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Citations for Roche :
2351 - 2400 of 8584 citations for 6 AMINO 4H 7H 1 3 DITHIINO 5 4 D PYRIMIDIN 8 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized by adding 4 units of AMV reverse transcriptase enzyme (Roche) and 0.5 μl 20 pmol/μl of reverse primer labeled with [32P] per reaction ...
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bioRxiv - Cell Biology 2020Quote: ... 1ml of digestion solution (TM Liberase at 4 units/mL (Roche, Basel, Switzerland) and DNAse Type I at 800 units/mL (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... qPCR mastermixes were prepared with 4 μl Light Cycler Enzyme Mix (Roche, Germany), 0.6 μl 10 μM primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 200 μl/ml of a 4% stock solution of blocking reagent (Roche 11096176001) which was dissolved and autoclaved in MNT solution (150 mM maleic acid pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... AP reaction was developed with 4-Nitro blue tetrazolium chloride (NBT, Roche, 11383213001) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP ...
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bioRxiv - Immunology 2023Quote: ... Macrophages were polarized with 20 ng/mL recombinant IL-4 (Peprotech or Roche) or 100 ng/mL LPS (E ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were incubated for 4 h with 10 ng/ml colcemid (Roche, 10295892001). Cells were then collected and incubated for 15 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 mM EDTA) supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail (Roche). Proteins were resolved on a 4%–12% bis-tris gel (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 4 ng of ChIP DNA with KAPA Library Preparation Kit (KAPA Biosystems) and NimbleGen SeqCap Adaptor Kit A or B (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 mM Sodium Orthovanadate and supplemented with protease inhibitors (Complete protease inhibitors, Roche). After a 20min incubation on ice ...
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bioRxiv - Genetics 2020Quote: ... The final linked-read library (8 cycles of PCR amplification) was quantified using the Kapa Library Quantification Kit (Cat. No. KK4824) from Kapa Biosystems (Woburn, MA, USA) and diluted to 4 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... Frozen cell pellets were re-suspended in lysis buffer (50 mM Tris-HCl, 500 mM NaCl, 10% glycerol, pH 8) containing protease inhibitors (Roche, complete mini, EDTA-free). Following lysis by sonication and centrifugation at 20,000 r.p.m ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were pelleted at 800g for 10 min at 4°C and washed twice in buffer 1 and once in freezing buffer (1M Tris pH 8, 100% glycerol, 100mM MgAc2, 0.5M EDTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion). Nuclei were resuspended in freezing buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 8 μCi of α-32P-[ATP] and α-32P-[UTP] and 20 U of T7 RNA Polymerase or SP6 RNA Polymerase (Roche Applied Sicences). The reaction was incubated for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: Cells were pelleted and lysed with 1.5% n-dodecyl-D-maltoside (DDM) in PBS with cOmplete™ protease inhibitor (Roche) for 15 min on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... Dissected and pulverized TA samples were placed in TRIzol in Lysing Matrix D Tubes (MP Biomedical) and were homogenized in a MagNa Lyser machine (Roche) programmed to 7,000 RPM for a total of three 20-second bursts separated by 10-second cool-downs on ice ...
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bioRxiv - Physiology 2019Quote: ... Serum Vitamin D (S-25-OH-D) levels were measured in samples collected before and after the intervention using a electrochemiluminescence immunoassay (Roche Cobas Vitamin D total assay ...
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bioRxiv - Immunology 2022Quote: ... The tonsil and lung were gentleMACS dissociated in 5mL digestion buffer (RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche)) and then agitated on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fat pads were rinsed in PBS and minced in ice-cold digestion buffer (7.5 mg/ml Collagenase D (Roche 1108886001), and 4.8 mg/ml Dispase II (Sigma D4693 ...
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bioRxiv - Cancer Biology 2022Quote: ... genomic (g)DNA was extracted both from primary Irf4−/− tumours as well as FACS-sorted control B220+mIgM- BM fr.A-D cells using the High Pure PCR Template Preparation kit from Roche (11796828001). Integrity of resultant gDNA was confirmed in a 2 % Agarose gel ...
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bioRxiv - Immunology 2022Quote: ... were placed in 5mL of digest media (RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche)) and homogenized in gentleMACS C tubes (Miltenyi Cat#130096334 ...
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bioRxiv - Immunology 2020Quote: ... mice were perfused with sterile PBS and the right inferior lung lobes were digested at 37°C with 630 µg/ml collagenase D (Roche) and 75 U/ml DNase I (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... cellular pellets were suspended in 10 mL of Buffer 1X supplemented with 2 mM (L+D) 1,4-Dithiothreitol (DTT) and cOmplete™ mini protease inhibitor cocktail (Roche). To avoid overheating and protein denaturation ...
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bioRxiv - Immunology 2021Quote: ... the dura mater was carefully removed from the brain and then chopped for enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 15 mg/mL collagenase D (Roche) and 1 mg/mL DNase (Roche ...
