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Citations for Roche :
2201 - 2250 of 6348 citations for HDM Fluorescent Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Neuroscience 2023Quote: Hippocampi were homogenized in 500µl of RIPA medium lysis buffer (Beyotime, #P0013E-2) in the presence of protease inhibitor cocktail (Roche, #11836170001). Samples were centrifuged at 10,000 × g for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: Cell lysate powder from 12 L of cell culture was resuspended in 300 mL of room temperature eIF4G Lysis Buffer supplemented with 2 EDTA-free protease inhibitor tablets (Roche), 20mM imidazole ...
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bioRxiv - Immunology 2023Quote: ... minced with scissors and razor blade in the presence of 1 ml of digest media (2 mg/ml collagenase IV (Roche), 1 mg/ml hyaluronidase (Worthington) ...
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bioRxiv - Molecular Biology 2023Quote: ... uteri were harvested from pregnant mice at 4.5 days post coitus and washed with cold swelling buffer (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 1X Protease Inhibitor Cocktail (PIC, Roche, 11836170001)) immediately after collection ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
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bioRxiv - Microbiology 2024Quote: ... and lysed by incubation with nondenaturing lysis buffer (300 mM NaCl, 100 mM Tris-HCl [pH 7.4], 2% Triton X-100, and 1×EDTA-free protease inhibitor cocktail [Roche Complete]) for 30 min on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were then blocked in blocking solution (maleic acid buffer containing 2% Boehringher-Mannheim blocking reagent) and incubated overnight at 4°C in blocking solution containing anti digoxigenin antibody (Roche) diluted at 1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... colorimetric reaction was performed for 1-2 days at room temperature in a solution containing NBT (nitro-blue tetrazolium chloride, Roche) and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed in sterile-filtered PBS (pH 7.2) and resuspended in sterile-filtered ice-cold FACS buffer (2% (w/v) bovine serum albumin fraction V (Roche, Switzerland) and 0.01% sodium azide (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1% Cholesteryl Hemissucinate (CHS) (Avanti Pola lipids) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... 200 mM NaCl and 0.5% SMALP200 (CubeBiotech GmbH) supplemented with 2 tablets of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). The resuspended pellet was homogenized manually in a 40 ml Kimble glass homogenizer (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... 2 ng of cDNA were amplified per reaction and each reaction was performed in triplicate using the LightCycler 384 (Roche) with SYBR Green Master Mix II (Roche) ...
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bioRxiv - Immunology 2024Quote: ... digested three times (DMEM high glucose with 2% FBS, 100 µg/ml DNase I, and 100 µg/ml Liberase TM, Roche) or until no tissue fragments remained ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with sgRNA expression vectors and lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 2:1:1 ratio using X-tremeGENE 9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA and 1% Triton X-100) containing 2 mM Na3VO4 (Sodium Orthovanadate) and 1 mM pmsf (phenylmethylsulfonyl fluoride) and 1x protease inhibitor cocktail tablet (Roche). Whole cell lysates were centrifuged at 12,000 rpm at 4 0C for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... with probe prepared by nick translation of a double-stranded PCR product bearing the bcd ORF using alkali-stable digoxigenin-11-2’-deoxyuridine-5’-triphosphate (Roche), visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Microbiology 2024Quote: In-situ detection of DNA fragments following treatment of cells with MLT 5 μM and 10 μM for 24 h was measured by TUNEL using a Cell Death Detection kit (In-Situ Cell Death Detection Kit, POD; Roche; Cat. No. 11 684 817 910). 1000 μL of cells were centrifuged (3000 rpm for 5 min) ...
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bioRxiv - Microbiology 2020Quote: ... and further purified using the High Pure RNA Isolation kit (Roche). RNA quantity was assessed using the Quant-iT RiboGreen RNA Assay kit (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were constructed using the KAPA HyperPrep Kit (Kapa Biosystems, Switzerland) followed by sequencing on an Illumina NovaSeq6000 with 2×150 bp paired-ends.
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bioRxiv - Molecular Biology 2020Quote: ... Fibroblast proliferation was determined using colorimetric BrdU incorporation assay kit (Roche) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Assays were performed using the Luciferase Reporter Gene Assay Kit (Roche) to obtain data as relative light units (RLU) ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequent ligation (Rapid DNA Ligation Kit, Roche, Merck, Zug, CH). Then ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions with KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems) were setup with 80ng genomic DNA ...
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bioRxiv - Genomics 2020Quote: ... Exome libraries were prepared using a Hyper Prep Kit (KAPA Biosystems), pre-capture PCR and SureSelectXT Human All Exon V5+UTR baits (Agilent) ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNA was isolated using a KAPA Mouse Genotyping Kit (Roche). PCR amplification of the N-terminal region of TRIM1 was performed using Q5 High-Fidelity 2X Master Mix (New England BioLabs ...
