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Citations for Roche :
151 - 200 of 254 citations for St Louis Encephalitis virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche) for 2h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... HBE were processed for protein lysates and stored at -20°C (Complete Lysis-M Roche diagnostics). NET dosage was an average of extracellular dsDNA concentration (QuantiFluor ONE dsDNA System ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were prepared from zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Roche), using a manual dounce homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... the cleared lysate was added to 1 mL cOmplete His-Tag Purification Resin (COHISR-RO, Roche) that had been pre-washed with buffer ...
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bioRxiv - Microbiology 2023Quote: ... the pocks positive for both BleCherry and GFP signals (pocks containing the recombinant green/red virus) were isolated for DNA extraction using High Pure Viral Nucleic Acid Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001). Cell lysates were separated by SDS-PAGE (Bio-Rad 4-20% Mini-PROTEAN TGX Precast Gel ...
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bioRxiv - Cell Biology 2020Quote: ... All lysates included protease and phosphatase inhibitors at the recommended concentration (Complete-EDTA-free and PhosSTOP [Roche]).
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bioRxiv - Immunology 2021Quote: Macrophage cell lysates were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor cocktails and PhosSTOP (Roche). Protein concentration was measured using the BCA Protein Assay Kit (Pierce) ...
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bioRxiv - Systems Biology 2020Quote: ... Diluted lysates were then used directly in qPCR with either Kapa 2x MasterMix Universal (KK4602, Kapa Biosystems) or Power SYBR Green Master Mix (4368706 ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell lysate (in Buffer C supplemented with a cocktail of protease inhibitors (cOmplete, EDTA-free (Roche)) ...
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bioRxiv - Microbiology 2020Quote: Protein lysates of HEK-293T cells were extracted with RIPA buffer (PIERCE) and protease inhibitor cocktail (Roche). Loading buffer (Life Technologies ...
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bioRxiv - Biophysics 2022Quote: ... and 500-750 μg lysate was pre-cleared with Protein G agarose beads (Roche; Cat. No.11719416001) for 1 hour at 4°C with rotation at 5 rpm in a rotator ...
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bioRxiv - Molecular Biology 2022Quote: Cell lysates were prepared in radioimmunoprecipitation assay buffer supplemented with protease inhibitor cocktail tablets (Roche, Basel, Switzerland), and total protein was measured using a Bicinchoninic Acid Protein Assay Kit (Beyotime Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: Cell lysates were prepared in 1x PXL lysis buffer supplemented with complete protease inhibitors (Roche, cat. #11873580001). All lysates were prepared from equal cell numbers per condition ...
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bioRxiv - Microbiology 2023Quote: Cell lysates were prepared in lysis buffer containing 1% Nonidet P-40 and protease inhibitor cocktail (Roche) (Cao ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C) the lysate was transferred to 50 μl HA bead slurry (anti-HA affinity matrix, Roche) or 30 μl c-myc bead slurry (EZview red anti-c-myc affinity gel ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were collected in RIPA buffer supplemented with proteinase and phosphatase inhibitors (Roche 11697498001, Roche 04906937001). Protein was separated by electrophoresis using 4- 12% gradient Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were collected in RIPA buffer supplemented with proteinase and phosphatase inhibitors (Roche 11697498001, Roche 04906937001). Protein was separated by electrophoresis using 4- 12% gradient Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...
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bioRxiv - Biochemistry 2022Quote: ... and 10% glycerol] containing the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche). The lysate was frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Cell Biology 2022Quote: ... cell lysate was incubated with agarose beads conjugated with either mouse IgG or mouse anti-GFP antibodies (Roche) for 3h at 4°c under gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from cell lysates which were previously lysed by external lysis buffer (Roche, Penzberg, Germany) as described above ...
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bioRxiv - Cancer Biology 2020Quote: ... lysates were prepared in RIPA lysis buffer supplemented with complete ULTRA protease inhibitor cocktail tablets (Roche, Basel, Switzerland) and western blotting was performed as demonstrated before (Nair et al. ...
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bioRxiv - Cell Biology 2021Quote: ... whole cell lysates were obtained by lysing the cells in RIPA buffer containing cOmplete Protease Inhibitor Cocktail (Roche). Following sonication ...
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bioRxiv - Molecular Biology 2021Quote: Lysates for Western-blot were made in a 1% triton buffer including cOmplete protease inhibitor cocktail (Roche 11697498001) and Western-Blot and flow cytometric staining was performed as described previously (41),(42) ...
