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Citations for Roche :
2151 - 2200 of 3389 citations for 6 cyano 5 methoxy 12 methylindolo 2 3 a carbazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... N3500], 50 mM of Tris [pH 7.5], 150 mM of NaCl, 3 mM of MgCl2, 1X protease inhibitors [Roche, Mannheim, Germany; 05892953001]). Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... a 6.7 kb DNA fragment starting 1 kb upstream of murine Gnrhr exon 3 was amplified by PCR using the Expand Long Template PCR System (Roche, Basel, Switzerland) from 129SvEv genomic DNA using primers incorporating 5’ XmaI and 3’ NotI restriction enzyme sites (Table 1) ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Physiology 2022Quote: ... n=3/genotype) were used for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to visualize cell apoptosis/death (Roche, Basel, Switzerland). Sections were counterstained with DAPI and visualized using epifluorescence (Nikon Eclipse Ni E800) ...
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bioRxiv - Microbiology 2022Quote: ... 45 cycles of 95°C for 3 s and 60°C for 30 s using the LightCycler 96 system (Roche, Basel, Switzerland) or the StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... and 3-4 mice/group subcutaneously received 25ng/g of pegylated-human interferon α (Peg-hIFNα-2a) (Hoffmann La Roche, Basel, Switzerland). To activate IFN-1 signaling ...
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bioRxiv - Genomics 2022Quote: ... Empty/filled PCRs (combining 5′ and 3′ flanking primers) and full-length PCRs (using junction-spanning primers) were performed using an Expand Long Range dNTPack (Roche, Cat#: 4829034001). Reaction mixes contained 5μL 5× Expand Long Range Buffer with 12.5mM MgCl2 ...
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bioRxiv - Cancer Biology 2019Quote: ... MA; N3500], 50mM of Tris [pH 7.5], 150 mM of NaCl, 3 mM of MgCl2, 1X protease inhibitors [Roche, Mannheim, Germany; 05892953001]). Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Membranes were blocked for 1 h with 3% (w/v) non-fat milk powder in PBS/0.1% (v/v) Tween (PBST) (CALR, 10% Roche WBR in PBST) and incubated with primary antibody (Supplementary Table III ...
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bioRxiv - Biophysics 2019Quote: ... or for confocal in a alkaline-washed glass-bottomed chamber coated with human plasma fibronectin (25 µg/mL at 4°C overnight in 1/3 X MBS; Roche Molecular Biochemicals) in DFA supplemented with antibiotic/antimycotic (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... and prepared for shotgun metagenomic sequencing using the Kapa Hyperprep Plus kit with 3 rounds of PCR amplification (Kapa Biosystems, Wilmington, MA), and sequenced to an average depth of 7 gbp per fraction on the Illumina NovaSeq (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... qRT-PCR reactions were carried out in triplicates following a 3-step amplification protocol using the LightCycler 480 system (Roche Diagnostics, Switzerland). The ΔΔCT method [94] was used to calculate gene expression changes relative to housekeeping genes β-actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 1 mM PMSF, 3 mM ATP, 10% sucrose and Roche protease inhibitors). For sS1 constructs ...
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bioRxiv - Biochemistry 2024Quote: ... 20 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 10% sucrose, 3 mM ATP, 1 mM DTT, 1 mM PMSF and Roche Protease Inhibitors), 1 ml per dish ...
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bioRxiv - Microbiology 2023Quote: ... Abutting primer pairs were designed that were specific to these genetic groups (Supplementary Table 3) to enable the recovery of full viral genomes using polymerase chain reaction (PCR) with HiFi HotStart DNA polymerase (Kapa Biosystems, USA) using cycling conditions per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were lysed in 50 µl of lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 1× Roche Complete protease inhibitors cocktail) by pipetting up and down ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼0.5×106cells were collected and wash 3 times with Wash buffer (20 mM HEPES-KOH, pH 7.5,150mM NaCl, 0.5mM Spermidine, and Roche Complete Protease Inhibitor EDTA-free). Cells were captured with BioMagPlus Concanavalin A (Polysciences ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl, 10% sucrose, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 3 mM ATP, 1 mM PMSF and Roche protease inhibitors). Native mouse regulatory light chain (RLC ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 mM β-mercaptoethanol (BME) and 0.3 μg/ml DNase I supplemented with 600 μl of protease inhibitor cocktail (Roche, Basel, Switzerland) and 5 mg lysostaphin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... tissue sections (n = 2-3 per tissue type) underwent deparaffinization and heat-mediated antigen retrieval on the Ventana Discovery Ultra auto-stainer platform (Roche Diagnostics, Canada), following the below instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Reaction volumes were increased to 50 ul and 3 μl was subsequently used for qPCR reactions with SYBR Green (Roche, cat# 04887352001). Forward primers were designed at the 5’ ending of tRNAs and the reverse primers were designed against the 3’adapter while specificity was created against the 3’ endings ...
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bioRxiv - Genomics 2020Quote: ... washed with 500 μL buffer MB#1 (10 mM Tris-HCl, pH 7.5, 50 nM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche complete EDTA-free (Roche diagnostics ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 μl gene specific primer mix (5 μM each) and 4.5 μl FastStart Essential cDNA Green Master (Roche) were amplified using 45 cycles of 25 s at 95 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 ng or less of DNA was used for library preparation using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: Transcriptional start sites of the virD and virB transcripts were obtained by using the 5’ RACE Kit (Roche), as described (Andrade et al ...
