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Citations for Roche :
1 - 50 of 322 citations for Methacrylic acid N hydroxysuccinimide ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... synthesized by IDT) with 66 mM digoxigenin N-hydroxysuccinimide ester (Roche) in 50 mM HEPES-NaOH ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) and protease inhibitor cocktail (Roche) on ice for 20 min ...
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bioRxiv - Genetics 2020Quote: ... acid-extracted histones were digested with Asp-N and Arg-C (Roche) and the resulting digests were analyzed by chromatography on an Ultimate 3000 nanoLC (Dionex ...
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bioRxiv - Biochemistry 2023Quote: ... uridine 5’-diphospho-N-acetyl-glucosamine (UDP-GlcNAc) and cytidine-5’-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) were obtained from Roche Diagnostics [UDP-Gal ...
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bioRxiv - Immunology 2021Quote: We used DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) (Roche) to introduce LPS or oxPLs into cells following the method by Zanoni et al (Zanoni et al. ...
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bioRxiv - Microbiology 2022Quote: ... 25 uL of N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate (DOTAP) (Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Microbiology 2022Quote: ... Two µg plasmid DNA and 25 µL DOTAP (N-[1-(2,3- Dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate) (Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Molecular Biology 2021Quote: ... lysate supernatant from cells expressing N-terminal 6-His-tagged proteins were incubated with nickel-nitrilotriacetic acid (Ni-NTA) cOmplete His-tag purification resin (Roche) for 1 h at 4° C ...
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bioRxiv - Cell Biology 2020Quote: ... MEFs were seeded subconfluently o/n (unless indicated otherwise) onto coverslips acid-washed and coated with human fibronectin (25 μg/ml, #11051407001, Roche), as described (Dimchev and Rottner ...
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bioRxiv - Bioengineering 2024Quote: ... sulfanilamide (Reagent I) and N-(1-Naphthyl)-Ethylenediamine in hydrochloric acid (Reagent II) from the “Nitrite/Nitrate Colorimetric Test” (Roche) kit (#11746081001 ...
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bioRxiv - Plant Biology 2022Quote: ... The products then transferred from the gel to Hybond N+ Nylon membrane (Amersham Biosciences) and detected using DIG Nucleic Acid Detection Kit (Roche Diagnostics) according to manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: ... The products were then transferred from the gel to the Hybond N+ Nylon membrane (Amersham Biosciences) and detected using DIG Nucleic Acid Detection Kit (Roche Diagnostics), according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Cationic lipid N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (Dotap, Liposomal Transfection Reagent, Cat#1202375, Roche, Nonnenwald, Penzberg, Germany) was used for the conjugation of phosphorothioated miRNAs and Dotap-formulated miRNAs were used for in vitro delivery of miRNAs ...
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bioRxiv - Immunology 2021Quote: ... N-Glycans were released by 10 U N-glycosidase F (Roche) digestion in 50 mM NH4HCO3 buffer pH 8.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... For N-glycan release 2.5 U N-glycosidase F (Roche, DE) were added and the resulting mixture was incubated overnight at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) (pH 8.0) and the presence of Complete® protease inhibitor (Roche). The preparation was centrifuged for 10 min at 2500 rpm and 4 °C and supernatant was transferred to a new tube and centrifuged for 40 min at 30,000 x g and 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) and protease inhibitor (Complete, Roche Applied Science)) for 2 h at 0°C (ref A) ...
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bioRxiv - Molecular Biology 2023Quote: ... 6.7 mM N,N-dimethylcyclohexylamine (DMCH) and EDTA-free complete protease inhibitor tablets (Roche). The mixture was incubated at 4°C with rotation for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... N-Glycosidase F (Roche, #11365185001) was diluted 1:10 in serum-free IMDM and incubated with the cells for two hours at 37°C prior to fixation ...
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bioRxiv - Cell Biology 2023Quote: ... N-Glycans were enzymatically released by peptide N-glycosidase F (E.C. 3.5.1.52; Roche Applied Science) digestion then purified by C18-Sep-Pak (Waters Corp. ...
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bioRxiv - Cancer Biology 2020Quote: ... Linoleic acid (C18) complexed with fatty acid free BSA (Roche 10775835001). PBS and BSA were used as the vehicle control in experiments containing C8 and C18 respectively ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were arrested at metaphase by incubating with colcemid (N-methyl-N-deacetyl-colchicine, Roche, 10295892001) for the last 14 hours before harvesting the cells ...
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bioRxiv - Microbiology 2022Quote: ... and N-Glycosidase F (11365177001, Roche) for 1h at 37°C prior to loading for SDS-PAGE.
