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Citations for Roche :
151 - 200 of 322 citations for Methacrylic acid N hydroxysuccinimide ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... pH 7.0 with 0.75 M 6-aminocaproic acid) with freshly added protease inhibitors (Roche). After resuspension ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Slides were rinsed in maleic acid buffer and blocked with 1% blocking reagent (Roche). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... 20mM β-glycerophosphate and a cOmplete™ EDTA-free protease inhibitor cocktail tablet added (Roche, cat n°11873580001). In case of HAP1 lysates ...
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bioRxiv - Microbiology 2022Quote: Bacterial DNA was extracted and purified using the High Pure PCR Template Preparation kit (Roche, cat. n° 11796828001) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... After two washes with cold PBS the cells were collected in the Lysis buffer (5 mM PIPES pH 7.5, 85 mM KCl, 0.5% NP40, 20 mM N-ethyl maleimide [NEM] and protease inhibitor cocktail [04693159001, Roche]) and incubated at 4°C for 10 minutes with rotation ...
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bioRxiv - Immunology 2021Quote: ... in combination with the N-gene (inhouse primer sets in multiplex PCR) on LightCycler® 480 (Roche Diagnostics).
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bioRxiv - Molecular Biology 2022Quote: ... with modifications - membranes were pre-hybridized in a volume of 0.2ml of pre-hybridization solution (5xSSC, 0.1% N-Lauroylsarcosine sodium salt, 1% SDS, 2% Blocking reagent (Roche)) for each 1cm2 of the membrane ...
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bioRxiv - Microbiology 2023Quote: ... 1 mM EDTA and 2 % N-lauroylsarcosine (w/v)) supplemented with Protease Inhibitors Cocktail (cOmplete ULTRA Tablets Roche). Bacterial cells were lysed with a M110-P microfluidizer (Microfluidics ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 5 mM N-ethylmaleimide and Complete protease inhibitor cocktail (Roche, one tablet/10 ml of buffer). HA-tagged ubiquitin was purified using Pierce anti-HA magnetic beads (clone 2–2.2.14) ...
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bioRxiv - Cell Biology 2024Quote: All qPCR reactions were performed with a LightCyler 1.3 Real-Time PCR system (Roche S/N: 140 6143). The MgCl2 concentration (1-3 mM ...
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bioRxiv - Plant Biology 2024Quote: ... RNA samples were separated on 1% agarose formaldehyde gels and transferred to Hybond-N membranes (Roche Molecular Biochemicals). Membrane hybridization was carried out overnight at 65°C using digoxigenin-labeled riboprobes corresponding to PVX CP sequences.
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bioRxiv - Cancer Biology 2024Quote: ... N-glycopeptides were enzymatically de-glycosylated and eluted from the beads with 5 U of PNGase F (Roche) in 100 mM NH4HCO3 at 37°C overnight ...
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bioRxiv - Immunology 2024Quote: ... colonic biopsies (n=3-4 per patient) were enzymatically digested using 0.5 Wünsch units/ml Liberase TM (Roche) and DNase I at 10µg/ml at 37°C (a protocol previously optimized in our lab)(43) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... mice (n=2 per group) were randomly assigned to receive either diazepam (synthesized by F. Hoffmann-La Roche) 0.3 ...
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bioRxiv - Cell Biology 2020Quote: ... [U-13C]palmitate was first non-covalently conjugated to ultra fatty acid free BSA (Roche) as previously described (Vacanti et al. ...
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bioRxiv - Pathology 2022Quote: ... 10 mM Okadaic acid) containing protease inhibitor cocktail (cOmplete ULTRA Tablets Mini EASYpack; Roche Diagnostics). Protein concentration was measured by BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Viral DNA was extracted using the Roche high pure viral nucleic acid kit (Roche, USA) according to manufacturer’s instructions and circular DNA was enriched by rolling circle amplification (RCA ...
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bioRxiv - Physiology 2022Quote: ... pyruvate supplemented with 2% Bovine Serum Albumin Fraction V Fatty acid free (Roche, USA/UK), 50 µM 2-mercaptoethanol ...
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bioRxiv - Pathology 2020Quote: ... RNA was extracted with the High Pure Kit Nucleic Acid Kit DNA-RNA (Roche Diagnostics) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% protease inhibitor cocktail set III ethylenediaminetetraacetic acid(EDTA)-free (Roche, Shanghai, China). Protein concentrations were determined using a Pierce BCA Protein Assay Kit (Lineage Cell Therapeutics ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and purified in large volume columns (High Pure Viral Nucleic Acid Large Volume Kit, Roche) using 2.5 ml of PB buffer ...
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bioRxiv - Genetics 2023Quote: ... 1% Triton X-100 and 1× cOmplete™ ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor (Roche). After removal of cell debris by centrifugation (twice 10 min ...
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bioRxiv - Pathology 2023Quote: ... viral DNA was isolated using the High Pure Viral Nucleic Acid kit (Roche Applied Science). Viral titers in terms of viral genome per milliliter (vg/mL ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids from the nasopharyngeal sample were extracted on the MagnaPure96 platform (Roche, Mannheim, Germany) using the MagNA Pure 96 DNA and Viral NA Large Volume Kit (Roche) ...
