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Citations for Roche :
2201 - 2250 of 7151 citations for Human Protein Patched Homolog 2 PTCH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... The biotinylated protein was incubated with 50ug fresh mitochondria extraction in import buffer containing ATP regeneration system (Creatine kinase (Roche 10127566001), Creatine Phosphate (Sigma-Aldrich 10621714001 ...
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bioRxiv - Genetics 2022Quote: ... 10 μg total proteins were separated on a 12% SDS-PAGE gel and transferred to the PVDF membranes (Roche, CA, USA) using an electro-transfer apparatus (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant containing the immunoprecipitated protein was resolved in a 10% SDS-PAGE gel and detected by an anti-HA antibody (Roche, 12158167001).
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene difluoride membrane (Roche, Basel, Switzerland). Membranes were incubated (overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Hand dissected ovaries from 100-150 flies (depending on the expressed protein) of appropriate genotypes were lysed in RIPA buffer supplemented with Complete Protease Inhibitor cocktail (Roche, 11836170001) and 20 mM NEM ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg nuclear lysate was adjusted to 500 uL in Protein Incubation Buffer (PIB+++; 150mM NaCl, 50 mM Tris pH 8.0, cOmplete protease inhibitor (Roche, cat 04693159001), 0.25% NP-40 and 1 mM DTT) ...
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bioRxiv - Neuroscience 2024Quote: Weighed brain tissue samples were processed for protein assays by addition of 10X volume NP-40 buffer with added cOmplete Protease Inhibitor (Roche #04693132001) and PhosStop (Roche 04906837001 ...
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bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (normal CSF protein content lies in the 0.2-0.4 g/L range (Roche et al., 2008)) ...
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bioRxiv - Neuroscience 2023Quote: Proteins were detected by Western blot analysis after separating 30–50 μg of total protein lysate prepared in RIPA buffer with protease inhibitor cocktail (cOmplete™, Roche) on a 4– 15% Mini-PROTEAN® TGX™ Precast Gel (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein lysates were prepared in radioimmunoprecipitation assay buffer supplemented with a protease inhibitor mixture (Roche Applied Science, IN, USA) and 2mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... beads with protein were subjected to overnight digestion by adding 100 µl 5 ng/µl sequencing grade trypsin (Roche Diagnostic GmbH) in ammonium bicarbonate (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: Whole-worm protein extracts were generated by sonication in radioimmunoprecipitation assay (RIPA) lysis buffer with cOmpleteTM Protease Inhibitor Cocktail (Roche #4693116001) and β-glycerophosphate (Sigma-Aldrich G6251) ...
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bioRxiv - Bioengineering 2023Quote: ... lung tissues were thawed on ice and then resuspended in 0.5ml of Tissue Protein Extraction Reagent (T-PER) (ThermoScientific, cat# 78510) containing Complete Mini Protease Inhibitor Cocktail (Roche, cat# 11836170001). Tissues were homogenized using an electric handheld tissue homogenizer and were stored at -80°C until used for ELISA.
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bioRxiv - Molecular Biology 2023Quote: ... containing either single (for the IgG screen) or serial dilutions (for full IgG multi-point kinetics) of HEL protein (Roche #10837059001). For measurement of Fab binding kinetics ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.4 with 0.1% Triton X-100) and then incubated with 350 µg protein in 1:1 8M urea with Complete protease inhibitor (Roche 11245200) sample lysis:Buffer A/0.5% Triton X-100 (150 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial lysis and protein digestion were performed simultaneously by adding 50 µL of 1 mg/mL trypsin (Roche Diagnostics, Mannheim, Germany) prepared in a 150 mM NH4HCO3 solution ...
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bioRxiv - Biophysics 2024Quote: ... Cultures were grown until an A600 of 0.6-0.8 was reached and protein over-expression was induced by incubating with 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG, Roche) for 16 hours at 18°C.
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bioRxiv - Biophysics 2024Quote: ... AF488-GAF-FL or AF546-GAF-ΔPOZ was flown through the protein channel diluted to 80 nM in imaging buffer (50 mM NaCl, 50g/mL BSA (Roche 10711454001), 0.05% NP40 (Sigma I8896) ...
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bioRxiv - Microbiology 2023Quote: ... The pooled library was cleaned using MO BIO UltraClean® PCR Clean-Up Kit (MO BIO, Carlsbad, CA) and quantified using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Sequencing was carried out as detailed in the EMP protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... mixed-stage transgenic worms expressing CDE-1::GFP were resuspended in equal volumes of 2× lysis buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 1% Triton X-100, Roche®cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2020Quote: ... 50 mM Tris/HCl pH 7.4/50 mM NaF, 10 mM Na4P2O7, 2 mM MgCl2 and Complete Mini, EDTA-free protease inhibitor [Roche]). Lysates were cleared and incubated with GFP-Trap agarose beads for 60 min ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in Immunoprecipitation buffer (150 mM NaCl, 10 mM Hepes, 2 mM EDTA, 1% Triton, 1.5 mM MgCl2, 10% glycerol, protease inhibitor from Roche) and centrifuged at 14,000 × g ...
