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Citations for Roche :
51 - 100 of 254 citations for St Louis Encephalitis virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Lysate was resuspended in 2x protease inhibitor solution (cOmplete mini, Roche) and centrifuged for 10 min at 14,000 rpm and 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were pre-cleared with protein A-agarose beads (Roche) for 3 h and then incubated with anti-β1-integrin antibodies (MAB2000 from Millipore ...
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bioRxiv - Zoology 2021Quote: ... was determined from the cell lysate using an ELISA kit (Roche) in accordance with the company instructions ...
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bioRxiv - Cancer Biology 2021Quote: RLT Plus lysates with Complete Protease Inhibitor (Roche, catalog no. 11836170001) homogenized as described in the ‘RNA sequencing’ section were passed through the RNA binding column from the AllPrep DNA/RNA Mini kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared using cOmplete™ Lysis-M (4719956001, Roche) with protease and phosphatase inhibitors and subjected to standard electrophoresis and transfer as previously described41 ...
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bioRxiv - Microbiology 2023Quote: ... phage lysates were treated with RNase A (Roche; 1 μg/mL), DNase I (NEB ...
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bioRxiv - Immunology 2023Quote: ... except cleared lysates were incubated with anti-HA matrix (11815016001, Roche) (pre-blocked with 1% BSA-PBS for 1h ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate was incubated for 30 min on ice with DNAseI (Roche) and ultracentrifuged at 120 000 g / 4°C / 40 min ...
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bioRxiv - Microbiology 2020Quote: ... Virus RNA was extracted from 400 µl of filtered supernatant with the High Pure Viral RNA Kit (Roche). The sequencing library was constructed using Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Virus-infected cells were lysed in RIPA buffer containing a protease inhibitor cocktail (cOmplete; #11697498001, Roche, IN, USA). Nuclear and cytoplasmic fractions of mHat9d cells were performed using the NE-PER Nuclear and Cytoplasmic Extraction reagents (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Screen for virus progeny production was done with SARS-CoV-2 antigen quick-test (Roche, 9901-NCOV-01G) (or CPE in E2T ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was applied to cOmplete His-Tag purification resin (Roche), pre-equilibrated in lysis buffer and incubated at 4 °C for 6 h on a tube roller ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified lysate was incubated with RNAse T1 (20 U/μL, Roche), RNase A (20 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Cleared lysates were subjected to immunoprecipitation using anti-GFP antibody (Roche 11814460001) conjugated to protein G magnetic beads (Thermofisher ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was nutated with 200 μL of Ni-NTA resin (Roche cOmplete™ His-Tag purification resin ...
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bioRxiv - Molecular Biology 2021Quote: ... the lysates were treated with RNase-free DNase I (10 U, Roche) for 30 min at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were prepared in lysis buffer supplemented with protease inhibitors (Roche) as described previously ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were prepared in RTK lysis buffer with protease (11836153001, Roche) and phosphatase (04906837001 ...
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bioRxiv - Immunology 2022Quote: Cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Roche) and PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... Clarified lysate was loaded onto a cOmplete His-tag purification column (Roche), immobilized proteins washed with lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... cell lysates were prepared in the presence of cOmplete cocktail (11873580001, Roche) and 20 mM N-ethylmaleimide (NEM ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei lysates (in Nuclear Buffer Lysis 3, supplemented with protease (Roche, 11697498001) and phosphatase inhibitors (1 mM Na3O4V (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... Sequence datasets obtained with and without VirBaits treatment were analyzed via reference mapping using a reference genome of the respective virus and the Genome Sequencer software suite (versions 2.6; Roche) to directly compare the containing virus amount in terms of virus reads of the datasets ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from 200 µl of virus-containing cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Successful virus rescue was monitored with SARS-CoV-2 Rapid Antigen Test (Cat# 9901-NCOV-01G, SD BIOSENSOR, Roche) by applying 100 μL of unfiltered culture supernatant directly onto the test device.