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bioRxiv - Physiology 2020Quote: 1 μg RNA was reverse transcribed into cDNA with Oligo(d)T primers using the Transcriptor First Strand cDNA Synthesis kit (Roche). The qRT-PCR was performed with the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Immunology 2020Quote: ... Spleens were digested for 30 min at 37°C in digestion buffer [(RPMI 1640 supplemented 0.5 mg/ml collagenase D (Roche; 11088866001) and 10 µg/ml DNase I (Roche ...
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bioRxiv - Immunology 2022Quote: Excised tumors were cut into small pieces following by incubation 0.5 mg/ml collagenase D (Hoffmann-La Roche, Basel, Switzerland), 10 µg/ml DNAse I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: Spleen from C57BL/6J mice were mechanically disrupted using the back-end of a syringe before addition of 50 μL 11x concentrated collagenase D (Roche, #11088866001 ...
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bioRxiv - Immunology 2023Quote: Lungs were perfused with sterile PBS and the left lobes of the lungs were digested at 37°C with 630 µg/ml collagenase D (Roche) and 75 U/ml DNase I (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... in a plate and mechanically disrupted using the back-end of a syringe before addition of 50 μL of a digestion media (dRPMI = naRPMI supplemented with 11x collagenase D (#11088866001, Roche, Woerden ...
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bioRxiv - Immunology 2023Quote: ... Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche) and 0.1 mg/mL DNAse I ...
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bioRxiv - Microbiology 2023Quote: ... containing 5 mL dissociation buffer (RPMI-1640 (Cytiva) with 25 mM HEPES and 10 µg/mL gentamicin sulfate) supplemented with 2 mg/mL Collagenase-D (Roche) and 100 U DNaseI (Roche) ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was carried out in 12 μL volumes containing 6 μL of High Resolution Melting Master (Roche Applied Science, Germany), 0.24 μL of each 10 μM primer ...
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bioRxiv - Cell Biology 2020Quote: C2 myoblasts were transfected 18-24 h after plating at 70-80% confluence by using FuGENE 6 (Roche Diagnostics, Indianapolis, IN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Resulting cell pellets were resuspended in Buffer A (100 mM NaH2PO4, 10 mM Tris, 6 M GuHCl, 10 mM imidazole) + PIC (Roche 05056489001). Cells were sonicated 3X at 25% power for 10s (Cole-Parmer GE 130PB-1) ...
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bioRxiv - Molecular Biology 2020Quote: ... were added and after an additional 6 hours BrdU was added and a cell proliferation assay was performed according to the manufacturer’s instructions (Roche Applied Sciences).
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bioRxiv - Cell Biology 2021Quote: ... cells transfected with a combination of RCAS plasmids (RCAS PDGFB-HA, RCAS shp53-RFP) using the FuGENE 6 transfection kit (Roche, 11814443001) according to the manufacturer’s protocol and as previously described 86 ...
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bioRxiv - Microbiology 2022Quote: Mice were fasted for 6 h and baseline blood glucose levels were measured with an Accu-Check Performa blood glucose meter (Roche, USA) using blood collected from the tail vein ...
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bioRxiv - Immunology 2019Quote: ... Each reaction consisted of a total amount of 12 µL divided into 6 µL LightCycler 480 SYBR Green I Master (Roche Diagnostics), 2 µL primers ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragments were separated on 0.7% agarose gels buffered with 40 mM Tris-acetate at 4V/cm for 6 h and transferred overnight to nylon membranes (Roche, Switzerland) by alkaline transfer ...
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bioRxiv - Biochemistry 2023Quote: ... 0.6 mL of incubation buffer (citric acid 100 mM and NaCl 250 mM pH 5.5 with inhibitor cocktail (Roche, Rotkreuz, Switzerland) dilution according to the instructions of the manufacturer) ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of 2X KAPA HiFi HotStart Ready Mix (KAPA Biosystems, KK2602).
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bioRxiv - Biochemistry 2020Quote: ... 5% Glycerol and EDTA free protease inhibitor cocktail tablet/50ml (Roche, UK)] ...
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bioRxiv - Cell Biology 2021Quote: ... 5% glycerol ()AppliChem #A2926) and phosphatase inhibitors (PhosSTOP™, Roche, Indianapolis, IN), 2 mM sodium orthovanadate ...
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bioRxiv - Biochemistry 2021Quote: ... 5% (v/v) glycerol with complete EDTA-free protease inhibitor cocktail (Roche) and 10 µg/ml DNAseI (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM imidazole) supplemented with complete EDTA-free protease inhibitor tablets (Roche) and disrupted by sonication ...
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bioRxiv - Biochemistry 2021Quote: ... 5 %(v/v) glycerol and cOmplete EDTA-free protease inhibitor tablets (Roche).
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol with complete EDTA-free protease inhibitor cocktail (Roche) and 10 μg/ml DNAseI (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... 5 µL LightCycler 480 SYBR Green I Master Mix (Roche Applied Science) and 1 µL (3 µM ...
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bioRxiv - Neuroscience 2020Quote: ... Then embryos were incubated for 2 hours in 5% Blocking Reagent (Roche) in MAB (150 mM maleic acid ...