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bioRxiv - Cell Biology 2019Quote: ... mechanically disintegrated and partially digested in a solution of collagenase and trypsin [2 mg/ml collagenase (Roche or Sigma), 2 mg/ml trypsin (Dutcher Dominique or Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 2% SDS and 50 mM DTT with the addition of anti-protease (cOmplete cocktail, Roche 11 873 588 001). Samples were boiled 5 min at 95°C before loading on polyacrylamide gels ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal (3F10) antibody directed against the HA epitope and monoclonal (B-2) antibody against GFP were purchased from Roche and Santa Cruz Biotechnology (Dallas ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 mM EDTA, 15% glycerol, 2 mM MgCl2, 1 mM DTT, 1 mM PMSF, and protease inhibitor cocktail [Roche]). Following clarification by centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-μl aliquots of all cDNA samples were analyzed in triplicate on 96-well optical PCR plates (Roche Diagnostics). GAPDH or TBP was used as the reference gene and all analyses were performed using the ΔΔCt method with Roche LightCycler 96 system software ...
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bioRxiv - Developmental Biology 2021Quote: ... apob-1 and apob-2 levels were detected using the Fast Start Essential Green DNA master mix (Roche 06924204001) on a Roche LightCycler 96 instrument ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked in 1xBM Blocking/PBST at RT for 2 hours and incubated with anti-DIG-POD (1:1,000, 11207733910, Roche) at 4°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... DRGs from E13.5 embryos were dissected and placed in droplets of 2 mg/ml collagen (Roche Diagnostic, catalog#11179179001) together with COS1 aggregates transfected with either secreted Myc-Sema3A or control PAY1-GFP ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA probes were incubated for 2 hours at 37 degrees with Dig RNA labeling mix (11277073910, Roche, Basel, Switzerland) and SP6 or T7 RNA polymerase (RPOLSP6-RO and RPOLT7-RO ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl of PCR gene specific primer (10X conc) (Table S1) and 10 μl of master mix (Roche 04707516001) in 20 μl reaction ...
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bioRxiv - Genomics 2020Quote: ... the pellets were washed with ice-cold nuclei suspension buffer (1X PBS containing 2% BSA and 0.2 U/µl Protector RNase Inhibitor, ROCHE) and filtered through a 30μm cell strainer (Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... For this 1 ml of worm pellet was mixed with 1 ml of 2x lysis buffer (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 2% Triton, 0.5 tablet of protease inhibitor (Roche, 05892791001)) ...
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bioRxiv - Cancer Biology 2019Quote: ... The PCR reaction (20 μL) consisted of 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48 h and resuspended in lysis buffer (50 mM HEPES pH 7.2, 200 mM NaCl, 10% glycerol, 2 mM DTT, and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed using a microfluidizer ...
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bioRxiv - Developmental Biology 2019Quote: ... Trunks from ∼60 embryos were homogenised in 1ml solubilisation medium [2%w/v CHAPS in PBS made 1mM with sodium orthovanadate and 1 tablet cØmplete protease inhibitor cocktail (Roche) per 50ml of solution] on wet ice by shearing through a 20G then 26G needle ...
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bioRxiv - Genomics 2020Quote: ... Cell suspension was then supplemented with 1 mM Tris (2-carboxyethyl) phosphine (TCEP) and 1× complete inhibitor cocktail (Roche). Cells were lysed by sonication on ice and then centrifuged at 80,000 g at 4 °C for 30 min ...
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bioRxiv - Immunology 2019Quote: ... diluted 1:150 in washing buffer (45 min) and 4’,6-diamidino-2-phenylindole counterstain (DAPI; Roche/Sigma-Aldrich) diluted 1:250 in TBS (15 min ...
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bioRxiv - Cancer Biology 2019Quote: ... coli cells containing the overexpressed RNF114 fusion protein were re-suspended in 80 mL of lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 2 Roche protease inhibitor tablets [without EDTA] ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol, Roche Complete Protease Inhibitors and PhosSTOP Protein Phosphatase Inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) and protease inhibitor cocktail (Roche) on ice for 20 min ...
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bioRxiv - Genetics 2019Quote: ... Next embryos were washed in blocking solution (100mM maleic acid, 150 mM NaCl pH 7.5, 2% blocking reagent (Roche)) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Lungs were dissected and digested for 30min at 37°C using RPMI media (2% FBS+ Pen/Strep, glutamine, 10mM HEPES) containing 0.5mg/mL Collagenase D (Roche). The digested fragments were passed through a 70μm cell strainer (Greiner bio-one ...
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bioRxiv - Molecular Biology 2020Quote: ... and subjected to 8 cycles of lysis in a MagNALyser (Roche – 6000 rpm, 1 min on, 2 min off). Lysate was transferred to fresh microfuge tubes and clarified by centrifugation (13,000 rpm ...
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bioRxiv - Cancer Biology 2021Quote: Human tumor samples were collected on different days right after surgery and digested in Hank’s Balanced Salt Solution supplemented with 2 mg/mL collagenase A (Roche), 2.5 U/mL hyaluronidase (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ATP and 10% glycerol) containing 1 mmol/L PMSF and DNase I (Roche Diagnostics Corp., IN, USA) to 5 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in ice-cold homogenizing buffer (20 mM HEPES pH 7.4, 250 mM sucrose, 2 mM MgCl2, and EDTA-free protease inhibitor cocktail [Roche]), and homogenized on ice by 25 passages through a 26-guage needle fitted to a 1 mL syringe ...