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Citations for Roche :
2251 - 2300 of 2542 citations for Mouse anti Rift Valley Fever Virus Nucleoprotein M977 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Pellets were resuspended in buffer (50mM Tris-HCl pH 7.0, 100 mM NaCl, and 1 tablet anti-protease cocktail (Roche) for 25 mL) ...
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bioRxiv - Biochemistry 2019Quote: ... CaCl2 was added to a final concentration of 1.25 mM and incubated with 200 μl Anti-Protein C Affinity Matrix (Roche) for 4 hrs while rotating at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C in B1 buffer with alkaline phosphatase-conjugated anti-DIG (1/2000; Roche 11633716001). After three washes in B1 buffer and one wash in B3 buffer (100 mM Tris–HCl pH 9 ...
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bioRxiv - Genomics 2021Quote: ... Samples were then incubated overnight at 4°C with a rabbit anti-DIG HRP-conjugate antibody (1:500, Roche). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with 1:2000 alkaline phosphatase-conjugated anti-DIG antibodies (Sigma/Roche, 11093274910). After washes and prior to development ...
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bioRxiv - Developmental Biology 2021Quote: ... 20% heat-inactivated lamb serum in Tris-buffered saline with 0.1% Tween-20 (TBST) and incubated with alkaline phosphate-conjugated anti-DIG antibody (1:2000, Roche) in blocking buffer overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2hr at room temperature and then incubated in blocking solution containing anti-Digoxigenin-AP antibody (1:2000; Roche) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The isotypes of anti-Drosophila antibodies were determined by the IsoStrip™ Antibody Isotyping Kit (11493027001 Roche, Basel, Switzerland) according to the instructions of the manufacturer.
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Neuroscience 2021Quote: ... Tween-20 0.3%) for 1 hour and finally incubated overnight at 4°C with anti-Dig-POD antibody (Roche) diluted 1:500 in blocking solution ...
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Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafishbioRxiv - Developmental Biology 2022Quote: ... The probes labeled by digoxigenin were detected using alkaline phosphatase coupled anti-digoxigenin Fab fragment antibody (Roche, Basel, Switzerland) with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... samples were lysed in 1X RIPA lysis buffer containing anti-protease cocktail (cOmplete™, Mini Protease Inhibitor Cocktail, Roche) along with denaturing buffer supplied by NEB ...
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bioRxiv - Neuroscience 2022Quote: ... All co-cultures were fixed and post-fixed (before and after incubation with primary rat anti-HA antibody (Roche), respectively ...
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bioRxiv - Plant Biology 2023Quote: ... except that 2 g of fresh weight whole seedlings were harvested for each genotype (3 biological replicates each) and 1.5 ug of anti-HA 3F10 (Roche) antibody were used for immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was incubated at 4°C overnight with 20 μl anti-HA antibody coated beads (Roche, Rotkreuz, Switzerland) at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat. No. 11814460001) bound on protein G agarose ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was incubated with the probe overnight and Arc positive cells were detected with anti-digoxigenin-HRP conjugate (Roche Applied Science Ref # ...
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were then incubated with the anti-myc antibody (11667149001 9E10 mAb from Roche; 1:250 in blocking buffer) for two hours at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... and then total proteins were extracted and protein levels were determined by immunoblot analysis with anti-GFP antibody (Roche).
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bioRxiv - Molecular Biology 2024Quote: ZNF274 expression was induced with 1µg/ml doxycycline and verified via western blot with an anti-HA antibody (1:1000, ref.12013819001, Roche) and anti-beta actin antibody (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: Antibodies used for western blots presented in this work were as follows: anti-HA monoclonal antibody (mAb) 3F10 (diluted 1:2,000) (Roche); mouse anti-GAPDH mAb (1:20,000).
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bioRxiv - Microbiology 2024Quote: ... with their respective dilution in 5% skimmed milk in PBS-tween 0.1% were used: anti-HA-HRP (3F10) (N°12013819001, Roche) 1/10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were blocked using non-fat dry milk and stained with an anti-GFP primary (Roche; 1:1000) and a goat anti-mouse secondary antibody coupled to horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with anti-digoxigenin-alkaline phosphatase antibodies (1:5000; Roche; 11093274910; AB_514497) diluted in blocking solution ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... 100mM sodium chloride) for at least 2 hours and then incubated in anti-fluorescein-POD Fab fragments (Roche, 11207733910) diluted at 1:400 overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed using alkaline phosphatase-conjugated anti-digoxigenin Fab fragments and the chemiluminescent substrate CSPD (Roche Applied Science). ImageJ analysis was performed to calculate the relative intensity of Maxi-probe detected bands with the corresponding control-probe detected bands.