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bioRxiv - Plant Biology 2024Quote: ... onto a Hybond–N membrane (Roche). The membrane was auto-crosslinked at 120,000 μJ in a Stratalinker 1800 (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acids were extracted using the High Pure Viral Nucleic Acid Kit (Roche) according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2020Quote: ... peptide-N-glycosidase F (PNGase F) (Roche), neuraminidase (Roche) ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... (2 mg/kg; atezolizumab, Roche; n = 12). Group 3 received anti-TGF-β I.P ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM amino acids (Roche), 1 nM pY71sfGFP plasmid encoding superfolder GFP (M ...
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bioRxiv - Cell Biology 2020Quote: Cell viability was determined by MTT (C, N-diphenyl-N′-4,5-dimethyl thiazol-2-yl tetrazolium bromide) assay (Roche, Switzerland), a standard colorimetric assay which uses the metabolic reduction of the tetrazolium salt to form the colored formazan product [33] ...
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bioRxiv - Neuroscience 2024Quote: ... and Str tissue from old (N =6 wt, N =8 tgNM) mice were homogenized in RIPA buffer supplemented with protease inhibitors (Roche) and cell extracts clarified by centrifugation at 13000g for 30 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... gel bands corresponding to the LRP1 protein were excised and the N-glycans were attained from an overnight in-gel N-glycan release with 2U PNGaseF (Roche) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... T the gels were electroblotted on positively charged nylon membranes (Amersham Hybond-N RPN 203 N) followed by UV crosslinking and hybridised in DIG EasyHyb buffer (Roche) at 68 °C with gentle agitation overnight ...
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bioRxiv - Immunology 2023Quote: ... fatty acid free (Roche, Basel, Switzerland), 50 μmol/L 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Sera were analysed for antibody directed against the SARS-CoV-2 Spike protein receptor-binding domain (S-RBD) and nucleocapsid (N) using an established automated electrochemiluminescence assay (Elecsys Anti-SARS-CoV-2 S and N, Roche diagnostics) [54] ...
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bioRxiv - Molecular Biology 2020Quote: ... then transferred to a Hybond-N+ nylon membrane (Roche) and hybridized with Biotin-labelled DNA probes crossing junction site B ...
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bioRxiv - Biochemistry 2021Quote: ... Peptide N-glycosidase F (PNGaseF) was purchased from Roche Diagnostics (Mannheim ...
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bioRxiv - Microbiology 2021Quote: ... were used: anti-HA-HRP (3F10) (N°12013819001, Roche) 1/10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-DIG-POD (Roche Cat. N: 11207733910, 1:500) and Anti-FITC-POD (Roche Cat ...
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bioRxiv - Genomics 2021Quote: Genotyping of F2 umbilical cord tissues (n = 227) and F1 saliva samples (n = 189) was performed with targeted DNA sequencing (Roche: SeqCap EZ Choice). The region genotyped for the tar-RNAseq samples overlapped the region of the wht-RNAseq samples (1.4 Mb ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1% deoxycholic acid and protease inhibitors (Roche), and thrice with lysis buffer without detergent ...
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bioRxiv - Cell Biology 2023Quote: ... containing 0.5% fatty acid-free BSA (Roche), 10 mg ml-1 Collagenase D (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% deoxycholic acid with protease inhibitors (Roche). The lysates were subject to anti-GFP immunoprecipitation using GFP-Trap beads or anti-Myc immunoprecipitation using Myc-Trap beads (ChromoTek) ...
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bioRxiv - Plant Biology 2023Quote: ... ethylenediaminetetraacetic acid free protease inhibitor mixture (Roche) and phosphatase inhibitor mixture (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... each fatty acid was first conjugated to 10% fatty-acid-free BSA (bovine serum albumin fraction V) (#10735086001, Roche) for 1 hour at 50°C in a 50:50 volumetric ratio ...
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bioRxiv - Developmental Biology 2022Quote: ... and Anti-FITC-POD (Roche Cat. N. 11426346910, 1:500).
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bioRxiv - Microbiology 2023Quote: ... destained and digested with Asp-N (Roche, 20 ng/μL) overnight at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... N-trimethyl-ammonium methylsulfate (DOTAP) (Roche Applied Sciences, Indianapolis, IN), which showed high efficacy for in vivo transfection [31] ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from 253 resuspended vaginal swab samples using the MagNA Pure Compact Nucleic Acid Isolation Kit (Roche) [33] ...
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bioRxiv - Biochemistry 2024Quote: ... The synergistic effect was determined based on the amount of acetic acid released using an acetic acid assay kit (Roche).