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bioRxiv - Microbiology 2022Quote: Prior to quantitative amino acid analysis Cel1cat was de-glycosylated using EndoH (Roche Diagnostics GmbH). The de-glycosylation was carried out in 20 mM MES ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 mM Okadaic acid) containing protease inhibitor cocktail (cOmplete ULTRA Tablets Mini EASYpack; Roche Diagnostics). The protein concentration was measured using BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The free fatty acids (FFA) levels were determined using a colorimetric assay (Roche, Mannheim, Germany). Cholesterol was determined by colorimetric assay (Erba Lachema ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM Tris-HCL pH 6.8 and 1x ethylenediaminetetraacetic acid (EDTA) free protease inhibitor (Roche) followed by incubation at 37°C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... all multiplexed single-cell libraries (n = 30) were quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche) and pooled in an equimolar ratio ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was incubated for o/n with 40μl of anti-HA agarose beads (rat anti-HA, 3F10 Roche) at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) 2-mercaptoethanol and 60 × 10−3 М n-Octyl-β-D-Glucopyranoside) containing protease inhibitor (Roche). Total protein was collected and incubated with anti-p75NTR (Alomone ...
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bioRxiv - Plant Biology 2021Quote: ... 20 μg of total RNA was separated on a denaturing 1.2% agarose gel and blotted on a Hybond-N+ (Roche) membrane ...
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bioRxiv - Microbiology 2020Quote: ... separated by electrophoresis in a 0.8% agarose gel and transferred onto a nylon membrane (Hybond N+, Roche Molecular Biochemicals). The membrane was hybridized with digoxigenin-labeled DNA probes synthesized with a PCR DIG probe synthesis kit (Roche Molecular Biochemicals ...
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bioRxiv - Developmental Biology 2020Quote: ... Whole cells extract was isolated using RIPA buffer (NaCl 150mM – Tris HCL pH7,35 50mM – DOC 1% – N-P40 1% – H2O) supplemented with protease inhibitor (04 693 116 001, Roche). Isolated protein concentration was determined using Bradford assay (500-0006 ...
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bioRxiv - Genetics 2020Quote: ... pellets were thawn and re-suspended in 400 μl of lysis buffer (50mM HEPES pH7.5, 150mM NaCl, 5mM MgCl2, 40mM N-Ethylmaleimide, EDTA-free protease inhibitor cocktail (Roche) and 2mM PMSF (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... with their respective dilution in 5% skimmed milk in PBS-tween 0.1% were used: anti-HA-HRP (3F10) (N°12013819001, Roche) 1/10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... hydrated through an alcohol series and boiled in CC1 (citric acid buffer, Roche Diagnostics, Basel Switzerland) for 1 hour (pH 6 ...
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bioRxiv - Genomics 2020Quote: ... concentrators and purified in large volume columns (High Pure Viral Nucleic Acid Large Volume Kit, Roche) using 10X (2.5 ml ...
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bioRxiv - Microbiology 2020Quote: ... or the MagNA Pure LC Total Nucleic Acid Isolation Kit (Cat. 03038505001, Roche Diagnostic, Basel, Switzerland) following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2021Quote: ... as described by (18)) with supplemented ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail tablets (Roche Diagnostics), 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acid extraction was performed using the MagNA Pure Compact DNA Isolation Kit I (Roche Diagnostics) on the MagNA Pure LC automated extractor ...
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bioRxiv - Microbiology 2021Quote: ... Total nucleic acid was extracted from samples with the MagNA Pure 96 system (Hoffmann-La Roche) using the MagNA Pure 96 DNA and Viral NA Small Volume Kit and eluted in a volume of 50μl Roche Tris-HCl elution buffer ...
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bioRxiv - Immunology 2020Quote: ... viral RNA was isolated from plasma using a MagNA PureCompact Nucleic Acid Isolation kit (Roche Diagnostics). Real-time RT-PCR was performed using a QuantiTec Probe RT-PCR kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... 2% Triton X-100) containing ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Roche cOmplete, Sigma Aldrich)] ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids were purified from whole blood or serum using the MagNA Pure 96 system (Roche) with the DNA/Viral NA 2.0 kit and the Viral NA Plasma external lysis S.V ...
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bioRxiv - Microbiology 2022Quote: ... Viral DNA was then extracted using the High Pure Viral Nucleic Acid Kit (Roche Applied Science) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... The genomic DNA was extracted from pools using High Pure Viral Nucleic Acid Kit (Roche, Germany) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... and viral genomes were purified with the High Pure Viral Nucleic Acid kit (Roche, Mannheim, Germany). Genome quantification of DENV ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 tetrazolium]-bis(4-methoxy-6-nitro)-benzene sulfonic acid hydrate (XTT)-based colorimetric assay (Roche) according to manufacturer-recommended protocols ...
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bioRxiv - Microbiology 2020Quote: ... Southern blotting was carried out using Hybond-N+ membrane (Amersham) and DIG High Prime DNA Labeling and Detection Starter kit II (Roche), following the indications of the manufacturer ...