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bioRxiv - Developmental Biology 2023Quote: ... Sense and anti-sense riboprobes were generated using a digoxygenin RNA labelling kit and T3/T7 RNA polymerases (Roche). Images are representative of at least 3 embryos per condition.
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bioRxiv - Plant Biology 2023Quote: ... Western blotting was conducted as described above and visualized with anti HA-HRP antibody (Roche 39 #12013819001; 1:2,000).
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated overnight at 4°C: rabbit anti-HA high affinity at 1:500 (Roche Diagnostics #ROAHAHA), mouse anti-FLAG M2 at 1:500 (Sigma-Aldrich #F1804) ...
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bioRxiv - Developmental Biology 2023Quote: ... In situ hybridization was performed as previously described19 with custom anti-sense DIG-labeled riboprobes transcribed in vitro (Roche) from amplified cDNA (see Key Resources Table for primers) ...
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bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
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bioRxiv - Biochemistry 2023Quote: ... the Dig at the end of the DNA-bead conjugate was first blocked with anti-digoxigenin Fab fragment (Roche) in a ratio of 1:2 respectively in Buffer C with 7 mM MgCl2 ...
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bioRxiv - Biophysics 2023Quote: ... Applying voltage to the piezo element produced standing planar acoustic waves generating force applied to the polystyrene microspheres inside the chamber in the direction towards the top side of the chamber The AFS chip was first passivated by treatment with 0.05 mg/ml of anti-digoxigenin antibodies (Roche), followed by incubation with 0.2% BSA (Amresco ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG/HA-AGO2 expression and immunoprecipitation efficiency were determined by Western blot using anti-HA antibody (clone 3F10, Roche). RNA was extracted by adding Trizol reagent (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with horseradish peroxidase (HRP)-conjugated anti-DIG (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010 ...
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bioRxiv - Microbiology 2023Quote: ... Immune complexes were separated with SDS-PAGE and then Western blotted with rat anti-HA antibody (clone 3F10, Roche), or rabbit anti-TgSkp1 affinity purified polyclonal antibody UOK75 [76],
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bioRxiv - Plant Biology 2024Quote: ... The eluted proteins were separated on 8% SDS-PAGE gel (37.5:1 acrylamide:bis-acrylamide) and probed with anti-HA (Roche, 3F10), anti-GFP (Abcam ...
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bioRxiv - Plant Biology 2024Quote: ... pH 7.5) and probed for 30 min with an alkaline phosphatase-labeled anti-digoxigenin antibody (Roche, catalog no. 11093274910). Membranes were washed twice for 15 min with washing buffer (0.1 M maleic acid ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then blocked for 1 h and incubated with an anti-DIG antibody (Roche 11093274910, RRID:AB_514497, 1:2000) diluted in MABT buffer (0.1 M maleic acid pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... positive cells in the human biopsies but with the visualization systems (Omni-Map anti-Rabbit) conjugated to horseradish peroxidase developing the reactions with the 3,3’-diaminobenzidine tetrahydrochloride (ChromoMap DAB, Ventana, Roche) and purple chromogen (Discovery Purple Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... Dicer was detected using anti-HA 3F10 monoclonal primary antibody (High Affinity rat IgG1, Roche #11867431001; dilution 1:500), anti-HA rabbit primary antibody (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... post-nuclear lysates pre-cleared using CL-4B Sepharose beads followed by immunoprecipitation with anti-HA-antibody (12CA5) and Protein G agarose (Roche). Beadbound radiolabelled substrates were resuspended in 2 x Laemmli buffer (+20 mM DTT) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with blocking buffer (4 % skim milk in 1 × PBS) followed by incubation with the primary antibody: (monoclonal anti-GFP mice 1:5000 (Roche), rabbit anti-GFP 1:10000 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were blocked in 10% inactivated sheep serum for 1 h followed by overnight incubation with 1:1000 anti-digoxygenin (DIG) antibody (Roche). The sections were washed in PBT and incubated with NBT/BCIP (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or fluorescein-labeled riboprobes were synthesized as described (Pearson et al., 2009) and detected with anti-digoxigenin-HRP (1:2000, Roche/Sigma-Aldrich 11207733910 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Maltose binding protein/hemagglutinin-tagged Nuku proteins were detected using an anti-HA peroxidase-conjugated monoclonal rat antibody (3F10; 12013819001 (Roche)).
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bioRxiv - Evolutionary Biology 2020Quote: ... sections were incubated in a 1:1000 dilution of alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics, Cat. No. 11207733910) then stained with nitro blue tetrazolium (Roche Diagnostics ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Colorimetric whole mount in situ hybridisation (NBT/BCIP) was performed as previously described using anti-DIG antibody conjugated with Alkaline-Phosphatase (Roche) (Schinko et al ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4°C with anti-Digoxigenin antibody coupled to alkaline phosphatase (Roche, 11207733910, used at 1/4000). The last day ...