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Citations for Roche :
2201 - 2250 of 8032 citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 0.5 mM DTT and 0.5% Triton X-100 (pH 7.5) supplemented with complete protease inhibitor cocktail tablets (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... Insect cell lysates containing expressed untagged Cdc28 and Cks1 were prepared as described above in lysis buffer (50 mM HEPES, pH 7.5, 150 mM KCl, 5 % glycerol, 0.01% Tween and complete EDTA-free protease inhibitors [Roche]) and the cleared lysates were used to assemble the trimeric CDC28 complex with 1xStrep-Clb2 purified from E ...
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bioRxiv - Cell Biology 2022Quote: ... The single strand DNA oligo was first labelled with Digoxigenin at 5’-end using end labelling kit (Roche) as per the manufacturers’ protocol ...
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bioRxiv - Neuroscience 2019Quote: ... 12 strokes, 900 rpm) using homogenizer buffer (0.32 M Sucrose, 5 mM HEPES pH 7.4, Protease inhibitor cocktail (Roche)) ...
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bioRxiv - Immunology 2021Quote: ... the cells were thawed on ice followed by lysis using Farham lysis buffer (5 mM PIPES pH 8.0, 85mM KCl, 0.5% NP-40 supplemented with protease and phosphatase inhibitors (Roche)) made in miliQ ...
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bioRxiv - Neuroscience 2020Quote: ... the alkaline phosphatase activity was detected by using nitroblue tetrazolium chloride (337.5μg/ml) and 5-bromo-4-chloro-3-indolyl phosphate (175μg/ml) (Roche Diagnostics). Sections were mounted in Mowiol.
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bioRxiv - Neuroscience 2020Quote: ... participants completed five 5-min trials of a motor-response inhibition task (Garavan et al., 1999, 2002; Roche et al. ...
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bioRxiv - Neuroscience 2020Quote: ... with 0.34 mg/ml nitroblue tetrazolium (NBT) and 0.35 mg/ml 5-bromo-4-chloro-3indolyl-phosphate (BCIP; Roche), for 1–12 hours at room temperature with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were blocked in Blocking Buffer (5% sheep serum with 10% Roche Blocking Reagent 10x in MABT) for 1 hr at RT and incubated overnight with anti-Digoxigenin-AP (1:4000 ...
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bioRxiv - Genomics 2022Quote: ... 5 µL of 20 mM siRNAs and 3 µL of X-tremeGENE HP DNA Transfection Reagent (Roche, #6366546001) were diluted in 200 µL Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Tissue was directly lysed in 2x Laemmli Sample Buffer containing 5% β-ME and a PhosSTOP tablet (Roche), 10 ul per embryo ...
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bioRxiv - Genomics 2020Quote: ... The 2nd strand was synthesized using 2nd primer (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNGTGGTATCAACGCAGAGTAC -3) with KAPA HiFi HS mix (KAPA Biosystems). The double stranded cDNAs were amplified using Illumina adapter-specific primers and LongAmp Taq DNA polymerase (NEB) ...
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bioRxiv - Immunology 2019Quote: ... C57BL6 mice were treated intravenously with either vehicle (0.9% NaCl sterile saline) or Pulmozyme (5 mg/kg, Roche) 1 hour before infection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL DNA (10 ng/μL) were amplified using Lightcycler® FastStart DNA Master HybProbe Mix (Roche, Germany) and the respective LightSNiP Assay (TIBMolBiol ...
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bioRxiv - Cancer Biology 2019Quote: ... Flp-In 293 cells were first washed with PBS and collected in homogenization buffer (250 mM sucrose, 5 mM Hepes, pH 7.4, 0.5 mM EGTA, and Protease inhibitor cocktail (1187358001, Roche), then homogenized in a glass/glass ...
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bioRxiv - Neuroscience 2020Quote: The PCR reactions were performed with 5 μL of LightCycler® 480 High Resolution Melting Master (Roche, #04909631001), 0.5 μL of each primer (10 μM) ...
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bioRxiv - Genomics 2019Quote: ... fragments were allowed to anneal and extend for 5 cycles with KAPA HiFi 2X HotStart Readymix (Kapa Biosystems) before primers HSSF-ATGC and DO_95R were added for amplification (Supplemental Table 8).
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bioRxiv - Developmental Biology 2019Quote: ... and stained with standard Nitro Blue Tetrazolium (NBT) and 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) (Roche) ISH protocol ...
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bioRxiv - Genomics 2021Quote: ... DNA was prepared using Qiagen Large-Construct Kit and labelled with tetramethyl-rhodamine-5-dUTP (Roche Applied Sciences) by using the Nick Translation Mix (Roche Applied Sciences ...
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bioRxiv - Genomics 2021Quote: ... DNA was prepared using Qiagen Large-Construct Kit and labelled with tetramethyl-rhodamine-5-dUTP (Roche Applied Sciences) by using the Nick Translation Mix (Roche Applied Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei isolated from approximately 2.5x107 cells were resuspended in one ml of extraction buffer (50 mM TrisHCl pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM DTT, 0.25%NP-40, 1x Roche Complete protease inhibitors ...
