designed primers and SYBR green reaction (Roche). qRT-PCR reactions were performed in duplicates including non-template controls on a QuantStudio 3 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... rat cortical neurons cultured as described above were lysed directly in buffer (50 mM HEPES pH 7.0, 2% SDS, 1 mM EDTA plus protease inhibitor mixture (PIC, Roche) and 20 mM MMTS (to block free thiols) ...
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bioRxiv - Biochemistry 2023Quote: ... Ndel1) were diluted in assay buffer at concentrations indicated in Figure legends supplemented with 2 mM AMP-PNP (Roche). Prior to image acquisition ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hr and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Immunology 2023Quote: ... lysed by ice-cold RIPA buffer (50 mM Tris pH8, 150 mM NaCl, 2 mM EDTA, 0.5% NP40) supplemented with protease inhibitors (Roche) and by sonication ...
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bioRxiv - Developmental Biology 2023Quote: ... The slides were washed and detection was performed at pH 9.5 by incubating in nitro blue tetrazolium and 5-bromo-4-cholro-2-indoyl phosphate solution (Roche) as per manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 2% (w/v) SDS and immediately prior to use supplemented with cOmplete™protease inhibitor cocktail (Roche. Cat# 04693116001) and PhosStop (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... The clarified lysate was then diluted 3.75 times using dilution buffer (10 mM Tris–HCl, pH 7.4, 2 mM EDTA, 1 mM DTT, protease inhibitor cocktail tablet (cOmplete, Roche) and phosphatase inhibitor (phosSTOP ...
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bioRxiv - Immunology 2023Quote: ... et al.86 LNs were incubated in 2 mL of a freshly prepared digestion enzyme mix containing 0.8 mg/ml Dispase II (Roche), 0.2 mg/ml Collagenase P (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... cells were lysed with TNE-lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and complete Protease Inhibitors, Roche) for 30 min on ice then spun down at 11,000 rpm for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA was synthesized from 2 μg of total RNA using Superscript II reverse transcriptase (Roche, Mannheim, Germany). qPCR was performed in a 20 μl reaction mixture containing 10 μl of SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hour and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... in complementary deoxyribonucleic acid (cDNA) from 10 ng total RNA in 10 μl reactions with 2× Master Mix (Roche) in a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
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bioRxiv - Molecular Biology 2023Quote: SDGC-SEC-purified stress granule cores (strain JD1370) were incubated at 0.23 A260 units/mL with 2 or 4 units of RNase H (10786357001; Roche) in 40 μL of RNase H buffer (20 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... RPE explants were collected in 60 μl of lysis buffer (Tris-HCl 62.5 mM, SDS 2%, pH 6.8) with protease (Roche, #1183617001) and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Transgenes were identified using PCR primers specific for each transgene (Suppl. Table 2) amplified using Taq DNA polymerase (Roche) and Failsafe Buffer D (Epicentre) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in 4 times the pellet volume of sucrose buffer (10 mM HEPES, pH 7.5, 250 mM sucrose, 2 mM magnesium acetate, 1X cOmplete EDTA-free protease inhibitor cocktail [Roche]) and lysed by douncing at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and resuspended in solubilization buffer (50 mM HEPES/KOH pH 7.5, 200 mM NaCl, 2 mM MgAc2, 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche] ...
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bioRxiv - Biophysics 2023Quote: Cell lysis was performed by resuspending cell pellets in lysis buffer (50 mM Tris-HCl, 250 mM NaCl, 0.5 mM DTT; 2 µM avidin, protease inhibitors cocktail (Roche) and DNAse (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were harvested by centrifugation and resuspended in 20 mL of lysis buffer (50 mM Tris pH 7.5, 300 mM NaCl, 2 mM MgCl2 and 1 mM DTT, 1X Roche EDTA-free Complete protease inhibitor cocktail) ...
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bioRxiv - Immunology 2024Quote: Five female mosquitoes were collected in 250 μL of 2× RIPA buffer complemented with protease inhibitor (Complete 1×, Roche) in tubes containing ∼20 1-mm glass beads (BioSpec) ...
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bioRxiv - Developmental Biology 2024Quote: ... whole-exome DNA from blood or salivary samples was captured using the Nimblegen v.2 exome capture reagent (Roche) or Nimblegen SeqxCap EZ MedExome Target Enrichment Kit (Roche) ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated in 50% Hybridization Buffer (50% formamide, 2x SSC, 1 mg/ml Torula RNA, 0.05 mg/ml Heparin, 2% Roche blocking reagent ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were analyzed for glucose production rate at 2 h by using an Accu-Chek® Aviva glucometer (Roche Diagnostics Scandinavia AB ...