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bioRxiv - Genetics 2019Quote: ... After 3h at 4°C slides were washed and blocked with 0.5x blocking buffer (1.5% BSA, 5% goat serum, 0.05% Triton X-100) containing protease inhibitors (Roche 1836153) for 1h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Fixed embryos were placed into hybridization buffer (50% formamide, 5×SSC, 1 mg/ml yeast tRNA (Roche, 10109223001), 100 μg/ml heparin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 5 mM MgCl2, 20 mM Tricine-KOH, 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, 1X Roche EDTA-free protease inhibitor cocktail ...
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bioRxiv - Plant Biology 2021Quote: Genomic DNA (5 μg) was used to construct the BS-seq library with the KAPA Library kit (Roche) and EpiTect Bisulfite Kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... Cell pellets were afterwards washed in 5 mM Hepes buffer supplemented with EDTA-free protease inhibitors mix (Roche), resuspended in 0.1M DTT ...
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bioRxiv - Microbiology 2020Quote: ... with RNA extraction controls—5 μL of LightMix® Modular EAV RNA Extraction Control (EAV; Roche, Basel, Switzerland) or 10 μL of MS2 phage (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the gDNA fragments were amplified using 5 rounds of PCR with KAPA HiFi 2X master mix (KAPA Biosystems). See Table S4 for primer sequences ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed again in PBS before staining for 20 minutes in DAPI (5 µg/mL, Roche 10236276001) in PBS ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked with 5% non-fat milk TBST and probed with primary anti-GFP (Roche, 1:1000) and secondary HRP- conjugated anti-mouse antibody (R&D Systems ...
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bioRxiv - Molecular Biology 2020Quote: Adherent cells were washed twice with PBS and then scraped and digested on ice for 30 min with a homemade RIPA (50 mM Tris-HCl pH 7.5, 15 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS, supplemented with Protease Inhibitors [Roche] and Phosphatase Inhibitors [Sigma-Aldrich]) ...
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bioRxiv - Microbiology 2019Quote: ... pH 7.4, 500 mM NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, and 1x complete protease inhibitor [Roche]) and sonicated ...
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bioRxiv - Cell Biology 2020Quote: ... 0.3% (v/v) Tween-20) for 5 min under agitation and next blocked in 1× blocking buffer (Roche) for 30 min at room temperature with gentle shaking ...
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bioRxiv - Cell Biology 2022Quote: ... 0.5 mM DTT and 0.5% Triton X-100 (pH 7.5) supplemented with complete protease inhibitor cocktail tablets (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... Insect cell lysates containing expressed untagged Cdc28 and Cks1 were prepared as described above in lysis buffer (50 mM HEPES, pH 7.5, 150 mM KCl, 5 % glycerol, 0.01% Tween and complete EDTA-free protease inhibitors [Roche]) and the cleared lysates were used to assemble the trimeric CDC28 complex with 1xStrep-Clb2 purified from E ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were blocked with 5% milk dissolved in TBST and probed with primary anti-HA (Roche, 1:2000), anti-Flag (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... The single strand DNA oligo was first labelled with Digoxigenin at 5’-end using end labelling kit (Roche) as per the manufacturers’ protocol ...
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bioRxiv - Plant Biology 2021Quote: ... 1.5 mM magnesium acetate] containing 5 mM dithiothreitol (DTT) and 1 × EDTA-free Complete Protease Inhibitor tablets (Roche) by tapping and inverting the tubes ...
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bioRxiv - Neuroscience 2019Quote: ... 12 strokes, 900 rpm) using homogenizer buffer (0.32 M Sucrose, 5 mM HEPES pH 7.4, Protease inhibitor cocktail (Roche)) ...
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bioRxiv - Immunology 2021Quote: ... the cells were thawed on ice followed by lysis using Farham lysis buffer (5 mM PIPES pH 8.0, 85mM KCl, 0.5% NP-40 supplemented with protease and phosphatase inhibitors (Roche)) made in miliQ ...
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bioRxiv - Neuroscience 2020Quote: ... participants completed five 5-min trials of a motor-response inhibition task (Garavan et al., 1999, 2002; Roche et al. ...
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bioRxiv - Neuroscience 2020Quote: ... with 0.34 mg/ml nitroblue tetrazolium (NBT) and 0.35 mg/ml 5-bromo-4-chloro-3indolyl-phosphate (BCIP; Roche), for 1–12 hours at room temperature with gentle agitation ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were blocked in Blocking Buffer (5% sheep serum with 10% Roche Blocking Reagent 10x in MABT) for 1 hr at RT and incubated overnight with anti-Digoxigenin-AP (1:4000 ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in homogenization buffer (0.32 M sucrose, 5 mM HEPES pH 7.4, 1 mM EDTA) containing protease inhibitor Complete (Roche) and then homogenized by multiple passing through a 0.24 mm syringe.
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bioRxiv - Molecular Biology 2020Quote: ... and finally for 5 min in a 1:100 dilution of the substrate CSPD or CDP Star (Roche) in buffer 3.
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bioRxiv - Cell Biology 2019Quote: ... Tissue was directly lysed in 2x Laemmli Sample Buffer containing 5% β-ME and a PhosSTOP tablet (Roche), 10 ul per embryo ...