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bioRxiv - Developmental Biology 2021Quote: ... The In Situ Cell Death Kit (TMR red; Roche Applied Science) was applied to fixed retinal sections as per the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Library concentration was determined using a KAPA Library Quantification Kit (Roche). Deep sequencing was performed on an Illumina NovaSeq 6000.
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bioRxiv - Genomics 2022Quote: ... two libraries were constructed using the Kapa Hyper Prep kit (Roche) according to Arima’s recommendation (Library Preparation using KAPA Hyper Prep Kit DOC A160108 v01) ...
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bioRxiv - Cell Biology 2019Quote: ... immunofluorescence and TUNEL assay (in situ cell death detection kit, Roche). Images were captured by Aperio (Leica Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP library was constructed by KAPA Hyper Prep Kit (KAPA BIOSYSTEMS) and SeqCap Adapter Kit A (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared using the KAPA Library Preparation Kit (KAPA Biosystems), and were amplified for a total of 12 PCR cycles (15 s at 98°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and quantification performed with a KAPA Library Quantification Kit (Roche, KK4844). Libraries were sequenced by the Babraham Institute Next Generation Sequencing Facility on an Illumina HiSeq2500 in Rapid Run 50bp Single End mode.
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bioRxiv - Genomics 2020Quote: ... with KAPA Library Quantification Kit (Kapa Biosystems Inc., Wilmington, MA, USA) for concentration ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were constructed using the KAPA Hyper Prep Kit (Roche) or NEBNext UltraII DNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Immunology 2019Quote: ... with the Light Cycler 480 SYBR Green detection kit (Roche Diagnostics) using the primers listed in Supplementary Table 2 ...
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bioRxiv - Physiology 2019Quote: ... and the DISCOVERY ChromoMab DAB kit (RUO) (Ref. 760-159, Roche). Sections were incubated with the primary antibody anti-Pecam1 (Ref ...
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bioRxiv - Genomics 2019Quote: ... and quantity measured with KAPA Library Quantification Kits (KK4835, KAPA Biosystems).
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bioRxiv - Genomics 2019Quote: ... and quantity measured with KAPA Library Quantification Kits (KK4835, KAPA Biosystems)
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bioRxiv - Microbiology 2019Quote: ... TUNEL-Fluorescein (In Situ Cell Death Detection Kit, Roche Life Science) was used to measure apoptosis at the single cell level following manufacturer’s recommendations ...
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bioRxiv - Physiology 2019Quote: RNA was extracted using the High Pure RNA Tissue Kit (Roche) following the manufacturer’s guidelines and the concentration determined using the Nanodrop 1000 spectrophotometer (ThermoScientific) ...
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bioRxiv - Physiology 2019Quote: ... RNA was extracted using the High Pure RNA Tissue Kit (Roche) following the manufacturer’s guidelines and the concentration determined using the Nanodrop 1000 spectrophotometer (ThermoScientific) ...
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bioRxiv - Genomics 2019Quote: ... and quantified with the KAPA Library Quantification Kit (Kapa Biosystems #KK4844), and then sequenced in a paired-ended manner using the NextSeq 500 (Illumina ...
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bioRxiv - Immunology 2019Quote: ... library preparation was performed using the Kappa Hyperprep kit (Roche Diagnostics) and sequencing was undertaken by the Pathogens Genomic Unit (UCL ...
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bioRxiv - Immunology 2019Quote: ... and quantified with Kapa Library Quantification Kit (Kapa Biosystems, Wilmington, MA). Libraries were sequenced by paired-end sequencing (2 × 150bp ...
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bioRxiv - Synthetic Biology 2019Quote: ... using the Kapa library quantification kit for Illumina platforms (Kapa Biosystems). The libraries were then pooled at equimolar concentrations and sequenced on the Illumina MiSeq platform at a read-length of 300bp paired-end ...
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bioRxiv - Microbiology 2019Quote: ... DNA was purified using High Pure PCR Product Purification Kit (Roche), according to the recommendations of the manufacturer ...
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bioRxiv - Neuroscience 2019Quote: ... and quantified using KAPA Library Quantification Kit for Illumina (KAPA Biosystems). Paired end 75 bp reads were obtained for pooled libraries using Illumina HiSeq 2500 at the JHU GRCF High Throughput Sequencing Center.
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bioRxiv - Microbiology 2019Quote: ... the TUNEL assay was performed with TUNEL Apoptosis Assay Kit (Roche). Briefly ...