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bioRxiv - Biophysics 2024Quote: ... 2 million cells were seeded in a 10-cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Broncholalveolar lavage (BAL) fluids were obtained by intratracheal injection of 2 × 1 ml PBS supplemented with protease inhibitors (Roche). Serum was collected by intracardiac puncture and separated from whole blood in Z-Gel microtubes (Sarstedt) ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... embryos were with PBT (x3, 15mins) and incubated in prehybridization buffer (50% deionized formamide, 5× SSC, pH 4.5, 2% Roche Blocking Reagent ...
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bioRxiv - Developmental Biology 2024Quote: ... 100 μl of MNase buffer (0.3 M Sucrose, 85 mM Tris, 3 mM MgCl2, 2 mM CaCl2, 2.5U of micrococcal nuclease: Roche 10107921001) was added in to each tube (0.5 millions of cells per tube) ...
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bioRxiv - Molecular Biology 2024Quote: ... SDS (0.2%), Triton X-100 (2%), sodium deoxycholate (1%), Tris HCl (100 mM, pH 8.0)) with protease inhibitor (Roche #11836145001), then incubated on ice for 30 m ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed in 200 μL lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF, cOmplete Protease Inhibitor Cocktail, Roche), heated to 99 °C for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... pooled and quantified (KAPA library quantification kit from KAPA Biosystems), and sequenced with the MiSeq system (Illumina).
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bioRxiv - Developmental Biology 2021Quote: ... and by qPCR using a KAPA Library Quantification Kit (Roche) for Illumina platforms ...
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bioRxiv - Cell Biology 2020Quote: ... Revelation was performed using the Ultraview DAB revelation kit (Roche). Nuclei were counterstained with hematoxylin solution (Dako) ...
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bioRxiv - Developmental Biology 2021Quote: ... quantified with a Kapa Library Quantification Kit (Kapa Biosystems KK4824), and sequenced on an HiSeq 2500 platform (Illumina ...
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bioRxiv - Genetics 2021Quote: ... and libraries were prepared using KAPA HyperPrep Kit (Roche, 07962347001) using KAPA DUI adapters (Roche ...
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bioRxiv - Microbiology 2021Quote: ... and the MagNA Pure Compact DNA Isolation Kit I (Roche) following the protocol “Total_NA_Plasma_external_lysis purification protocol” ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using Roche High Pure™ PCR Template Preparation Kits (Roche Molecular Systems ...
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bioRxiv - Developmental Biology 2020Quote: ... TUNEL labeling (In Situ cell death detection kit, Fluorescein, Roche) was performed according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Libraries were prepared using the KAPA mRNA HyperPrep kit (Roche) and sequenced as 76bp single-end ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were quantified by KAPA library quantification kit (KAPA biosystems) and then multiplexed and sequenced on Illumina Hiseq 4000 platform to obtain 150 nt paired-end reads.
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bioRxiv - Bioengineering 2022Quote: ... RNA was extracted using High-Pure RNA Isolation Kits (Roche) and assessed for concentration and purity using the NanoDrop 2000c – UV- Vis spectrophotometer ...
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bioRxiv - Developmental Biology 2022Quote: ... using the In Situ Cell death detection Kit (Roche, 12156792910). All samples were counterstained in 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Genomics 2020Quote: ... using Kapa HyperPlus KK8514 library kit (Kapa Biosystems, Wilmington, MA). DNA was enzymatically sheared to approximately 600bp fragments ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed using KAPA2G Robust PCR kits (KAPA Biosystems). A typical 25 μl reaction used 20–100 ng of genomic DNA ...
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bioRxiv - Developmental Biology 2019Quote: ... and detected with the DIG Nucleic Acid Detection Kit (Roche). Wing imaginal discs were mounted in glycerol and imaged with a Nikon E200 bright-field microscope.
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bioRxiv - Microbiology 2020Quote: ... the KAPA Stranded mRNA-Seq library preparation kit (Kapa Biosystems) was used to prepare library samples and sequenced on the NextSeq platform (Illumina ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or the KAPA Library Quantification Kit for Illumina (KAPA Biosystems). Sequencing was performed on Illumina instruments using the HiSeq SBS Kit v4 250 cycles kit (Illumina ...
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bioRxiv - Genomics 2019Quote: ... Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems) was used to quantify RNA libraries by qPCR ...