Labshake search
Citations for Roche :
151 - 200 of 415 citations for Recombinant Human CEACAM1 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were purified by affinity on cOmplete™ His-Tag Purification Resin (Roche®). Columns were washed with Buffer A followed by washing with 50 mM imidazole ...
-
bioRxiv - Cell Biology 2024Quote: ... and the supernatant incubated with 1 ml Complete His-Tag Purification Resin slurry (Roche) and Complete EDTA-free protease inhibitor cocktail tablet (Roche ...
-
bioRxiv - Biochemistry 2020Quote: ... Fractionated samples were analyzed by SDS-PAGE and electrophoresis for detection of HA-tagged Nedd4 (Roche anti-HA high affinity,1:2000 dilution ...
-
bioRxiv - Plant Biology 2022Quote: ... Tagged SAC9 fusion proteins were revealed by using GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche) and detected by chemiluminescence using ECL revelation as for T-PLATE and SH3P2 fusion proteins.
-
bioRxiv - Biochemistry 2020Quote: ... transfected with recombinant EMBacY BACs using X-tremeGENE DNA Transfection Reagent (Roche), and incubated for 72 h at 28 °C ...
-
bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and treated with DNAse I (Dnase I recombinant RNAse-free, Roche Diagnostics). Then cDNA were produced from 5 μL of total RNA using RT Superscript III (RT Superscript III First Strand cDNA Synthesis Kit ...
-
bioRxiv - Cell Biology 2020Quote: ... Genomic DNA was removed by digestion using DNase I Recombinant (Roche, 04716728001) and RiboLock RNase Inhibitor (Thermo Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... Recombinant baculovirus was generated by initial lipofection with Xtreme gene reagent (Roche) of Sf21 insect cells (Invitrogen) ...
-
bioRxiv - Neuroscience 2022Quote: ... and basic fibroblast growth factor (bFGF, 10 ng/ml; recombinant bovine, Roche). The cells were then plated in a 96-well plate in complete neurosphere medium containing DMEM/F-12 EGF and bFGF ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1x DNase buffer (500ul) and 200U recombinant DNase I (20ul, Roche, 04716728001) at 37 C in a shaker set at 100 RPM ...
-
bioRxiv - Cancer Biology 2022Quote: ... DNA contamination was removed through treatment with recombinant DNaseI (Roche Diagnostics, #04716728001) for 15 minutes at RT and column purification using Qiagen RNeasy Mini kit (#74106) ...
-
bioRxiv - Neuroscience 2023Quote: ... DNA contaminations were eliminated with DNase I recombinant (#04716728001; Roche Applied Science) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... 10 µg RNA was treated with 10 units recombinant DNase I (Roche) for 1 h at 37 ° C ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... followed by PCR amplification with KAPA 2X Hi-Fi Hotstart Readymix (Roche, Cat. No. KR0370).
-
Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Expressed proteins were subsequently purified using a cOmplete His-Tag Purification column (1mL column, Roche) and SEC (S200 ...
-
bioRxiv - Cell Biology 2024Quote: ... supernatants were harvested and purified using His-Tag purification resin (Roche, cOmpelteTM, Cat No.5893682001), followed by size-exclusion chromatography in 20 M Hepes or Tris pH 8 ...
-
Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with the Complete His-Tag Purification Resin (Roche, Basel, Switzerland) that had been prewashed with Renz buffer ...
-
bioRxiv - Genomics 2024Quote: ... Hi-C libraries were prepared using the KAPA Hyper Library Prep Kit (Roche, cat#KK8502) or Arima Library Prep kit (cat# A303010 ...
-
bioRxiv - Bioengineering 2021Quote: ... Westernblot analysis of epitope-tagged PanK variants was performed with peroxidase-conjugated anti-HA antibody (Roche Diagnostics) and luminol-containing detection system ...
-
bioRxiv - Plant Biology 2024Quote: ... The HA-tagged proteins were immunoprecipitated using anti-HA antibody-coupled beads (anti-HA affinity matrix, Roche), while the GFP-tagged proteins were immunoprecipitated using anti-GFP affinity beads (SA070001 ...
-
bioRxiv - Microbiology 2021Quote: ... anti-human CD4 clone SP35 and anti-human CD8 clone SP57 (Roche, Basel, Switzerland), and anti-human CD20 clone L26 Dako Omnis (Agilent ...
-
bioRxiv - Biophysics 2020Quote: ... fibronectin from human plasma (Roche), the final concentration used for the experiments was 50 μg/ml (containing 2/3 of bovine and 1/3 of human FN) ...