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bioRxiv - Molecular Biology 2022Quote: ... The nuclear lysates were diluted 2 fold with IP buffer (20mM HEPES, pH 7.9, 0.2M NaCl, complete protease inhibitors (Roche) and then flag-beads (Anti-Flag Magnetic Beads ...
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bioRxiv - Cell Biology 2023Quote: Whole cell lysates were prepared via scraping cells in PBS containing protease inhibitors (Complete Protease inhibitor cocktail, Roche). Cells were pelleted and resuspended in hot SDS lysis buffer (0.5 % SDS ...
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bioRxiv - Synthetic Biology 2023Quote: ... We incubated 5μl of clarified crude lysates containing rAAV particles with 50 units (U) of DNAse I (Roche) for 15hr at 37°C ...
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Salmonella actively modulates TFEB in murine macrophages in a growth-phase and time-dependent mannerbioRxiv - Cell Biology 2023Quote: ... whole cell lysates were prepared using 2x Laemmli buffer containing phosSTOP and protease inhibitor cocktail (Roche, Mississauga, ON). Lysates were passed through a 27-gauge needle ten times ...
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bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
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bioRxiv - Pathology 2024Quote: Cholesterol and triglyceride levels were quantified in liver protein lysates and EDTA-plasma using enzymatic colorimetric assays (c.f.a.s. cobas, Roche Diagnostics) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates from mouse tissues were prepared in RIPA buffer (Beyotime, P0013B) containing Protease Inhibitor Cocktail (Roche, 11873580001) and 1mM PMSF ...
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bioRxiv - Developmental Biology 2024Quote: ... ST-HSCs and LT-HSCs using a KAPA RNA HyperPrep kit with RiboErase (HMR) (Kapa Biosystems) and sequenced using a Hiseq 1500 (Illumina ...
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bioRxiv - Cell Biology 2019Quote: ... Beads were removed by centrifugation (twice at 1,000 xg) and then pre-cleared lysates were supplemented with anti-HA high affinity monoclonal antibody (clone 3F10, Roche) at 2 µg/ml final concentration along with 20 µl Protein G Sepharose beads and incubated at 4° C for 4 hours on a rotating wheel ...
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bioRxiv - Molecular Biology 2019Quote: Parasite were isolated from rings and early trophozoite stages and lysates were prepared in 1X PBS containing 0.5% TritonX-100 and protease inhibitors (Roche) by sonication (amplitude 30 ...
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bioRxiv - Microbiology 2021Quote: ... 200 μg each lysate was pre-cleared with 20 μl of Protein A agarose beads (3 mg/ml, Roche) in 200 μl for 1 hour at 4°C on a Labquake Rotator (Barnstead Thermolyne) ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The protein lysate was electrophoresed into 6-10% SDS-PAGE gels and transferred to a PVDF membrane (Roche, 03010040001). After blocking with 5% BSA ...
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bioRxiv - Genomics 2020Quote: ... Protein lysates were prepared by manually homogenizing frozen tissue in RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche). Proteins were separated on a 4-20% TGX SDS-PAGE gel (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... except that 5 mg of synchronized HeLa cell lysate in 8 M Urea with protease inhibitor cocktail (Roche, 4693116001) was diluted by PBS buffer to a final urea concentration of 1 M before incubating with the conjugated beads ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat. No. 11814460001) bound on protein G agarose ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared in radio-immunoprecipitation assay (RIPA) buffer supplemented with PhosSTOP and complete protease inhibitor cocktail (Roche). Protein concentration was determined by Bradford assay using the Protein Assay Dye Reagent Concentrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... or His6 (full length mouse MLKL and pseudokinase domains) tags and captured from lysates using glutathione resin (UBP Bio) or HisTag Ni-NTA resin (Roche) respectively ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of the post-nuclear supernatant was saved as the input lysate and 450 μL was incubated with 5μg of anti-GFP antibody (Roche 11814460001) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... cell lysates were retrieved in 1% sodium deoxycholate (SDC) in phosphate buffered saline solution with Protease Inhibitor Cocktail (cOmplete, Roche) buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were prepared as previously described (Plate et al., 2016) in RIPA buffer with fresh protease inhibitor cocktail (Roche). Total protein concentration in cellular lysates was normalized using the Bio-Rad protein assay ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ml of the lysates prepared with IP extracation buffer from 0.5 g of leaves was incubated with anti-HA Affinity Matrix (Roche 11815016) for o/n in a rotary shaker at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: Cell lysates were prepared in 2× Laemmli loading buffer supplemented with cOmplete protease inhibitor and PhosSTOP phosphatase inhibitor tablets (Roche), 1 mM PMSF ...