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bioRxiv - Molecular Biology 2021Quote: ... Titers of purified virus preparations were determined by quantitative PCR using a Roche Lightcycler 480 (Roche Applied Sciences, Pleasanton, CA) with primers amplifying the AAV2 ITR regions (forward ...
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bioRxiv - Biophysics 2020Quote: HeLa cells were plated in 6-well plates and infected with virus at equal amounts of p24 after treatment with DNaseI (Roche) for 30 min at 37°C ...
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bioRxiv - Microbiology 2019Quote: One-step simplex real-time quantitative RT-PCR amplifications were performed using Multiplex RNA Virus Master Kit (Roche Diagnostics, France) for influenza A ...
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bioRxiv - Immunology 2020Quote: ... Titers of purified virus preparations were determined by quantitative PCR using a Roche Lightcycler 480 (Roche Applied Sciences, Pleasanton, CA) with primers amplifying the AAV2 ITR regions (forward ...
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bioRxiv - Microbiology 2022Quote: A labeled DNA probe for the new virus derived from Mr was prepared using a commercial PCR DIG-labeling mix (Roche)according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cell lysates were incubated further with protein G sepharose beads (Roche Applied Bioscience) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were prepared in 1x Laemmli Buffer supplemented with Complete protease inhibitors (Roche) by heating at 37°C for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of protein lysate was incubated with 1μg anti HA-antibody (Roche) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cell lysates were collected in RIPA buffer plus inhibitors (protease: complete mini Roche; 11836153001 ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were prepared in RIPA buffer containing proteases and phosphatases inhibitors cocktail (Roche). After extracts sonication ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation and treated with RNAse A (Roche, 100 Units) or RNAse A/T1 (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... glycans were removed from lysate-derived gp120 or gp160 with Endoglycosidase H (Roche) treatment of the immunoprecipitates as described before (Land et al. ...
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bioRxiv - Immunology 2020Quote: ... β-galactosidase (lacZ) activity was measured in total cell lysates using CPRG (Roche) as a substrate ...
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bioRxiv - Neuroscience 2021Quote: ... from tissue lysates and assayed for Gli1 gene expression with SYBR-Green (Roche) on a LightCycler 480 (Roche).
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bioRxiv - Genetics 2019Quote: ... Lysates were then incubated in Eppendorf tubes with HA affinity matrix beads (Roche). Lysates were nutated for 2 hours at 4°C ...
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bioRxiv - Genetics 2022Quote: ... Worm lysates were prepared by adding RIPA buffer and proteinase inhibitor cocktail (Roche) followed by water bath sonication in a Diagenode Bioruptor XL 4 at maximum strength for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... the lysate was incubated for 1h with cOmplete His-Tag Purification Resin (Roche) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was transferred to HA bead slurry (anti-HA affinity matrix, Roche), which had been equilibrated in wash buffer (20 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... The soluble lysate fraction was incubated with rat anti-HA affinity matrix (Roche) for 3 hours at room temperature with gentle agitation ...
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bioRxiv - Genomics 2024Quote: ... The cleared lysate was loaded onto a cOmplete His-tag purification column (Roche) and the His6-Sumo3-Tn5 was eluted with a running buffer containing 300 mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... All reverse transcriptase qPCR assays for a given target were performed simultaneously for all samples using the LightCycler Multiplex RNA Virus Master Kit (Roche diagnostics). Each sample was normalized with the geometric mean of the quantification cycle (Cq ...
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bioRxiv - Microbiology 2021Quote: ... primers and probe specific for SARS-CoV-2 RdRP (TibMolBiol; #53-0777-96) and a LightCycler Multiplex RNA Virus master kit (Roche; #07083173001) according to the instructions of the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... The precleaned lysates were incubated with 1 µg Anti-HA high-Affinity (3F10, Roche) or normal IgG (GE healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... lysates were incubated at 4 °C overnight with rabbit anti-GFP (1:1000, Roche) and pull down was performed with magnetic proteinA beads (Millipore ...
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bioRxiv - Neuroscience 2019Quote: ... The protein lysates were extracted in presence of a protease cocktail tablet (Roche Diagnostics) using Cell Lysis buffer (ThermoFisher) ...