-
bioRxiv - Cell Biology 2020Quote: ... Human fibronectin (Roche Diagnostics, 11051407001); Puromycin (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... and human insulin (11376497, Roche) were digested with recombinant WT or protease-dead (cf-E111Q ...
-
bioRxiv - Neuroscience 2020Quote: ... The resulting Hi-C library was amplified by PCR (KAPA Biosystems HiFi HotStart PCR kit, KK2502), and sequenced by Illumina 50 bp paired-end sequencing.
-
bioRxiv - Biophysics 2020Quote: ... and a biotin molecule (Supplementary Note 1) via PCR amplification with KAPA Hi Fi Polymerase (Roche) and nicking near the ends by the site-specific nicking enzyme Nt.BspQ1 (NEB) ...
-
bioRxiv - Biochemistry 2022Quote: ... lysed by sonication as described above and loaded onto the cOmplete His-tag purification column (Roche). The complex was eluted in 300 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
-
bioRxiv - Cell Biology 2024Quote: ... the cleared lysate was added to 1 mL cOmplete His-Tag Purification Resin (COHISR-RO, Roche) that had been pre-washed with buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... Hi-C next-generation sequencing (NGS) libraries were prepared using the KAPA HyperPlus Kit (KAPA Biosystems) according to instructions 129.
-
bioRxiv - Evolutionary Biology 2023Quote: ... the supernatant was further incubated with 25 μl of cOmplete His-tag purification resins (Roche, Switzerland) at 4 °C for 2 h ...
-
bioRxiv - Plant Biology 2020Quote: ... 4 µg recombinant NAA50 was incubated with 100 µM Acetyl-Coenzyme A (Roche) in a 2X acetylation buffer (50mM Tris HCl pH 7.5 ...
-
bioRxiv - Systems Biology 2021Quote: Recombinant proteins were purified using the Anti-Protein C Affinity Matrix (11815024001, Roche) on an ASPEC liquid handling instrument (Gilson) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The extracted RNA was then treated with DNase I recombinant RNase free (Roche) and the reverse transcription was done using the Super-Script IV (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: ... 100 μg of total RNA was incubated with recombinant DNase I (Roche, 04716728001) for 30 min at 37°C and the reaction was stopped by incubation at 75°C for 10 min ...
-
bioRxiv - Microbiology 2020Quote: ... Residual genomic DNA contamination was removed by treatment with recombinant DNAse I (Roche). cDNA was generated using Superscript II reverse transcriptase and Oligo d(T ...
-
bioRxiv - Neuroscience 2022Quote: Recombinant lentiviruses were produced by transfecting HEK293T cells using FuGENE®6 (Roche) with plasmids encoding viral enzymes and envelope proteins essential for packing of viral particles (pRSV-EV ...
-
bioRxiv - Immunology 2023Quote: ... Macrophages were polarized with 20 ng/mL recombinant IL-4 (Peprotech or Roche) or 100 ng/mL LPS (E ...
-
bioRxiv - Microbiology 2023Quote: DNase treatment was performed using recombinant RNase-free DNase I (Roche, Basel, Switzerland) according to the manufacturer’s protocols for 1 hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
-
bioRxiv - Microbiology 2023Quote: ... 1X antibiotic-antimycotic) with 100 U/mL recombinant interleukin (IL)-2 (Roche #11147528001) and activated or frozen in freezing media (90% FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... 12.5 mM EDTA) containing 100 μl proteinase K (recombinant PCR grade solution, Roche) was added to each sample and digested at 65 °C for 15 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... Embryos were hybridized with a digoxigenin (DIG)-tagged antisense mRNA probes detected by an anti-DIG antibody (Roche) and developed in NBT/BCIP (Roche) ...
-
bioRxiv - Biochemistry 2024Quote: ... HA- and GFP-tagged proteins were detected using horseradish peroxidase-conjugated monoclonal anti-HA (Roche, 3F10, 1:5,000) and anti-GFP (Roche ...
-
bioRxiv - Developmental Biology 2024Quote: ... antisense probes were synthesized from each plasmid using Sp6 or T7 polymerase and digoxigenin (DIG)-tagged nucleotides (Roche). Colorimetric and fluorescent in situ hybridizations were performed as described previously109 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Localization of HA tagged proteins were analyzed with an anti-HA tag antibody (Roche, clone 3F10, 1 : 500) and Alexa Fluor coupled secondary antibodies (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... 180 g/ml human transferrin (Roche), 5 ng/ml VEGF (PeproTech 450-32) ...