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bioRxiv - Developmental Biology 2020Quote: ... nuclei were harvested using cell lysis buffer (5 mM PIPES, pH 8.0, 85 mM KCL, 0.5% NP40, and Roche cOmplete ...
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bioRxiv - Developmental Biology 2020Quote: ... with some dams bearing 13.5 dpc embryos also intraperitoneally injected with 5-Bromodeoxyuridine (BrdU, Roche, 40 mg/kg) to track cell division ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries from 5-10 ng of ChIP DNA was prepared using the KAPA HTP Library Preparation Kit (Roche) by the Next-Generation Sequencing Core at UT Southwestern ...
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bioRxiv - Immunology 2020Quote: ... washed twice with PBS and lysed in 5 ml lysis buffer (50 mM Tris-HCL, ph7,4; 500 mM NaCl, 0,2% SDS; 1x protease inhibitors (Roche), 20 mM DTT ...
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bioRxiv - Molecular Biology 2022Quote: ... The isolated nuclei were warmed at 37oC for 5 min and treated with micrococcal nuclease (MNase) (Roche 10107921001) and 2 mM CaCl2 (RICCA R1760000) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were lysed with 5mL cold lysis buffer (10 mM Tris-HCl, pH 7.5; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; 1x complete protease inhibitor; 11836145001 Roche) for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP, 20% glycerol, 5 mM imidazole, 1.4 μg/mL leupeptin, 1.0 μg/mL pepstatin A and two Roche EDTA-free protease tablet per 5 g cell pellet) ...
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bioRxiv - Molecular Biology 2023Quote: ... DPCs were resolved on 5-18% SDS-PAGE gradient gels and transferred to a PVDF membranes (Roche, 03010040001). Membranes were blocked with 5% low-fat milk in TBST buffer (10mM TrisHCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.2 g of powder was dissolved in 1.2 ml of microsome buffer (50 mM MOPS, 0.5 M sorbitol, 5 mM EGTA, complete protease inhibitor (Roche) at pH 7.6) ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Colorimetric signals were generated using a development solution containing 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 µl gene specific primer mix (5 µl each) and 4.5 µl FastStart Essential cDNA Green Master (Roche) were amplified using 45 cycles of 25 s at 95 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 5 mM N-ethylmaleimide and Complete protease inhibitor cocktail (Roche, one tablet/10 ml of buffer). HA-tagged ubiquitin was purified using Pierce anti-HA magnetic beads (clone 2–2.2.14) ...
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bioRxiv - Bioengineering 2023Quote: ... 3 % NaCl (5 M, v/v), 0.08 % CaCl2 (2.5 M, v/v) and cOmpleteTM ULTRA Protease Inhibitor Cocktail tablets (Roche) in H2O ...
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bioRxiv - Genetics 2023Quote: Quantitative RT-PCR was performed in a final volume of 5 μl with SYBR Green I Master (Roche) and analyzed with a Lightcycler 480 (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were washed three times with 200 µL of washing buffer (50 mM Tris-HCl at pH 7.5, 150 mM KCl, 5 mM MgCl2, 0.05% NP-40, EDTA-free cOmplete mini protease inhibitor [Roche, 11836153001] ...
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bioRxiv - Cell Biology 2023Quote: ... 80 μL DNase I (5 mg/ml; DNase I – grade II from bovine pancreas from Roche (Basel, Switzerland)) ...
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bioRxiv - Microbiology 2023Quote: ... after which they were resuspended in 1.0 ml of ice-cold lysis buffer (10 mM Tris-HCl, pH 7.7; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; protease inhibitors [Roche]) and incubated on ice for 10 min before nuclei were pelleted (400 × g ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA extracted from the parasites at passages 7 and 20 were digested with 5 U Rsa1 (Roche) at 37 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.9; 5 mM MgCl2; 0.2% Triton X-100; 20% glycerol; 300 mM NaCl and freshly added ROCHE cOmplete protease inhibitor cocktail at 0.01 tablet/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl cDNA and 5 µl of LightCycler 480 SYBR Green I Master mix (Roche Diagnostics GmbH, Germany). Three technical replicates of each sample were included ...
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bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 500 µL cold cell lysis buffer (5 mM PIPES pH8.0, 85 mM KCl, 0.5% NP-40, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 7.4, 150 mM NaCl, 1.25 mM MgCl2, 5 U/ml RNase inhibitor [Invitrogen], protease inhibitor cocktail [Complete; Roche] ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS and lysed for 20 min on ice in lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, supplemented with protease inhibitors [Roche]). Centrifugation at 13,000×g for 10 min at 4°C was then used to remove cell debris ...
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bioRxiv - Cell Biology 2021Quote: ... 1% BSA (Roche), 20% Dextran Sulfate (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 1 × PhosSTOP (Roche)] and incubated on ice for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... 1× PhosSTOP (Roche)) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1× cOmplete (Roche), and lysed by bead-beating ...
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bioRxiv - Biochemistry 2020Quote: ... 1% NP40 (Roche) and a phosphatase inhibitor mixture containing 20 mM NaF ...